The present study was conducted to investigate effects of rabbit meat extract on energy metabolism and muscle differentiation in C2C12 myotubes. Water extract of rabbit meat (10, 50, 100, and 200 μg/ml) was used to treat differentiated C2C12 cells. Reverse transcriptase polymerase chain reaction (RT-PCR) and western blot analysis were used to determine mRNA or protein levels of energy metabolism-related genes. Total adenosine triphosphate (ATP) content was also measured. Treatment with rabbit meat extract significantly increased expression levels of muscle differentiation markers (myogenin and myosin heavy chain) and mitochondrial biogenesis regulators (PGC1α, NRF1, and TFAM) in C2C12 myotubes compared to non-treated control. Additionally, rabbit meat extract activated phosphorylation of AMPK and acetyl-coA carboxylase (ACC). Rabbit meat extract significantly increased ATP contents in myotubes. These results suggest that rabbit meat extract has the potential to improve energy metabolism in skeletal muscles.

국립원예특작과학원에서는 밝은 화색과 안정적인 화형의 생 육이 우수한 빨간색 스탠다드 장미 품종을 육성하기 위해 진한 적색 스탠다드 장미 품종 ‘엔드리스러브(Endless Love)’를 모 본으로, 꽃잎수가 많고 안정적으로 가시가 적은 밝은 노란색 ‘페니레인(Penny Lane)’ 품종을 부본으로 인공교배하였다. 37 개의 교배실생을 양성해 1, 2, 3차에 걸친 특성검정 및 현장실증 을 통해 꽃이 크고 화형이 안정적이며, 재배안정성 및 생산성, 절화특성이 우수한 ‘원교 D1-390’을 최종 선발하였다. 2023년 ‘루비레드(Ruby Red)’로 명명하여 국립종자원에 품종보호출원·등록되었다. ‘루비레드’ 품종은 밝은 적색(R53C)을 가졌으 며, 꽃잎수가 32.8매, 화폭과 화고는 각각 10.9, 5.9cm로 대조 품종보다 크다. 절화장은 평균 71.7cm, 절화수명은 약 16.7일, 수량은 연간 168대/m2로 대조품종인 ‘레드스퀘어(Red Square)’ 대비 절화장이 길고 절화수명도 2배 이상 길며, 수확량도 1.4배 우수하다. 2023년 국내 육성 장미 품종 서울식물원 관람객 대상 공동평가회에서 스탠다드 장미 중 우수한 평가를 받았으며, 현 장 실증 결과 농가별로 균일하고 우수한 수량과 절화품질을 보 였다. 절화용 장미 ‘루비레드’ 품종은 밝은 적색과 우수한 화형 을 가지는 품종으로 해외 대체 품종으로 국내에서 많이 재배될 것으로 기대된다.

본 연구를 통해 국내 미기록속인 그물나비속(Neope)의 검은눈그물나비(Neope muirheadii)를 처음으로 보고한다. 이 종의 채집지 정보와 간단 한 논평을 포함하여, 성충과 생식기의 사진을 함께 제시한다.

본 연구는 서바이벌 게임에서 맵의 디자인 요소를 분석하고 표준화하는 것을 목표로 했다. 상 용 게임 10개를 조사하여 네 가지 주요 맵 유형을 파악하였고, 이를 바탕으로 유니티 에디터를 사용해 네 가지 유형의 맵을 개발 및 구현하였다. 무작위 위치에 장애물을 스폰하고, 타일 재 배치 시스템을 통해 맵을 무한대로 재배치할 수 있도록 했다. 또한, Update() 메서드를 조정해 타일 변위 오류를 해결했다. 마지막으로, ML 에이전트를 사용해 맵을 테스트했으며, 에이전트 가 환경을 탐색하고 장애물과 적대 대상을 인식 및 공격하며 각기 다른 네 가지 맵에서 효과 적으로 대응하는 모습을 확인했다.

Sweetpotato (Ipomoea batatas L.) is a globally important food crop that is susceptible to infestation with the root-knot nematode, Meloidogyne incognita, which causes substantial crop losses. Previous transcriptomic and proteomic analyses identified several genes that displayed differential expression patterns in susceptible and resistant cultivars in response to root-knot nematode (RKN) infection. As a result of previous study, RKN infection was confirmed in the RKN-susceptible sweetpotato cultivar Yulmi. Transcriptome analysis confirmed that among the genes that respond in this process, there are many genes related to ethylene biosynthesis. Therefore, in this study, we focused on the ACC oxidase (ACO) gene, the final enzyme of ethylene biosynthesis, and analyzed the expression patterns under various abiotic stress conditions. Using transcriptome data from our previous study, various expression changes in the four ACO genes used in this study were confirmed during RKN infection. The expression of G25011|TU41034 decreased during RKN infection compared to the untreated control, while the expression of G31097|TU51009, G28360|TU46486, and G15447|TU25395 genes increased in the early stages of RKN infection. Expressions of four ACO genes in leaves of sweetpoato were investigated under abiotic stress conditions such as wounding, high salinity, dehydration, and low temperature stress treatment. Expression of the G25011|TU41034 was significantly increased under abiotic stress conditions except low temperature. G31097|TU51009 was hardly expressed under abiotic stress conditions. Although the expression pattern of G28360|TU46486 and G15447|TU25395 was slightly different depending on the type of abiotic stress, an overall increase in expression was observed. It is expected that this study will be used as basic data on how ethylene biosynthesis responds not only to nematode infection but also to various abiotic stress conditions and will be helpful in functional studies of various crops.

InP quantum dots (QDs) have attracted researchers’ interest due to their applicability in quantum dot light-emitting displays (QLED) or biomarkers for detecting cancers or viruses. The surface or interface control of InP QD core/ shell has substantially increased quantum efficiency, with a quantum yield of 100% reached by introducing HF to inhibit oxide generation. In this study, we focused on the control of bandgap energy of quantum dots by changing the Zn/(In+Zn) ratio in the In(Zn)P core. Zinc incorporation can change the photoluminescent light colors of green, yellow, orange, and red. Diluting a solution of as-synthesized QDs by more than 100 times did not show any quenching effects by the Förster resonance energy transfer phenomenon between neighboring QDs.

Background: Aflatoxin B1 (AFB1) is a toxic metabolite generated by Aspergillus species and is commonly detected during the processing and storage of food; it is considered a group I carcinogen. The hepatotoxic effects, diseases, and mechanisms induced by AFB1 owing to chronic or acute exposure are well documented; however, there is a lack of research on its effects on the intestine, which is a crucial organ in the digestive process. Dogs are often susceptible to chronic AFB1 exposure owing to lack of variation in their diet, unlike humans, thereby rendering them prone to its effects. Therefore, we investigated the effects of AFB1 on canine small intestinal epithelial primary cells (CSIc). Methods: We treated CSIc with various concentrations of AFB1 (0, 1.25, 2.5, 5, 10, 20, 40, and 80 μM) for 24 h and analyzed cell viability and transepithelial-transendothelial electrical resistance (TEER) value. Additionally, we analyzed the mRNA expression of tight junction-related genes (OCLN, CLDN3, TJP1, and MUC2), antioxidant-related genes (CAT and GPX1), and apoptosis-related genes (BCL2, Bax, and TP53). Results: We found a significant decrease in CSIc viability and TEER values after treatment with AFB1 at concentrations of 20 μM or higher. Quantitative polymerase chain reaction analysis indicated a downregulation of OCLN, CLDN3, and TJP1 in CSIc treated with 20 μM or higher concentrations of AFB1. Additionally, AFB1 treatment downregulated CAT , GPX1, and BCL2. Conclusions: Acute exposure of CSIc to AFB1 induces toxicity, and exposure to AFB1 above a certain threshold compromises the barrier integrity of CSIc.

The large copper butterfly Lycaena dispar (Haworth, 1803; Lepidoptera: Lycaenidae) has been categorized as a near-threatened species (NT) in South Korea from 2012 mainly due to limited distribution. In this study, we visited 36 sites spread across all South Korean provinces to verify the distributional range of the species and sequenced mitochondrial COI for 53 individuals from nine sites. We observed L. dispar at 15 sites in six provinces, including the two previously known provinces, indicating a southward range expansion. The in-field monitoring and genetic data collectively suggested that L. dispar does not have a limited distribution nor is it isolated, indicating that it should be reclassified as less vulnerable. Our study demonstrates that the combination of field and genetic data can provide a more reliable assessment of the stability of a species.

The clearwing moth, Synanthedon bicingulata (Staudinger, 1887), is a pest that infests various species of cherry trees. However, genetic information regarding the genus Synanthedon including S. bicingulata, is limited. In this study, we sequenced a complete mitochondrial genome (mitogenome) of the species. The 16,255 bp of S. bicingulata mitogenome differs from the typical gene arrangement formed in Lepidoptera: trnQ-trnS2-trnM-trnI arrangement between the A+T-rich region and the ND2 junction. Moreover, the genome has untranslated repetitive sequences in the intergenic space between lrRNA and trnV, as well as the CGA start codon in COI and the TTG start codon in ATP8. Similar observations are noted in species belonging to the tribe Synanthedonini within the genus Synanthedon.

Tropilaelaps mercedesae Anderson and Morgan, 2007 (Acari: Laelapidae) is a serious ectoparasite of the brood of several honey bee species. Among the four recognized species of Tropilaelaps, Korean population was renamed as T. mercedesae from T. clareae on the basis of morphological evidences and genetic data. In this study, we report the complete mitochondrial genome (mitogenome) sequence of T. mercedesae. The 15,119-bp long mitogenome has an identical gene arrangement to that of Chinese sample reported previously. Comparison of two geographic samples showed COII, ND5, ND4, ND6, CytB, and ND1 to have higher number of variable sites than COI, which is often used for population-level study, suggesting these genes to have potential usefulness for population genetic study. The mitogenome sequence of T. mercedesae from Korea could be useful for species identification for geographic samples, trace of the origin of local populations, and illustration of evolutionary distinction among Tropilaelaps species either using part of or whole genome.

전북특별자치도 수박(Citrullus lanatus (Thumb.) Matsum. et Nakai) 재배면적은 2,235ha이며, 익산과 정읍은 주요 수박 재배 지역이다. 본 연구는 수박에서 발생하는 해충 목록 작성을 위한 기초자료 구축 을 목적으로 최근 20년간 보고된 문헌자료를 조사하였다. 문헌조사에 따르면, 수박에서 발생하는 주요 해충은 총 7목, 11과, 22종으로 정리되었다. 이 중 점박이응애(Tetranychus urticae Koch), 목화진딧 물(Aphis gossypii Glover), 아메리카잎굴파리(Liriomyza trifolii Burgess) 등이 우점해충으로 다수의 문헌 을 통해 확인되었다. 그 외 파밤나방(Spodoptera exigua Hübner), 꽃노랑총채벌레(Frankliniella occidentalis Pergande), 목화바둑명나방(Diaphania indica Saunder)등의 해충들이 최근 연구로부터 추가되었다. 본 연구 결과를 기반으로 앞으로 수박 해충에 대한 현장 조사 계획을 수립하고, 적합한 방제 전략을 세우 고자 한다.

Spodoptera eridania and S. ornithogalli (Lepidoptera: Noctuidae), which are polyphagous pests that damage various crops such as tomatoes and beans are regulated quarantine species that are highly likely to invade South Korea. Therefore, it is crucial to promptly and accurately identify the presence of S. eridania and S. ornithogalli in crop fields to effectively eradicate as a regulated quarantine species. In this study, we developed a loop-mediated isothermal amplification (LAMP) assay, which allows for rapid in-field identification. To develop the LAMP　assay, we selected target species-specific genomic regions from the whole-genome sequences of one target and 13 other lepidopteran species. We validated each five and six primer sets that consistently produced positive reactions in S. eridania and S. ornithogalli, respectively. To test the sensitivity of the each locus, LAMP reactions were performed using various reaction times using crude DNA, which was extracted from various types of adult tissues. All sensitivity tests were also successful.

코로나바이러스감영증-19 사태가 전세계를 강타하면서, 우리는 기존과 다르게 변해가는 일상 에 적응하는 뉴노멀 시대를 맞이 하였다. 코로나19 이후, 방송, 강의분야 등 문화예술계에도 비대면 문화가 급속도로 확산되고, 방송업계는 랜선방청제도를 도입하여 화상시스템을 통한 온 라인 실시간 방청을 제공하여 관객의 참여를 유도하고 있으며, 강의행사 분야도 화상회의 시 스템을 활용한 진행과 유튜브 플랫폼을 활용한 실시간 송출의 비대면 형태로 진행되고 있다. 이러한 비대면 참여환경은 물리적 스테이지의 한계, 음향 촬영 구도의 아쉬움, 현장감, 생동감 등 몰입적 측면에서의 한계가 존재하고, 언택트 방송 및 공연의 경우 음향과 촬영구도가 아쉬 운 부분으로 꼽히며 현장과의 괴리감, 집중하고 싶은 장면을 선택하여 볼수 없다는 한계도 지 닌다. 비대면 강의 경우에도 일상에서 쉼 없이 이어지는 비대면 참여 환경에 ZOOM Fatigue 라는 신조어가 등장할 만큼 비대면 환경에서의 집중력과 흥미를 잃어가는 현상이 나타나고 있 다. 이처럼 코로나19로 인한 변화가 일상화되면서 인류의 역사는 코로나19 (BC, Before Corona), (AC, After Corona) 나뉠 것이라는 예측이 일기도 한다. 무엇보다 코로나19로 인 해 인류의 정치, 경제, 사회, 문화, 교육등 모든 영역이 대변화를 맞은 가운데 가장 큰 변화는 물리적 접촉이 최소화되면서 Untact문화가 확산됐다는 점이다.

문화유산은 조상들의 문화중에서 후손들에게 물려줄 만한 가치가 있는 것을 말한다.[1] 우리의 문화유산을 우리의 역사, 정체성, 그리고 자부심을 담고 있으며, 이를 통해 우리는 과거의 삶 과 지식, 그리고 경험을 재조명하고 이해할 수 있다. 우리의 문화유산을 보전하고 전파하는 것 은 매우 중요하다. 이를 위해 우리는 문화콘텐츠와 IT기술의 융합적인 미디어아트를 활용하여 이를 알리고자 한다. 이러한 미디어 아트는 국내외 관광객들에게 우리의 문화유산을 소개하는 데 더욱 효과적이다. 전남의 3대 별서정원은 이러한 문화유산 전파의 중요한 장소로서, 다양한 콘텐츠를 통해 우리의 문화유산을 소개하고 있다. 이를 다중디스플레이를 활용한 문화관광 콘 텐츠로 더욱 널리 알리고 우리문화의 보존과 계승에 크게 기여한다.

Since rice is the main food in Korea, there are no regulations on corn milling yet. Corn is known as one of the world's top three food crops along with wheat and rice, and it is known that 3.5 billion people worldwide use corn for food. In addition, corn mills are not developed or sold in Korea, but the use of corn mills is increasing significantly in many countries in Southeast Asia. In the Philippines, as Korea's rice mill import increases, Korea's KAMICO (Korea Agricultural Machinery Industry Cooperative) and domestic company A agreed to develop a corn mill jointly with PHilMech, an organization affiliated with the Philippine Ministry of Agriculture. However, research on corn milling was very insignificant, so the development was carried out based on the technology of Korea's rice mill. Rice milling is performed by peeling off the skin of rice and producing brown or white rice, so it is carried out by removing the skin and cutting the skin. On the other hand, in the corn mill, the skin of the corn is peeled, pulverized and selected to produce main products suitable for edible use. Therefore, in order to develop a corn mill, processes such as peeling, transfer, grinding, sorting, and by-product separation are required, and suitable parts must be developed. In addition, the performance must be gradually improved through experiments in which corn is repeatedly milled. The Philippines produces 7.98 million tons/year of corn, which is about 100 times that of Korea, and is mostly consumed as a staple food. This is about 10% of the total crop production in the Philippines. In addition, the main cultivation complexes of corn are the mountainous regions of Tarlac or Pangasinan, and the produced corn is 72.4% of the so-called yellow corn called Arabel and Sarangani, and the remaining 27.6% are known as white corn. In this study, it was intended to produce grains of 2.5 mm or less suitable for food for yellow corn and to develop a corn mill for 200 kg per hour. Detailed conditions for development are stipulated as more than 55% of the main product recovery rate, more than 31% of the by-product recovery rate, less than 5% of the raw material loss rate, and more than 80% of the embryo dislocation rate. In this study, to achieve this, the overall process of the corn mill was developed, and the optimal conditions for the corn mill were obtained through the development of parts and empirical tests to improve performance. In addition, it was intended to achieve the development goal by evaluating and analyzing the performance of each part so that it did not conflict.

Air conditioner filters purify the air of indoor environments by removing air pollutants and supporting the efficiency of the unit’s cooling function. However, an air conditioner filter can become a microenvironment in which some fungi can grow as dust continues to accumulate and favorable humidity conditions are formed. Fungal growth in air conditioner filters could lead to fungal allergies or fungal diseases, in addition to emitting a foul odor. In an effort to understand what species causes this malodorous problem, we investigated the diversity of fungi found in air conditioners. Fungi were sampled from the collected air conditioner filters and grown on DG18 agar media. After purification for pure isolates, species identification was undertaken. Colony morphology was observed on PDA, MEA, CYA, and OA media. Microstructures of fruiting body, mycelia, and spores were examined using a light microscope. Molecular identification was performed by PCR and sequencing of PCR amplicons, and molecular phylogenetic analysis of sequenced DNA markers, including the Internal Transcribed Spacer (ITS), the 28S large subunit of the nuclear ribosomal RNA (LSU rDNA), the β-tubulin (BenA) gene, the Calmodulin (CaM) gene, and the DNA-directed RNA polymerase II subunit 2 (RPB2) gene. Through this identification process, we found two fungal species, Aspergillus miraensis and Dichotomopilus ramosissimus, which are unrecorded species in Korea. We will now report their morphological and molecular features.

Recently, the electron transport layer (ETL) has become one of the key components for high-performance perovskite solar cell (PSC). This study is motivated by the nonreproducible performance of ETL made of spin coated SnO2 applied to a PSC. We made a comparative study between tin oxide deposited by atomic layer deposition (ALD) or spin coating to be used as an ETL in N-I-P PSC. 15 nm-thick Tin oxide thin films were deposited by ALD using tetrakisdimethylanmiotin (TDMASn) and using reactant ozone at 120 °C. PSC using ALD SnO2 as ETL showed a maximum efficiency of 18.97 %, and PSC using spin coated SnO2 showed a maximum efficiency of 18.46 %. This is because the short circuit current (Jsc) of PSC using the ALD SnO2 layer was 0.75 mA/cm2 higher than that of the spin coated SnO2. This result can be attributed to the fact that the electron transfer distance from the perovskite is constant due to the thickness uniformity of ALD SnO2. Therefore ALD SnO2 is a candidate as a ETL for use in PSC vacuum deposition.