농촌진흥청 국립원예특작과학원에서는 2017년 빅립 형태의 분홍색계 팔레놉시스 ‘Lovely Angel’을 개발하였다. 2009년 백색 팔레놉시스 P. ‘Timonthy’와 빅립을 가지는 분홍색 P. World Class ‘Big Foot’을 모본과 부본으로 교배하였다. 2013년 생육이 우수하고 화색, 화형, 꽃대수 등 개화 특성이 우수한 ‘09056-12’를 개체선발하여 기내 화경배양을 통해 증식하였다. 2014년부터 2017년에거쳐 1차, 2차 특성검정을 통해 품종의 안정성과 균일성을 확인하여 ‘Lovely Angel’을 육성하였다. 이 품종은 밝고 선명한 보라빛 분홍색(RHS, PVG81C)을 띄며, 줄 무늬를 가지고 있고 설판이 큰 빅립 형태가 특징적이다. 안아피는 형태의 꽃은 길이와 폭이 각각 6.1, 5.6cm이며, 1개의 꽃대에 20.6개의 소화가 착생하여 꽃 수가 비교적 많이 달리고, 꽃대 길이는 31.8cm 이다. ‘Lovely Angel’는 생육시 잎은 반하수로 자라며 길이는 18.7cm, 폭은 6.8cm이다. 신품종인 ‘Lovely Angel’은 소형 분화용으로 이용 가능할 것이다.

Viral protein 2 (VP2) of porcine parvovirus (PPV) is responsible for inducing neutralizing antibodies in immunized animals. It is the major viral structural protein. In this study, novel subunit vaccines against PPV based on virus-like particles (VLPs) formed from VP2 proteins (PPV 13-7 Korean strain) were expressed in an insect baculovirus cell system and purified using Ni-NTA affinity column chromatography. These VP2 proteins assembled into virus-like particles (VLPs). They showed antigenic properties similar to those of natural PPV. In addition, they showed high hemagglutination (HA) titers (211 for PPV 13-7 Korean strain). This study provides a foundation for the application of the difference immunization of recombinant protein in the diversity of PPV VP2 genes and in vaccination against PPV in the future.

Acinetospora filamentosa and Microspongium stilophorae are reported as new records from South Korea based on morphological and molecular analyses. A. filamentosa is mainly characterized by having the sparsely branched erect filaments, the scattered meristematic zones, forming crampons, plurilocular sporangia on both prostrate filaments and lower part of erect filaments, and spherical to oval unilocular sporangia formed either sessile or with a pedicel. M. stilophorae is an epiphytic thalli mostly on Stilophora sp. It is characterized by prostrate filaments with irregular cells, short erect filaments with short ramuli, phaeophycean hairs, uniseriate plurilocular sporangia on the terminal part of erect filament. Our molecular analyses of rbcL and cox1 genes reveals that A. filamentosa and M. stilophorae are nested within the clades of Acinetospora and Microspongium, respectively.

Feldmannia chitonicola is reported as a new record from Korea based on morphological studies in laboratory-cultured materials and molecular analyses. F. chitonicola is mainly characterized by a small size (1-2 mm), erect filaments mostly unbranched, plurilocular sporangia produced on both prostrate and laterally on the basal part of erect filaments, and 2 types (lanceolate and ovate) of sporangia. In our cultures, sporangia production was slower at 10°C than in 16°C and 20°C. Our molecular analyses of rbcL and cox1 genes supported its independence from other congeners reported for Korea. This is the first report of F. chitonicola for western Pacific.

Bamboo forests are fast-growing, renewable resources, and their carbon sequestration potential has attracted increasing attention. Although bamboo can be used for many purposes, bamboo forests in Korea represent a generally underutilized resource. The main objective here was to perform an assessment of the physical and mechanical characteristics of different species of bamboo found in Korea. The main species of domestic bamboo are Phyllostachys bambusoides, P. pubescens, and P. nigra; we measured the air-dried density for each of the species, with obtained values of 0.89 g/cm2, 0.79 g/cm2, and 0.83 g/cm2, respectively, giving the density order of P. bambusoides > P. pubescens > P. nigra, with P. bambusoides having the highest density. We then measured the compressive strength of each species, which were 802.84 kgf/cm2, 624.69 kgf/cm2, and 743.77 kgf/cm2, respectively, in the order of P. bambusoides > P. pubescens > P. nigra, with P. bambusoides having the highest compressive strength. Volume and maximum load decreased with increasing node height in the three bamboo species, whereas air-dried density and compressive strength increased. Our results thus add to the pool of essential knowledge about Korean bamboo species, and consequently to the development of a potentially valuable domestic resource in Korea.

Insect cuticle consists of numerous structural proteins, which could interact with polysaccharide, chitin, and alter properly mechanical property of the cuticle. Cuticular Protein Analogous to Peritrophins (CPAPs) are characterized by presence of one (CPAP1s) or three (CPAP3s) chitin-binding domain belong to CBM14/ChtBD2 family. In this study, we investigated physiological functions of TcCPAP1-H and TcCPAP3-C in Tribolium castaneum. RNAi for either TcCPAP1-H or TcCPAP3-C at late instar larvae had no effect on larval-pupal molt nor pupal development. However, the resulting pharate adults failed to shed their old pupal cuticle and died entrapped in it without undergoing eclosion. TEM analysis, in addition, revealed disorganized chitinous horizontal laminae and/or vertical pore canals of rigid cuticle from TcCPAP1-H- and TcCPAP3-C-deficient adults. Desiccation-induced death produced by injection of dsTcCPAP1-H into young instar larvae is also discussed.

Insect cuticle tanning (pigmentation and sclerotization) is a complex and vital physiological process that begins with tyrosine and is responsible for production of both melanin- and quinoid-type pigments. In addition, these quinones undergo isomerization to quinone methides and cross-linking reactions with cuticular proteins for cuticle sclerotization. In this study, we studied the functions of TmDDC and TmY-y as well as TmNAT1, TmADC and Tmebony from Tenebrio molitor, which are involved in the tyrosine-derived melanin- and quinoid-type pigment productions, respectively. The temporal and spatial expression patterns of the genes were analyzed by real-time PCR. RNA interference was performed to understand the genetic regulation and molecular mechanism underlying the darkening and hardening of beetle cuticle.

본 연구는 금잔화를 꽃차로 이용하기 위해 열풍 건조 처리 시간에 따른 항산화 물질함량 및 항산화 활성을 분석하고, 최적 처리 시간을 구명하기 위해 수행하였다. 식용꽃 금잔화를 60℃에서 5시간, 10시간, 15시간 열풍건조 한 뒤 Hunter value를 측정하고 95% 에탄올을 용매로 하여 추출하였다. 항산화 물질인 총 폴리페놀, 총 플라보노이드, 총 카로티노이드 함량과 DPPH radical 소거 활성을 통한 항산화 활성, 이를 통한 항산화 물질 함량과 항산화 활성 간의 상관관계를 분석하였다. 처리시간이 증가함에 따라 Hunter value L 값과 +b값이 모두 감소하는 경향을 보였으며, 15시간 처리구에서 다소 많이 감소하였다. 또한, 항산화 물질 함량을 조사한 결과, 총 폴리페놀 함량은 처리 시간에 따른 차이는 없었으나 총 플라보노이드 함량과 총 카로티노이드 함량은 5시간과 10시간 처리시 함량이 다소 높았으며, 처리 시간이 증가함에 따라 감소하여 15시간 처리시 대조구와 차이가 없는 것으로 조사되었다. DPPH radical 소거 활성 분석 결과, 5시간과 10시간 처리시 대조구보다 활성이 높았으며, 총 카로티노이드가 DPPH radical 소거 활성 간의 상관관계가 높은 것으로 나타났다(p≤0.01, r=0.610). 따라서, 열풍건조한 금잔화는 총 카로티노이드가 항산화 활성에 기여하며, 5시간 및 10시간 열풍건조 처리가 금잔화의 항산화 물질 함량 및 활성 증가에 효과적인 것으로 판단되나 식물의 열처리 가공에 있어 오랜 시간은 내부 조직 및 항산화 물질 파괴에 영향을 미칠 수 있기 때문에 금잔화 열풍 건조 처리 시 5시간이 효과적일 것으로 판단된다.