The effect of the ionization energy on the common house mosquito, Culex pipiens, pupa was investigated as the hatching rate, longevity and detoxification enzyme activity. The longevity of adult emerging in the pupa decreased depending on the exposure dose of Gray (Gy) in Cx. pipiens. The hatching rate of the F1 generation egg derived from Cx. pipiens pupa treated up to 70 Gy did not show any difference with the control, but the eggs did not hatch 100% at 120 Gy exposure. These results showed that Cx. pipiens was sterilized at 120 Gy. Several detoxifying enzyme (GST, non-specific esterase and MFO) activity of Cx. pipiens adult emerged from pupa irradiated with 120 Gy was investigated. The difference in activity was observed in female than in male irradiated with electron beam. As a result, electron beam irradiation seems to affect enzyme activity as well as induce infertility of Cx. pipiens.

5개 지역 양계장에서 채집한 닭진드기(Dermanyssus gallinae) 성충에 대한 11종 살비제의 감수성을 조사하였다. Bifenthrin과 formic acid + pyridaben은 100배의 희석배수로 처리한 결과, bifenthrin은 5가지 지역에서 모두 100%의 살비율을 보였으나 formic acid + pyridaben 합제 는 연천과 안성을 제외한 나머지 3지역(경주, 칠곡, 금산)에서 20% 이하의 낮은 살비율을 보였다. 카바메이트계 약제인 carbaryl은 경주(45.0%) 를 제외한 4지역에서 100%의 살비효과를 보였다. 유기인계 약제인 dichlorvos는 연천을 제외한 4지역 모두 100%의 살비효과를 보였지만 연천 은 66.7%의 살비율을 보였다. 연천과 안성지역의 양계장이 살비제에 대한 감수성이 높게 나타났으나 다른 3지역(경주, 칠곡, 금산)의 경우에는 약제에 따라 활성의 차이를 보였다. Clothianidin, thiamethoxam, fenitrothion, formic acid + pyridaben은 지역에 따라 살비활성의 효과에 차이를 보여 사용 시 주의를 기울여야 할 것이나, carbaryl과 cartap hydrochloride, dichlorvos, bifenthrin은 실험에 사용된 모든 지역에서 활 성이 높아 모든 지역에서 사용가능할 것으로 생각된다. 양계장별 살비제 활성이 차이를 보이는바 효과적인 닭진드기의 방제를 위해서는 살비제의 활성평가와 더불어 약제의 교호살포와 무분별한 사용을 지양해야할 것으로 보이며 본 연구가 닭진드기 방제를 위한 기초 자료로 활용될 수 있을 것으로 생각된다.

Cooked rice stickiness is one of the critical quality factors. A new data processing method from a simple experiment detaching after compressing cooked rice on a platform of texture analyzer was developed to create a new instrumental parameter which represents a true stickiness from sensory evaluation. The proposed idea was that the contact area produced by compressing with a fixed magnitude of force should vary with the sample’s consistency, ending up distorting the stickiness simply measured as the detaching force. Therefore, a corrected stickiness was defined by considering the contact area and converting to a stress (force/area) from just a force. The contact area could be estimated from the volume, density and thickness of samples. Even the compression force was converted to a corrected compression force by dividing by the contact area. The new corrected parameters turned out well to show a much better agreement with the sensorial stickiness than the uncorrected stickiness.

Since a free-learning semester has been introduced to middle school curriculum, interest in career experience programs has increased. To respond to this new demand, we developed a continuous agriculture-related career experience program linked with STEAM (Science, Technology, Engineering, Arts, and Mathematics) education, and we examined the program’s effects on the job awareness of middle school students. The program was conducted from April 8, 2015 to June 24, 2015, and consisted of 12 classes. The direct experience subprogram was based on 13 different horticultural activities that reflect producing (nine programs), cooking (two programs), and sales (two programs) of horticultural produces. For the indirect experience subprogram, we preliminarily selected 10 representative agricultural jobs, and based on the keywords analyzed from 7 direct experience programs, we chose to introduce seven jobs to the students by videos and the three others by written material. We then investigated the effect of introductory videos on job interest, understanding, and preference by the students. We found that the student’s interest, understanding, and preference regarding each job were significantly improved when direct experience and video watching were combined. Career awareness was improved for gardener (47.0%), nursery grower (42.0%), rural extension official (31.1%), eco-friendly farm material developer (30.9%), agricultural research official (23.4%), plant doctor (19.8%), and agricultural wholesale distribution experts (9.3%). Conclusively, it is expected that career experience programs have the greatest effect on students when both interest in the job and participation on direct experiences are encouraged.

Zika virus, mosquito-borne disease, caused by mosquitoes has been increased the importance. From March to September, twice a month from 7 different points (3 residential areas, 3 migratory bird sanctuary and 1 cattle shed) were collected using BG trap and BL trap. After identifying the mosquitoes collected, we confirmed the virus infection. Total 26,531 mosquitoes (6 genus 9 species) were collected, virus has been detected from the 3 species (Aedes vexans, Cuilex tritaeniorhynchus, and Amigeres subalbatus) of mosquitoes of them. It showed the highest peak in August, and then gradually decreased. The most common mosquito species was collected Aedes vexan (16,637) in the cattle shed.

A self-powered time-temperature indicator (TTI) was optimized to enhance the performance of the TTI by modifying a biofuel cell using different immobilization, redox mediators, and status of electrode. The performance of the TTI was measured by output voltage of the TTI. The enzymes and combinations of lacasse mediators (HBT (1-hydroxybenzotriazole), Rupy (Bis-(bipyridine)-(5-aminophenanthroline) ruthenium bis (hexafluorophosphate)), MB (Methylene blue), SDP (4,4- sulfonyldiphenol)) and glucose oxidase mediators (FA (Ferroceneboxaldehyde), DBQ(2,5-dihydroxybenzoquinone), HQS (8-hydroxyquinoline-5sulfonic acid hydrate), FHFP (Ferrocenium hexafluorophosphate)) were immobilized with stabilizers (pyrrole) on a glassy carbon electrode by electrodeposition by applying a square wave, cross-linking and physical immobilization. MWCNTs were used to modify glassy carbon electrodes. MWCNTs coated electrodes produced higher output voltage than uncoated electrodes. The optimum and stable performance of the self-powered TTI was that the output voltage of 64 mV and duration time was 3hr at 25°C, when the combination of Rupy, MB for laccase mediators and HQS, FHFP for glucose oxidase mediators were immobilized on MWCNTs coated electrodes by applying a square wave method. In the application, the concentrations of enzyme and glucose were adjusted to prolong the shelf-life of TTI at much lower output voltage.

The pasteurization is employed for extending shelf-life and keeping the quality of products constant. However the pasteurization undesirably accelerates the oxidation of beer which renders volatile compounds concerning off-flavor. The pasteurization conditions was optimized to avoid off-flavor of beer during pasteurization. Under the isothermal condition, thermal destruction kinetics such as D and Z value of Lactobacillus brevis which is reported the most common beer spoilage, was determined. The binomial data (detected or non-detected of off-flavor) was treated with logistic regression to estimate off-flavor development (OFD) times. The temperature dependence of OFD times was established in terms of Arrhenius relationships. Optimized pasteurization temperature and time were found at which OFD times was not detected. The constraint for optimization was that the pasteurization degree should be larger than 5 decimal reduction time. The optimization was conducted through mathematical simulation using kinetics and temperature-dependent models for microbial death and OFD times. The optimized results were validated by the corresponding experimentations, which met the requirements that the concentration of Lactobacillus brevis was 5-Log reduction and the OFD times not detected.

Makgeolli is rich in nutrients such as beneficial microbial species, essential amino acids, oligosaccharides, and organic acids. The lactic acid bacteria contained in Makgeolli provides a healthy function as probiotics for customers. The purpose of this study was to enhance the nutritional properties, lactic acid bacteria propagation and alcohol contents of makgeolli by controlling redox potential balance. An automatic control system was created using ORP sensor, Labview control kit, and air supplier, in which air concentration was controlled by on & off mode. Makgeolli was fermented at three different redox potentials -50 mV, -100 mV, -150 mV. Regulating of aeration according to the redox potentials could give prominence to nutritional benefits of Makgeolli. The profiles of redox potential appeared bathtub curves, and related to lactic acid bacteria growth curve and byproducts. We could find the optimum redox potential balance that affects the factors such as LAB’s, alcohol contents and byproducts. In conclusion it was essential to control redox potential balance in order to produce nutrients rich Makgeolli.

A novel nanocomposite LDPE film with UV protective properties was developed for active packaging applications. Initially, undoped and Mn-doped TiO2 nanoparticles (NPs) were synthesized by the sol-gel method and the resulting particles were characterized. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images revealed an agglomerated nature and spherical morphology. X-ray diffraction (XRD) studies indicated that all products were crystalline and in the form of rutile. The reflectance spectrum of undoped TiO2 NPs demonstrated a characteristic sharp edge at 410 nm. Subsequently, nanocomposite (NC) LDPE samples were prepared with the NPs by solvent precipitation followed by film casting. The optical and thermal properties of the NC samples were investigated. Incremental increases in Mn concentration from 0.25 mol % to 1.00 mol % were associated with progressive decreases in light transmission in the UV region. The melting and maximum decomposition temperatures of all NCs were 107 and 442-449 °C, respectively. The UV protective LDPE-based NC films exhibited superior photostability. Absorption in the FTIR spectra at 1716 and 1734 cm-1 changed after 4-wk exposure to UV for all film samples as a consequence of photodegradation. Finally, the photooxidation of perilla oil was assessed as an example of a UV protective packaging application. After 12 days, protection with 1.00 mol% Mn-doped TiO2-LDPE was associated with a gradual increase in PV, while protection with TiO2-LDPE was associated with a significant increase and protection with the control treatment was associated with a dramatic increase in PV. Hence, a 1.00 mol% Mn-doped TiO2-LDPE NC showed promise for UV shielding packaging applications.

Time-temperature indicators or integrators (TTIs) indicate food quality changes based on time-temperature history. Whilst many types of TTIs have been developed and commercialized, educated consumers often refuse to purchase food products with attached TTI labels showing even a slight color change. In this study, a novel on-off diffusion-based TTI coupled with polydiacetylene/silica nanocomposites has been proposed. The prototype TTI tag has a multilayer structure comprised of a self-adhesive base layer, a middle microporous sheet, and an upper opaque white layer coupled with a square reservoir of Tween 20 attached to an activation stripe. At the end of the diffusion path, polydiacetylene/silica nanocomposites were injected into a loading site as a fine blue stripe. After activation, Tween 20 diffused and reached the loading site, where it rapidly changed from blue-to-red via solvatochromism. This alternative and innovative TTI continuously showed a blue color until reaching the end point, at which stage a red color rapidly appeared, indicating product rejection. Thus, this novel TTI it is of great benefit to the brand owner. The developed prototype was characterized and evaluated for its ability to monitor microbial quality based on published, isothermal, microbial growth data of modified-atmosphere packaged minced beef, Mediterranean fish, and ground pork. The diffusion of Tween 20 in the TTI system was measured under various isothermal conditions and a kinetic model, based on the association between diffusion and time-temperature, was investigated. The Gaussian-estimated activation energy value was 51.082kJ mol-1. Tween 20 diffusion of 6.10, 5.15 and 6.15mm along the TTI systems were considered to be end points and the 95% confidence interval between the times taken for TTI to display OFF and for the foods to reach their deterioration thresholds were 23.30-23.70, 23.00-23.50 and 23.44-24.05h for total aerobic bacteria, Shewanella putrefaciens, and Pseudomonas spp. respectively. The TTI performance test for reproducibility and accuracy revealed a normal frequency distribution with 35004.90, 1200.254.82 and 549.811.09min at 0, 11 and 25C, respectively in accordance with the investigation of diffusion in the TTI.

In this study, the effectiveness of electrodialysis in removing inorganic arsenic from groundwater was investigated. To evaluate the feasibility of the electrodialysis, operating parameters such as treatment time, feed concentration, applied voltage and superficial velocity were experimentally investigated on arsenic removal. The higher conductivity removal and arsenic removal efficiency were obtained by increasing applied voltages and operation time. An increase of salinity concentrations in arsenic polluted groundwater exerted no effects on the arsenic separation ratios. Arsenic polluted waters were successfully treated with stack voltages of 1.8 ~ 2.4 V/cell-pair to approximately 93.4% of arsenic removal. Increase flow rate in diluate cell gave positive effect to removal rate. However, increase of superficial velocity in the concentrated cell exerted no effects on either the conductivity reduction or on the separation efficiency. Hopefully, this paper will provide direction in selecting appropriate operating conditions of electrodialysis for arsenic removal.

This study was conducted to investigate the effectof supplemental lighting intensity (SLI) on the shoot growthand flower quality of Rosa hybrida ‘Pink Bell’ in winter sea-son. High pressure sodium lamps (HPS) which were set upat 1.4m height above the planting beds were adjusted forthe SLI treatments: no supplemental lighting (control), pho-tosynthetically active radiation (PAR) 30, 50, 70, and 90µmol•m−2•s−1 in 20h daylength (17:00 to 22:00 and 02:00to 09:00). Shoot growth including shoot elongation, shootweight, and flowering speed was promoted quantitatively asSLI increased. But the flower quality such as petal num-bers, petal pigmentation, and biomass distribution to thepetals was declined at PAR 90, even though the proper SLIfor rose production was generally known in the range ofPAR 90 to 120. In this experiment PAR 90 treatment madethe surface of petals heated near 30oC which was about10oC higher than the control group without supplementallighting. The surface temperature of the petals was continu-ously rising because the distance to lamps from floral budsbecame increasingly short as the shoots grew in localgreenhouse facilities. The heat stress by HPS lampscaused petal discoloration and over-consumption of assimi-lation products due to excessive respiration and water lossin petals. Thus, it is necessary to prevent heat injury byswitching light intensity depending on shoot growth consid-ering local greenhouse structure.

The effects of ultrasound and enzyme treatments on reducing sugar production from Korean 6-row barley Dahyang during the mashing process of brewing were studied. The concentration of reducing sugar produced by the traditional method was 41.5 mg/mL. The treatment of both the ultrasound at 400 W and amyloglucosidase (0.1 U/mL) increased the reducing sugar concentration produced in the mashing process by 15% compared to the traditional method. In addition, both treatments reduced the total mashing time from 115 min to 70 min. The combination of ultrasound and amyloglucosidase treatment increased the reducing sugar production yield by 17% during mashing, in comparison with the traditional method.

Urbanization is one of the leading causes of habitat loss, habitat degradation, and fragmentation. Urban development negatively affects biodiversity. This study aimed to clarify the change of butterfly communities on effect of urbanization in urban green areas. Butterfly survey was conducted using the line transect methods from April to October in 2012. A total of 59 species and 1,465 individuals of butterflies were observed in four urban green areas: Namsan Park (NS), Ewha Womans University (EW), Bukseoul Dream Forest (BD), and Hongneung Forest (HF), and natural forest: Gwangneung Forest (GF). The category of land use around study site was determined based on GIS data. Species richness and abundance of niche breadth and habitat type in urban green areas differed significantly from those in GF. Estimated species richness and species diversity (H’) in four urban green areas were significantly lower than those in GF. Species richness and abundance of forest interior species and specialist were positively correlated with paddy, field, and forest, whereas those of forest interior species and specialist were negatively correlated with urban area and road. Butterfly communities in four urban green area differed from that in GF. The result suggests that the decrease of paddy, field, and forest associated with increase of urban area and road negatively influences species composition and changes butterfly communities.

본 연구는 6조 보리를 이용하여 양조한 맥주의 발효 중여러 특성의 변화를 측정하여 6조 보리 맥주의 제품 가능성을 확인해 보았다. 실험은 2조 보리 1종과 6조 보리4종을 이용하여 제맥, 당화, 발효 공정을 통해 맥주를 제조하였으며, 제조 공정 중 발효 특성의 변화를 확인하였다. 발효 특성 항목으로 효모의 생육, 비중, 색도, 거품 안정성,free amino acid, 13개의 관능 속성 변화를 측정하였고, 최종 제품인 맥주의 알코올 함량, 쓴맛, 탁도, diacetyl을 측정하였다. 결과적으로 6조 보리인 다향이 2조 보리인 진양과 발효 kinetics 특성이 가장 유사하게 나타나 맥주 제조에 적합한 것으로 나타났다.

Food web of main arthorpods in 3 differently managed rice fields (low input, duck, apple snail farming) was investigated in Hongseong area, Chungcheongnam-do, Korea. Main arthropods were selected by their abundance during the rice growing season in 2010. Food web was analyzed with stable isotope, δ15N and δ13C according to rice growth satges (seedling, tillering, booting, blooming/heading, and ripening stage). Rates of δ15N and δ13C in soil (F=5.72; df=2, 6; P=0.0407, F=40.86; df=2, 6; P=0.0003, respectively) and δ13C in rice (F=6.46; df=2, 6; P=0.0319) were statistically different. Rate of δ13C in Lissorhoptrus oryzophilus (F=9.50; df=2, 6; P=0.0138), Pirata subpiraticus (F=11.00; df=2, 6; P=0.0098), and Pachygnatha clercki (F=6.33; df=2, 6; P=0.0333) were statistically different among farming methods. Stable isotope structures in food web were slightly different among 3 farming methods seasonally. Rates of δ15N and δ13C of rice pests such as Nephotettix cincticeps, Nilaparvata lugens, Laodelphax striatellus, and Sogatella furcifera were related to rice plant. Those of Tomoceridae sp. and Chironomidae sp., however, were not related to rice plant. Spiders as main natural enemy group were ranked on the top of food web and were thought to have life cycle based on the rice field from stable isotope structure.

본 연구는 쌀을 다량 첨가하여 양조한 맥주의 품질개선을 위하여 아밀라아제, 프로테아제, 쌀에 결핍된 아미노산의 첨가를 시도하여 그 품질 개선의 효과를 분석하였다. 품질 요소는 당화액인 wort의 당도, 환원당, pH, 점도의 변화를 측정하였고, 최종 제품인 맥주의 알코올 함량, 거품 안정성, 쓴맛, 색도의 변화를 측정하였다. 실험에는 200 mesh의 쌀가루를 맥아와 쌀가루의 총량에 대하여 0, 40, 50, 60, 70%를 첨가하였다. 실험 결과 쌀 함량이 증가함에 따라 wort의 당도, 환원당 및 점도는 감소하였고, pH는 증가하여 당화력이 떨어졌다. 그러나 아밀라아제 첨가에 의하여 당화력이 다시 회복되었기 때문에 아밀라아제 첨가로 쌀맥주 제조 및 제품의 성능이 향상되는 결과를 보였다. 쌀 함량 증가에 따라 맥주의 알코올 함량 및 거품 안정성은 감소하였고, 쓴맛과 노란색이 증가하였다. 그러나 프로테아제 첨가에 의하여 알코올 함량, 쓴맛 및 노란색은 다시 회복되었으나 거품 안정성은 더욱 감소하였다. 글루타민산의 첨가에 의하여 노란색 외의 성질은 모두 회복되었다. 그러나 라이신 및 프롤린의 첨가에 의하여 알코올 함량 및 쓴맛은 회복되었으나 거품 안전성이 낮아지고, 색이 비정상적으로 탁하게 나타났다. 결과적으로 쌀 첨가 맥주의 품질향상 인자는 아밀라아제의 첨가, 글루타민산의 첨가, 프로테아제의 첨가의 순으로 결정되었다.

본 연구는 쌀을 다량 첨가하여 양조한 맥주의 품질을 조사하고 쌀의 첨가 시 당화도가 떨어지는 것을 보완하기 위하여 아밀라아제를 첨가하여 품질의 변화를 분석하였다. 품질 평가를 위하여 당화액인 wort의 당도, 환원당, pH, 점도의 변화를 측정하였고, 최종 제품인 맥주의 알코올 함량, 거품안정성, 쓴맛, 색도의 변화를 측정하였다. 실험에는 쌀을 200 mesh의 쌀가루를 맥아와 쌀가루의 총량에 대하여 0, 40, 50, 60, 70%를 첨가하였다. 실험결과 쌀 함량이 증가함에 따라 wort의 당도, 환원당 및 점도는 감소하였고, pH는 증가하여 당화력이 떨어졌다. 그러나 아밀라아제 첨가에 의하여 당화력이 다시 회복되었다. 쌀 함량 증가에 따라 맥주의 알코올 함량 및 거품안정성은 감소하였고, 쓴맛과 노란색이 증가하였다. 또한 쌀 첨가량이 증가할수록 혼탁한 느낌이 강하였다.

혼합물실험계획법(mixture experimental design)을 적용하여 라면 밀가루 혼합비를 최적화하였다. 밀가루 혼합비를 최적화하기 위하여 전체 기호도(overall palatability)의 최대화, 반죽 레올로지 특성의 특정 범위 유지를 최적화의 목적으로 설정하였다. 라면은 가장 보편적인 미국산 밀가루 중 강력분인 DNS, 중력분인 HRW, 박력분인 SW를 simplex-lattice 방식에 의해 혼합비를 달리하여 제조하였다. 각 시료는 Rapid Visco Analyser(RVA), 파리노그래프, 익스텐소그래프를 이용하여 반죽의 레올로지 특성치를 측정하였으며, 조리된 라면의 전체 기호도를 관능 검사 하였다. 정준상관분석(canonical correlation analysis)를 통하여 RVA의 최고점도(PV), 파리노그래프의 반죽형성시간(DT), 익스텐소그래프의 R/E 45 min을 주요 반죽 레올로지 특성치로 선발하였다. 최적화 목적으로 전체 기호도의 최대화와 최고점도(PV)(최대화), 반죽형성시간(DT)(최소화) 및 R/E 45 min(최대화)를 지정하였다. 그 결과 최적화된 밀가루 혼합비는 DNS 33.3%, HRW 33.3% 및 SW 33.3% 이였으며, 이때 전체 기호도는 5.825, 최고점도(PV)는 587.9 cP, 반죽형성시간(DT)은 3.1 min, R/E 45 min는 2.339 BU/mm로 나타났다.

중고압 하에서 β-glucosidase 효소반응을 물리화학적 관점에서 연구하였다. 모델 기질 (p-nitrophenyl-β-D-glucopyranoside)에 대한 β-glucosidase 효소의 작용에 대한 압력 효과를 실험하였다. 즉, 압력 조건(25MPa, 50 MPa, 75 MPa, 100 MPa)과 시간 (10분, 60분, 1시간, 6시간, 24시간, 40시간)의 처리 조건에서 효소 활성도를 분광학적인 표준방법에 따라 측정하였다. 효소-기질 반응의 단계를 크게 kinetic 구간과 평형 구간으로 구분하여 물리화학적 모델을 적용하여, 정역반응속도 상수, 평형상수, 압력에 의한 부피 감소 등을 산출하였다. 대기압에서 100MPa까지 압력이 증가할수록 효소-기질 반응의 생성물이 더 많이 형성되었으며 전형적인 kinetic 구간과 평형 구간이 나타났다. 압력, 시간, 생성물농도 등의 데이터로부터 kinetic 구간과 평형에서의 생성물 예측 모델을 완성하였다. 결론적으로 중고압 처리에 의하여 효소-기질 반응이 촉진됨을 알 수 있었고, 임의의 압력 및 시간 조건에 따른 생성물의 농도를 예측할 수 있게 되었다.