본 연구에서는 예비 마스터가드너를 선발, 정보를 전달하고 활동을 촉진시켜 프로그램 진행을 위한 실질적인 관리를 담당 하는 코디네이터 양성을 위해 데이컴 분석을 통하여 마스터가드너 코디네이터의 전문성 기준과 직무모형을 제시하였다. 1, 2차 데이컴 위원회를 통해 임무, 작업, 작업 요소를 도출하고 작업에 필요한 지식, 기술, 태도를 확정하였다. 도출된 임무 및 작업 등을 바탕으로 마스터가드너 교육을 담당하고 있는 시군 농업기술센터 농촌지도사를 대상으로 직무 수행평가를 시행하였다. 마스터가드너 코디네이터 직무분석 결과, <A. 정원문화 이해>, <B. 정원기술 이해>, <C. 봉사활동 이해>, <D. 교육과정 개발>, <E. 교육과정 운영>, <F. 교육사후 관리>, <G. 전문성 개발> 등 7개의 임무를 도출하였다. 또한 <A. 정원 문화 이해>의 4개, <B. 정원 기술 이해>의 4개, <C. 봉사 활동 이해>의 6개, <D. 교육과정 개발>의 6개, <E. 교육과정 운영>의 8개, <F. 교육 사후 관리>의 6개, <G. 전문성 개발> 의 7개 작업까지 총 41개의 작업과 173개의 작업 요소를 도출 하였다. 이를 토대로 작업과 지식, 기술, 태도의 관계를 분석 하고 매트릭스로 제작하여 마스터가드너 코디네이터 직무 활동에 사용하도록 하였다. 본 연구의 의의는 마스터가드너 코디네이터의 역할과 활동 범위를 설정을 위한 직무모형을 제안함으로써 향후 마스터가드너 코디네이터 직무교육 표준화 기준을 수립하고 일자리 영역을 구축하는 등 후속 연구를 촉진 할 수 있는 토대될 수 있을 것이라 사료된다.

This study aimed to examine the effect of a mild elevation in serum cholesterol level in a porcine coronary overstretch restenosis model using a balloon angioplasty catheter or drug-eluting coronary stent. Pigs were divided into two groups and were fed a commercial normal diet (CND, n = 4) or a high-fat diet (HFD, n = 4) for 5 weeks. Coronary overstretch injury by balloon angioplasty or stent implantation was induced in the left anterior descending and left circumflex artery after 1 week of feeding. Histopathological analysis was performed at 4 weeks after coronary injury. During the experiment, the total cholesterol level in the HFD group increased by approximately 44.9% (from 65.9 ± 3.21 mg/dL at baseline to 95.5 ± 9.94 mg/dL at 5 weeks). The lumen area in the CND group was reduced in comparison with that in the HFD group after balloon angioplasty. After stent implantation, the injury score showed no significant difference. There were significant differences in the neointimal area (2.7 ± 0.33 mm2 in the CND group vs. 3.3 ± 0.34 mm2 in the HFD group, p<0.05), lumen area (2.6 ± 0.54 mm2 in the CND group vs. 2.0 ± 0.33 mm2 in the HFD group, p<0.05), and percent area stenosis (52.0 ± 7.96% in the CND group vs. 62.4 ± 5.15% in the HFD group, p<0.05). Body weight change was not different between the two groups. Increased serum cholesterol level activated vascular smooth muscle cell proliferation in the porcine coronary overstretch model.

Acute myocardial infarction (AMI) is considered the major cause of mortality in the world. Tremendous animal studies are performed to develop novel therapeutics, and this study aimed to induce porcine myocardial infarction model by using polyethylene terephthalate (PET). Coronary guidewire was placed in left anterior descending artery (LAD). The balloon angioplasty catheter was inserted at the back of the PET. The balloon catheter was carefully pushed forward, until the balloon marker was located in mid-LAD. Coronary angiography was performed pre- and post-occlusion at 28 days by C-arm. Histologic analysis of heart tissue was performed 28 days after inducing AMI. Thirty three pigs were anesthetized and underwent percutaneous coronary catheterization. All pigs were successfully embolized in mid-LAD by PET. Fifteen pigs died due to ventricular fibrillation during post-anesthetic recovery time, and overall experiment mortality was 45.5%. In 2,3,5- triphenyl tetrazolium chloride staining, gross finding of the ischemic heart lesion showed firm and white area of infarction associated with the apex and left ventricular posterior wall. Infarct on H&E-stained sections demonstrated a region without myocytes and rich with cardiomyocyte with atypical nuclei. Successful induction of AMI by using PET may provide the pathophysiological information of ischemic heart disease and improvement of therapy development for AMI.

Changes in land use and increase in urban energy consumption influence urban life. This study analyzed the characteristics and patterns of urban heat and presents management schemes to generate a comfortable and sustainable urban environment. The study aimed to demonstrate the positive effects of artificial ground greening on improving the microclimate through evapotranspiration using perennial herbs. We have designed a chamber that could control constant temperature and humidity, measure temperature reductions in each plant and changes in sensible heat and latent heat. This study identified Sedum kamtschaticum as the most effective plant in controlling temperature. At 22℃, 3.2℃ temperature reduction was observed, whereas four other plants showed a 1.5℃ reduction. At 25℃, 2.0℃ temperature reduction was observed. On the other hand, the use of Sedum sarmentosum resulted in the lowest effect. Zoysia japonica is the most commonly used ground covering plant, although the temperature reduction of Lysimachia nummularia was more effective at high temperature conditions. Sensible heat and latent heat were calculated to evaluate the thermal performance of energy. At a temperature >30℃, L. nummularia and S. sarmentosum emitted high latent heat. In this study, we analyzed the thermal performance of green roof perennial plants; in particular, we analyzed the evapotranspiration and temperature reduction of each plant. Since the substrate depth and types, plant species, and seasonal change may influence temperature reduction and latent heat of green roofs, further studies are necessary.

Anthricin (Deoxypodophyllotoxin), a naturally occurring flavolignan, has well known anti-cancer properties in several cancer cells, such as prostate cancer, cervical carcinoma and pancreatic cancer. However, the effects of Anthricin are currently unknown in oral cancer. We examined the anticancer effect and mechanism of action of Anthricin in human FaDu hypopharyngeal squamous carcinoma cells. Our data showed that Anthricin inhibits cell viability in a dose- and time-dependent manner (IC50 50 nM) in the MTT assay and Live & Dead assay. In addition, Anthricin treated FaDu cells showed marked apoptosis by DAPI stain and FACS. Furthermore, Anthricin activates anti-apoptotic factors such as caspase-3, -9 and poly (ADP-ribose) polymerase (PARP), suggesting that caspase-mediated pathways are involved in Anthricin- induced apoptosis. Anthricin treatment also leads to accumulation of the pro-apoptotic factor Bax, followed by inhibition of cell growth. Taken together, these results indicate that Anthricn-induced cell death of human FaDu hypopharyngeal squamous carcinoma cells is mediated by mitochondrial-dependent apoptotic pathway. In summary, our findings provide a framework for further exploration on Anthricin as a novel chemotherapeutic drug for human oral cancer.

Dietary and lifestyle modifications are widely prescribed to prevent recurrence of urolithiasis, although little is known about the clinical and demographic factors associated with patient compliance and urinary metabolic changes. The present study assessed the clinical and demographic factors influencing compliance with a modified diet and lifestyle in first-time ureteric stone formers as well as determined the effects of compliance on urinary stone risk factors. We retrospectively reviewed the medical records of 53 patients presenting with ureteric calcium stones. Using a self-completed questionnaire, patients were classified according to compliance with seven recommendations for modifying diet and lifestyle into good compliance group (complied with ≥ three recommendations) and poor compliance group. Before (on a random diet) and after prescribing the modifications, 24 hour urine samples were collected from those in the good and poor compliance group. The stone size at presentation and initial treatment modality were closely associated with patient compliance (P=0.019, P=0.027, respectively). Citrate excretion significantly increased in the good compliance group after adopting modifications (P=0.012), whereas the poor compliance group did not show a statistically significant difference. Moreover, patients in the poor compliance group showed significantly increased urinary calcium excretion by the end of the study (P=0.040). After adjustments for age, sex, body mass index, and metabolic abnormality status, poor compliance was found to be an independent risk factor for persistence or development of hypocitraturia (OR: 3.885; 95% CI: 1.102~13.694; P=0.035). In conclusion, our results imply that patient education programs regarding diet and lifestyle should be tailored to the individual’s clinical and demographic characteristics.

This work was conducted to assess the use of Near-infrared reflectance spectroscopy (NIRS) as a technique to analyze nutritional constituents of Distillers dried grain with solubles (DDGS) and corn quickly and accurately, and to apply an NIRS-based indium gallium arsenide array detector, rather than a NIRS-based scanning system, to collect spectra and induce and analyze calibration equations using equipment which is better suited to field application. As a technique to induce calibration equations, Partial Least Squares (PLS) was used, and for better accuracy, various mathematical transformations were applied. A multivariate outlier detection method was applied to induce calibration equations, and, as a result, the way of structuring a calibration set significantly affected prediction accuracy. The prediction of nutritional constituents of distillers dried grains with solubles resulted in the following: moisture (R2=0.80), crude protein (R2=0.71), crude fat (R2=0.80), crude fiber (R2=0.32), and crude ash (R2=0.72). All constituents except crude fiber showed good results. The prediction of nutritional constituents of corn resulted in the following: moisture (R2=0.79), crude protein (R2=0.61), crude fat (R2=0.79), crude fiber (R2=0.63), and crude ash (R2=0.75). Therefore, all constituents except for crude fat and crude fiber were predicted for their chemical composition of DDGS and corn through Near-infrared reflectance spectroscopy.

The purpose of this study is to examine the changes in bone mineral density in long-stay patients of a geriatric hospital in relation to physical therapy, thereby providing basic data for preventing the onset or deterioration of osteoporosis in inpatients of geriatric hospitals or relevant facilities. The subjects of this study were 133 elderly patients who had been in H geriatric hospital in Yongin-si for more than four years. Their bone mineral density T-scores at admission and those after four years were measured to compare and analyze the changes in bone mineral density in relation to physical therapy. According to the results, the decrease in T-score for the group without physical therapy was greater by .40 than that for the group with physical therapy, indicating a statistically significant difference between the two groups(p<.01). Accordingly, physical therapy proved to be effective in preventing bone mineral density loss and maintaining the current density.

This study aims to reveal how EA affects BAX and NF-kB involved in cell deaths from global ischemia, and to do this, observes the changes of BAX and NF-kB caused by EA application after transient global ischemia. The experimental method is to give rise to global ischemia and apply EA to 27 SD rats with the particulars of being six-week-old, male, around-300 gram-weighing, and adapted to laboratory environment for more than a week, and divide them into three groups, that is, GV20 EA group(n=9), L14 EA group(n=9), no-treatment GI group(n=9), and then observe their changes of BAX and NF-kB at the time lapse of 6 hours, 9 hours and 12 hours after ischemia, using western blotting. The numerical decrease of BAX expression at the time lapse of 9 hours after EA application, though not statistically significant, was observed in GV20 EA group and L14 EA group, and the NF-kB expression appeared statistically significant decrease in GV20 EA group and L14 EA group, but the expression was higher in the group with EA application. Therefore, EA application at the early phase of global ischemia is considered to affect BAX and NF-kB and play a positive role in decreasing apoptosis and cell deaths by inflammation.

This study was performed to investigate the effects of Needle Electrode Electrical Stimulation(NEES) on ischemia-induced cere˗ brovascular accidents. After obstruction and reperfusion of arteries in white mice, the amounts of necrosis and inflammation related sub˗ stances Bax, IL-6, Caspase-3, and COX-2 were measured in neurons of the fore-brain. The following results were obtained. This study used 21 male specific pathogen free(SPF) SD rats, 8 weeks of age and approximately 300g in weight. Each exposed artery was completely occluded with non-absorbent suture thread and kept in that state for 5 minutes. The sutures were then removed to allow reperfusion of blood. Test group is control group(common carotid artery occlusion models), a GI(underwent common carotid artery occlusion), and NEES(underwent NEES after artery occlusion). The GI and NEES groups were given 12, 24, or 48 hours of reperfusion before NEES. NEES device(PG6, ITO, Japan, 9V, current, 2Hz) was used to stimulate the bilateral acupoint ST36 of the SD rats for 30 minutes while they were sedated with 3% isoflurane. An immuno-his˗ tochemistry test was done on the forebrains of the GI induced rats. Both Bax and Caspase-3 immuno-reactive cells, related to apoptosis, were greater in the GI than the NEES group. Cox-2 and IL-6 immuno-reactive cells, related to inflammation, were greater in the GI and NEES groups than the control group. We can expect that applying NEES after ischemic CVA is effective for preventing brain cells from being destroyed. And we can conclude NEES should be applyed on early stage of ischemic CVA.