The pattern of wound healing process differs markedly according to the cell types. Gingival wounds heal more rapidly without scar, however dermal wounds show collagen laid down in thick disorganized patterns and keloid formation. This h as b een s uggested t o be d ue t o the presence of d ifferent E C M components a nd c ytokines a s well a s growth factors. The purpose of this study was to examine the differential expression of genes in connection with keloid formation in gingival fibroblasts (hGFs) and dermal fibroblasts (hDFs) in response to inflammation. In this study, we investigated the differences between hGFs and hDFs in the expression and production of cyclooxygenase (COX-2), prostaglandins E2 (PGE2), transforming growth factor (TGF)-β, collagens, matrix metalloproteinases (MMPs), and tissue inhibitors of matrix metalloproteinases (TIMPs) which play important roles in collagen deposition in wound healing. The hGFs and hDFs were primary cultured and allocated to arachidonic acid (AA) treatment group and control group. Protein and mRNA were extracted right after (0 hr) and 24 hr after AA treatment. At a defined concentration of AA in hGFs and hDFs, MTT assay was performed. The mRNA and protein expression levels of COX-2, TGF-β, collagen 1 and 3, MMP 1 and TIMP 1 were examined by Real-time PCR and Western blots. The amounts of PGE2 were measured by enzyme-linked immunosorbent assay (ELISA).The expression of COX-2 and TGF-β exhibited reduced levels in hGFs , but were increased in hDFs at 24 hr after AA treatment. Production of PGE2 was increased in hGFs and hDFs at right after AA treatment but, not changed at 24 hr after AA treatment. The protein and mRNA expression of collagen 1 and 3 were decreased in hGFs , whereas increased in hDFs at 24 hr AA treatment. Expression of MMP-1 protein was increased in hGFs at 24 hr but, was decreased in hDFs at 24 hr compared with that of control. The protein expression of TIMP-1 was decreased in hGFs but, was increased in hDFs at 24 hr compared with that of control. These observations demonstrate differential expression between gingival and dermal fibroblasts in regulation of collagenolytic capacity by extracellular matrix-associated genes in keloid formation associated with wound repair.

Matrix metalloproteinases (MMP) play important roles in extracellular matrix (ECM) remodeling during ovarian follicular development, oocytes development and ovulation. In an attempt to investigate the effect of MMP activation in development cumulus-oocytes complexes, we examined the localization and expression of MMP, and monitored MMP expression profile. Cumulus-oocytes complexes were collected and matured in vitro for 24 hr, 36 hr and 48 hr. A mRNA expression of MMP-2, MMP-9, TIMP-2 and TIMP-3 was detected in all culture medium regardless of CC, OC and COCs. Activity of MMP-2 in the OC progressively was increased from 24 hr to 48 hr. But MMP-9 was not detected in all culture medium. The localization of MMP-2 was also measured by immunohistochemistry analysis. The MMP-2 and TIMP-2 was detected in cumulus cell and oocyte zone pellucida. Expression of MMP-2 protein in the COCs was progressively increased from 24 hr to 48 hr. However, MMP-9 protein was progressively decreased from 24 hr to 48 hr. And TIMP-2 protein was most highly expressed in the COCs 36 hr. Expression of TIMP-3 protein in the COCs was progressively increased from 24 hr to 48 hr. In conclusion, these results suggest that MMP-2 plays a role in maintaining normal maturation and development by controlling the ECM inhibitor concentration on cumulus cell and oocytes.

In this study, we analyzed the effect of silicon oxynitride matrix on the optical properties of Au nanoparticles dispersed on composite film and explored the effectiveness of the silicon in fine tuning the refractive index of the composite film for applications in optical waveguide devices. The atomic fraction of nitrogen in SiOxNy films was controlled by varying the relative flow ratio of nitrogen gas in reactive sputtering and was evaluated optically using an effective medium theory with Bruggeman geometry consisting of a random mixture between SiO2 and Si3N4. The Au nanoparticles were embedded in the SiOxNy matrix by employing the alternating deposition technique and clearly showed an absorption peak due to the excitation of surface plasmon. With increasing nitrogen atomic fraction in the matrix, the surface plasmon resonance wavelength shifted to a longer wavelength (a red-shift) with an enhanced resonance absorption. These characteristics were interpreted using the Maxwell-Garnett effective medium theory. The formation of a guided mode in a slab waveguide consisting of 3 μm thick Au:SiOxNy nanocomposite film was confirmed at the telecommunication wavelength of 1550 nm by prism coupler method and compared with the case of using SiO2 matrix. The use of SiOxNy matrix provides an effective way of controlling the mode confinement while maintaining or even enhancing the surface plasmon resonance properties.

본 실험은 국내 고추종자 12 품종을 대상으로 SMP 처리시 최적의 발아온도와 건조기간을 구명하고, 3 종류의 종자처리기술을 적용하여 고추종자의 입모율과 수확량을 비교하고자 하였다. 발아온도에 따른 결과는 무처리 종자가 15℃에서 12 품종 모두 전혀 발아가 되지 않았다. SMP 처리종자는 발아율이 77(여명)∼100%(대왕)로 높았으며, 15℃ 여명품종의 77%와 35℃의 부강품종 85%를 제외한 나머지 품종은 모든 온도에서 93% 이상으로 높은 경향이었다. 건조조건은 45℃에서 3시간이 적절한 품종으로 금수레, 동방, 종가네, 향촌, 여명, 광복, 왕 품종이었으며, 35℃에서 6 시간이 적절한 품종은 마니따, 조양 품종이었고, 25℃에서 12시간이 적절하다고 판단된 품종은 부강, 다보탑, 대왕 품종이었다. 건조기간에 따른 고추종자의 수분 함수율 변화는 품종마다 다소 차이는 있겠으나, 45℃에서 3∼6시간으로 판단되었다. SMP, osmopriming, pH조절처리에 따른 입묘율을 비교한 결과 낮은 입모율을 보이는 처리는 향촌품종의 pH 조절처리, 다보품종은 무처리, osmopriming 처리였다. 또한 광복품종은 입모율이 낮았고, 대왕품종은 다른 품종에 비해 양호한 경향이었다. 정식 후 90일째 수량에서 무처리가 낮은 경향이었으며, 결주율 또한 높게 나타났다. 품종에 따라 수량의 차이는 있었으며, pH 조절과 osmopriming의 처리 또한 모든 품종에서 안정된 입모율을 보이는 것은 아니었다. 결론적으로 SMP 처리조건은 모든 품종에서 안정된 입모의 확보를 가져올 수 있었으며, 다양한 환경조건에서 반복적인 연구가 진행되어야 할 것으로 사료되었다.

본 연구에서는 국내 주요 고추 12품종의 종자 처리시 적정 SMP carrier 선발을 하고자 하였다. SMP carrier 중 탄산칼슘은 carrier와 수분의 배율이 증가할수록 낮은 발아율을 보이거나, 처리 중 유근이 돌출되어 실용성이 낮은 carrier로 판단되었다. Micro-cel E는 수분의 배율이 증가할수록 T50이 단축되는 경향을 보였으나, 왕 품종의 경우 처리 중 유근이 돌출되어 차후 대량의 종자처리방법으로 적합하지 못한 결과를 나타냈다. 규조토의 처리는 수분의 배율을 1.5배 처리한 조양과 향촌품종이 처리 중 유근이 돌출되는 현상이 발생되기도 하였으나, 수분의 배율이 증가할수록 T50과 MDG가 단축되었다. 왕 품종의 경우 규조토로 1.0, 1.5배 처리는 T50이 0.99일과 0.89일로, Micro-cel E의 5.0, 7.0배의 1.59, 0.94일 보다 0.60일, 0.05일 단축되는 경향이었다. 따라서 고추 품종에 적용 가능한 carrier는 Micro -cel E와 대등한 성능을 갖는 규조토로 판단되며, 수분배율은 1.0배 처리가 적절할 것으로 사료된다.

고추 12 품종의 SMP 처리시 carrier와 수분의 배율, 처리기간 및 처리온도에 대한 조사 결과 적정 처리기간과 carrier와 수분의 배율은 품종마다 차이는 있었지만, 처리 중 발아가 일어나지 않았고, 발아율을 높이며, T50을 단축시킬 수 있는 처리로 왕과 마니따 품종은 carrier와 수분의 배율이 1 : 3에서 7일이었다. 나머지 10 품종은 1 : 7의 비율에서 3일이 좋았다. 종가집 품종은 다른 품종에 비해 처리기간과 수분배율에 따라 발아율과 T50이 불규칙한 경향을 나타낸 품종으로 조사되었다. 처리온도는 15℃에서 35℃로 설정하여 조사한 결과, 처리 중 유근돌출의 억제와 발아성 향상에 안정적인 처리는 20℃인 것으로 조사되었다.

2-dimensional silica-silica Continuous Fiber-reinforced Ceramic.matrix Composites (CFCCs) were fabricated by a sol-gel infilitration method that has a changing processing condition, such as the repetitions of infilitration. In order to investigate the relationship between the processing condition and the mechanical properties of composites, the mechanical properties of specimens were measured by means of a 4-point flexural strength test while the evidence of strength degradation were microstructurally characterized. There seemed to be a minimum density value that existed at which the delamination between the fabrics would not occur. In the case that the density of silica CFCCs exceeded 1.55 g/cm3, the flexural strength also exceeded approximately 18 MPa at least. By applying the Minimum Solid Area (MSA) analysis of the porous structure, the correlation between the relative density and the mechanical properties of composites will be discussed.

This study proposes the generation mechanism of various design matrix using generalized linear model for design of experiment. Design generation method of GLM analysis, factorial design(FD) with center points, ANOVA design with lack-of-fit test, and response surface design are introduced. In central composite(CC) design, orthogonal blocking and fractional factorial design(FFD) are presented. We compare the design of Box-Benhken(BB) and face-centred central compsite design.

Mutations in DLX3 are associated with both autosomal dominant hypoplastic hypomaturation amelogenesis imperfecta (ADHHAI) and tricho-dento-osseous (TDO) syndrome. ADHHAI is caused by a c.561_562delCT (2bp-del DLX3) mutation whereas TDO syndrome is associated with a c.571_574delGGGG (4bp-del DLX3) mutation. However, although the causal relationships between DLX3 and an enamel phenotype have been established, the pathophysiological role of DLX3 mutations in enamel development has not yet been clarified. In our current study, we prepared expression vectors for wild type and deletion mutant DLX3 products (4bp-del DLX3, 2bp-del DLX3) and examined the effects of their overexpression on the expression of the enamel matrix proteins and proteases. Wild type DLX3 enhanced the expression of matrix metalloprotease 20 (MMP20) mRNA and protein in murine ameloblast-like cells. However, neither a 4bp-del nor 2bp-del DLX3 increased MMP20 expression. Wild type DLX3, but not the above DLX3 mutants, also increased the activity of reporters containing 1.5 kb or 0.5 kb of the MMP20 promoter. An examination of protein stability showed that the half-life of wild type DLX3 protein was less than 12 h whilst that of both deletion mutants was longer than 24 h. Endogenous Dlx3 was also found to be continuously expressed during ameloblast differentiation. Since inactivating mutations in the gene encoding MMP20 are associated with amelogenesis imperfecta, the inability of 4bp-del or 2bp-del DLX3 to induce MMP20 expression suggests a possible involvement of such mutations in the enamel phenotype associated with TDO syndrome or ADHHAI.

Gingival overgrowth can cause dental occlusion and seriously interfere with mastication, speech, and dental hygiene. It is observed in 25 to 81% of renal transplant patients treated with cyclosporine A (CsA). CsA-induced gingival overgrowth (CIGO) is caused by quantitative alteration of the extracellular matrix components, particularly collagen. However, the molecular mechanisms involved in the pathogenesis of CIGO remain poorly understood, despite intense clinical and laboratory investigations. The aim of the present work is to identify differentially expressed genes closely associated with CIGO. Human gingival fibroblasts were isolated by primary explant culture of gingival tissues from five healthy subjects (HGFs) and two patients with the CIGO (CIGO-HGFs). The proliferative activity of CsA-treated HGFs and CIGO-HGFs was examined using the MTT assay. The identification of differentially expressed genes in CsA-treated CIGO-HGF was performed by differential display reverse transcriptase-polymerase chain reaction (RT-PCR) followed by DNA sequencing. CsA significantly increased the proliferation of two HGFs and two CIGO-HGFs, whereas three HGFs were not affected. Seven genes, including the beta subunit of prolyl 4-hydroxylase (P4HB) and testican 1, were upregulated by CsA in a highly proliferative CIGO-HGF. The increased P4HB and testican-1 mRNA levels were confirmed in CsA-treated CIGO-HGFs by semiquantitative RT-PCR. Furthermore, CsA increased type I collagen mRNA levels and suppressed MMP-2 mRNA levels, which are regulated by P4HB and testican-1, respectively. These results suggest that CsA may induce gingival overgrowth through the upregulation of P4HB and testican-1, resulting in the accumulation of extracellular matrix components.

Thrombospondins (TSP-1, TSP-2) are secretory extracellular glycoproteins that are involved in a variety of physiological processes such as tumor cell adhesion, invasion, and metastasis. The present study was undertaken to elucidate the involvement of thrombospondins in the adhesion of osteoblast-like cells using the TSP-1 or TSP-2 antisense MG63 and MC3T3-E1 cell lines. For downregulation of TSPs expression, we prepared antisense constructs for TSP-1 and TSP-2 using the pREP4 an episomal mammalian expression vector, which be able to produce the specific antisense oligonucleotides around chromosome. MG63 and MC3T3-E1 osteoblast-like cells were transfected with the antisense constructs and nonliposomal Fugene 6, and then selected under hygromycin B (50 μM/mℓ) treatment for 2 weeks. Western blot analysis revealed that expression of the TSP proteins was downregulated in the antisense cell lines. The cell adhesion assay showed that adhesive properties of TSP-1 and TSP-2 antisense MG63 cells on the polystyrene culture plate were reduced to 17% and 21% of the control cells, respectively, and those of the TSP-1 and TSP-2 antisense MC3T3-E1 cells also decreased to 19% and 27% of control, respectively. Adhesion of TSP-1 and TSP-2 antisense MC3T3-E1 cells on Type I collagen-coated culture plate decreased to 27% and 76%, respectively. These results indicate that TSP-1 and TSP-2 proteins may have an important role in adhesion of osteoblast-like cells to extracellular matrix.

This paper reports generating creative ideas based on customer needs using Kano Model and Importance-Differentiation Matrix (I-D Matrix). Nowadays, every customer demands creative ideas on product innovations in order to be satisfied her needs. However, most existing methods are limited to get creative ideas that reflect customer needs. Any creative ideas that do not fully reflect customer needs are obviously more difficult to succeed in the market than those that reflect customer needs. This paper distinguishes each quality elements the customer needs in terms of Kano Model. And it presents the effective ways of generating creative ideas by I-D Matrix in order to overcome current uppermost limits.

본 연구는 ‘기술/의지 매트릭스’에 따라 코칭의 적용 방법이 달라지는 것에 착안하여 ‘기술’과 ‘의지’의 용어를 이해하고, ‘기술’과 ‘의지’의 정도에 따라 적용하는 코칭 방법 그리고 ‘기술/의지 매트릭스’에 따른 코칭 적용의 효과와 한계를 탐색하였다. 매트릭스는 수학, 생물학, 공학, 경영학 등에서 사용되는 데, 문헌 연구 결과 수학을 근원으로 결론지었다. ‘기술’은 인간의 경험지식을 의미하는 단어이며, ‘의지’는 인간의 심리적 결단을 의미하는 단어이다. ‘기술’과 ‘의지’에 따라 Pull과 Push의 코칭 방법이 배분되어 사용되는데, Pull는 코치이에게 판단과 행동의 주도권을 주는 방법이며, Push는 코치의 판단과 충고가 작용하는 방법이다. ‘기술/의지 매트릭스’는 분석철학과 실용주의 철학에 연결되어 있으며, 리더십 이론 중에서 상황적합성 이론과 허시(Hewsey)와 블랜차드(Blanchard)의 수명주기 이론을 배경으로 둔다. 코치이의 ‘의지’와 ‘기술’이 모두 낮은 경우 ‘지시’의 리더십과 코칭이 사용되고, ‘의지’는 높으나 ‘기술’이 낮으면 ‘지도’의 리더십과 코칭이 사용된다. ‘기술’은 높으나 ‘의지’가 낮으면 ‘격려’의 리더십과 코칭이 사용되며, ‘의지’와 ‘기술’이 모두 높은 경우 ‘위임’의 리더십과 코칭이 사용된다. 네 가지 경우 모두 Pull과 Push의 코칭 방법이 적절하게 사용된다. ‘기술/의지 매트릭스’가 코칭에 사용될 때는 첫째, 코치이의 ‘의지’와 ‘기술’을 진단하고, 둘째, 진단결과에 따라 코칭 방법을 결정하며 셋째, 코치와 코치이의 합의에 의해 실행하는 것이다. ‘기술/의지 매트릭스’는 상황에 따라 융통성을 발휘하며 코칭하기에 적절하지만 명확한 진단도구가 없으며, 코치이의 의도적인 거짓말을 막기 어렵다. 그러므로 코치이를 잘 아는 코치가 사용하기에 적합한데 기업 내에서 상사가 부하 직원을 코칭할 때 적합하다.

Deraeocoris brevis Knight (Hemiptera: Miridae) is a generalist predator and is a key natural enemy in pear orchards in the northwestern United States. Although D. brevis undoubtedly contributes to the regulation of major pear pests, pesticides often disrupt its activity and reduce its effectiveness as a biological control agent. A temperature-dependent stage-structured matrix model was developed to analyse the population dynamics of D. brevis influenced by insecticides. In this study, impacts of acetamiprid on field populations of D. brevis were analysed. The age class of D. brevis was categorized into four stages: eggs, small nymphs (1st to 3rd instar), large nymphs (4th to 5th instar), and adults. Probabilities for each element in the projection matrix were estimated using published temperature-dependent developmental data of D. brevis. Transition probabilities from an age class to the next age class or the probabilities of remaining in the age class were obtained from development rate function of each stage (age classes). The fecundity coefficients of adult population were the products of adult longevity completion rate (1/longevity) and temperature-dependent total fecundity. Also, direct and indirect residual effects of acetamiprid were incorporated into the model. The model results were much overestimated compared with observed actual data from 25d after model running. Such a discrepancy might be occurred from various reasons such as an intra-species competition, successful fecundity rate, etc. Further, the improvement and application of the model were discussed.

전단빌딩에 발생한 손상 추정에 있어서 대상 구조물의 물성치를 가정하고 이상화한 모델을 이용한 역해석이 필요하다. 강성행렬을 이용하는 고전적인 손상추정 방법에 비해 유연도 행렬을 이용한 손상추정은 구조물의 저차모드를 이용하기 때문에 비교적 정확한 값을 계산할 수 있기 때문에 더 효과적으로 알려져 있다. 이 논문에서는 손상추정을 위한 알고리즘으로 유전자 알고리즘(Genetic Algorithm, GA)을 도입하였고, 구조 응답에서 취득할 수 있는 유연도 행렬을 이용하여 역해석을 통한 손상추정 기법을 소개하고 있다. 제안된 손상추정 기법은 전단빌딩의 강성에 대한 정확한 정보가 없는 상황에서 전단빌딩의 손상으로 인한 실제 강성변화량을 추정하도록 하였다. 더불어 open source code인 OPENSEES를 이용하여 전단빌딩 수치해석을 통해 제안된 손상추정 기법의 효율성을 검증하였다.

Human gingival fibroblasts are necessary for oral homeostaslS These cells are fundamental in tissue healing and tissuc remodeling processes under a response to physiological actions such as mastication, Collagen and elastin, that are extracell ular glycoprotein of gingival fibroblast, are found in all animals, '1γpe 1 collagen is most dominant protein found in human gingival fibroblasts , Matrix metalloproteinase-1(MMP-1) has a role play in destruction of metabolism of extracellular matrix(ECM) and MMP-1 can destroy many ECMs as well as non-ECM molecules MMP-1’s local activation is conytolled by tissue inhibitor of metalloproteinase-1(TIMP-1) , Therefore, it is important to have a balance between in both s ituations MMPs and TIMPs of increased 0 1' decreased extracelluar matrix molecules, The purpose of trus study is to find out the effect of physical stimulus to human gingival fibroblast on mRNA, proteins of collagen 1, elastin, MMP-1, and TIMP-1 Healthy human gingival fibroblasts were separated and cultur때 in DMEM(Dulbeco’s Modified Eagle’s Medium) , When the sample reached to confluence state, it was separated with 0,25% t rypsin and 0‘ 53mM ethylendiaminetetraacetic aCld Separated cells were centrifuged in a cell culturing flask at 1000rpm for 30, 60, 120 mmutes Then it was forced by 35g/cm' continuously, The obtained results that expression of mRNA using histological study and Reverse Transcription Polymerase Chain Reaction (RT-PCR) , expression of protein using Enzyme-Linked Immunosorbent Assay(ELISA) for this study, At 30minutes after cen trifuging, there were s pindl e shaped gingival fibroblasts with long processes parallel to other cells in the control group , However the cell density was simil ar to compared group, At 60minutes after centrifuging, spindle shaped human gingival fibroblast with relatively long process, less densely packed, At 120minutes after centrifuging, cell processes were lengthened 2-3 times‘ and cell density was lower, At 30-60 minutes after centrifuging, it was increased by 1,3-1,7 times in expressoin of collagen 1 mRNA as compared with comparison group, However, there was no change in elastin, TIMP-l, and MMP-1, At 120 minutes after centrifuging, The revealed collagen 1 mRNA was increased 3 times as compared with comparison group, It was increased 2 times in elastin , 12 times in TIMP-1 as compared with comparison group, However, there was no change in MMP-l. At 30-60 minutes after centrifuging, it was increased by 1.1 times in revealing of protein revealing in collagen 1, TIMP-1 But there was no cha nge in elastin, MMP-1 At 120 minutes after centrifuging, it was increased by 1,2 times in revealing collagen 1 protein, 11 times in elastin, 12 times in TIMP-l, but there was no change in MMP-l. ln conclu s ion, it increased in revelation of collagen 1 ,elastin and TIMP-1 by continous stimulus in human gi ngival fibroblast, But there was no change in revelation of MMP-l Therefore, th is type of pressu re is one of the components for healing of gingiva l fibroblast

This paper discusses a scheduling problem on object-oriented developments over multiple teams with limited resources in matrix organization. The objective of the problem is to minimize the makespan of overall projects. There are tangible and intangible advantages such as efficient resource share, improvement of productivity, development efforts and cost reduction, etc. by dispatching resources properly to the development teams. Traditionally, the project scheduling has been done with a manager's intuition or heuristic. We present a scheduling model with illustrative examples, stochastic search approach, and apply a variety of problems generated randomly to the approach. The results are analysed.