목적 : 본 연구는 만 50세 이상 성인에서 녹내장, 황반변성, 당뇨병성망막병증의 의사진단여부와 삶의 질의 관 련성을 알아보고자 하였다. 방법 : 국민건강영양조사 제7기(2016~2018) 자료 중 만 50세 이상의 성인으로 녹내장, 황반변성, 당뇨병성망 막병증의 의사진단여부에 따라 현재의 건강상태를 묻는 EQ-5D와의 관계를 분석하였으며, p<0.050인 경우 유의 한 값으로 판단하였다. 결과 : 녹내장, 황반변성, 당뇨병성망막병증에 영향을 주는 요인은 공통적으로 연령이었다. 이들 안질환과 삶의 질의 관계에서 녹내장과 당뇨망막병증은 일상 활동에서의 불편함이 있는 것으로 보였으나 통계적 유의성은 없었 다. 반면 황반변성은 자기관리 능력을 상실시킬 수 있는 위험이 유의하게 큰 것으로 나타났다[Odds ratio: 2.56(95% CI:1.05-6.25)]. 결론 : 본 연구에서 녹내장, 황반변성, 당뇨망막병증과 같은 주요 안질환은 연령과 관련이 있으며, 황반변성은 자기관리능력과 같은 삶의 질을 저하시킨다는 것을 확인하였다

목적 : 본 연구는 안경원의 운영관리 요소가 고객만족도 및 가맹본사만족도, 그리고 경영성과에 미치는 영향을 체계적으로 분석하였다. 방법 : 설문조사 자료는 2021년 5월 12일~28일까지 전국의 안경원 원장들을 대상으로 수집한 총 258개를 활 용 분석하였다. 결과 : 안경원 유형(대형브랜드, 중소형브랜드, 일반안경원)에 관계없이 모든 안경원은 공통적으로 고객관리를 중요한 성공요소로 인식하고 있는 것으로 나타났다. 유형별 안경원의 경영성과 요소는 전체적으로 ‘고객만족도’가 높게 나타난 반면, ‘매출성과’는 낮게 나타났다. 통계적으로는 ‘가맹본사만족도’, ‘매출성과’, ‘고객 수’에서 p<0.010 수준에서 집단 간에 유의한 차이가 있는 것으로 나타났다. 마지막으로 안경 프랜차이즈 브랜드별로 운영관리 성공 요인을 분석한 결과, 브랜드별로 안경원의 성공요인에 차이가 있다는 것을 확인하였다. 특히, 대형 A브랜드는 가 맹본사 교육 및 가맹본사 지원관리에서 훨씬 우월한 것으로 나타났다. 결론 : 본 연구의 결과로 대형브랜드 안경원이 브랜드 이미지를 강조하며 안경사 전문성과 접근성이 좋은 우량 한 점포위치를 선호하는 반면, 중소형브랜드는 마케팅역량을 가장 중요한 성공 요소로 인식하고 있는 것으로 생각 된다.

목적: 본 연구는 연령증가에 따른 안경굴절검안에 있어서 조절반응과 조절지체 간의 변화량의 영향 관계를 검정하는데 있다. 방법 : 연령증가에 따른 원점과 근점의 자극에 대응하는 조절 반응점 (ARf, ARn)을 연구하기 위해서, 선조 검영기를 사용하여 정시 74개 (남: 37, 여: 37)의 단안을 대상으로 굴절검사를 진행하였다. MEM 0.4 시력카드의 스넬린 (Snellen chart) E 단일문자를 검영기헤드 (No 18235, WelchAllyn, USA) 전면에 부착한 선조 검영기를 사용하여 조절 반응을 확인하였다. 결과 : 연령증가에 따른 원점 조절반응의 경우Ⅰ>Ⅱ, Ⅲ, Ⅳ의 유의한 결과를 보였고, 나머지 근점 조절반응과 조절지체는 Ⅰ, Ⅱ, Ⅲ<Ⅳ의 결과를 보여주었다. 연령증가에 따른 원점 조절반응과 조절지체 간의 통계적 조절효 과는 Ⅰ·Ⅱ〉Ⅰ·Ⅲ〉Ⅰ·Ⅳ의 유의한 결과를 보였다. 결론 : 40대 이후 연령의 증가에 따른 시축상의 원점 조절반응의 절댓값과 조절지체는 각각 감소하고 증가함에 따라 원거리 안경굴절검사를 위한 안와 내 조절반응의 기준점을 달리 적용하여야 한다.

Osteoporosis is a common disease characterized by bone mass reduction, leading to an increased risk of bone fracture, and it is caused by an imbalance of osteoblastic bone formation and osteoclastic bone resorption. Current osteoporosis drugs aim to reduce the risk of bone fracture, either by increasing osteoblastic bone formation or decreasing osteoclastic bone resorption. However, osteoblasts and osteoclasts are closely coupled, such that any reagent altering the differentiation or activity of one eventually affects the other. This tight coupling between osteoblasts and osteoclasts not only limits the therapeutic efficacy but also threatens the safety of osteoporosis drugs. This review will discuss the biological mechanisms of action of currently approved medications for osteoporosis treatment, focusing on the osteoblast–osteoclast coupling.

Quercetin is a natural flavonoid phytochemical that is extracted from various plants. Having an advantages due to its varied biological properties, such as anti-inflammatory, anti-viral, anti-oxidant, and anti-cancer effects, quercetin is used to treat many diseases. Recently, it has been reported that autophagy inhibition may play a key role in anti-cancer therapy. Therefore, in this study, we investigated the molecular mechanisms and anti-cancer effects of quercetin in human osteosarcoma cells via autophagy inhibition. We ascertained that quercetin inhibited cell proliferation and induced cell death, these process is demonstrated that apoptosis via the mitochondrial pathway and the caspase cascade. Quercetin also induced autophagy which was inhibited by 3-MA, autophagy inhibitor and the blockade of autophagy promoted the quercetin-induced apoptosis, confirming that autophagy is a pro-survival process. Thus, these findings demonstrate that quercetin is an effective anti-cancer agent, and the combination of quercetin and an autophagy inhibitor should enhance the effect of anti-cancer therapy.

The scientific questions arising from the ability of certain species, but not others, to massively regenerate their bodies are among the most fascinating and challenging confronting modern cell and developmental biologists today. The tremendous implications of this research area for human medicine and tissue engineering are obvious. Yet many other animals exhibit robust regenerative capabilities, including “lower” vertebrates such as amphibians, and invertebrates such as echinoderms, flatworms and annelids. In the extreme case, some species can reproduce vegetatively indefinitely. Such animals must contain the operational equivalent of immortal, totipotent somatic stem cells. From invertebrates to the higher vertebrates, their metabolic pathway, developmental regulatory genes, and intercellular signaling pathways are evolutionary conserved. With these, study on regeneration is an ingenious, powerful model system for studying the post-embryonic development and innate immunity mechanisms. Here, I will discuss the processes of immunemediated gut injury and regeneration using annelid regeneration model system.

In this study, we attempt to synthesize the cordierite from the reaction of fly-ash, alumina, silicon dioxide, and magnesia powders. For the purpose of air purification, the honeycomb filter with porous cordierite was fabricated from the combination of synthetic cordierite and pore forming agent. Fabricated porous cordierite honeycomb was prepared with high porosity (58%), and good compressive strength (69MPa).

In this talk, I will describe recent advances in the field of metazoan comparative genomics and regeneration, the insights into the marine animal genomic and annelid regeneration. The scientific questions arising from the ability of certain species, but not others, to massively regenerate their bodies are among the most fascinating and challenging confronting modern cell and developmental biologists today. The tremendous implications of this research area for human medicine and tissue engineering are obvious. Yet many other animals exhibit robust regenerative capabilities, including "lower" vertebrates such as amphibians, and invertebrates such as echinoderms, flatworms and annelids. In the extreme case, some species can reproduce vegetative indefinitely. Such animals must contain the operational equivalent of immortal, totipotent somatic stem cells. From invertebrates to the higher vertebrates, their metabolic pathway, developmental regulatory genes, and intercellular signaling pathways are evolutionary conserved. With these, study on regeneration is an ingenious, powerful model system for studying the post-embryonic development, innate immunity mechanisms, and primordial germ cells (PGCs).

This study presents a system dynamics methodology to evaluate quantitatively the effect of the Korean government’s development policy, such as tax reductions, on the industrial economy. System dynamics is often perceived as an optimized means to identify the dynamic inter-relationships among various factors of development policies, and in particular the industrial characteristics and uncertainties of the coastal shipping industry. The results of simulations used in this study shows that the impact of development policies such as tax reductions would increase shipping demand for about 4 years, and that tax incentives could raise the demand volume for cabotage cargo from 5.26 to 11.11%, through the available freight- down by 90∼95% points. The system dynamics approach used in this paper represents an initial attempt to use this methodology in studies of the coastal shipping industry. On the basis of our simulations, the industrial effects of other development policies, such as ship financing support, investment of social overhead, or crew supply, could also be analyzed effectively. Additionally, it should be possible to extend these results by developing a comprehensive model encompassing these various analyses.

Ecological toxicity testing of the whole-effluent from the ozone ballast water treatment system was conducted as specified in the quality assurance project plans (QAPP). The growth inhibition test with microalgae, acute aquatic toxicity test with the Rotifer reproduction, toxicity test (or population growth) with the Rotifer, survival and growth toxicity test with larval fish and sediment toxicity test with amphipod were carried out to evaluate ecological toxicity on the movile test barge.

Vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) are the most important angiogenic molecules associated with tumor-induced neovascularization. This study was carried out to investigate inhibitory effect of extracts from root of Rehmannia glutinosa LIBOSCHITZ (Rehmannia Radix and Rehmannia Radix Preparata) on endothelial cell proliferation. The methanol extracts from the medicinal herb were fractionated into n-hexane, ethyl acetate, n-butanol and aqueous fractions. Among the four fractions, the n-butanol fraction from R. Radix on exhibited highly effective inhibition (≈79% inhibition) on the binding of KDR/Flk-1-Fc to immobilized VEGF165 and then ethyl acetate fraction from R. Radix (≈45% inhibition) at the concentration of 100 μg/ml. The n-butanol fraction efficiently blocked the VEGF- and bFGF-induced HUVEC proliferation in a dose-dependent manner, but did not affect the growth of HT1080 human fibrosarcoma cells. The n-butanol fraction more efficiently blocked the binding of KDR/Flk-1-Fc to immobilized VEGF165 and VEGF- and bFGF-induced human umbilical vein endothelial cell proliferation than the fraction from R. Radix Preparata. Our results suggest that Rehmannia Radix may be used as a candidate for developing anti-angiogenic agent.

This study was carried out to investigate inhibitory effect of extracts from Artemisia capillaris Thumb. on maltase, sucrase, α-amylase, nonspecific α-glucosidase, and postprandial hyperglycemia. Methanol extract and organic solvent (n-hexane, ethyl acetate, butanol, aqueous) fractions from the medicinal herb were determined for the inhibitory activities against maltase, sucrase and α-amylase. The methanol extract from A. capillaris strongly inhibited maltase (57%) and α-glucosidase (72%) at the concentration of 100 μg/ml. Among the four fractions (n-hexane, ethyl acetate, butanol, aqueous) examined, the butanol fraction from A. capillaris showed potent inhibitory effects on maltase (73%), sucrase (33%), and α-amylase (75%) at the concentration of 100 μg/ml. The butanol fraction from Artemisia capillaris also exhibited significant reductions (20%) of blood glucose elevation in mice loaded with maltose. These results suggest that the extract from Artemisia capillaris can be used as a new nutraceutical for inhibition on postprandial hyperglycemia

A method for plant regeneration via organogenesis from Pelagonium inquinans leaf disc has been developed. Mature leaf explants were collected from field-grown plants and used for the induction of adventitious shoot regeneration on Murashige and Skoog (MS) medium supplemented with 3% (w/v) sucrose plus plant growth regulators. Maximum shoot organogenesis, with 11.8±1.5 shoots (98.6%) per leaf disc, was obtained with 2 mg/l N6-benzyladenine (BA) and 0.5 mg/l α-naphthyleneacetic acid (NAA) in 30 days. For rooting, the in vitro proliferated and elongated shoots were excised into 1.5-2 cm in length microcutting, which were plated individually on an half-strength MS (1/2MS) medium supplemented with 2% (w/v) sucrose plus various concentrations of indole-3-butyric acid (IBA). Shoots rooted with a frequency of 100% following culture on 1/2MS medium containing 0.5 mg/l IBA.

An efficient plant regeneration of C. asiatica was achieved from organogenesis using petiole explants of in vitro plantlet on MS basal medium controled with different plant growth regulators (NAA,2,4-D, IAA kinetin, and BA). Best results that 50%, efficiency of regeneration per explant for regeneration were obtained with IAA 17.13 μM and BA 8.9 μM. Formation of adventitious shoots via organogenesis from the petiole explant was verified by histological sectioning of plantlets. Regenerated plants were transplanted into soil.

Aconitine alkaloids produced from cell suspension cultures of Aconitum napellus for the first time. The effects of various culture conditions on cell biomass and aconitine accumulation in cell suspension cultures were investigated. Suspension cell cultures of A. napellus were established by transferring callus tissues from leaf explants onto liquid MS medium supplemented with 1 mg/l NAA and 0.1 mg/l kinetin. Among the culture media tested, MS medium had a pronounced effect on cell growth and aconitine accumulation. The maximum dry cell weight was obtained at inoculum size of 3 g (FCW) per flask and in MS medium supplemented with 5% sucrose after 8 weeks. The addition of salicylic acid (SA) and yeast extract (YE) in the MS medium enhanced aconitine accumulation. Using a proper combination of culture condition and supplements, aconitine content could reach 0.043% (dry weight basis), that was 2.5~3 fold higher that detected in control cultures.

A protocol is described for rapid multiplication of Zanthoxylum piperitum DC. (Rutaceae), an important aromatic and medicinal plant, through shoot-tip explant cultures. Murashige and Skoog (MS) medium supplemented with various concentrations of N-6-benzyladenine (BA), N-6-benzylaminopurine (BAP) and thidiazuron (TDZ), in single or in combination with α-naphthaleneacetic acid (NAA), was used to determine the rate of shoot proliferation. N-6-benzyladenine (BA) used at 0.5mg/l, was the most effective in initiating multiple shoot proliferation at the rate of 23 microshoots per shoot-tip explants after 40 days of culture. Shoot multiplication increased 1.2-fold in each successive subculture. Induction of rooting (98%) was achieved by transferring the shoots to the same basal medium containing 2 mg/l indole-3-butyric acid (IBA). Plantlets went through a hardening phase in a controlled growth chamber, prior to in vivo transfer. These results represented that possible application for the mass production of plantlets through in vitro culture system of Zanthoxylum piperitum DC.