In spite of lab safety act for over 10 years, over 100 safety accidents in the laboratory have been constantly occurring. The ideal safety management system is to prevent accidents by differential classifying and managing laboratory regulatory materials according to the risk level. In order to approach this system, in-depth interviews with safety managers were first conducted to identify the current status of safety management in domestic university laboratories. And then through comparative analysis of safety management systems in domestic and foreign laboratories, a new regulatory substance classification standard based on the analysis of the hazards and the classification of risk grades, and a safety management system are proposed. From this study, it will contribute to the creation of a safe laboratory environment by differential classification and management laboratory regulatory materials based on the risk level.

Cherry leaf roll virus(CLRV)는 group IV positive sense ssRNA viruses, Nepovirus로 분류되는 식물병원성 바이러스이다. CLRV는 체리 등 목본 및 완두 등 콩과 작물을 자연 기주로 하며, 실험적으로 약 36개 과 이상의 넓은 기주 범위를 가지고 있어 국가적, 경제적 및 농가 개인적 피해를 야기 할 가능 성이 보고되고 있다. 현재 CLRV를 검출하는 방법으로 역전사(reverse transcription; RT)-nesdted 중 합효소연쇄반응(polymerase chain reaction; PCR) 이 활용되고 있으며, 다양한 기주로부터 CLRV를 검출하기 위해서는 검출 감도, 특이성, 반응 시간, 단순성 등이 중요 요소였다. 그러나 RT-nested PCR은 두 단계로 구성되어 있어 단순하지 않고, CLRV를 검출하는데 약 10시간 이상의 반응 시간이 소 요되었다. 이번 연구에서는 등온증폭법을 이용하여 단순하고 신속하게 CLRV를 검출하는 방법을 개발하 였다. 등온증폭 반응은 RT-nested PCR과 동등한 검출 감도로 CLRV를 검출 하였다. 그러나 반응 시간 을 약 2시간 수준으로 단축하였으며, 6개 영역을 사용하는 등온증폭 프라이머의 사용으로 더욱 특이적 으로 증폭 할 수 있었다.

We were conducted to know the community structure the insect fauna of the Cheonggyecheon, the urban natural restored stream. From 2007 to 2010, we were surveyed 18 times at 4 collecting sites, day and night. A total of 352 species of 99 families belonging to 11 orders were identified from 4,433 individuals collected terrestrial insects. Among them, 113 species, 52 families, 9 orders were collected in night time. Grouping by order, these 3 orders, Hemiptera (70 species in 20 families), Lepidoptera (69 species in 19 families) and Coleoptera (67 species in 17 families) were recorded high species number rather than the other orders. A yearly species number are as follows: 137 species, 57 families belonging to 10 orders were recorded in 2007; 162 species, 56 families belonging to 8 orders were recorded in 2008; 159 species, 61 families belonging to 9 orders were recorded in 2009; 166 species, 71 families belonging to 10 orders were recorded in 2010. The species number of lower stream (170 species, 53 families belonging to 8 orders from St.3 and 208 species, 67 families belonging 10 orders from St.4) was higher than that of upper stream (94 species, 47 families belonging to 8 orders from St.1 and 110 species, 48 families belonging 9 orders from St.2). The results of this study showed that diversity of the insect species in Cheonggyecheon have been keeping stable condition since 2008 after 3 years from the Cheonggyecheon restored. Accordingly, Cheonggyecheon is important habitat for terrestrial insects living in an urban environment which has severe ecological disturbances.

Oecophyllembiinae moths are small leaf mining gracillariid subfamily which is consisting of 34 species in 6 genera on the world, and their wing expanse about only 5-10mm. Since Kumata (1998) redefined subfamily Oecophyllembiinae, and Vari et al. (2002) treated Oecophyllembiinae as a synonym of Phyllocnistinae. Kawahara et al. (2011) analyzed, however, the grouping Oecophyllembiinae together with Phyllocnistinae is supported weakly or not at all, but a sister-group relationship could not be rejected. Also, because of their character distinctions on hind wing venation and the larval thoracic spiracles, we would like to follow Kumata (1998) distinguished Oecophyllembiinae from Phyllocnistinae. In thisstudy, Eumetriochroa hederae Kumata, 1998 feeding on Hedera rhombea SIEB. et ZUCC. (Araliaceae), is reported for the first time from Goheung, Korea. The host plant Hedera rhombea is a species of ivy which is native to coast of East Asia and some islands of East Asia include China, Taiwan, Japan and Korea. Larva makes irregular linear mine on upper side of leaf throughout its whole stage. The mature larva makes an oval pupal chamber within the end of the mine. The figures of the male and female genitalia, mine, pupae in immature stages are provided. The subfamily Oecophyllembiinae Real et Balachowsky (Lepidoptera: Gracillariidae) is newly recorded from Korea for the first time.

Phycitinae is one of the subfamilies of Pyralidae, about 4000 phycitinae species in the world (Solis and Mitter 1992), and around 300 species of 110 genera have been reported in China (Li and Ren 2005). The subfamily of Phycitinae is characterized by the following characteristics: both wings are long and narrow, chaetosema present; forewing with 11 veins or less, R3 and R4 stalked at base or fused completely, both male and female with only one frenulum; gnathos rod-shaped or coniform; valva with costa well developed, transtilla free or connected at top, aedeagus cylindrical, male usually with culcita; ductus bursae from corpus bursae. The larvae of phycitinae are leafrollers, leafminers or products pests. Their distribution is throughout the world (Munroe and Solis, 1999). As a NE region, Jilin province lies in the center of northeast part of China, borders by North Korea, Far East of Russia, Heilongjiang, Liaoning and Inner Mongolia. Until now, only 20 species of phycitinae have been reported from here, accordingly, the faunistic inventory is by no means completed, and to survey the diversity of phycitinae is significant for systematic study in China. In this study, we identified 35 species belonging to 19 genera, of them, 2 species are reported for the first time in China, and 12 species are newly reported from Jilin Province. The materials examined come from the collection of Incheon National University and Northeast Forestry University.

Pyralinae are one of the largest subfamilies belonging to Pyralidae, which comprises about 900 species of 217 genera in the world, and 150 species belonging to 32 genera are recorded in China (Li houhun, 2009). This subfamily are characterized by the following characteristics: head rounded, chaetosemata present, proboscis developed. Labial palpus porrect or upturned. Maxillary palpus small. Forewing colorful, from narrow to wide, antemedial line and postmedial line present and distinct. Hindwing color simple. Male genitalia with broad uncus, gnathos developed, slim, tip of gnathos sharp or hooked; tegument sclerotized, valve simple with various shape; juxta elliptical or rounded, aedeagus slender, cornutus present or absent. Female genitalia with small papillae anales, corpus bursae oval, signum present or absent. Liaoning Province is located in the southern part of NE China, the area of Liaoning is 146,900 km² and it is bounded on the south by the Yellow sea, on the southeast by North Korea, on the north by Jilin Province, and on the west by Inner Mongolia and Hebei Province. The mean annual temperature is 7-11℃, the mean annual precipitation is 600-1100 mm in northeast part, but 400 mm in west part. Liaoning Province is an important area for forest and agriculture, accordingly, to survey the diversity of Pyralinae is of great significance for systematic study in Liaoning Province. In this study, our materials examined come from the collection of Northeast forestry University from 2006-2010, and the collection of Incheon University from 2010-2012. Finally, we identified 11 species belonging to 8 genera, of them, 1 species are the first time reported in China, and 6 speices are the first time reported in Liaoning Province.

As one of the subfamilies of Crambidae, Spilomelinae comprises about 3767 species in the world (Solis and Maes, 2002), and this subfamily is characterized by the following characteristics: Chaetosema absent, antenna filiform, labial palpus and proboscis well developed, forewing with R3 and R4 stalk at base, or R2, R3 and R4 stalked, but R5 single, 2A and 1A form a loop; hindwing Sc+R1 stalked with Rs; male genitalia with gnathos absent or rudimentary, but uncus well developed, shape various. Cambodia is a country that bordered by Thailand, Laos, Vietnam and Gulf of Thailand in Southeast Asia. The total area is 181,035 km2. It is also a country with high biodiversity, of them, 212 mammal species, 536 bird species, 240 reptile species, 850 freshwater fish species, and 435 marine fish species, but only a few studies about the fauna of Lepidoptera there. Accordingly, to survey the diversity of Lepidoptera is of great significance for systematic study in Cambodia. In this research, we started our survey from 2009, up to now, we got numerous Pyraloidea from Cambodia, in this study, we identified 48 species belonging to 36 genera of Spilomelinae, most of them are reported for the first time in Cambodia, and some specimen could not be identified which we will report in future. All the materials examined come from the collection of University of Incheon.