Two sorghum(Sorghum bicolor L. Moench cv. Hwanggeumchal-susu and Miryang 3) samples were milled using different milling methods, and their physicochemical properties were tested. Particle size was classified into five groups such as pin mill and low temperature-microparticulation(LTM; 10, 000, 20, 000, 30, 000, and 40, 000 rpm). The water absorption index (WAI), water solubility index(WSI), and a rapid Visco analyzer(RVA) were used to examine particle size distribution and color differences. Particle size of sorghum flour prepared using LTM was lower than that prepared using a pin mill. Particle size was further reduced by successive dry milling of the LTM flour. Lightness of colored pigments increased when particle size decreased. The WAI of Miryang 3 pin milling(M1) flour was the lowest after LTM, and WSI was higher in the order of M2, M3, M4, and M5. LTM sorghum flour had significantly higher pasting viscosity, as determined using a rapid Visco analyzer. LTM Miryang 3 sorghum flour(M2～M5) flour showed lower breakdown viscosity and higher final viscosity compared to those of M1 flour, resulting in an increased setback value.

The sorghum (Sorghum bicolor (L.) Moench) variety ‘Sodamchal’ was developed and registered by Department of Sothern Area Crop Science, National Institute of Crop Science (NICS) in 2013. This variety was developed from the cross between ‘Hwanggeumchal’ with brown grain and ’Jungmo4001’ with semi-dwarf trait. ‘Sodamchal’ is the first semi-dwarf stature with waxy endosperm ever developed in Korea. The yield performance of ‘Sodamchal’ was 2.95 MT/ha in local adaptability test for 2 years. It had 91 cm culm length, which was 58 cm shorter than that of ‘Hwanggeumchal’, 26.3 cm panicle length, 22.0 mm thickness of culm, 21.1 g in 1,000 grain weight. It could be reduce the lodging problem and also adapted to mechanized cultivation. ‘Sodamchal’ produces high quantities of polyphenol and radical scavenging activitiy with diphenyl-picrylhydrazyl (DPPH), 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) in pigmented testa. Above all ‘Sodamchal’ is suitable for use as a parental source for the development of improved pollinator parent lines for developing semi-dwarf sorghum with waxy endosperm and antioxidant activity. (Variety registration No. 6244)

This study was carried out to identify the optimum seeding dates for selecting a double-cropping system and to assess the effect of polyethylene film mulching on the yield of Proso millet. Seeds of the varieties Hwanggeumgijang and Ibaegchal were sown in Miryang on five different dates: 1st (May 25), 2nd (June 15), 3rd (June 25), 4th (July 5), and 5th (July 15), with and without polyvinyl mulching. The varieties have different characteristics, for example, Hwanggeumgijang is an early-maturing type and more sensitive to temperature, whereas Ibaegchal is a medium-maturing type and more sensitive to the duration for which it is exposed to sunlight. Late-sown Hwanggeumgijang had a short heading date from seeding and required a low accumulated temperature. It also had a shorter period of heading, a shorter culm length and a shorter diameter of stem. In contrast, it had had a higher number of ears per m2 although similar ear length and similar 1000-grain weight. The yield potential of Hwanggeumgijang was found to decrease at a late seeding date. In particular, it significantly decreased at the seeding date of July 15. In the case of cultivation with polyvinyl mulching, the period of heading was shorter by 2–4 days and the yield potential was increased by approximately 12–32%. The length and diameter of culm in Ibaegchal were slowly decreased, but the length of ear, the 1000-grain weight and the yield potential were similar for all seeding dates (except July 15) and cultivation with and without mulching. When sown late, the length and diameter of the culm of Ibaegchal very rapidly decreased by the July 15 seeding date. The protein content of Ibaegchal was higher but the amlyose content of Ibaechal was lower compared to Hwanggeumgijang. At late seeding dates, the protein contents of the two varieties increased but the amylose contents were similar.

‘이백찰’은 제주도에서 수집된 재래기장을 기본집단으로 하여 분리육종법을 통해 국립식량과학원에서 2011년 육성된 신품종으로 주요특성과 수량성을 요약하면 다음과 같다. 출수기는 표준품종인 ‘황금기장’보다 보통기 재배에서는 13일 정도 늦은 중만생종으로 생육일수는 109일 정도이며 기본영양생장 기간은 길다. 간장은 140 cm 정도로 ‘황금기장’에 비해 약간 크고, 도복에 강한 특성을 나타낸다. 이삭은 심한 하수형이며 이삭길이는 40 cm로 매우 길다. 종실색은 흰색이고, 낟알은 노란색이며, 배유의 아밀로스 함량이 8.2%로 찰기장에 해당하며, 낟알의 무기성분 중 칼슘 4.1 mg/100g, 마그네슘 38.0 mg/100g 으로 비교적 함량이 높으며 DPPH 소거 항산화활성이 ‘황금기장’에 비해 높은 특성을 가지고 있다. 수량은 지역적응시험 보통기재배 평균 2.29 MT/ha로서 표준품종보다 15% 정도 증수되는 다수성이고, 기본영양생장 기간이 길기 때문에 중·남부 평야지역에 적응성이 높으며, 내도복 직립 초형으로 기계화 재배에 유리한 우수한 품종이다.

Koji (Aspergillus oryzae) is used to ferment crude cereals of wheat to make a traditional alcoholic drink called Makkolli and industrial materials. It’s quality varies depending on the wheat quality. Four domestic wheat varieties (Kosomil, Jokyungmil, Geumgangmil, Baegjungmil) were characterized. They were found similar in pH (6.02 to 6.08) and total acid (0.105 to 0.120%) contents. However, amino acid content of Gemgangmil was the highest (4.46%) and that of Baegjungmil was the lowest (3.72%). The total bacillus number was highest in Kosomil (333×103CFU/ml) and lowest in Gemgangmil (60×103CFU/ml). On the other hand, the fungus number was 47×105CFU/ml in Gemgangmil and the other varieties had similar quantity. The content of Alpha-amlyase was the highest (500.01unit/g) in Kosomil followed by Jokyungmil and Gemgangmil, and the lowest was in Baegjungmil (353.32unit/g). The content of Glucoamlyase was the highest in Geumgangmil (5105.0unit/g) followed by Jokyungmil and Kosomil, and the lowest was in Baegjungmil (3880.0unit/g). Acid protease was the highest in Kosomil (3515.15unit/g) followed by Geumgangmil and Baegjungmil, and the lowest in Jokyungmil (1280.5unit/g). From the result, Koji made from Kosomil was found to be of superior quality.

Sorghum seed is traditionally used as secondary food sources in addition to rice in Korea. While the hypoglycemia regulating phytochemicals have been found in sorghum seed, peptides related with hypoglycemia never been studied before. To obtain the peptide characteristics and the specifically high-expressed peptides in hypoglycemic sorghum seed, peptide profiles of seven hypoglycemic and five nonhypoglycemic sorghum lines bred in RDA were determined using surface-enhanced laser desorption/ionization time-offlight mass spectrometry (SELDI-TOF MS). The twelve sorghum lines exhibited 104 peptides on CM10 protein chip array (weak cation exchange) and 95 peptides on Q10 (weak cation exchange) in the molecular mass range from 2,000 to 20,000 Da. Heat map via supervised hierarchical clustering of the significantly different peptides (p < 0.01) in peak intensity among the 12 lines effectively revealed the specifically upregulated peptides in each line and distinguished between 7 hypoglycemic and 5 non-hypoglycemic lines. Through the comparison with hypoglycemic and non-hypoglycemic lines, 10 peptides including 2231.6, 2845.4, 2907.9, 3063.5, 3132.6, 3520.8, 4078.8, 5066.2, 5296.5, 5375.5 Da were specifically high-expressed in hypoglycemic lines at p < 0.00001. This study characterized seed peptides of 12 sorghums and found ten peptides highly expressed for hypoglycemic sorghum lines, which could be used as peptide biomarkers for identification of hypoglycemic sorghum.

Wheat is one of the most important food crops in the world and provides 20% of nutritional requirement to the consumers in developing countries. The consumption per capita of wheat is persistent but the self-sufficiency has decreased owing to the less competitive world market and double cropping system with rice in Korea. This study was conducted to obtain baseline data on the selection lines with early maturity and good agronomic traits. Geumgangmil, a leading Korean variety, was crossed with CIMMYT’s line, and a DH (double haploid) line was released using pollens of Maize in Mexico. The 71 inbred lines were grown in fields at NICS, Miryang. The culm length of inbred lines were normally distributed from 37cm to 106cm, though that of the P1 (Geumgangmil) was 71 and P2 (CIMMYT’s Line) was 77cm. Especially, some lines were very short and measured below 50cm. The spike lengths of most of the lines were longer than that of P1 (7.5cm), but were normally distributed. The number of spikelet was less than that in P2 (19ea.), but was more than P1(10ea.). The heading day of most of the lines was between P1 (11th April) and P2 (25th April) and the maturity day of the lines was similar to P1 (1st June) but 5 lines showed 2~6 days shorter maturity period than that of P1. However, 20 lines were found to have 2~10 days longer maturity period than that of P2.

‘삼다메’는 국립식량과학원 기능성작물부에서 2010년도에 육성한 중생종이며 메성으로 혼반에 적합한 품종이다. ‘삼다 메’의 잎은 녹색이나, 생육후기에는 황록색을 띤다. 분얼경은 직립으로 도복에 강하다. 이삭의 형태는 원추형으로 이삭의 착립밀도가 조밀하고 탈립이 안되며 낟알은 황색으로 메성이 다. ‘삼다메’는 밀양, 원주, 신안의 보통기 표준재배에서 평균 출수기가 8월 10일로 ‘황금조’보다 12일 늦은 중생종이다. 생 육일수는 보통기 재배에서 101일로 ‘황금조’에 비하여 약13 일 길고, 성숙일수는 6일 정도 길다. 간장은 보통기 보비재배 에서 130 cm로 ‘황금조’에 비해 18 cm 정도 크고 현곡천립 중은 ‘황금조’와 같다. 포장에서의 조도열병, 세균성줄무늬병 은 ‘황금조’에 비해 강한 편이나 속깜부기병은 다소 높게 발 생하였다. 조명나방 및 노린재류의 해충에는 ‘황금조’와 같이 약한 반응을 보였다. ‘황금조’에 비하여 Ca 및 항산화활성이 높은 기능성을 가지고 있으며, 수량은 2.42 MT/ha로 ‘황금 조’에 비해 25% 증수하였다.

SELDI-TOF MS를 활용한 저분자 펩타이드 분석을 위해 서는 분석시료에 대한 최적화 분석조건을 확립하는 것이 필수적으로 선행되어야 한다. 본 연구는 수수 종자 내 존재하는 10 kDa 이하의 저분자 펩타이드를 프로파일링하기 위하여 활용된 SELDI-TOF MS의 최적화 분석조건을 확립하는데 있다. 분석조건은 (1) 프로테인 칩: CM10(weak cationexchanger), Q10(strong anion exchanger), (2) 바인딩 버퍼의 희석배수: 1/2, 1/5, 1/10, 1/20, 1/50, 1/100, 1/200, (3)Q10의 바인딩 버퍼 강도: 10 mM, 100 mM, (4) 단백질 추출버퍼: sodium borate, sodium borate + acetone, phenol,TCA 버퍼로 하였다.1. 바인딩 버퍼의 희석배수는 CM10과 Q10 모두 1/20과1/50이 최적화로 나타났다.2. Q10의 바인딩 버퍼 강도는 농도가 약한 10 mM에서 더많은 피크가 검출되었다.3. SELDI-TOF MS 분석에 적합한 수수 종자단백질 추출버퍼로는 2~10 kDa 범위에서는 sodium borate 버퍼와10~20 kDa 범위에서는 phenol 버퍼로 분석되었다.

‘경관2호’는 국립식량과학원 기능성작물부에서 2008년 하계에 진주 재래조를 수집하여 2008/2009년 동계온실에서 두 번 집단을 양성하여 순계분리한 도복에 강하며 찰성의 특성을 지닌 혼반 및 경관용 품종으로 2009년에 육성되었다. ‘경관2호’의 잎은 녹색이나, 생육후기에는 자색으로 변하며, 분얼경은 직립으로 도복에 강하고 안토시안 색소 발현이 있다. 이삭의 형태는 원추형이며 이삭의 착립밀도는 ‘황금조’와 비슷하며 탈립이 잘되는 편이다. 낟알은 황색이며 찰성이 있다. ‘경관2호’의 생육일수는 보통기 표준재배에서 127일로 ‘황금조’보다 25일 늦고, 성숙일수는 11일 정도 길다. 간장은 보통기 표준재배에서 128 cm로 ‘황금조’에 비해 23 cm 정도 크나 포장 도복에 강하다. 현곡천립중은 2.1 g으로 ‘황금조’에 비해 가벼운 편이다. 포장에서의 조도열병과 세균성줄무늬병 및 벼줄무늬잎마름병 발생이 없었으나 조명나방 및 노린재류에는 약하다. 수량은 보통기 표준재배에서 2.00 MT/ha로 ‘황금조’에 비해 5% 정도 적다.

This study evaluated the effect of organic fertilizer on the yield of sesame and the movement of nitrogen and carbon in soil under mulching cultivation. The seven fertilizer treatments investigated are as follows: 1) 8-4-9 kg N-P2O5-K2O 10a-1-control, 2) 80% of control treatment N from mixed expeller cake (MEC)-MEC 80%, 3) 100% of control treatment N from MEC-MEC 100%, 4) 80% of control treatment N from mixed organic fertilizer (MOF)MOF 80%, 5) 100% of control treatment N from MOF-MOF 100%, 6) 80% of control treatment N from latex coated urea complex fertilizer (LCU)-LCU 80%, and 7) no nitrogen. The soil mineral N available in the applied organic MEC and MOF fertilizers was less than the available N in the applied urea fertilizer during the early growth stage of sesame but the amount was observed to increase with time. The concentration of nitrate-N in leached solution 35 days after application was highest at 75 mg l-1 in the control, followed in descending order by MEC 100%, MOF 100%, LCU 80%, MOF 80% and MEC 80%. The concentration of nitrate-N in the leachate in all treatments was observed to rapidly decrease 45 days after fertilizer application and recorded at less than 20 mg l-1 65 days after application. The concentration of dissolved organic carbon in the leached solution and organic carbon in the soil of the organic fertilizer plots was higher than in the urea- and LCU-fertilized plots during the whole growing period. The yields of sesame applied with MEC and MOF organic fertilizers were 2 to 7% higher than those fertilized with urea. The amount of retained mineral N or organic carbon in the soil is higher in the LCU and organic fertilizer plots than in the urea-fertilized treatments, which resulted in the reduction of nitrate leaching, increasing N efficiency and sesame yield.

This study was conducted to evaluate the effect of slow release fertilizers (SRF), crotonylidene diurea (CDU) and latex coated urea (LCU), on nitrogen (N) use efficiency (NUE) and nitrate-N leaching in a silty clay loam soil under polyethylene film mulching (PFM) for sesame cultivation. In PFM plot, concentrations of NO3-N and NH4-N in SRF applied soils were less than that in the urea plot during the whole growing period. However, NO3-N and NH4-N in all the non-mulched plots (NM) were not significantly different. Urea-N in soil treated with SRF was higher than urea plot until 50 days after application and was comparable in all the treatments after 50 days. NO3-N concentrations in leached solution in 21 days after urea fertilization in PFM and NM were 26 mg L-1 and 83 mg L-1 , respectively. However, NO3-N in leached solution at applied CDU and LCU was less than that of urea similar to nitrate concentration in soil. NO3-N in leached solution in applied CDU and LCU in 44 days after application was about 25% lower than that urea plot and PFM, while the NO3-N concentration of CDU and LCU treatment in NM did not changed. Application of SRF increased the yield of sesame and N recovery compared to urea and there was a little difference between SRF and N levels. In conclusion, application of 80% N level with SRF increased N recovery and reduced nitrate leaching without reduction of yields compared with urea application.