복숭아순나방붙이(Grapholita dimorpha Komai)는 1985년 국내에 그 분포가 처음 알려졌으며, 2010년 사과원에서 발생이 확인되었고, 과거 몇 년 간 복숭아순나방(Grapholita molesta (Busck))과 혼동되었을 가능성이 높은 것으로 추측되고 있다. 배 과원에서 복숭아순나방붙이의 발생양상을 알아보기 위해 2012년 5월부터 11월까지 경기 안성, 충남 천안, 경북 상주 지역의 조사 포장에서 페로몬트랩을 이용해 조사했다. 복숭아순나방붙이 발생 모니터링 결과, 경기 안성은 6월과 8월~9월에 2회, 충남 천안은 5월, 8월, 9월에 걸쳐 3회, 경북 상주는 5월, 7월, 8월~9월 3회에 걸쳐 발생 Peak를 보여, 지역에 따라 발생 횟수와 시기가 각각 다르게 나타났다. 복숭아순나방붙이와 복숭아순나방은 형태적으로는 물론, 성페로몬 조성성분도 유사해 각 종의 페로몬 트랩에 2종이 혼재되어 유살되는 경우가 많았다. 복숭아순나방붙이 트랩에 유살된 복숭아순나방붙이와 복숭아순나방의 비율이 경기 안성 지역에서는 21.3:78.7, 충남 천안 지역은 51.4:48.6, 경북 상주 지역은 43:57로 나타났다.

The study was conducted to develop the full seasonal phenology model of G. molesta in pear orchard. G. molesta is a multi-voltine insect with four to five generations per year depending on temperature, food resources and geographic location. For precise model construction, information on temperature-dependent development and the distribution of developmental completion of overwinteirng and summer G. molesta population was used. The performance of model was based on single cohort population of G. molesta. The validation of model system was performed with the male moth catches in sex pheromone-baited traps of pear orchards in four pear production regions (Anseong, Icheon, Naju and Ulju), three apple production regions (Andong, Chungju and Geochang), and four plum orchards (Uiseong) of Korea in 2010 and 2011, respectively. The observed phenology of seasonal population of G. molesta was explained by our model system. The predicted dates for the cumulative 50% male moth catches per each generation were within seven days variation per each generation. The precise predictive model of G. molesta adult occurrence could help decision making and enhancing control efficacy.

Many insects overwinter in diapause in the temperate region. Winter diapause often terminates far yearly than our thought, eg. Many in December. After diapause termination, insect becomes in quiescence. The lower developmental threshold of overwintering Oriental fruit moth larvae is in the range of 7.5-8.4℃ depending on the geographic location. We have determined the developmental period of overwintering OFM larvae from the time series interval collection. Collections were made from mid Jan to late March at 10d interval. Collected larvae were held in a cold chamber at 6°C for 5 days, and then subjected at 20°C with 75% R.H., 16:8(L:D).hr. The developmental period from overwintering cocooned larvae to adults was longest in mid. January collections, became shorter after 31 Jan. collection, then gradually decreased until mid March. Cocooned larvae developmental period became shorter after 31 Jan. while that of pupae became shorter after 20 Feb. During this study, female ratio was not different to 0.5. Mortality of winter collected larvae to adults was 45% including 30% parasitism. Partial explanation of the insect development under the lower developmental threshold was discussed; limitation of linear model estimation, temperature data quality and physiological metabolism after diapause termination. From January to February, daily average temperature fluctuated but under the lower developmental threshold. However, daily maximum temperature became noticeably higher than the lower developmental threshold after 20 Jan. However, this result indicates that after late Jan, physiological state of OFM is altered.

The oriental fruit moth (Grapholita molesta) and the plum fruit moth (G. dimorpha) are internal feeders of apples. Their sympatric and similar sex pheromone compositions suggest their recent divergence in speciation. This study aims to determine genetic factors in this speciation by comparing transcriptomes associated in sex pheromone biosynthesis in these sibling species. Total RNAs were collected two female abodominal tips and read by a short read deep sequencing technology using an lllumina HiSeq. Almost 3-4 Gb reads were de novo assembled and resulted in 76,361 contigs of G. dimorpha and 104,463 contigs of G. molesta. More than 70% of these contigs were annotated and classified by a typical GO analysis. Transcriptomes related with sex pheromone biosynthesis were selected and grouped into fatty acid synthase, fatty acid oxidation. These analyses identified sex pheromone biosynthesis machineries

Understanding the spatial pattern of G. molesta and the temporal variation of their patterns are important to develop and maintain pest management programs in fruit orchards. The overwintering larvae of G. molesta pupate early in the spring and new adults begin a flight for several reasons such as mating, seeking resources (food or shelter) and oviposition. It was known that G. molesta presented “low movement activity” and male G. molesta flight behavior was closely related to the proximity of its host crops. Unmated males remain near the site of emergence in order to find and copulate with unmated females. The fruit-bearing status of orchards are important factors for G. molesta movement. To elucidate the spatial distribution and temporal variation of G. molesta within and among orchards, pheromone traps targeting male G. molesta were used because the trap represent a reliable and economic tool for monitoring adult G. molesta populations. The study was conducted in two apple orchards (one is isolated from other fruit orchards and another is surrounded by apple orchards), Andong and in seven plum orchards, Uiseong, 2010. Using spatial analysis by distance indices, the spatial pattern of G. molesta in each sampling date was presented. In the study of the spatial pattern within apple orchard, the index of aggregation (Ia) of isolated orchard were presented >1, indicating an aggregated distribution pattern, from monitoring results. The spatial association between successive monitoring using X (the index of spatial association) was negative during spring season and after then the value was changed to positive. In the experiment of the spatial pattern among orchards, the index of aggregation was >1 in most monitoring date and the index of spatial association was negative during early and late growing season. Factors influencing the spatial-temporal dynamics of G. molesta are discussed.

Grapholita molesta, G. dimorpha and C. sasakii as “internal feeders” are important apple pests in Korea. Three species overwinters around and in apple orchards. New young larvae of three species bore into new shoots or fruits and then feed inside apple. When mature larvae escape from fruits they make holes that reduces the commercial value of fruit. Therefore, understanding the phenological distribution of three species is critical to establish the precise management system for reducing three species population. The study was conducted to investigate the adult emergence of G. molesta, G. dimorpha and C. sasakii using pheromone traps and to forecast the cumulative proportion of each population. This study is second part of consecutive experiment. Data collection was carried out on three commercial apple orchards and one experimental orchard of Giran in 2010 and 2011. The experimental process was same in the study of plum. More than 50% of G. molesta male was occurred in spring season (within 500 degree-days), 2010 and 2011. The adult emergence of G. dimorpha and C. sasakii was linear and sigmoidal pattern in each year. The phenology of C. sasakii was explained well by nonlinear functions and the equation 3, 6, 8 and 11 were selected based on AICc and BIC. The selected equations were validated by the data of present year (2011) in each region. The performance of G. molesta and G. dimorpha was analyzed well by bimodal functions. The importance of phenological model is discussed to develop and maintain a more precise system for multiple pest management on apple orchard.

The study was conducted to investigate the phenological distribution of G. molesta, G. dimorpha and C. sasakii and to estimate the emergence timing of three species in plum orchards. It was known that G. molesta and G. dimorpha are a multi-voltine insect and C. sasakii has one to two generations depending on temperature and geographic location. Three species damage to many economically important fruit tree such as plum, pear, peach and apple. The main emergence time of each species is different depending on host plant and environmental conditions, specially temperature. Therefore, if we have the information of population density and low temperature threshold of a species and air mean temperature of a region in previous year we can predict the phenology of a species in present year. This is one part of consecutive research. Data collection was carried out in seven plum-growing commercial orchards of Uiseong in 2010 and 2011. The commercial pheromone monitoring traps (GreenAgroTech) were used to investigate the flight phenology of three speices. The record of temperature was received from meteorological center close to monitoring orchards. The relationships between degree-day accumulated above the low temperature threshold and cumulative proportion of accumulated moth caught of previous year was used to predict the phenology of three species in present year. The results of G. molesta and G. dimorpha estimated by bimodal functions were better than those analyzed by nonlinear functions. The phenology of C. sasakii was analyzed well by nonlinear function and the equation 3, 6, 8 and 11 were selected based on AICc and BIC. The selected equations were validated in each orchard.

The study was conducted to investigate the spring emergence pattern of G. molesta and to forecast the emerging time of overwintering G. molesta on tree fruit orchards. G. molesta is one of major insect pests on fruit trees in Korea. The host range of G. molesta includes many economically important tree fruit plants such as apple, pear, peach and plum. The overwintering G. molesta emerge from late March as an adult lay eggs on the shoot of peach or fruits of apple, plum and peach. Therefore, it is important to understand the biofix and to forecast the emerging peak period of overwintering G. molesta for establishing the pest management strategy. The pheromone trap of G. molesta has been utilized to monitor the population density in apple orchard. The commercial stick trap (GreenAgroTech) and lure (Z8-12:AC, E8-12:Ac, Z8-12:OH, 95:5:1) was set to monitor the population density of G. molesta on each place (56 different fruit orchards). The record of temperature was received from meteorological center close to monitoring orchards. The parameters for forecasting the emerging time and peak period of overwintering G. molesta were calculated from the results of Yang et al (1997 and 2001). Although the estimated biofix of G. molesta was not fitted well, the peak period of overwintering G. molesta was explained by linear regression model. The spring emergence pattern of G. molesta was presented differently related to host plant and geographical location. The peak period of G. molesta at the same mornitoring county was presented differently according to host plant. The synchronization between host plant and G. molesta may be studied to figure out the spring emerging time of overwintering G. molesta.

A remote sensing pheromone trap called IT pheromone trap (Korean patent: 10-0982563) was applied to monitor overwintering population changes of the oriental fruit moth, Grapholita molesta, for three successive years in apple orchards. Males of the overwintering populations were attracted during April and May. However, the occurrence peak was delayed and extended to early June in 2010, at which the average spring temperature was significantly lower than the previous years. These overwintering populations could be monitored by the remote-sensing pheromone trap. When the remote-sensing pheromone traps were deployed to apple orchards of different provinces in Korea in 2010, the maximal overwintering populations of G. molesta were monitored at May in all areas. However, the population sizes monitored were significantly different among different localities. This study suggests a practical application of IT pheromone trap to monitor G. molesta in field conditions.

This study investigated the efficiency of mating disrupters for the control of Grapholita molesta(Busck) and Adoxophyes orana in pear orchards. G. molesta was shown 4 peak occurrence, which were different according to treatments. In conventional control, many moths were captured in mid and late April and peaks occurred in mid June, August, and early-mid September. In the non-control treatment, there were low populations in April. A. orana also appeared to 4 peak in 2007. Moths were rarely captured in mating disrupter treatment but many were captured in both the non-control and in conventional treatment. A. orana damage differed significantly between mating disrupter use and conventional control in 2006. Especially, the difference was significant between conventional control and mating disrupter to leaf damage in August, and there were similar results in the damage by G. molesta in the shoots and fruits.

국내 사과 및 복숭아의 과실류에 피해를 주고 있는 복숭아순나방(Grapholita molesta)은 과실 내부 가해라는 서식 습성에 따라 화학적 방제에 어려움이 있 다. 따라서 야외 노출 발육 시기인 성충을 대상으로 교미교란 처리 기술이 대체 기술로서 제안되었다. 본 해충의 성페로몬을 기반으로 교미교란제를 처리한 포장에서 뚜렷한 교미교란 효과를 얻을 수 있다. 그러나 국내 과수의 소규모 경 영규모에 따라 인접한 농가에서 교미한 암컷의 유입으로 방제의 실효를 거두 지 못하게 되었다. 이를 해결하기 위해 교미한 암컷을 포획할 수 있는 먹이트랩 과 처리 효과의 사각지대를 줄이기 위한 교미교란제의 테두리 처리가 병행될 필요가 있었다. 먹이트랩과 테두리처리가 투입된 교미교란제 처리는 교미교란 제의 단독 처리에 비해 우수한 방제 효과를 나타냈다. Paste 형태의 교미교란제 를 3월과 7월의 2회 처리로 연중 방제 효과를 거둘 수 있었다. 추후 교미교란제 의 투입량을 줄이는 기술과 교미한 암컷의 포획 효율을 높이는 유인제 개발 기 술이 필요하다.

Mating disruption (MD) using synthetic sex pheromone lures has been used to control the Oriental fruit moth, Grapholita molesta (Busck), in apple orchards. In Korea, where several small apple orchards are clustered but independently managed, its efficacy has been suspected mainly due to immigration of any mated females from nearby untreated cultivating areas. This study developed an edge treatment technique to decrease any local MD-free zones in a specific MD-treated farm and to trap any immigrating mated females by installing MD lures and food traps around the apple farm with 10 meter intervals. The addition of the edge treatment to the MD significantly prevented leaf and fruit damages induced by G. molesta compared to MD only. Moreover, this study tried to optimize the MD control technique by determining frequency of MD application. It suggests two MD applications with the edge treatment at the end of March and at the early of July to be effective throughout the entire apple growing seasons.

Monitoring was conducted to investigate the occurrence of Grapholita dimorpha Komai in Korean apple orchards using sex pheromone traps. G. dimorpa showed four peaks per year: early May, from late June to early July, from late July to mid August, and from late August to September. After adult emergence of the over-wintered G. dimorpha, G. dimorpha catches was decreased significantly and increased again after July. In G. molesta traps, G. molesta and G. dimorpha were trapped by 98.8 and 1.2%, respectively. Conversely in G. dimorpha traps, G. dimorpha and G. molesta were trapped by 99.7 and 0.3%, respectively. The 30.6% of the moths from the damaged apple fruits were G. dimorpha. This is the first report on G. dimorpha in apple orchards in Korea.

Major fruit moths in commercial apple orchards are known as Carposina sasakii and Grapholita molesta. In addition, recently G. molesta is dominant species compared with C. sasakii in apple orcahrds. In case of Japan, the fruit damage occurred a lot unexpectedly in apple orchards of the northern Nagano during the harvesting season in 2004. As the result of identifying after emerging the larvae collected from the damaged fruit, Grapholita dimorpha was found out. The morphology of Grapholita dimorpha are almost similar to that of Grapholita molesta, and the shape of the damaged fruits was too similar to distinguish them. We installed sex pheromone traps of Grapholita dimorpha in apple orchards to check if there were Grapholita dimorpha in Korean apple orchards and to survey seasonal occurrence. As a result of survey by installing sex pheromone traps of G. dimorpha at one apple orchard in Uiseong-Gun and Gunwi-Gun area respectively, we could find high population density of G. dimorpha in sex pheromone traps. The peak of the first generation of G. dimorpha was dated in early May. The first generation occurrence of G. molesta in sex pheromone traps was lower than that of Grapholita molesta. However, it had higher occurrence of sex pheromone traps than G. molesta since it increased after mid and late July. Also, as a result of identifying in G. molesta traps, there were 673 G. molesta and 8 G. dimorpha. As a result of survey of 1,102 G. dimorpha in its sex pheromone traps, there were 1,099 G. dimorphaand 3 G. molesta.

Local and seasonal populations of the oriental fruit moth, Grapholita molesta , were monitored with sex pheromone trapping and RAPD (random amplified polymorphic DNA) molecular marker to analyze their movement in apple orchards. To detect their movements among farms, pheromone traps were placed at regions between apple farms (‘outside-farms’) as well as within-farms (‘inside-farms’). Four seasonal adult peaks were evident in apple-cultivating fields from April to October in both trappings of inside- or outside-farms. After overwintering generation, populations of inside-farms were significantly reduced with frequent insecticide applications, compared to populations of outside-farms. Within apple farms, G. molesta tended to be unevenly distributed because of significant sublocal preference. Active movements of local and seasonal populations of G. molesta were supported by gene flow analysis using RAPD marker. Monitoring data using sex pheromone and seasonal reduction in initial genetic differentiation detected in the overwintering populations suggest that there must be significant movement of G. molesta among different orchards in apple-cultivating areas.

Mitochondrial genome is inherited in maternal origin without recombination by mating and its specific regions have been used to monitor insect pest populations in agriculture. The oriental fruit moth, Grapholita molesta, is a serious pest on apple industry by its direct damage on fruits. This study reports a full sequence of mitochondrial genome of G. molesta. Sequence contigs were made by primary PCRs on conserved regions and subsequent PCRs to fill the gaps. Annotated genes were highly matched to the sequences of other lepidopteran species. However, a few positions of tRNA genes on the genome were different to other mitochondrial genomes.

Local and seasonal populations of the oriental fruit moth, Grapholita molesta, were monitored with sex pheromone trapping and RAPD (random amplified polymorphic DNA) molecular marker to analyze their movement in apple orchards. To detect their movements among farms, pheromone traps were placed at regions between apple farms ('outside-farms') as well as within-farms ('inside-farms'). Four seasonal adult peaks were evident in apple-cultivating fields from April to October in both trappings of inside- or outside-farms. After overwintering generation, populations of inside-farms were significantly reduced with frequent insecticide applications, compared to populations of outside-farms. Within apple farms, G. molesta tended to be unevenly distributed because of significant sublocal preference. Active movements of local and seasonal populations of G. molesta were supported by gene flow analysis using RAPD marker. Monitoring data using sex pheromone and seasonal reduction in initial genetic differentiation detected in the overwintering populations suggest that there must be significant movement of G. molesta among different orchards in apple-cultivating areas.

An unidentified moth was captured in sex pheromone traps of the oriental fruit moth, Grapholita molesta, especially at spring season in apple orchards and their vicinity. Though the captured males were similar in appearance to G. molesta males, they were easily distinguished by a marked difference in body size. Their occurrence pattern was also similar to that of overwintering G. molesta population from April to May, at which more males were captured in the pheromone traps installed in the vicinity of apple orchards than within apple orchards. After May, they were no longer captured in the pheromone traps. To investigate any larval damage due to this unidentified moth, molecular markers needed to be developed. Four PCR-RFLP markers originated from cytochrome b region of mitochondrial DNA could distinguish this unidentified moth from G. molesta.

A wax-typed pheromone dispenser has been developed and applied to control outbreak of the oriental fruit moth, Grapholita molesta, in apple orchard. To optimize its application technique, this study analyzed effect of different amounts of the pheromone dispenser on mating disruption (‘MD’) of G. molesta. Different pheromone dispenser amounts significantly influenced the MD effect assessed by cumulative male adult catches monitored respectively by sticky delta trap and food trap, and resulted in differential damage on host plants. In a field test during entire growing season, a standard amount (120 g per 0.117 ㏊) of wax-typed pheromone dispenser was proved to be effective to suppress outbreak of G. molesta adults and to prevent host plant damage as much as a current commercial MD product (Isomate<SUP>Ⓡ</SUP>). This study also demonstrated an effectiveness of deployment of food trap barriers around MD-treated area to prevent immigration of mated females from outside untreated areas. These results indicate that the wax-typed pheromone dispenser can be applied to control field G. molesta populations and its co-application with food trap barriers would be optimal to maximize MD efficacy.

Spring phenology of the oriental fruit moth, Grapholita molesta, was monitored using sex pheromone traps in apple cultivating areas. Their occurrence was earlier in southern areas and their population sizes were significantly different among orchards even in a local cultivating zone. The overwintering populations appeared to move between local orchards, based on the fact that monitoring data obtained at the sites between orchards were similar to those of nearby orchards. However, within orchards, these adult movements appeared to decrease and showed skewed occurrences at the side of upwind direction or close to neighboring orchards. At initial occurrence peak (April 20-25), the overwintering populations of the different localities were collected and analyzed in their genetic distances. PCR-RAPD analysis indicated that there were significant genetic differences among the overwintering populations of G. molesta. This genetic differentiation of overwinterin populations may be due to genetic bottleneck following differential selection pressures against the subpopulations of G. molesta during winter on the basis of the RAPD analysis that each early spring population was significantly different to its previous fall population in the same locality.