본 연구는 우수한 꿀벌 신품종 육성을 위해 국립농업과학원에서 육성된 재래꿀벌 RX계통을 분양받아 ’23~’24 년까지 부안군 변산면에 위치한 전북농업기술원 잠사곤충시험장 양봉사에서 꿀벌세력, 수밀력, 질병저항성, 온순성, 질병발생, 월동능력을 한라벌 품종과 비교하여 조사하였다. 8~11월 RX계통의 꿀벌세력 감소는 3.9%로 한라벌 39.8% 대비 상대적으로 낮았고, 수밀력, 청소능력, 온순성은 유의적 차이가 없었다. 낭충봉아부패병 등 질병은 전북동물위생시험소에서 병성검정한 결과, 두 시험군 모두 항원이 검출되었으나 임상증상은 없었다. 그리고 월동 중 한라벌 품종은 폐사한 반면, RX계통은 모든 시험군이 월동에 성공하였고, 또한 월동전·후 먹이감 소량이 적어 월동능력이 우수한 것으로 판단하였다. 본 연구는 국립농업과학원 공동연구사업(PJ01504205)의 지원을 받아 수행하였다.

A facile and efficient method was developed to prepare highly stretchable and conductive graphene conductors with wrinkled structures by the mechanical stretching and shrinking of elastomeric substrates, in which graphene inks were printed on a prestretched elastomeric substrate. Stretchable and exfoliated graphene inks were prepared by mixing graphite and Ecoflex in a shear-assisted fluid dynamics reactor. The resultant graphene conductor exhibited excellent stretchability at 150% strain and high electrical conductivity of 64 ± 1.2 S m− 1. The resistance of the conductor did not change in bent, twisted, and stretched states. The resistance did not change during 10,000 cycles of stretching/releasing, with a maximum strain of 150%. Based on the graphene conductor, a stretchable conductometric sensor with a two-electrode configuration was fabricated to measure impedance changes at different concentrations of electrolyte ions. This sensor exhibited a good and linear sensitivity curve (298.61 Ω mM− 1, R2 = 0.999) in bent and stretched states.

This study examines the creation process of the Yeonhaengrok. The Yeonhaengrok or a collection of diplomatic mission records consists of observation and records. Observation can be further divided into direct observation and indirect observation. A diplomatic envoy and his entourage as well as individuals they interacted with function as a knowledge-information provider or conveyor. Records includes those written by Bandang (messenger), Jajegungwan (informal attendants made up of family members of an envoy), and fellow intellectuals, and previous diplomatic mission records and various literatures. It shows the subjects of recording the Yeonhaengrok. The Yeonhaengrok serve to collect and organize knowledge and information obtained during the missions while expanding and reproducing new knowledge and information.

The purpose of this study was to investigate the effects of combined wedge on the range of motion in ankle and knee joint, ankle eversion moment and knee adduction moment, and center of pressure excursion of foot for genu varus among adult men during gait. This study was carried out with 10 adult men for genu varus in a motion analysis laboratory in J university. The subjects of the experiment were measured above 5cm width between the knees on contact of both medial malleolus of ankle while standing. The width of their knees in neutral position was measured without the inversion or eversion of the subtalar joint by the investigator. The subjects of the experiment were ten who were conducted randomly for standard insole, insole with 10° lateral on rear foot wedge, insole at 10°lateral on rear foot and 5° medial on fore foot wedge. Before and after intervention, changes on the range of motion in ankle and knee joint, ankle eversion moment and knee adduction moment, and center of pressure excursion were measured. In order to compare analyses among groups; repeated one-way ANOVA and Scheffé post hoc test were used. As a result, combined wedge group was significantly decreased compared to control wedge group in terms of knee varus angle in mid-stance(p<.05). Combined wedge group was significantly decreased compared to lateral wedge group in terms of ankle eversion moment in whole stance(p<.05). Combined wedge group was significantly decreased compared to lateral wedge group in terms of knee adduction moment in whole stance(p<.05). Combined wedge group was significantly decreased compared to lateral wedge in terms of center of pressure excursion in whole stance(p<.05). The results of this study suggest that combined wedge for genu varus decreased ankle eversion moment and knee adduction moment upon center of pressure excursion. We hypothesize that combined wedge may also be effective in the protection excessive ankle pronation.

Sirtuin proteins are evolutionary conserved Sir2-related NAD+-dependent deacetylases and regulate many of cellular processes such as metabolism, inflammation, transcription, and aging. Sirtuin contains activity of either ADP-ribosyl-transferase or deacetyltranfease and their activity is dependent on the localization in cells. However, the expression pattern of Sirtuins has not been well studied. To examine the expression levels of Sirtuins, RT-PCR was performed using total RNAs from various tissues including liver, small intestine, heart, brain, kidney, lung, spleen, stomach, uterus, ovary, and testis. Sirtuins were highly expressed in most of tissues including the testis. Immunostaining assay showed that Sirt1 and Sirt6 were mainly located in the nucleus of germ cells, spermatocytes, and spermatids in the seminiferous tubules, whereas Sirt2 and Sirt5 were exclusively present in the cytoplasm of germ cells and sperma-tocytes. Our results indicate that Sirtuins may function as regulators of spermatogenesis and their activities might be dependent on their location in the seminiferous tubules.

The purpose of this study was to investigate the effects of joint position on the distraction distance in patients with adhesive capsulitis of glenohumeral joint. The study was conducted upon 20 adults in their 40's with the findings of adhesive capsulitis of glenohumeral joint. These subjects were subdivided into 3 groups, which were a group with neutral position(n=7), second group with resting position(n=7) and third group with end-range position( n=6). After having the subject wearing sleeveless shirts exposing armpit and lying straight on the plinth, a physical therapist with OMT qualification pulled glenohumeral joint at the Grade Ⅲ of Kaltenborn-Evjenth traction; and the distance between glenoid fossa and humeral head was measured with ultrasound. Following the application of traction, the group with resting position(.67±0.29) exhibited the longest distance between humeral head and glenoid fossa, and it was followed by neutral position(.50±0.25) and end-range position(.35±.21) in this order. From the comparison of these groups, there was no significant difference in distraction distance between resting position and neutral position; and there was again no significant difference in distraction distance between end-range position and neutral position. However, there was a significant difference in distraction distance between end-range position and resting position(p<.05). Upon application of the Grade Ⅲ of Kaltenborn-Evjenth traction, it was evident that the distance between humeral head and glenoid fossa can be varied depending on the location of the joint.

Sacbrood disease is a viral disease on honey bee larvae Apis cerana. Diseased larvae fail to pupae and to be dead at old larvae and pre-pupae stage. Currently, there is no remedy to control sacbrood disease. In this study we conducted to observe sacbrood disease on Apis cerana colonies from June to September, 2014 at the A. cerana apiary of NAAS, and using biological measure to treat this disease. Our study results were showed that sacbrood disease infected A. cerana colonies in all months of observation. The percentage of infected colonies was from 33.3% up to 100%. Controlling sacbrood disease by requeen measure, the percentage of recovered colonies was 57.1 % while of this by cage queen measure was only 28.6 %.

In this study we conducted to rear worker honey bee (Apis cerana) from larvae to adult stage in the laboratory by using plastic well plates. Our study results were showed that honey bee larvae Apis cerana could be reared in the laboratory. The adult worker bee started to emerge on day 17 from grafting. The emergence of worker bee peak on day 18 and declined thereafter. The average survival rate from larvae to pre-pupae stage was 74.6%. The average survival rates from pre-pupae to adult stage and from larvae to adult stage were 40.7 % and 30.4 % respectively.

Asian is rich in honey bee species and genetic diversity. Among the difference native honey bee species, Apis cerana is very diversity of subspecies and distribution as well. Until now, nine A. cerana subspecies have been named. However, natural diversity of this species is being declined by threats such as pest, disease, deforestation, pesticide positioning and climate change. Therefore, the understanding of morphological characteristics of A. cerana is viral for maintaining biological diversity. In this paper we give an overview of method that are used for distinguish honey bee A. cerana subspecies and ecotype that can contribute to recognize genetic origin of colonies for conservation and breeding purpose. Base on morphmetric method currently in use, we outline strategies for sampling and measuring morphological characteristics on A. cerana.

Pine wilt disease, caused by pine wood nematode, Bursaphelenchus xylophilus (Steiner et Buhrer) Nickle, has become the most serious threat to pine trees in Korea since first reported in 1988. Pine wood nematode is transferred to uninfested trees by Monochamus alternatus (Coleoptera: Cerambycidae) (vector). A typical controlling method against vectors in Korea is fumigation of the dead trees infested by vector M.alternatus and B.xylophilus using metam-sodium SL(25%). However, this method is harmful to the environment because of the chemical contamination and vinyl waste in the mountain afterward. Portable Bark Remover (PBR) was contrived to reduce these environmental problems. The vectors oviposite under the bark of the freshly dead trees only. Debark infested trees prohibited the vectors from oviposite and finally, vectors can not complete their life cycle. The PBR is a newly designed as a debarking device that is equipped on top of the chain saw engine allowing ease and rapid debarking of the infested trees at the controlling field. Debarking of freshly-dead trees can eliminate egg-laying and hatch places where between the wood and bark from vectors. The new method for vector controlling using the debarking device is expected to lower-price and high-efficiency comparing with other conventional methods such as “crushing”, “burning” and “fumigation”.

Trunk injection of nematicides was applied to prevent pine wilt disease (PWD). Although the trunk injection of nematicides was effective to PWD prevention, it was not effective to a vector, Monochamus alternatus. Thus, This study was investigated for the insecticidal activity and the effect of oviposition deterrence by injecting to pine trees with systemic insecticides such as Acetamiprid SC 10%, Imidacloprid DC (20%), and Thiamethoxam DC (15%). As a results, mortality of M. alternatus adults was 100% at 56 days after trunk injection. Percentage of M. alternatus adults moved to a young black pine tree by trunk injection of Imidacloprid DC (20%) and Thiamethoxam DC (15%) in screen cage (4.0×2.0×2.5m) was 76.7% and 70.0%, respectively. But the mortality of M. alternatus adults showed 100%. Percentage of M. alternatus adults moved to the treated and untreated young black pine trees by trunk injection of Acetamiprid SC (10%) in screen cage (4.0×2.0×2.5m) were 25.9% and 49.5% at 1st day and 3rd day after treatment, respectively. Percentage of M. alternatus adults oviposited to treated pine logs at 3month after trunk injection of liquid mixture of Acetamiprid SC (10%) and Emamectin benzoate EC (2.15%) in screen cage (72×72×100㎝) was 25%. But, untreated pine logs was 100%

The the peach pyralid moth, Dichocrocis punctiferalis causes the most serious damage to chestnut in Korea. This study was conducted to compare the efficiency of control with aerial spraying(AS350-B2 Helicopter, 15m/flight altitude, Nozzleorifice D6, Fenitrothion EC 50% 1ℓ/ha), sex pheromone trap(25 Wing traps/ha, 75:25 mixture of (E)-10-hexadecenyl aldehyde (E10-16:Al) and (Z)-10-hexadecenyl aldehyde, 1㎎/trap), and crown spraying(BF530, Fenitrothion EC 50% 1ℓ/ha) against D. punctiferalis at chestnut orchards. After chestnut harvest in Autumn, the damaged rate(%) by D. punctiferalis showed 29.6±1.7 in aerial spraying, 27.8±8.4 in sex pheromone trap, and 8.9±3.8 in crown spraying, respectively. The crown spraying showed the highest control effect among the 3 control methods. The other methods were similarly effective.

모의지하수중에서 가압경수로형 사용후핵연료로부터 세슘의 침출률에 미치는 공학적 방벽 영향을 규명하기 위하여 지난 약 6년간 벤토나이트 또는 금속시편등의 존재여부에 따라 침출시험을 수행하였다. 침출률은 시간이 경과함에 따라 지수함수적으로 감소하는 경향을 나타내었으며, 적정시간이 경과한 다음부터는 일정한 값에 수렴하는 경향을 나타내었다. 벤토나이트 또는 금속시편이 있을 경우 세슘의 누적누출분률은 선형적으로 증가하였으나, 이들이 없는 경우의 누적분률은 급격히 증가한 다음 서서히 증가하는 경향을 보였다. 이 누적분률에서 갭에 존재하는 세슘의 재고량을 제한 값은 공학적 방벽이 존재하는 경우의 누적분률에 거의 근접하였다. 이러한 결과들은 사용후핵연료 중 세슘의 초기누출분률은 갭 중 세슘의 재고량에 의존하지만, 세슘의 장기침출률은 공학적 방벽에 거의 영향을 받지 않음을 암시해 주고 있다. 그러나 세슘의 초기침출률은 공학적 방벽의 지연효과로 감소될 수 있을 것이다. 그리고 처분장에서 사용후핵연료 중 세슘의 장기침출률은 를 넘지않는 범위 내에서 일정한 값을 가질 것이다.