느타리버섯 재배를 위해 알바시아나무톱밥과 기존에 사용하고 있던 포플러톱밥, 미송톱밥이 어떠한 차이점이 있는지 조사하기 위해 본 실험을 수행하였다. 톱밥재료별로혼합하여 배지의 이화학성 및 수량성을 검토한 결과 재료별 일반성분 중 C/N은 미송톱밥에서 504로 가장 높았고,T-N은 알바시아나무톱밥에서 4.03%로 가장 높았다. 페놀계화합물의 경우 알바시아나무톱바에서 21.65%로 가장높았다. 혼합배지에 따른 이화학성 중 pH는 처리구별 모두 5.1~5.2로 차이가 크게 나지 않았고, C/N범위는 처리구 모두 이와 대등한 경향을 보였으며, 그 중 T2가 20으로 가장 작았다. 혼합배지별 경시적 균사생장량을 조사한결과 T1, T3 및 T4는 모두 대등한 경향이었으나 T2의 경우 배양일수가 25일로 가장 늦었다. 혼합배지별 생육특성은 크게 차이가 나지 않았으나 유효경수는 T2를 제외한모든 처리구에서 대등하였고, T2에서 가장 작았다. 알바시아나무톱밥을 기존의 미루나무톱밥이나 미송톱밥을 전량 대체할 수는 없었으나 기존 톱밥의 50%정도를 대체할경우 수량과 회수율에서 모두 대등한 결과를 보였다

외부공기 유입방식에 따른 느타리버섯의 생육특성을 조사하기 위하여 3가지 처리구를 두어 실험한 결과 모든 처리구에서 재배기간 중 온도편차가 발생하였고, 처리구 모두 1, 2, 8월에 가장 높았으며, T2(열교환기)와 T3(기밀실)에서 T1(외부공기 직접유입) 보다 온도편차가 작은 경향이었다. 재배기간 중 냉방시간은 T1(외부공기 직접유입)은 1,090.3시간, T2(열교환기)는 661.1시간, T3(기밀실)은 865.0시간이었고,평균 냉방절감율은 T1(외부공기 직접유입) 대비 T2(열교환기)는 21%, T3(기밀실)은 11%이었다. 재배기간 동안 난방기의 년중 가동시간은 T1(외부공기 직접유입)은 1,506.9시간, T2(열교환기)는 875.6시간,T3(기밀실)은 1,054.2시간으로 T2의 가동시간이 가장 작았고, 년중 평균 난방절감율은 T1(외부공기 직접유입) 대비 T2(열교환기)는 22%, T3(기밀실)은16%이었다. 외부공기 유입방식에 따른 냉방정도(CD-H)는 T1(외부공기 직접유입)>T3(기밀실)>T2(열교환기)의 순이었고, 난방정도(HD-H)도 냉방정도(CD-H)의 경우와 동일하였다. 수량은 T2(열교환기)와 T3(기밀실)에서 T1(외부공기 직접유입) 보다 4,9, 10월을 제외한 전 기간에서 높았다.이와 같은 결과로 시설재배사에서 느타리버섯 병재배시 열교환기 또는 기밀실을 설치하여 재배할 경우에너지를 절감할 수 있고, 수량 및 고품질의 느타리버섯을 수확할 수 있어 농가소득증대에 기여할 것으로 판단되었다.

잣버섯 생육환경조건 중 생육온도를 설정하기 위하여 실험한 결과 생육온도 20℃에서 상품수량이 가장 우수한 결과를 나타 내었으며 다음과 같다. 생육온도에 따른 갓의 명도값은 차이가 나지 않았으나 적색도 및 황색도는 온도가 높을수록 높아지는 경향이었고, 대의 경도 및 깨짐성은 17 및 20℃에서 높았다. 잣버섯의 생육온도에 따른 자실체의 생육특성은 온도가 높을수록 발이유도기 및 자실체의 발생기간이 빨랐으나 수량은 모두 유의성이 나타나지 않았다. 그러나 상품수량의 경우 20에서 가장 높았다. 갓크기는 생육온도 20˜23℃에서 17℃ 보다 컸고, 대굵기는 17℃에서 가장 굵었다.

잣버섯 재배를 위해 도입육성된 솔향의 적정 균사생육 온도는 26~32℃이었다. 재배적 특성에 있어서 배양일수는 30일, 초발이소요일수 7일, 생육일수는 6일로 총 재배일수가 43일이었으며, 갓색은 연노랑색이었다. 미송톱밥+옥분(90:10)의 배지에 봉지재배로 3차에 걸친 생산력 검정시험을 수행한 결과 수량은 115g이었으며, DNA 다형성 검정결과 GMLL66035와는 다른 국내 고유의 자생균주로 확인되었다.

We aim to elucidate suitable color of light during development of fruit body in Pleurotus eryngii. The four color of LED(Light Emitting Diode), blue LED(475nm), green LED(525nm), yellowed LED(590nm), and red LED(660nm), were irradiated for formation of fruit body after mycelia growth. First, as effect of color of LED at all growth stage, the morphological properties of fruit body in fluorescent lamp and blue LED irradiation were showed thicker and longer pileus than those in other color LED irradiation. The commercial yields in blue and green LED treatment were similar to control(Fluorescent lamp). Second, as effect of color of LED by light irradiation time, we could obtain the highest commercial yields at the green LED continuous radiation, 1/1 and 1/2(lighting/lighting-out, hours). And we could obtain the highest number of available stipes and biological efficiency at the 1/1(lighting/lighting-out, hours). The ergosterol was the highest under the green LED continuous radiation.

We found about cultivation environment conditions of N. lepideus. For the high quality of N. lepideus, lights-out was useful. In the lights-out, the diameter of pileus of N. lepideus was smaller, and the length and the thickness of stipes were longer and larger than in that of lighting. For the efficient cultivation of N. lepideus, temperature of mycelial growth and development of the fruit body were suitable 20～23℃ and 20℃, respectively. In addition, we found higher yield and better quality of N. lepideus at 1,500～2,000ppm of CO2 concentration during the development of fruit body.

This study was carried to investigate the suitable conditions of Pleurotus eryngii through precooling for the more long-term freshness. The time to be same temperatures between P. eryngii and storage room through precooling at 0℃ and 4℃ were showed 2 hours and 5.5 hours, respectively. P. eryngii was cooled within 6 hours and 18 hours at 0℃ and 4℃ with two type, forced air cooling and pressure cooling. After precooling, P. eryngii was packed 400±10g with anti-fog film, and then stored at 4±1℃. In all treatment of precooling, the weight loss was peaked at 25 days and hardness of P. eryngii were decreased at 10 days during storage. There were no significant freshness differences between precooling type and conditions. It was found that the optimum stored period of P. eryngii at 4℃ after precooling was estimated to be 30~35days. Consequently, it is necessary to elucidate efficient and economic precooling conditions.

생육온도가 증가함에 따라 CO2(ppm/hr/㎥/병)농도는 증가하였고, 생육단계별로는 생육후기에 가장 높은 경향을 나타내고 있으며, 품종별로 춘추2호<수한2호<청풍<진미<흑백 순으로 호흡량이 많은 경향을 나타내었다. 또한 생육온도가 높아짐에 따라 O2(ppm/hr/㎥/병)소비량은 증가하는 경향이었으며, 생육단계별로 생육후기에 가장 많이 소비되는 경향을 보이고 있고, 품종별로 춘추2호<수한2호<청풍<진미<흑백 순으로 소비된 O2량이 많아지는 경향이었다. 그리고 느타리버섯의 생육적정 온도인 15℃에서의 호흡지수는 춘추2호가 500일 때, 수한2호 528, 청풍 573, 진미 617, 흑백 634로 신품종의 호흡지수는 춘추2호 보다 높은 경향을 나타내었다.

Lentinula edodes mushrooms cultivated under different relative humidities were wrapped at 4℃ and the results of storage characteristics were investigated. Changes in water content of fruiting bodies during the storage period showed the highest water content in fruit bodies harvested from the treatment with the highest relative humidity. The luminosity of the fresh fruiting bodies showed no significant change during the storage period, and the redness was higher in the relative humidity 95% treatment than in the other treatments. According to this study, the relative humidity of the pileus was 65%, and the content of Ergosterol was 0.67 ± 15 g / L at relative humidity of 65%, 80% and 95%. In addition, amino acid analysis and Principal Component Analysis (PCA) confirmed that methionine was the main cause of changes in storage time and relative humidity.

This study analyzed the quality characteristics of black garlic made from Seosan Yukjok Garlic and elephant garlic in Seosan, Chungnam province. Of the inorganic components, Mg content was the highest in all treatment groups, and the Ca content was high in each of the 15 day treatments. The content of K was high after 10 days aging in Yukjok garlic and after 15 days in the elephant garlic. The Fe, Na, K, and Mg content was high in Yukjok black garlic after 15 days, and Na, K, Ca, and Mg were high in the elephant black garlic aged for 15 days. The crude fat content was high in both Yukjok black garlic and elephant black garlic after 15 days. Vitamin C content was highest in both types of garlic after aging for 15 days. An analysis of four kinds of organic acids showed that citric acid was the only organic acid to appear in raw garlic of Yukjok garlic and elephant garlic. Black Yukjok garlic and elephant black garlic had a greater total amino acid content than the raw garlic of either type. However, among the tested amino acids, 13 kinds of amino acids were at their highest after five days of ripening in Yukjok black garlic, while 15 kinds of amino acids were abundant in elephant garlic after the same period. Eight kinds of amino acids were high after aging for 15 days. Through this study, it was confirmed that, in the process of making black garlic, changes in the main components of the garlic occur through different routes, and these changes vary depending on the garlic species. Therefore, this study provided basic data for the processing of Seosan's Yukjok black garlic and elephant black garlic.

The purpose of this study was to investigate eco-friendly measures to manage major diseases which cause heavy economic damages to ginseng. Morphological, physicochemical, and molecular biological species identification was carried out after isolating useful antagonistic bacteria from ginseng fields. In addition, optimal conditions for mass culture were established, and he efficacy of the bacteria in the prevention of the diseases was verified in the field. The results showed that about 150 bacteria were extracted from 150 ginseng fields in the whole county. Among them, B. pyrrocinia LA101 was finally selected, which had a strong antagonistic potency against Alternaria panax, Botrytis cinerea, Rhizoctonia solani, and Cylindrocarpon destructans on agar media. The B. pyrrocinia LA101 is a baculiform gram-negative bacterium identified as Burkholderia pyrrocinia according to results from an API(Analytical Profile Index) kit, 16S rRNA, and gyrase gene sequencing analysis. It was donated to the microbe bank of the Agricultural Genetic Resources Center at the National Academy of Agriculture Science under the Rural Development Administration on September 28, 2011 (Donation No. KACC91663P). A patent for the mass culture technology was granted in August 2012 (Patent No. 10-1175532).

This study was carried out to investigate the changes in the amount of 20 free amino acids in PDB (Potato Dextrose Broth) medium during the incubation period while cultivating Lentinula edodes. The total incubation period was 90 days, and the total amount of free amino acids was confirmed every 45 days. Among the 18 kinds of amino acids whose increase and decrease patterns were confirmed, 10 amino acids were increased compared to that in the control, among which cysteine increased from its initial value of 9,889 ± 3 μg/L to 12,909 ± 2 μg/L at 45 days and 29,256 ± 4 μg/L at 90 days. Six amino acids with decreased expression patterns were identified. Arginine decreased to 83,751 ± 2 μg/L after 45 days from its initial value of 161,787 ± 1 μg/L and to 79,055 ± 7 μg/L at 90 days.

This study investigated the relationship among seven species of trichoderma through the identification of strains collected in Korea. The phylogenetic tree among the collected strains was classified into four groups. The trichoderma strains isolated in this way showed inhibitory effect on the fusarium wilt which is parasitic to cotyledon stem..The invisibility of J9, J10, J13 and J16 strains were higher in comparison with other strains in vitro test stand, and their spore production level was also higher. In the aluminum ring tests, it showed that the yield of the spores in J9, J10 and J13 were more than any other strain. As a result conducting the port test for cucumbers, the plant lengths of J13 were larger than the control plot, and the root lengths of all strains, except for J2 were longer than the control plot, and the root weights of J1, J9, J10, J13 and J16 were larger than the control plot. The disease severity for the fusarium wilt showed the smallest values at J13 and J16 in comparison with the control plot, and the control values of J13 and J16 were higher than other strains.

This experiment was conducted to assess changes to the chemical properties of soil and applicability in a case of rice cultivation with organic fertilizers. The investigator applied organic fertilizers to rice cultivation for five years to examine changes to the chemical properties of soil and found that the experiment group of organic fertilizers made an ongoing increase in pH, organism content, and available phosphate content annually compared with the control group with no big differences according to the amounts of organic fertilizers used. As for the yield components, there were no statistical differences in the number of spikelets and grain filling rate between the experiment group of organic fertilizers and the control group. The experiment group recorded a higher level in 1,000 seeds weight and yield than the control group. Experiment Group 4 recorded the highest level at 29.11 kg of 1,000 seeds weight. Experiment Groups 3 and 4, which used 222 kg and 267 kg per 10 a, respectively, recorded 576 kg and 572 kg of yield, respectively, which were 4.7% and 4.1% higher than 549 kg of control group, respectively. As for the quality of brown rice, there were no statistical differences in the head rice percentage between the control and experiment group, both of which were in the range of 83.2-85.7%. As for the protein content, Experiment Groups 3 and 4, both of which used a lot of organic fertilizers, were in the range of 6.9-7.1%, which was lower than 7.5% of control group. Those findings indicate that the long-term application of organic fertilizers can improve the chemical properties of soil and increase the yield more than the conventional method of fertilizer application. The findings also suggest that it will be effective to apply 222 kg of organic fertilizers or more per 10 a.

The purpose of this study was to develop the best mushroom cultivation conditions and the combination of mushroom culture media in order for mushroom producers and consumers. To reach this target, we first investigated the genetic relationship and developed suitable conditions of mycelial growth in Hypsizygus marmoreus strains. One superior strain of H. marmoreus was selected from 124 strains using bag culture. One hundred and twenty four strains were genetically classified into four main groups using two Universal Rice primers, URP2R and URP17R. The studies on the effects of different temperature (17, 21, 25, 29, 33℃) showed that 25℃ is the best temperature for mycelial growth for almost all strains while at 33℃ most of mycelium stop growth. Finally, ten strains were selected according to the groups identified by their temperature requirements. The length of mycelial growth in PDA, MCM, GPYM, MEA and MYP were longer than those in Czapek Dox. The selected ten strains of H. marmoreus showed heavier dry weight of mycelia at pH 3.0∼7.0 than any other pH. Although it was not show distinct requirement of carbon and nitrogen sources for vegetative growth according to strains, mainly the mycelial growth of the ten selected strains were observed at media including xylan and yeast extract, peptone, tryptone, respectively. Moreover, higher C/N ratio was observed in higher dry weight of mycelia.