Transgenic plants that over express virus coat protein genes have attracted particular interest from researchers, by virtue of their tolerance to virus infection. The transgenic watermelon rootstock analyzed in this study was established by introducing CGMMV coat protein (cp) under the control of CaMV 35S promoter and NOS terminator (Park et al., (2005) Plant Cell Rep. 24: 350-6). The primary objective of this study was to determine the copy number and integration site of the transgene element, in order to develop detection techniques required for monitoring of the transgenic watermelon rootstock. The Southern blot analysis indicated that a single copy of CGMMV-cp gene was inserted into the genome of transgenic watermelon rootstock. We also identified the genomic sequences flanking the integration site of the transgene by inverse PCR analysis. In an effort to find a sequence usable as an internal positive control for the screening of the watermelon and watermelon rootstock, we found that the Sat and DIP-1 genes appears as one copy within their genomes and is watermelon rootstock- and watermelon-specific. The information of the integrated site and the internal positive control sequence was used to establish a new event-specific PCR-based detection method. In addition, mRNA and protein expression level of the transgene in the transgenic watermelon rootstock and grafted watermelon were investigated. The expression of both mRNA and protein of CGMMV-CP was not detected in the transgenic watermelon rootstocks and watermelons, suggesting that the movement of transgene products from transgenic rootstock to watermelon does not occur at our detection level.

This study was carried out to get the genetic information on the germination rate and fat acidity after 12 weeks’storage at 35 . Germination rate decreased with longer storage period, ℃ while fat acidity increased. Germination rate was higher in the order of Koshihikari, Hanmaeum and Unkwangbyeo after 12 weeks’storage at 35℃. Fat acidity of Koshihikari and Hanmaeum was gradually increased, but that of Unkwangbyeo was dramatically increased after 4 weeks’storage at 35℃. The genetic mode on germination rate and fat acidity using the joint scaling test was shown to be additive-dominance gene effects. Additive([d]) effect was higher than dominance effect([h]) in all crosses. The average degree of dominance, [h]/[d], was less than 1, indicating that germination rate and fat acidity was partially dominant. Germination rate and fat acidity based on frequency distribution of 2 crosses were quantitative characters expressed by polygenes.

본 연구에서는 고등해석과 유전자 알고리즘을 이용한 트러스 구조물의 최적설계를 수행하였다. 본 연구에서 사용한 고등해석은 기하학적 비선형과 재료적 비선형을 동시에 고려한다. 최적화 알고리즘으로 마이크로 유전자 알고리즘을 사용하였다. 목적함수로 구조물의 중량을 사용하였으며, 제약조건식은 구조시스템의 하중-저항능력 및 변위 조건을 고려하였다. 제안된 방법에 의한 최적설계 결과를 기존의 연구결과와 비교하여 그 타당성을 증명하였다.

　A total of 41 microsatellite markers were used with 29 genotypes to examine the relationship between SSR polymorphisms and N‐use efficiency related traits with a goal to identify the putative QTLs related to these traits. These primers yielded a total of 183 alleles (average 4.46 alleles per primer), and polymorphism information content (PIC) values of the SSRs ranged from 0.119 to 0.805 with mean value of 0.425. Correlation coefficients were obtained among the four N‐use efficiency traits in the 34 accessions and significant positive correlations of relative ratios between grain yield and harvest index (r=0.3404) and total dry matter (r=0.7976), while N uptake showed a moderate level of correlation with the ratios of the grain yield and total dry matter, respectively. 36.5% (15/41) SSR markers were monomorphic among the 25 japonica accessions out of the 29 accessions. Association between SSR genotypes and phenotypic performances from the total (29) or japonica (25) accessions was tested based on a single point analysis. Three putative QTL regions were detected for the ratio of grain yield. These include the chromosomal region containing the RM283 locus on chromosome 1 and RM25 on chromosome 8 (all and japonica accessions) and the region with the SSR marker, RM206 on chromosome 11 (the japonica accessions). For the total dry matter ratio, two chromosomal regions were identified as the putative QTL region. One is the region with the SSR marker, RM162 on chromosome 6 (all and japonica accessions) and the other was the one with the SSR marker RM25 on chromosome 8 (the japonica accessions). Among these markers, RM25 showed associations with both traits.

고마리의 24개 지역집단으로부터 PCR을 통한 RAPD 분석을 실시하였다. PCR을 통해 증폭된 RAPD절편들은 200-1, 900bp 사이의 구간에서 관찰되었다. 총 16개의 oligoprimer를 이용한 효소중합반응에서 184개의 유효한 polymorphic band markers를 확인하였다. RAPD 분석결과를 기초로 UPGMA 방법에 의한 유집분석을 수행한 결과, 고마리 개체군은 교란형 하천(도시하천, 농촌하천)과 자연적 수환경으로 유집되었고, 교란형 하천 보다 자연적 수환경의 고마리 개체군끼리 유전적 유연관계가 높은 것으로 나타났다. 또한 자연적 수환경과 교란형 하천에 생육하는 고마리 개체군간에 뚜렷한 유전적 한계를 나타내어 이들 사이의 유전적 이질성이 있는 것으로 생각된다.

본 연구는 딸기 품종의 유전적 변이를 DNA 수준에서 비교함으로써 육종의 기초 자료로 활용하기 위하여 딸기 품종 및 계통 50점을 대상으로 AFLP와 SSR 분석을 수행하였다. 총 27종의 AFLP 프라이머와 13종의 SSR 프라이머를 이용여 각각 407개와 42개의 다형성 밴드를 얻었다. 획득된 449개의 다형성 밴드를 이용하여 UPGMA 방식으로 유사도 및 집괴분석을 수행한 결과 유전적 유사도 0.654를 기준으로 6의 그룹으로 분류되었다. 국내 육

의학적 중요성에도 불구하고 다년생 약용식물의 하나인 황기(Astragalus membranaceus)와 관련된 분자육종 연구의 전무한 편이다. 본 연구에서는 황기의 유묘를 대상으로 agroinfiltration법을 이용한 in planta 형질전환을 시도하였다. 형광 및 염색법에 의한 GUS발현 분석에서 황기는 Agrobacterium 감염이 쉽지 않은 것으로 나타났다. 그러나 발아 후 2-3일이 결과한 유묘에 대하여 NaOH나 과산화수소를 적절히 처리

마가목屬 4종류의 엽의 형태적 특성을 분석한 결과 엽형과 엽선 및 엽소질과 같은 정성적 형질은 4종류 모두 피침형 또는 넓은 피침형의 엽형(lanceolate)을 보였으며 엽선은 점첨두(acuminate)로 표면에 털이 없고 밋밋하여 평활(glabrous)한 특성을 보였다. 복엽길이 등 11가지의 정량적 특성 조사 결과는 당마가목 geqq유럽마가목>마가목geqq산마가목 순의 경향으로 종간 유의적 차이를 확인할 수 있었다. 소엽수와 복엽 및 정소엽형지수를

RAPD analysis showed that all the OTUs of 'Sandolbae' were the same species because amplified band patterns of all samples generated by each of 5 random primers were identical. Even though there were different environmental conditions, all the "Chuiangne" trees from three different places were the same species, and also all the "Cheongshilli" trees were the same species too. No genetic variations were detected between native Korean pears grown in the habitats and in the research field. Because 212 polymorphic bands were generated by 9 primers selected through primer screening, they were possible to analyze genetic relationship among naturally growing three native Korean pears and nine cultivars of Pyrus pyrifolia and P. communis. Based on the RAPD analysis, three main groups were formed. The first group represented the Six P. pyrifoia cultivars, the second group was the three native Korean pears, and the last group was the three P. communis cultivars. Genetic distance between 'Wonwhang' and 'Chojuro' was closer than other cultivars in group 1 since dissimilarity index value between these two cultivars was 50.82. However, genetic distance between 'Niitaka' and 'Chojuro' was the most distant compared to the others in group 1. In group 2, 'Sandlobae' was genetically closer to 'Chuiangne' than 'Cheongshilli' because dissimilarity index value between 'Sandlobae' and 'Chuiangne' was smaller, 50.82, than the value between 'Sandlobae' and 'Cheongshilli', 63.636. In group 3, 'Old Home' was genetically closer to 'Bartlett' than 'Kaiser Alexander(or Bosc)'. Group 3 composed of P. communis cultivars was genetically further than other two groups, P. pyrifolia cultivars and native Korean pears.

The genetic diversity was evaluated using RAPD and ISSR among natural populations and Korean wheat cultivars (Triticum aestivum). Understanding the genetic diversity of putative parental and wild stocks would be useful in wheat breeding programs. Ninety three populations were evaluated with fifty RAPD and three ISSR primers. A total of 185 RAPD and ISSR polymorphism were produced. These markers were considered to estimate the genetic distance among accessions. The genetic similarity ranged from 0.41 to 0.86. The dendrogram were constructed by using the UPGMA clustering algorithm based on genetic similarity. The genetic diversity within and among accession was assessed through Principal Component Analysis (PCA) for statistics analysis. In cluster analysis, four groups were clustered and 17 accessions were not clustered. The PCA was corresponded well to the result. This study provides basic information about the genetic relationships for breeding purposes.

This study was conducted to clarify the relation of the species of genus Sorbus in Korea based on multivariate analysis for the morphological characteristics and DNA polymorphism. Twenty-eight quantitative characters were assessed and analyzed by the principal component analysis and UPGMA cluster analysis. From the principal component analysis of 28 quantitative characters, three principal components (PC’s) explained the variation of inter-specific relations among the genus Sorbus. The first PC’s explained 58.95% of the variation with the Eigenvalue of 16.5, and the second and third PC’s showed the Eigenvalue of 8.3 and 3.1 and explained 88.74% and 100.0% of the variation, respectively. Especially, the first PC’s was related in order of the fruit width (FW) and length of terminal leaflet (LTL), petal length (PL), width of terminal leaflet (WTL), and diameter of winter bud (DWB). The second and third PC’s were involved in order of the No. of leaflet (NL), No. of fruit per fruiting lateral (NFL), length of upper rachis (LUR), and diameter of rachis (DR), No. of pistil (NP), respectively. Cluster analysis using them UPGMA method based on the principal components of four species of the genus Sorbus divided into two groups. Group Ⅰ comprises Sorbus commixta and S. sambucifolia var. pseudogracilis, and Group Ⅱ consists of S. amurensis and S. aucuparia. The pattern of DNA polymorphism of the 56 inter-simple sequence repeat (ISSR) markers revealed that different taxa shared different sets of bands, and DNA analysis is useful for taxonomic study on the genus Sorbus.

Demand for high quality rice has always been a major factor in the international rice marketing. In the present study, doubled haploid (DH) population derived from anther culture of a Tongil/japonica hybrid was used for genetic analysis of rice grain quality. The average values of DH lines for grain weight, grain length and the ratio of grain length to width were near the mid-parent value. More than 40% DH lines showed transgressive segregation for grain weight, length, amylose and lipid content, but less than 10% DH lines observed on ratio of length to width and grain thickness were transgressive segregation. Correlation analysis between appearance qualities and physicochemical characters indicated that grain width and grain thickness both significantly and negatively correlated to protein and lipid content. A highly significant negative correlation between protein content and amylose content was observed.