Recently, the importance of on-site detection of pathogens has drawn attention in the field of molecular diagnostics. Unlike in a laboratory environment, on-site detection of pathogens is performed under limited resources. In this study, we tried to optimize the experimental conditions for on-site detection of pathogens using a combination of ultra-fast convection polymerase chain reaction (cPCR), which does not require regular electricity, and nucleic acid lateral flow (NALF) immunoassay. Salmonella species was used as the model pathogen. DNA was amplified within 21 minutes (equivalent to 30 cycles of polymerase chain reaction) using ultra-fast cPCR, and the amplified DNA was detected within approximately 5 minutes using NALF immunoassay with nucleic acid detection (NAD) cassettes. In order to avoid false-positive results with NAD cassettes, we reduced the primer concentration or ultra-fast cPCR run time. For singleplex ultra-fast cPCR, the primer concentration needed to be lowered to 3 μM or the run time needed to be reduced to 14 minutes. For duplex ultra-fast cPCR, 2 μM of each primer set needed to be used or the run time needed to be reduced to 14 minutes. Under the conditions optimized in this study, the combination of ultra-fast cPCR and NALF immunoassay can be applied to on-site detection of pathogens. The combination can be easily applied to the detection of oral pathogens.

In the modern industrial period, the introduction of mass production was most important progress in civilization. Die-casting process is one of main methods for mass production in the modern industry. The aluminum die-casting in the mold filling process is very complicated where flow momentum is the high velocity of the liquid metal. Actually, it is almost impossible in complex parts exactly to figure the mold filling performance out with the experimental knowledge. The aluminum die-castings are important processes in the automotive industry to produce the lightweight automobile bodies. Due to this condition, the simulation is going to be more critical role in the design procedure. Simulation can give the best solution of a casting system and also enhance the casting quality. The cost and time savings of the casting layout design are the most advantage of Computer Aided Engineering (CAE).. Generally, the relations of casting conditions such as injection system, gate system, and cooling system should be considered when designing the casting layout. Due to the various relative matters of the above conditions, product defects such as defect extent and location are significantly difference. In this research by using the simulation software (AnyCasting), CAE simulation was conducted with three layout designs to find out the best alternative for the casting layout design of an automotive Oil Pan_BJ3E. In order to apply the simulation results into the production die-casting mold, they were analyzed and compared carefully. Internal porosities which are caused by air entrapments during the filling process were predicted and also the results of three models were compared with the modifications of the gate system and overflows. Internal porosities which are occurred during the solidification process are predicted with the solidification analysis. And also the results of the modified gate system are compared.

Effects of multi-walled carbon nanotube (MWCNT) type and flow type (shear and elongational flow) on the electrical conductivity of polycarbonate (PC)/MWCNT nanocomposites were investigated. Two different MWCNTs produced a huge difference in electrical conductivity in an injection molded PC/MWCNT nanocomposite. It was observed that MWCNTs having a higher aspect ratio provide much lower electrical conductivity in injection molded PC/MWCNT nanocomposites while the conductivities of compression molded samples from two different MWCNTs were the same. We found that this is due to a difference in the deformability of the two MWCNTs. Nanocomposite samples prepared at a higher extensional rate and shear rate showed lower electrical conductivity. This is attributed to flow induced orientation of the MWCNTs. The experimental results were discussed in relation to variation in the tube–tube contact due to the change of the MWCNT orientation.

This study was successfully achieved out the optimized system for dispersing and mixing condition of resin liquid. The flow analysis was simulated according to the shape of the impeller and the stirring tank using ANSYS software and optimized in advanced design. As a result, it was confirmed that the shape and double number of the impeller on the flow field are influenced better effects in a range of mixing and diffusion areas comparing to single number of impeller. It was considered that stirring was performed more quickly and efficiently under the condition of double numbers and saw tooth type impeller. This result can be applied practically for the mixing tank in the industrial application and avaliable used to make a new system for painting equipment.

In this study, the characteristics of the heat flow on SA(supply air) side of the white smoke reducing heat exchange system according to the change of SA velocity were analyzed in the winter condition (outside temperature 0℃). Also, the mixing process of SA and the EA(exhaust air) is presented in the psychrometric chart to confirm the possibility of reducing white smoke. Solidworks flow simulation was used to analyze the heat flow on the heat exchange system under uniform conditions. As the inflow velocity of SA increased, the temperature of SA decreased due to the convective heat transfer improvement due to the active flow in SA system. And the outlet temperature and absolute humidity of the mixing zone decreased significantly. At SA velocity 7 m/s, the outlet temperature and absolute humidity decreased to about 58% and 82%, respectively.

Recently, copper alloy netting has been proposed as a material for aquaculture facilities that can be set in harsh offshore environments. To design a cage made of copper alloy netting, it is necessary to calculate the flow of water through the netting and force of external sources on the netting. Therefore, this study measured and analyzed the current velocity reduction after passing through the netting and the hydrodynamic forces acting on the netting using copper alloy netting with nine solidity ratios. As a result of the reduction rate of the flow velocity through the netting, the flow reduction rate was increased as the solidity ratio of netting was increased. The flow reduction rate was also increased as the attack angle on the netting was decreased. In analyzing the resistance on the netting, we also discovered that resistance was increased with increase in the flow velocity and solidity ratio. An analysis of the hydrodynamic coefficient acting on the netting is shown that the drag coefficient tends to increase as the attack angle increases. We also analyzed the hydrodynamic coefficient according to the variation of the Reynolds number. When the drag coefficients acting on the netting were analyzed with the different Reynolds numbers, the Reynolds number increased from over 0.3 m/s to a relative constant. Finally, the copper alloy nettings had a smaller velocity reduction rate when comparing the flow velocity reduction rate between copper alloy nettings and nylon nettings.

BSA 용액의 전량 한외여과에서 막모듈의 중력에 대한 경사각(0~180°) 변화에 따라 유발된 자연대류 불안정 흐름 (natural convection instability flow; NCIF)의 막오염 제어 효과를 플럭스 증가 정도로 측정하고 막힘여과 모델로 해석하였다. 막모듈의 경사각이 0°에서 180°로 커질수록 NCIF 유발이 증가하여 막오염 제어 효과가 커져 플럭스가 증가하였다. NCIF의 유발이 가장 큰 경사각 180°에서의 플럭스 값을 NCIF의 유발이 없는 0°에서의 값과 비교한 결과, 2시간의 단기간 운전에서는 플럭스 향상성이 5배, 20시간의 장기간 운전에서는 17배까지 증가하였다. 막힘여과 모델을 적용하여 NCIF의 유발에 따른 플럭스 증가 효과를 해석한 결과, 운전시간 15분 이내에서는 중간막힘 모델 그 이후에는 케이크여과 모델로 해석하는 것이 타당하였다. 막모듈 경사각 180°에서 유발된 NCIF는 15분 이내의 운전 초기에는 중간막힘 오염을 67%까지 감소시키고, 그 이후의 운전 시간에서는 케이크층 오염을 99.9%까지 감소시켰다. 따라서 막모듈에 유발된 NCIF의 주된 막오염 제어 기작은 케이크층 형성을 억제시키는 것이었다.

Using virtual reality technology, users can learn and experience many interactions in virtual space like the actual physical space. This study was conducted to develop air flow simulator that allows farmers and consultants to consult air flow through VR devices by creating a greenhouse or pigpen model. It can help educate farmers about the importance of ventilation effects for agricultural facilities. We proposed CFD visualization system by building a virtual reality environment and constructing database of CFD and structure of agricultural facilities. After consultants can set up situations according to environmental conditions, the users experience the visualized air flow of agricultural facility according to the ventilation effects. Also it can provide a quantified environmental distribution in the agricultural facility. Currently, the CFD data in agricultural facilities are established during winter and summer. In order to experience various environmental conditions in the developed system, The experts need to run CFD data under various environmental conditions and register them in the system requirements.

Severe wall thinning is found on the tube of a low-pressure evaporator(LPEVA) module that is used for a heat recovery steam generator(HRSG) of a district heating system. Since wall thinning can lead to sudden failure or accidents that lead to shutdown of the operation, it is very important to investigate the main mechanism of the wall thinning. In this study, corrosion analysis associated with a typical flow-accelerated corrosion(FAC) is performed using the corroded tube connected to an upper header of the LPEVA. To investigate factors triggering the FAC, the morphology, composition, and phase of the corroded product of the tube are examined using optical microscopy, scanning electron microscopy combined with energy dispersive spectroscopy, and x-ray diffraction. The results show that the thinnest part of the tube is in the region where gas directly contacts, revealing the typical orange peel type of morphology frequently found in the FAC. The discovery of oxide scales containing phosphate indicates that phosphate corrosion is the main mechanism that weakens the stability of the protective magnetite film and the FAC accelerates the corrosion by generating the orange peel type of morphology.

Owing to their scalability, flexible operation, and long cycle life, vanadium redox flow batteries (VRFBs) have gained immense attention over the past few years. However, the VRFBs suffer from significant polarization, which decreases their cell efficiency. The activation polarization occurring during vanadium redox reactions greatly affects the overall performance of VRFBs. Therefore, it is imperative to develop electrodes with numerous catalytic sites and a long cycle life. In this study, we synthesized heteroatom-rich carbon-based freestanding papers (H-CFPs) by a facile dispersion and filtration process. The H-CFPs exhibited high specific surface area (~820 m2 g–1) along with a number of redox-active heteroatoms (such as oxygen and nitrogen) and showed high catalytic activity for vanadium redox reactions. The H-CFP electrodes showed excellent electrochemical performance. They showed low anodic and cathodic peak potential separation (ΔEp) values of ~120 mV (positive electrolyte) and ~124 mV (negative electrolyte) in cyclic voltammetry conducted at a scan rate of 5 mV s–1. Hence, the H-CFP-based VRFBs showed significantly reduced polarization.

잠수함에서 발생하는 수중방사소음은 적함의 소나에 의해 피탐될 확률과 직결되며, 잠수함 저소음화 방안은 생존성 향상을 위해 필수적이다. 최신 잠수함의 경우 기계류 소음저감 및 고속/대형화가 진행됨에 따라 선체 주위에 발생하는 유동소음에 대한 관심이 높아지고 있다. 본 연구에서는 자유수면의 효과를 고려하여 잠수함 형상 주위에 발생하는 유동소음 수준을 예측할 수 있는 소음해석기법을 개발하였다. 잠수함이 자유수면 근처 운항시에 잠수함 주위 유동장의 교란에 의해 발생하는 난류유동소음과 쇄파버블에 의한 소음이 발생한다. 먼저 잠수함 주위 유동장 해석을 위해, VOF법 기반의 비압축성 이상유동(two-phase flow)해석을 수행하여 잠수함 주위 자유수면 형상과 유동장 정보를 도출하였다. 이후 난류유동소음해석을 위해 음향상사기법인 Permeable FW-H를 적용하였고, 쇄파버블 소음해석을 위해 유동해석에서 도출된 난류운동에너지 분포결과를 기반으로 쇄파버블 소음모델을 적용하였다. 최종적으로 개발된 유동소음 해석기법은 선박해양플랜트연구소(KRISO)의 대형캐비테이션터널(LCT)에서 수행된 잠수함 모형 유동소음계측 실험결과와 비교를 통해 검증을 수행하였다.

This paper develops a flow control block for a hydraulic system of a tunnel boring machine. The flow control block is a necessary component to ensure stability in the operation of the hydraulic system. In order to know the pressure distribution of the flow control block, the flow analysis was performed using the ANSYS-CFX. It was confirmed that the pressure and flow rate were normally supplied to the hydraulic system even if one of the four ports of the flow control block was not operated. In order to evaluate the structural stability of the flow control block, structural analysis was performed using the ANSYS WORKBENCH. As a result, the safety factor of the flow control block is 1.54 and the structural stability is secured.

LDV(laser Doppler velocimetry) measurements were conducted on the exit region of the impeller passage and the gap between the impeller and turbine blades under 0.8 speed ratio. The 0.8 speed ratio has an impeller speed of 2000rpm and a turbine speed of 1600rpm. A periodic variation of the mass flow rate is present in many of the measurements made. The frequency of this variation is the same as the frequency of the turbine blades passing the impeller passage exit. It is found that the instantaneous position of the turbine had effect on fluid flow inside the impeller passage and gap region. This study would aid in the construction of higher accuracy CFD models of this complex turbomachinery device.

GM작물은 세계적으로 재배면적이 지속적인 증가되고 있으며, 이로 인한 GM작물의 잠재적인 환경위해성에 대한 우려도 증가되고 있다. 현재까지 국내에서 GM작물의 상업적 재배는 되고 있진 않지만 GM작물의 안전성 평가를 위한 기술 개발은 크게 요구되고 있는 실정이다. 국내외적으로 다양한 GM작물의 유전자이동성 평가가 수행되어왔으나 화분 공급원인 GM 벼의 개화기 차이에 의한 유전자 이동성 연구는 부족한 실정이다. 본 연구에서는 벼의 개화기 차이에 따른 비타민A 강화 벼(PAC)로부터 모품종인 낙동벼와 중만생종인 일미벼, 조생종인 운광벼, 중생종인 대보벼, 통일형인 세계진미벼로의 화분 매개에 의한 유전자 이동성을 평가하였다. 비타민A 강화벼(PAC)의 도입 유전자를 검출하는 PCR 분석을 통해 유전자 이동성 유무를 최종적으로 검증하였다. 총 파종된 종자수에 대한 교잡율은 개화기가 일치하는 낙동벼에서는 0.0007%, 개화기가 차이가 있는 일미벼, 운광벼, 대보벼, 세계진미벼에서는 0%로 나타났으며, 모든 교잡개체들은 비타민A 강화벼(PAC)에 근접한 2m 내에서 발견되었다. 화분 매개에 의한 비타민A 강화벼(PAC)의 유전자 이동 특성은 기존에 연구된 결과들과 비슷한 결과를 보였으며, 벼 재배품종간의 개화기 차이가 화분에 의한 교잡을 결정하는데 중요한 요인으로 작용하였다. 이에 벼 경작지의 기상 조건과 벼 품종간의 개화시기 중복 여부 등이 GM벼에 의한 일반 재배품종 및 잡초성벼로의 유전자 이동 최소화 기술 개발과 안전관리 기준 작성에서 주요 영향 요소들로 고려해야 한다.

PURPOSES : In this paper, the flow of cement paste layers that were contoured at different times were simulated by computational fluid dynamics in 2D axisymmetric conditions to evaluate the effect of yield stress on the flow of cement paste layers. METHODS: In the 2D domain, the cylindrical-shaped bottom layer was placed on even ground and allowed to flow by gravitational force for only 25 s. The exact same shape of cement paste, the top layer, was placed on top of the bottom layer, and both layers were allowed to flow for an additional 25 s. The shape and pressure of both layers were evaluated. RESULTS: The relationship between the yield stress and diameter of the layer was clearly observed from the numerical simulation results. It was also observed that the interface between the top and bottom layers was affected by the yield stress and fluidity of materials. The total pressure underneath the bottom layer can be a good indicator of whether the material is still flowing or not. CONCLUSIONS: The Navier-Stokes equation with Bingham model is an excellent model to simulate the flow of contoured layers, which can be used to explain the flow of materials in various areas such as wet-on-wet pavement construction, structural member precasting, and 3D printing construction.

This study investigates the enhancement of the oxygen diffusion rate in the cathode channel of a proton exchange membrane fuel cell (PEMFC) by pure oscillating flow, which is the same as the mechanism of human breathe. Three-dimensional numerical simulation, which has the full model of the fuel cell including electrochemical reaction, ion and electronic conduction, mass transfer and thermal variation and so on, is performed to show the phenomena in the channel at the case of a steady state. This model could analysis the oscillating flow as a moving mesh calculation coupled with electrochemical reaction on the catalyst layer, however, it needs a lot of calculation time for each case. The two dimensional numerical simulation has carried on for the study of oscillating flow effect in the cathode channel of PEMFC in order to reduce the calculation time. This study shows the diffusion rate of the oxygen increased and the emission rate of the water vapor increased in the channel by oscillating flow without any forced flow.