Extracts from Aloe vera leaves, Aloe arborescens leaves, Aloe vera callus, Portulaca oleracea and cacao (Theobroma cacao L.) bean husk (CBH) were prepared using acetone, chloroform, ethanol, hexane, and water. Solvent extracts of Aloe vera leaf had very high antioxidant activities showing IC50 values in the ranges of 0.02-0.17 mg/ mL, and had the highest total phenolic and flavonoid content among the tested samples. We hypothesized that Aloe vera leaf and CBH extracts might possess considerable in vitro anti-glycation activities. Indeed, these extracts strongly inhibited the formation of advanced glycation end-products from RNase in the presence of ribose. The chloroform extract of Aloe vera leaf showed the strongest inhibition of AGE formation (99.9%), followed by the 95% acetone extract (92.8%) at a concentration of 1 mg/mL, exhibiting higher anti-glycation activities than those of AG and rutin (73.4% and 96.1% at 1 mg/mL, respectively). The anti-glycation activity of all extracts was correlated positively with their total contents of phenolics and flavonoids. We conclude that Aloe vera leaf extracts and their constituents may be used as anti-glycation agents.

The glucomannan content of Aloe vera gel was measured by a novel method using a bentonite suspension that floc-culates upon mixing with gels prepared by hand filleting of aloe leaf. An optimum flocculation condition was deter-mined to be Aloe vera gel diluted in the range of 2 to 5-fold and then a bentonite suspension 1% (w/v) mixed withthe gel sample in the ratio of 8:2 (v/v) to provide a mixture volume of 10mL with the length to diameter ratio of5. Under the conditions of these experiments, a rapid settling (<3 min) of more than 90% of the bentonite wasachieved only when the aloe gel sample was properly diluted. The glucomannan concentrations in various dilutedsamples with the highest settling rates were identified in the range of 195.7±21.4mg/L; thus, the results indicate thatthe glucomannan content may be determined from the dilution rate of the aloe gel sample that exhibits the highestsettling rate. This study provides a simple, rapid, and cost-effective assay for the estimation of the glucomannanlevel in raw Aloe vera gel.

In this paper, we present a technique for applying artistic effects to videos based on a pencil rendering technique. First of all, we propose a vectorized line generation method as a primary technique for solving temporal coherence problem which appears when applying some artistic effects to video. In addition, we propose progressive pencil rendering technique which controls detailed range of drawings from rough sketch to detailed drawing for video. Also, regarding that a real color pencil has a limited set of colors, we suggest limited color pencil rendering method. Furthermore we analyze hand drawing pencil drawings, compare them with rendering results and complement some weakness. A series of our works has a meaning of not only technological but also aesthetical consideration for pencil drawings.

Two fractions (permeate and retentate) from fiber free Aloe vera gel were prepared using an ultra-filtration (UF) system with a tubular ceramic membrane (MWCO of 50 kDa), and their dilute solution properties were investigated. The intrinsic viscosities of fiber free Aloe vera gel and the retentate and permeate fractions that were prepared by ultra-filtration were 18.48, 37.74, and 2.01 dL/g, respectively. UF aloe retentate as a polysaccharide rich fraction exhibited polyelectrolyte behavior showing a sharp decrease of intrinsic viscosity by the addition of NaCl. However, as its NaCl concentration increasing to 1 M, its intrinsic viscosity slightly increased due to conformational change caused by the high concentration of NaCl. On the one hand, the intrinsic viscosities of the dilute UF-retentate solution were exponentially decreased with increasing temperature, and the temperature dependency of its intrinsic viscosity showed a sharp discontinuity at 30oC instead of a linear Arrhenius behaviour, indicating a variation of flow mechanism or network rearrangement of a molecular chain. The chain stiffness of polysaccharide rich fraction at bellow and above 0.5 M of NaCl addition was 0.048 and 0.515, respectively, showing different stiffenings and significant conformational change. The coil overlap parameter and critical concentration of UF-retentate were 3.0 and 0.08 g/dL, respectively.

알로에의 최소공정개발 연구의 일환으로 DIS(dewatering & impregnation soaking) 공정에 미치는 온도의 효과를 조사하였다. 삼투탈수는 분자량 4000의 PEG을 삼투제로 하여 이의 40%(w/v) 용액에 두께 0.5 cm의 알로에 잎 슬라이스를 넣고 간헐적으로 교반하면서 2시간 동안 수행하였다. 서로 다른 침지온도(25-55oC)가 DIS의 성능(수분소실 및 고형분 획득)에 미치는 영향을 현미경관찰에 의한 세포조직 구조의 경시변화를 모니터링하면서 조사, 비교되었고, 동역학적으로 분석하였다. 고온(55oC) 처리에서는 저온(25 and 35oC)에서보다 더 높은 수분소실을 보였으나 세포조직의 파괴를 동반하였다. 또, 품질지표값으로서의 glucomannan 함량은 세포조직구조를 잘 유지하였던 침지온도 35oC에서 얻어졌다. 얻어진 시료의 실온건조제품을 재수화시켜 조사하고 동역학적으로 해석한 결과, 재수화 동역학은 Peleg 및 Weibull 모델로 잘 설명할 수 있었다. DIS 제품은 DIS 처리하지 않은 제품에 비해 세포구조 및 재수화성질의 향상을 주어 적정온도하에서 DIS 처리함으로써 최소공정의 알로에 제품을 얻을 수 있음을 확인하였다.

Two fractions (permeate and retentate) from fiber-free Aloe vera gel were separated using ultra-filtration (UF) system with tubular ceramic membrane (MWCO of 50 kDa), and their molecular properties were investigated. The retentate of UF Aloe gel was a polysaccharide-rich fraction containing about 2.3-fold higher polysaccharide content than fiber-free gel. The FT-IR and 1H NMR spectra of this fraction showed the characteristic patterns of β-binding polysaccharide and a higher degree of acetylation indicating a higher level of bioactive polysaccharide content. The molecular weight and polydispersity of polysaccharide fraction from GPC (gel permeation chromatography) were determined to be 36.8-43.9 kDa and 1.24, respectively, indicating a kind of undispersed polysaccharide. From the SEM observation, the surface structure of polysaccharide fraction had a gel cluster-like structure with a convoluted rough surface. The molecular conformation by Congo red assay exhibited a property of helix structure confirming the existence of a higher-ordered structure as a biological activity conformation.

The optimization of dewatering and impregnation soaking (DIS) process for a concentrated Aloe vera product was investigated using Taguchi method in combination with desirability function analysis. Polyethylene glycol (PEG) as osmotic agent was adopted, and soaking temperature (T), immersion time (t), PEG concentration (C), PEG molecular weight (MW), and thickness of Aloe vera leaf slice (x) were selected as affecting variables. L16 (45) orthogonal array was designed by Taguchi method with four parameters such as water loss, solid gain, glucomannan, and anthraquinone contents as objective functions. An overall quality index was transformed from individual objective functions, and was optimized finally. The optimal setting for maximum overall desirability was obtained at 55oC (T), 2 hr (t), 40% w/v (C), 0.5 cm (x), and 4,000 Da. (MW). The obtained overall desirability was 0.7842. The order of affecting factors was T>C>x>MW≈x>t and the experimental results under optimum condition were similar to the prediction of an overall desirability of 0.8384. Also, it was found that the optimized DIS condition could be reproduced for a minimally processed Aloe vera product with high quality.

The analysis of an art trend in the principle dimension starts by observing the object of work in the perspective of formative composition and recognizing it as a universal system. It can be said that it is consistent with an interpretation method for a form theory of formal history by Heinrich Wölfflin, a leading form critic in art criticism. Hence, the purpose of this study was to find out what are the formative principles in Renaissance Classicism as a design principle to be applicable to modern fashion by reviewing the formative characteristics of Renaissance Classicism Architecture with which Wölfflin directly dealt. As for the theoretical literature review, I used Wölfflin's theoretical framework and looked at the Renaissance Classicism Architecture that he studied and examined the possibility of utilizing his theory as a layout principle and the characteristics. As for analysis of design cases, I applied the aforementioned architecture layout principle to modern fashion and conducted case study analysis to delve into distinctive layout principles found in fashion. The study showed that the Renaissance Classicism Architectural Style is marked by linearity, planarity, closing and multiple unity: linearity was expressed in the observation form in fixed frontal view and an emphasis on a tangible silhouette homeogenous and definite line structures; planarity was achieved in the form of paralleled layers of frontal view element, planarity style, and identical and proportional repetition of various sizes.; closing signified the pursuit of complete and clear regularity, and architecture developed in a constructive phase through organizational inevitability and absolute invariability.; multiple unity was expressed in self-completedness and independent parallel of discrete forms and harmony of emphasized individual elements in a totality. Applying these layout characteristics of the Renaissance Classicism Architectural style and to see their individual expressive features, I found out that in adopting layout principles of the Renaissance Classicism Architecture to modern fashion, it turned out to be an emphasis of individual silhouettes, a flattened space, completed objects, organic harmony among independent parts: the emphasis of individual silhouettes was expressed in individual definitiveness of formative lines of clothes in accordance with body joints and an emphasis on formative lines of clothes; the flattened space was marked by single layer structure, planarity of elements of clothes, and listing arrangement by appropriate proportion.; the completedness of the objects was expressed by the stationary state where overall image is fixed, the construction of homogeneous and complete space, and absolute inevitability of internal layout in proportion; lastly, organic harmony of independent parts was stressed in independent completedness of each detail, and organic harmony of the whole. The expressive features would lead to a unique expression style of linear emphasis, proportion, constructive forms, and two-dimensional arrangement. The meaning of this study is follows: The characteristics of art school of thought are given shape by appling & analysing the architectural layout principles of historical art school of thought to modern fashion in the view point of formal construction dimension. The applied possibility of historical art school of thought as the source of inspiration about the fashion design is extended.

Immuno-MemBlot is a technique for detecting, analyzing, and identifying proteins, similar to the Western blot technique but differing in that protein samples are not separated electrophoretically but are spotted through circular or slot templates directly onto the membrane. Recently we developed a new Immuno-MemBlot (IMB) method applying immunoreactions and coloring procedures directly in the wells of MemBlot apparatus, which were connected by canals to perform drainage for reagent application and buffer irrigation. This IMB method was designed to get theimmunoblot results more rapidly and clearly than the previous immunoblot ones. This study is aimed to evaluate the analytical accuracy of IMB using different biological assay. In the sensitivity test of IMB the monoclonal antibody can clearly detect the 30 ng (about 12 pM) of Mucocidin peptide (35 mer), and is also available to detect at least 10 ng (about 4 pM) of Mucocidin peptide (35 mer). The IMB was effective in the quantitative analysis of methothrexate (MTX) assay for cellular apoptosis. And more, this IMB is useful to screen large number of specific samples with ease and accuracy in a short time. In the screenings for the presence of Mucocidin in saliva the quantitative comparison is conspicuous among 48 persons depend on the different conditions ofgender, drinking and smoking habits, and oral diseases. Therefore, it is presumed that, even though the target proteins were partly degraded, a specific epitope can be detected if a monoclonal antibody was still reactive. Conclusively, these data suggest that the IMB can be useful in the primary qualitativeand quantitative analysis of proteins in various fluids, i.e., blood, saliva, tear, urine, etc.

대기 오염은 피부의 산화적 손상, 염증 및 노화를 일으킬 수 있다. 레스베라트롤은 폴리페놀 화합물의 일종으로 항산화, 항염증, 멜라닌 생성 억제 작용 등 다양한 생물학적 활성이 있는 한편 열과 빛에 약한 단점이 있다. 레스베라트릴 트라이아세테이트(RTA)는 레스베라트롤에 비해 안정하고, 피부 안전성과 미백 효능이 보고된 화장품 신소재이다. 본 연구의 목적은 직경 10 μ m 미만 대기 미립자 물질(PM10)에 노출된 인간 표피 각질형성세포(HEK)의 염증 반응에 대한 레스베라트롤과 RTA의 영향을 조사하기 위한 것이다. 배양된 HEK세 포를 레스베라트롤과 RTA의 유무 조건에서 PM10에 노출시키고, 세포 생존율, 반응성 산소종(ROS)의 생성 및 염증성 사이토카인의 발현을 분석하였다. PM10을 처리하였을 때 세포 생존율이 감소하였고 종양괴사인자- α(TNF-α), 인터루킨-1β(IL-1β), 인터루킨-6(IL-6) 및 인터루킨-8(IL-8)의 발현이 증가하였다. 레 스베라트롤과 RTA는 PM10으로 유도된 세포의 사멸과 ROS 생성을 경감시켰다. PM10에 의해 증가되는 여러 염증성 사이토카인의 발현은 레스베라트롤과 RTA에 의해 경감되거나(IL-6), 증진되거나(IL-1β), 변화하지 않았다(TNF-α 및 IL-8). PM10에 의해 유도된 IL-6단백질의 발현이 레스베라트롤과 RTA에 의해 감소되 었다. 본 연구의 결과는 레스베라트롤과 RTA가 대기 미립자 물질에 노출된 피부의 세포 손상과 염증 반응을 조절하는 작용이 있음을 시사한다.