We constructed a standard curve to quantify Listeria monocytogenes in ready-to-eat product, especially sausage samples, using real-time PCR. A standard curve was generated using serially diluted L. monocytogenes cells in distilled water. When cells were artificially inoculated in 10 g of sausage samples in 90㎖ buffered peptone water, the cell concentration of range was approximately 1.0×108 to 100 CFU/㎖. The standard curve of the serially diluted cells was linear for at least seven orders of magnitude from 103 to 109 CFU/㎖ of L. monocytogenes. When cells were diluted in sausages, the linearity range was from 104 to 108 CFU/㎖. The correlation coefficient (R2) of diluted cells was 0.9888 and the slope of the curve was —2.6621. The coefficient and slope of inoculated samples were 0.9916 and —2.747, respectively. The R2 value for serially diluted L. monocytogenes and artificially contaminated sausage samples were acceptable. The approach described in this study represents the potency of the quantification of L. monocytogenes in sausage samples by quantitative real-time PCR. It can be used in monitoring the presence and persistence of this pathogen in sausages.

본 논문은 사진이나 그림을 중첩을 통하여 실시간으로 포토 콜라주를 생성하는 방법을 제안한다. 사용될 영상들을 포토 콜라주 생성에 용이한 상태로 변형하는 과정을 거쳐 데이터베이스를 생성한다. 생성된 데이터베이스에 영상을 기준으로 위치시키기 가장 적절한 위치에 붙인다. 그리고 이것을 반복하여 실시간으로 포토 콜라주가 생성시킨다. 영상을 붙이는 과정은 각각의 영상을 서로 중첩을 통하여 붙여지는데 이 영상들을 지속적으로 갱신하여 명확하게 표현된 포토 콜라주 결과를 보여준다.

In the environment of 450mm wafers production known as the next-generation semiconductor production process, one of the most significant features is the full automation over the whole manufacturing processes involved. The full automation system for 450mm

본 논문에서는 유체 시뮬레이션을 이용한 후광 효과(halo)를 제안한다. 후광 효과는 주로 캐릭터의 강조를 목적으로 사용된다. 제안 방법은 기존의 2차원 이미지 편집 도구를 이용한 후광 효과 제작 방식과 유사하다. 그러나 2차원 이미 지에 가우시안 블러 효과를 적용하는 방식이 아닌, 3차원 캐릭터의 외곽선을 유체 방정식을 이용하여 확산시킨다는 점에서 차별성을 갖는다. 즉, 후광 효과에 유체와 같은 움직임의 부여가 가능하다. 실시간 계산 효율성을 위해서 3차 원 형상에서 실루엣 외곽선을 검출한 후 평면에 투영하고 2차원 유체 방정식을 적용하는 간접적인 방식을 취한다. 2 차원 결과 영상에 오목렌즈와 같은 이펙트를 추가하여 왜곡과 과장에 의한 입체감 있는 후광 효과가 가능하다. 제안 하는 방법은 매 프레임마다 캐릭터의 외곽선으로부터 새로운 후광 효과를 생성하기 때문에 게임과 같은 실시간 애니 메이션에 더욱 효과적으로 응용될 수 있다.

본 논문에서는 굴절(refractive) 물체의 광학적 현상들을 실시간으로 표현하는 GPU(Graphics Processing Unit) 기반 렌 더링 알고리즘이 제안되었다. 일반적으로 굴절 물체에 대한 광학적 현상들을 렌더링할 때 포톤 매핑(Photon Mapping) 이 널리 쓰이지만, 포톤 매핑 방법은 계산량이 많기 때문에 게임 등과 같은 실시간 응용분야에는 적합하지 않다. 본 논문에서는 굴절 물체의 앞면, 뒷면의 깊이값의 차이와 굴절 레이(ray)를 이용해 탈출지점을 구하며, 탈출지점의 법선 벡터를 근사하여 빛이 굴절 물체를 통과하면서 발생하는 커스틱스(caustics)현상과 굴절현상을 렌더링한다. 굴절 물체의 반사(reflection) 현상은 프레넬 항(Fresnel term)을 근사(approximation)해서 효율적으로 표현하였다. 또한 HDRI(High Dynamic Range Image)로 환경맵을 구성하여 자연광이 굴절 물체를 통해 굴절되어 보이는 현상을 생성하였다.

Sperm chromatin integrity is essential for successful fertilization and development of an embryo. Reported here is a quantification of DNA fragments which is intimately associated with reproductive potential to provide one of criteria for sperm chromatin integrity. Three sperm populations were considered: CONTROL (no treatment), UV irradiation (48mW/, 1h) and (oxidative stress induced by hydrogen peroxide, 10 mM, 50 mM and 100 mM). DNA fragments in boar sperm were evaluated by using ligation-mediated quantitative real-time polymerase chain reaction (LM-qPCR) assay, which relies on real-time qPCR to provide a measure of blunt 5' phosphorylated double strand breaks in genomic DNA. The results in agarose gel electrophoresis showed no significant DNA fragmentation and no dose-dependent response to . However, the remarkable difference in shape and position was observed in melting curve of LM-qPCR. This result supported that the melting curve analysis of LM-qPCR presented here, could be more sensitive and accurate than previous DNA fragmentation assay method.

There has been a huge progress in semiconductor manufacturing processes such as reduction of the design rule(DR) and development of multi-processes. And, semiconductor industries have steadily extended its business and market share by reducing the design rule(DR) and enlarging the wafer size as well as by resolving many difficult technical problems through various noble approaches in order to reduce the production cost and to improve the yield. In semiconductor manufacturing, there is a significant difference in the number of memory chips produced according to the wafer size, wafer yield, and the level of the design rule even though the same number of wafers were put to the manufacturing process. So, almost all semiconductor manufacturing companies reach the conclusion that the enlarged size of wafer should be adopted in order to enhance the productivity and reduce the production cost. Thus, in this study, we investigate the specifications of the key functions and capabilities of the necessary modules in the yield analysis and improvement system required to acquire the stationary wafer yield with considering the 450mm wafer manufacturing system. Then the results of this research will be helpful for constructing the advanced yield analysis and improvement system called Real-Time Fault Monitoring and Detection (RTFMD) system.

In 450mm wafers production environment for next-generation semiconductor production, one of the most significant features is the adoption of full automation to the whole manufacturing processes involved. The full automation system applied to the whole manufacturing processes will prevent the workers from intervening the manufacturing processes as much as possible and increase the importance of each individual wafer noticeably, and thus require a more robust scheduling system for entire semiconductor manufacturing processes. The scheduling system for 450mm wafers production also should be capable of monitoring the status of each individual wafer and collecting useful fab data in real time. Based on data (or information) gathered in each process, the real-time scheduling system function efficiently to control the whole semiconductor manufacturing processes. In this study, we first carry out preliminary survey on the system requirements and the necessary functions for constructing the real time scheduling system with considering the changes from 300mm wafer production environment to 450mm wafer production environment, and then propose the scheduler system architectures for the cluster-type production processes which are the general in 450mm wafer production environment for the efficient production scheduling. The system architectures proposed in this study involve the modules for real-time scheduling, the modules for supporting the cluster-type production, the modules for finding optimal schedules, and so on. Also, we provide the specifications of the key required functions and capabilities of the modules and the relationship among the modules proposed.　

The infectious pathogens against honeybee (Apis mellifera) comprise a heterogeneous group of bacterial, viral, and fungal organisms including Paenibacillus larvae, Deformed Wing Virus (DWV) and Nosema apis. Many species like Paenibacillus larvae, Deformed Wing Virus (DWV) and Nosema apis have been isolated from a number of different continents, e.g. America, Asia and Europe, indicating its wide spread in whole nature. Little is known about the occurrence and distribution in the environment of these pathogens. For a more rapid, systematic and efficient monitoring of each pathogenic species against honeybee in the environment, PCR-based detection systems were developed that allows species-specific identifications of various pathogenic species with one reaction. These could be achieved by selecting specific primers from conserved regions of each species with speciesspecific DNA sequence variations. For the detection of any already known pathogen, well-developed PCR-detection system allows the specific detection of expected pathogenic species based on its specific nucleic acid sequence. Since each pathogenic species delivers a specific PCR-product of different size, bands can be distinguished very easily by simple gel electrophoresis. After the development of real-time PCR system, PCR-based specific detections of honeybee pathogens were dramatically improved their applications, from just detection to quantification of pathogens. These systems, quantitative PCR (qPCR) for the detection of honeybee pathogens, could be distinguished from previous PCR detection on the points of “real-time”, “easy” and “quantitative”. Moreover, very rapid PCR, so-called “Ultra-Rapid Real-Time PCR” were developed recently in field of pathogen-detection. Typical Honeybee pathogens such as Paenibacillus larvae, Israelli acute paralysis virus (IAPV) were successfully detected inner 7 minutes using 30 cycled Ultrarapid PCR. According to development of more rapid apparatus, even 30 cycled, 1 minute PCR seems to be possible. Ultla-Rapid PCR was currently attempted to apply for the direct detection system of all viral pathogens against honeybee from bee-samples and different environmental probes.

Sex-sorting of sperm is an assisted reproductive technology (ART) used by the livestock industry for the mass production of animals of a desired sex. The standard method for sorting sperm is the detection of DNA content differences between X and Y chromosome-bearing sperm by flow cytometry. However, this method has variable efficiency and therefore requires verification by a second method. We have developed a sex determination method based on quantitative real-time polymerase chain reaction (qPCR) of the porcine amelogenin (AMEL) gene. The AMEL gene is present on both the X and the Y chromosome, but the length and sequence of its noncoding regions differ between the X and Y chromosomes. By measuring the threshold cycle (Ct) of qPCR, we were able to calculate the relative frequency of X chromosome. Two sets of AMEL primers were used in these studies. One set (AME) targeted AMEL gene sequences present in both X and Y chromosome, but produced PCR products of different lengths for each chromosome. The other set (AXR) bound to AMEL gene sequences present on the X chromosome but absent esholthe Y-chromosome. Relative product levels were calculated by normalizing the AXR fluorescence to the AME fluorescence. The AMEL method accurately predicted the sex ratios of boar sperm, demonstrating that it has potential value as a sex determination method.

현재 대부분의 교육용 콘텐츠는 학습자들에게 많은 배움의 기회를 제공하고 있음에도 불구하고 그 내용과 형식면에서 필연적으로 가질 수밖에 없는 한계와 문제점으로 인하여, 이를 대체하는 새로운 차세대 교육콘텐츠의 제작 방법에 관한 연구가 요구되고 있다. 제안하는 시스템은 3D MMOG(Massively Multiplayer Online Game)기반의 실시간 대화형 원격강의 시스템으로써 교수 학습자간의 협동문제 해결 및 커뮤니티 강화를 위한 양방향 교육 콘텐츠 시스템이다. 이에 본 논문에서는 제안하는 시스템의 설계 개발을 통해 차세대 원격 교육 콘텐츠의 방향성을 제시 하고자 한다.

The process of berthing/deberthing operations for entering/leaving vessels in Busan northern harbor was analyzed and evaluated by using an integrated VTS(vessel traffic service) system installed in the ship training center of Pukyong National University, Busan, Korea. The integrated VTS system used in this study was consisted of ARPA radar, ECDIS(electronic chart display and information system), backup(recording) system, CCTV(closed-circuit television) camera system, gyro-compass, differential GPS receiver, anemometer, AIS(automatic identification system), VHF(very high frequency) communication system, etc. The network of these systems was designed to communicate with each other automatically and to exchange the critical information about the course, speed, position and intended routes of other traffic vessels in the navigational channel and Busan northern harbor. To evaluate quantitatively the overall dynamic situation such as maneuvering motions for target vessel and its tugboats while in transit to and from the berth structure inside a harbor, all traffic information in Busan northern harbor was automatically acquired, displayed, evaluated and recorded. The results obtained in this study suggest that the real-time tracking information of traffic vessels acquired by using an integrated VTS system can be used as a useful reference data in evaluating and analyzing exactly the dynamic situation such as the collision between ship and berth structure, in the process of berthing/deberthing operations for entering/leaving vessels in the confined waters and harbor.

본 연구에서는 자동기상관측시스템(AWS)을 이용하여 기상요소의 관측 자료를 수집하고 실시간으로 그 자료를 제공하는 웹 페이지를 개발하였다. 이 시스템은 실시간으로 자료를 제공하면서 동시에 데이터베이스(DB)로 누적하여, 사용자의 요청에 따라 과거의 기상 자료를 파일로 제공하는 기능도 있다. 완성된 페이지를 이용하여 학교 현장에서 지구과학 교과의 기상분야 탐구학습에 성공적으로 활용하였다. 이 연구 결과, 기상 관측 자료를 실시간으로 제공함으로써 기상 분야 탐구학습의 현장감을 높일 수 있게 되었다. 또 누적된 과거 기상 자료를 이용함으로써 시간규모가 너무 길어서 실질적인 탐구학습이 어렵던 지구과학 교과의 제약을 일부 극복하게 되었다.

온라인 게임에 대한 수요가 증가하면서 많은 사용자들은 다양한 무선 접속 기술들을 이용하여 장소에 구애받지 않고 게임 컨텐츠를 이용할 수 있게 되었다. 모바일 기반에서 온라인 게임 컨텐츠 및 시나리오를 신속하게 내려받기 위해서는 무선링크를 통한 전송속도가 충분히 확보되어야 한다. 본 논문에서는 이동/무선 환경에서 고속의 데이터 전송을 지원하는 대표적인 3세대 이동통신 표준인 WCDMA/HSDPA 시스템의 기본적인 개념과 핵심 요소들을 소개하고 온라인 게임의 향후 발전 방향을 전망한다.

Inositol 1,4,5-trisphosphate (IP₃) plays an important role in the release of Cα²+ from intracellular stores into the cytoplasm in a variety of cell types. IP₃ translocation dynamics have been studied in response to many types of cell signals. However, the dynamics of cytosolic IP₃ in salivary acinar cells are unclear. A green fluorescent protein (GFP)-tagged pleckstrin homology domain (PHD) was constructed and introduced into a phospholipase C δ1 (PLC δ1) transgenic mouse, and then the salivary acinar cells were isolated. GFP-PHD was heterogeneously localized at the plasma membrane and intracellular organelles in submandibular gland and parotid gland cells. Application of trypsin, a G protein-coupled receptor activator, to the two types of cells caused an increase in GFP fluorescence in the cell cytoplasm. The observed time course of trypsin-evoked IP₃movement in acinar cells was independent of cell polarity, and the fluorescent label showed an immediate increase throughout the cells. These results suggest that GFP-PHD in many tissues of transgenic mice, including non-cultured primary cells, can be used as a model for examination of IP₃intracellular dynamics.

This paper proposes a methodology to measure the risk level in real-time for Business Activity Monitoring (BAM). A decision-tree methodology was employed to analyze the effect of process attributes on the result of the process execution. In the course of