When fully grown oocytes are removed from their follicles, they can resume meiosis and mature spontaneously under in vitro conditions. However, nuclear maturation under in vitro condition is not accompanied by complete cytoplasmic maturation, which is essential for successful fertilization and the initiation of zygotic development. This study analyzed change of proteins in follicular fluids during the porcine follicular development. Follicular fluids were collected from follicles of diameter 1～2 mm, 2～6 mm and 6～10 mm in ovary of slaughtered pigs. Total proteins were extracted from follicular fluids by M-PER Mammalian Protein Extraction Reagent. We confirmed totally 27 same spots, 1 spot from follicle fluid of 2～6 mm follicle and 5 spots from follicle fluid of 6～10 mm in diameter were analyzed by MALDI mass spectrometry and searched on NCBInr. In results, spot No. 28 from 2～6 mm follicle was Ig lambda chain C region, and spot No.32 and 33 from 6～10 mm was Apolipoprotein A-(APOA4). Spot No.29 and 31 were failed to analyze. These results indicate that the porcine oocyte during in vitro maturation depend on specific different expressed proteins may play an important roles in the sequence of molecular events in porcine oocyte maturation and follicular development.

The purpose of this study was to determine the relationship between conception rate and other parameters (body condition score; BCS, progesterone concentrations and follicle size) before estrus induction with . The conception rate in cows with (2.75, 2.75 to 3.25 and 3.25), BCS regardless of AI (artificial insemination) time was 47.5, 67.5% and 48.5% at injection, respectively. The conception rate regardless of BCS was 59.0% in cows inseminated based on detected estrus, and 46.2% in cows inseminated at 72 to 80 hours (timed artificial insemination, TAI) after injection. The conception rate regardless of AI time was 43.0% in cows with low progesterone concentrations (less than 1.0 ng/ml), and 67.5% in cows with high progesterone concentrations (more than 1.0 ng/ml) at injection. The conception rate regardless of progesterone concentrations was 59.9% in cows inseminated based on detected estrus, and 48.1% in cows of TAI after injection. The conception rate regardless of AI time was 36.0% in cows with small dominant follicles (less than 5 mm), 56.0% in cows between 5 mm to 10 mm of follicle size, and 65.5% in cows with large dominant follicles (more than 10 mm) at injection, respectively. The conception rate regardless of follicle size was 57.3% in cows inseminated based on detected estrus, and 47.6% in cows of TAI after injection. These results indicated that if the cows with BCS 2.75 to 3.25, active corpus luteum, and/or large dominant follicle (more than 10 mm) are used for estrus induction, the conception rate will be greater.

자생 초본식물의 보존과 임간재배에 필요한 기초자료를 얻고자, 가리왕산과 중왕산 지역의 아고산대에 분포하는 천연활엽수림을 중심으로 주요 자생 초본식물의 분포와 입지인자를 조사하고 상호관련성을 분석하였다. 초본식물 분포조사에서는 136종의 초본식물이 확인되었으며, 개별꽃의 상대우점치가 10.8%로 가장 높게 나타났다. 그 밖에 주요 식물종은 벌깨덩굴, 현호색, 참나물, 대사초, 단풍취, 큰개별꽃, 얼레지, 곰취, 만주족도리풀, 피나물, 노루오줌 등 이었다. 노루귀와 단풍취; 참나물과 두메투구꽃; 두메투구꽃과 풀솜대; 벌깨덩굴과 큰개별꽃, 등의 종간에는 매우 높은 정의 상관이 인정되었다. 주요 식물종의 유집분석 결과, 7개의 그룹으로 나뉘어졌다. 주요 자원식물종이 선호하는 입지인자들이 밝혀졌다. 곰취, 단풍취, 박쥐나물, 얼레지, 참나물 등은 해발고가 높을수록 많이 분포하였고, 곰취는 토심이 깊을수록 많이 분포하는 것으로 나타났다. 얻어진 결과는 평창군 아고산 천연활엽수림의 보전과 주요 지원식물의 임간재배에 활용할 수 있을 것이라 사료된다.

To research indoor air pollution in the training rooms of technical high school, the temperature, air current, humidity, CO₂, CO, O₃ were measured by Indoor Climate System(ICS 500, Casella, UK) at each classroom, scientific laboratory, electricity training room, electronic equipment training room and welding training room. The change of air pollution concentration and its correlation were additionally compared and analyzed. At closed small space such as classroom, scientific laboratory, electronic equipment training room and electricity training room, the CO₂ concentration was obtained to the 2,030ppm(max.), which is higher than notified and recommended standard value(1,000ppm) by Ministry of Health and Welfare and Ministry of Environment, Korea. At welding training room where is larger and more ventilated than general classroom, CO concentration was measured to the 3.6ppm, which is higher than average 1ppm measured at other training rooms. The concentration of O₃ is not yet regulated from the standards of underground air quality, but at welding training room it was measured as 0.11ppm(max.) that is higher than 0.01ppm measured at other training rooms. The higher value of temperature, air current, radiant temperature and CO₂ concentration was shown at scientific laboratory, electricity training room and electronic equipment training room where are closed and same with the scale of classroom. And the higher concentration of CO₂, CO and O₃ was shown at welding training room which was opened larger classroom. The indoor air pollution by CO₂, CO and O₃ may directly affect on the training room where many students work at a small space, and they should be controlled appropriately. Each experimental formulas were made for the estimation of CO₂, CO and O₃ concentration depending on some kinds of variables at each training room. It is found that indirect ventilation system with a filter will be needed for regular and constant ventilation and the ventilation system should be applied to protect and make clean and comfortable environment of training rooms at technical high school.

The paper reviews gauge R&R studies by two-factor mixed models including random and fixed factors. The two-factor mixed models include restricted models and unrestricted models considering the interaction of two factors. This study also classifies the models according to the number of factors, and the combination of various factors such as random factor, fixed factor, block factor and repetition type.

Semiconductor industry is based on equipment industry and timing industry. In particular, semiconductor process is very complex and as semiconductor-chip width tails and is becoming equipment gradually more as a high technology. Equipment operation is primarily engaged in semiconductor manufacturing (engineers and operator) of being conducted by, equipment errors have also been raised. Equipment operational data related to the error of korea occupational safety and health agency were based on data and production engineers involved in the operator's questionnaire was drawn through the error factor. Equipment operating in the error factor of 9 big item and 36 detail item detailed argument based on the errors down, and 9 big item the equipment during operation of the correlation error factor was conducted. Each of the significance level was correlated with the tabulation and analysis. Using the maximum correlation coefficient, the correlation between the error factors to derive the relationship between factors were analyzed. Facility operating with the analysis of error factors (big and detail item) derive a relationship between the model saw. The end of the operation of the facility in operation on the part of the two factors appeared as prevention. Safety aspects and ergonomics aspects of the approach should be guided to the conclusion.

Adherent cells, such as those found in epithelial tissues, must be physically associated with extracellular matrix (ECM)components to survive. Though stimulation by growth factors is an essential factor in cell survival, normal cells also requires cell adhesion to ECM proteins. The cessation of these anchorage-mediated signals seems to be a common mechanism to physiolog ically t erminate t he l ife cycle of t hese c ells b y apoptosis. This form o f cell death has been termed anoikis.In cancer, resistance to anoikis of cancer cell is important in invasion and metastasis. The present study investigated the intracellular mechanism involved in anoikis, especially in cells treated with epigallocatechin- 3-gallate(EGCG). To induce anoikis, cell culture plates were coated with 10 ㎍/ml poly-HEMA. A549 lung adenocarcinoma cells were grown in RPMI 1640 medium with/without 10% fetal bovine serum, and then cells were replated on cell culture dishes coated with poly-HEMA in the presence or absence of serum. On the other hand, EGFR inhibitor, PI3K inhibitor, and EGCG were treated to the anoikis status cells, in order to evaluate the factors of anoikis. The result revealed that growth factors or loss of adhesion can increase phosphorylate Akt. In addition, lack of cell adhesion fails to activate pro-apoptotic factors directly. Activity of Erk kinase depends on not only EGFR signaling but also cell adhesion. Akt activation is mainly affected by EGCG whereas Raf-1 activation is controlled by the presence of cell contact. In addition, EGCG increased the level of NFkB, whereas phophroylated PTEN and total PTEN were not different. In this report,increase of NFkB was correlated with Akt phosphorylation, suggesting that EGCG can protect cells from detachment–induced cell death through Akt activation and subsequent NFkB