Background : Stable ginseng production is highly dependent on specific climate conditions. Recently, ginseng yield and quality are negatively affected by climate changes, particularly global warming. Thus, it is imperative to apply a new systematic cultivation method and to develop new varieties with enhanced heat resistance to cope with elevated temperatures. Up to date, ginseng breeding program has mainly focused on the quantity and process ability of red ginseng. New varieties with increased resistance to diverse abiotic stresses need to be developed. Methods and Results : In this study, 13 varieties and 100 germplasms were screened for resistance to heat stress. To measure heat resistance, seedlings were transplanted to a pot 3 times in threes. Two months later, the pot was placed in a temperature chamber at 46℃ for 1 hour, a critical condition that ginseng appears to stop cellular respiration. After waiting for 1 day, the pot was examined for survival rate and fluorescent reaction. Fluorescent reaction was tested according to Nedbal et al. (2000) and Park et al. (2010) and with an image fluorometer. After testing chlorophyll fluorescent reaction, Fm/Fo, Fv/Fm and Rfd were used as screening indices for high temperature resistance. It was found that the adequate values for Fm/Fo, Fv/Fm and Rfd were over 2.2, over 0.55 and over 0.8, respectively. Also, the degree of the above-ground damage is investigated in the 4-year old field. Under our heat stress conditions, susceptible varieties (Chunpoong) and germplasm lines showed drooping and wilting leaf phenotypes, whereas the leaves of a resistant line (named Eumseong 11) remained healthy. Eumseong 11 had the highest values with 2.3, 0.61 and 0.98 in fluorescent test. In addition, compared to the susceptible lines which were wilted in 4-year old field, no detrimental phenotypes were observed in Eumseong 11. Conclusion : ‘Eumseong11’ was selected as a superior line with increased resistance to heat stress. We are now testing how Eumseong11 responds to other abiotic stresses. Our effort will contribute to increase the farmers' income.

Background : Recently, some of the previous stuies reported that was useful technique on growth and yield of organically grown ginseng transplantation in a rain shelter greenhouse. This study was conducted to investigate the optimum method of greenhouse shading for ginseng(Panax ginseng C. A. Meyer) cultivaton in the northern area of Ganwon, Korea. Methods and Results : We carried out to select optimal shade materials and light-penetrated ratio among polyethylene film with two-layered polyethylene net(PEF+PEN) and blue-white duplicated PE film(BWD-PEF) in the condition of greenhouse for ginseng cultivation. The order of light-penentrated ratio by shade meterials was PEN(75%)+PEF 〉 PEN(85%)+PEF 〉BWD-PEF(85%) 〉BWD-PEF(90%) and the order of air temperature was BWD-PEF(85%) 〉BWD-PEF(90%) 〉PEN+PEF(85%) 〉PEN+PEF(75%). The net photosynthetic rate was higher in PEN(75%)+PEF than other shading material treatments during growth season including summer high-temperature period. The root weight and yield were increased by 31.2~55.0% and 25.6~52.2%, respectively under PEN+PEF(75%) compared to other shading materials. Conclusion : We concluded that the PEN+PEF(75%) could be a good shading meterails of the greenhouse for organic 4-year-old ginseng cultivation in northern area of Gangwon, Korea.

Background : The natural stable isotope ratio of common bio-elements like carbon (C), nitrogen (N), oxygen (O), or sulfur (S) varies with diverse isotope fractionation processes in nature. Therefore, measuring the variation of these stable isotope ratios in ginseng roots can be a feasible tool to discriminate the geographical origins of ginseng in Korea. Methods and Results : The 3-year-old six Korean ginseng cultivars were cultivated at the five regions in Korea, and then used for measuring the stable isotope ratios of C, N, O, and S by isotope ratio mass spectrometry (IRMS). The mean C, N, O, and S stable isotope ratio values in the ginseng roots significantly differed according to the cultivation regions (p < 0.05). However, these isotope ratios in ginseng roots had relatively weak discriminative power against to the ginseng cultivars at each cultivation region. The interaction of the cultivation region and ginseng cultivar type also significantly affected to the C, N, O, and S stable isotope ratio in ginseng roots (p < 0.0001). The two-dimensional plots associated with the N stable isotope ratio can effectively separate the ginseng roots in Jinan compared to those in the other regions. The partial least squares-discriminant analysis showed more significant separation between ginseng geographical origins compared to the principal component analysis. Conclusion : Our findings improve our understanding of how the isotope composition of ginseng roots varies with respect to cultivation regions and cultivars, and suggest that the analysis of the stable isotope ratios combined with chemometrics can be used as a feasible tool to discriminate geographical origin of ginseng in Korea.

Background : This experiment was carried out to improve the labor-intensive task to construct of the traditional ginseng shading system in Korea. In this experiment the selected shading materials was new 6 kinds that HDPE - knitted- monofilament and taped PE Net, with taking into account of the amount of light and the amount of leakage to screen the UV stabilized 0.3% - 0.6%. Compared with the traditional shading material, the black-shading material (wt. 200g/㎡) and the yellow shading material (wt. 300g/㎡) was suitable for the ginseng growing environments in Korea. Methods and Results : The installation of shading system was the 45 degree slope -2 meter height from the ground with the selected shading materials for the wide structure ginseng cultivation. Temperature and light intensity was measured with a week interval from the surface to a height of 30cm. The two kinds of the 1st selected materials and the traditional black + blue two layer shading materials, aluminum screen, Canada Gintec shading material, total 5 kinds of materials were installed in wide structure. The growing environmental analysis was measured using data logs (watchdog 1600series). Conclusion : In the first selection of shading materials for Ginseng wide structure, the levels of light intensity were higher in order of blue-shading> Aluminum- shading> black-shading order. The temperatures of the under wide structures were lower in order of the black-shading< aluminum-shading screen< blue-shading order, especially the temperature of the black shading material was lower than the outside temperature. For the second selection of shading material of the ginseng wide structure, the black and yellow shading material were superior to the other materials, in the amount of light shading material and growing conditions and the degree of leakage etc. The temperatures of mid-May in wide structures of ginseng cultivation were higher in order of aluminum-screen> Canada shading materials (Gintec Co.) 2 layer material> Yellow shading> black shading order, and also the amounts of light intensity also was shown to be with the same manner

Background: Developing new ginseng cultivars is a significant time-consuming process owing to the three years of growth required for ginseng to flower. To shorten the ginseng breeding process, it is necessary to establish rapid progression through each generation. In this study, we examined it was possible to rapidly break ginseng seed dormancy using gibberellic acid (GA3) treatment and alternating temperature. Methods and Results: Seeds were obtained from local variety. Seeds were treated with either GA3 at a concentration of 100 ㎎/ℓ, constant temperature (−2℃ and 2℃), alternating temperature (2℃ followed by −2℃, followed by 2℃) or a combination GA3 and temperature treatment. Following experimental treatment, seeds were sown into trays and placed in a greenhouse. Low germination rates were observed in seeds that did not receive GA3 treatment, which were similar following 2℃ and −2℃ constant temperature treatment. Germination rates increased in proportion to GA3 and more so when combined with alternating temperature treatment. In additon, stem and leaf lengths of the resulting ginseng plants were increased following GA3 treatment, although no synergistic effect was observed with alternating temperature treatment. Conclusions: These results suggest that a combination GA3 and alternating temperature treatment enhances ginseng seed germination, which can contribute to shortening the time required to progress through a single ginseng generation for breeding.

Background: The photosynthetic efficiency cool-season, semi-shade ginseng is normal at low morning temperatures, but drops at high afternoon temperatures. Therefore, optimal plant performance would be ensured if it were possible to control daily light transmission rates (LTR). Methods and Results: Plants were grown in a controlled light environment that replicated 11 AM conditions and comparatively analyzed against plant grown under normal conditions. Growth in the controlled light environment resulted in a 2.81 fold increase in photosynthetic efficiency with no change in chlorophyll content, although LTR were high due to low morning temperatures. Increased aerial plant growth was observed in the ginseng plants adapted to the controlled light environment, which in turn influenced root weight. An 81% increase in fresh root weight (33.3 g per plant on average) was observed in 4-year-old ginseng plants grown in controlled light environment compared to the plants grown following conventional practices (18.4 g per plant on average). With regard to the inorganic composition of leaves of 4-year-old ginseng plants grown in controlled light environment, an increased in Fe content was observed, while Mn and Zn content decreased, and total ginsenoside content of roots increased 2.37 fold. Conclusions: Growth of ginseng under a favorable light environment, such as the condition which exist naturally at 11 AM and are suitable for the plant's photosynthetic activity creates the possibility of large scale production, excellent-quality ginseng.

Background: This study examined the use of new bio-materials with enhanced value and functionality, which were derived from fermented wild ginseng cultures. Methods and Results: To examine the antioxidant activity associated with biological functions, radical scavenging analyses (2,2- diphenyl-1-picrylhydrazyl, DPPH and 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid, ABTS) and superoxide dismutase (SOD)-like activity analyses were conducted. Furthermore, the total phenolic and flavonoid contents of wild ginseng fermented with microorganisms (Leuconostoc mesenteroides, Bacillus circulans, Bacillus licheniformis and B. subtilis subsp. inaquosorum) were evaluated to determine the antioxidant activity increment. Regarding ginseng fermented with B. licheniformis, values of 70.6 ± 1.4%, 44.3 ± 1.7%, and 88.4 ± 1.3% were measured using DPPH, ABTS, and SOD-like antioxdiant activity analyses, respectively. The total phenolic content in ginseng fermented with B. licheniformis was 184.5 ± 0.9 ㎍• GAE/㎖, and the total flavonoid contents was 108.5 ± 1.8 ㎍• QE/㎖ in ginseng fermented with L. mesenteroides. Conclusions: Of the four types of lactic acid bacteria examined, the use of B. licheniformis to ferment ginseng resulted in greatest increase in antioxidant activity. Therefore, ginseng fermented by microorganisms might be used to produce functional bio-materials.

Background : New ginseng variety “Geumwon” is appropriate for the cultivation in Chungnam. As an effort to develop new ginseng variety for regional specialization, it was collected from the Geumsan ginseng fields, selected and fixed and then was registered as new ginseng variety in 2015 through the verification of production capacity, farm demonstration and regional adaptation test. It is excellent in underground growth, disease-resistance and yield-ability compared to candidate varieties. Methods and Results : Regional adaptation tests were conducted for new ginseng variety “Geumwon” in 3 regions(i.e. Geumsan, Gochang and Icheon). The results suggested that the leaf length and width of 2-year old ginseng was 7.9cm and 4.2cm in 3 regions on an average basis. Anthrax incidence rate was about 2.5%, which was smaller than that of candidate variety(3.6%), and the underground root weight was 5.6g, which was 34% higher than that of candidate varieties. The leaf length and width of 3-year old ginseng was 9.4cm and 4.5cm, and anthrax incidence rate and leaf soot incidence rate was 0.8% and 1.2% respectively, which was lower than that of candidate varieties. The the root weight, root diameter and hull length of “Geomwon” were superior to that of candidate varieties by about 24%, 6% and 9% respectively. In addition, red discoloration and root rot incidence rate were also lower than that of candidate varieties. The growth of 4-year old ginseng was also superior to that of candidate varieties in the order of Geumsan, Ichoen and Gochang, and the anthrax and leaf soot incidence rate was also lower than that of candidate varieties(about 2.2.% and 2.3 %, respectively). Conclusion : The results of this study suggested new ginseng variety “Geumwon” is easy to cultivate in Chungnam because of its superior underground growth and excellent quality, and it was also evaluated to be an excellent variety that would contribute to the specialization of the ginseng cultivated in Geumsan.

Background : The authors of this study analyzed the effect of the green manures cultivated in the soil of the preparation field in order to select the green manures suitable for ginseng cultivation and the effect of the green manures on the variation of ginseng growth development, the content of crude saponin and yield ability with the aim to use the results as the primary data for improving the quality of ginseng. Methods and Results: To analyze the effect of the application of green manures on the growth of ginseng, the authors cultivated 4 varieties of green manures and installed blue polyethylene sheet and one-layered black polyethylene net+three-layered blue polyethylene net(OBPNTB) and sun-shading material on the managed soil by re-applying green manures and then cultivated ginseng. The results suggested that the growth of ginseng was excellent in the barley+hairy, barely and rye cultivation plot in the plot treated with OBPNTB rather than the cultivation plot teated with Blue polyethylene sheet(BPS) and two-layered blue & two-layered black polyethylene net(TBTBPN), and the growth of leaf length and stalk diameter was excellent in barely + hairy vetch material. For the underground root weight, the growth was the most excellent(30.19g) in the barley+ hairy vetch cultivation plot treated with OBPNTB. For the underground red discoloration and root rot, the incidence rate was the lowest in the plot treated with BPS in which the water leak in a levee was small than in the test plot treated with sun-shading net. For the ginsenoside content in the cultivation plot treated with TBTBPN, the ginsenoid content was the highest in the rey cultivation plot, and it was the highest in the cultivation plot treated with OBPNTB. Conclusion : The results of study suggested rye among green manures is the most suitable for the growth of ginseng; underground growth was the most prominent in the plot treated with sun-shading net material (black 1 + blue 3) and the underground ginsenoside content was the most prominent in the rye cultivation plot and the plot treated with the sun-shading sheet.

Background : Green house hydroponic ginseng in the production cycle is shorter than the open field cultivation growers and attention. In particular, this part of the Aerial is a study on the active ingredients and contents. Ginseng has been focused on the past producing soil cultivation, producing hydroponic ginseng aerial part is known to have a high content of ginsenosides, and the active ingredient. Irrigation method, the culture soil and nutrient management are the impacts associated with the product's performance on ginseng growth. Ginseng growth stage is divided into five stages: emergence, foliation, root elongation, root enlargement and defoliation. because ginseng requires a water adjustment for each growth stage. It has been trying to control bottom surface irrigation and nutrient concentrations. Methods and Results : Ginseng seedling has been used for experiments to screen a healthy seedling of around 0.8g. Each of the seedling transplanted box was water supplied to the timer and the individual nozzle was 4ℓ amount per hour. All growth measures and sampling was carried out four times a seedling transplantation from 30 to 120 days. Soil sampling each time was a chemical analysis. In addition, the plant was used to analyze the ginsenosides. Conclusion : 12 of total ginsenosides ingredients were highest in the aerial part is 90 days, total ginsenosides of the 10 components in the root part was the highest in 30 days. The results were different this ginsenosides content from time to time, there were differences with previous reports. Results are shown to be due to the difference in the cultivation method and environment.

Background : An important feature of the nutrient solution is that they affect not only the growth but also quality of crops by changing nutrient uptake, especially due to changes of EC in nutrient solution. This study was carried out to investigate effect of EC in nutrient solution on growth and ginsenoside of ginseng. Methods and Results : EC in nutrient solution was controlled with 0.68, 0.84, 1.23, 1.41 dS/m. The root weight of ginseng treated by low EC levels in nutrient solution was higher during the initial of growth. However, the higher EC levels, the more increased the change rate of root weight from the initial to the middle of growth. The highest amount of ginsenoside was changed by growth period. Although the total amount of ginsenoside in root is highest treated by EC 0.68 dS/m at 45 days after treatment. the total amount of ginsenoside in root is highest treated by EC 1.23 dS/m at 135 days after treatment. Conclusions : EC in nutrient solution should to be controlled depending on the stage of growth and the part of use, i.e. root and leaves, when ginseng is cultivated through nutri-culture.

Background : Boron (B) is an essential element required for the growth of plant. It has a narrow range of optimal concentration from minimum to maximum thresholds than other micro-elements. The study was carried out to investigate to the influence of B excess concentrations on physiological disorder of leaf, growth and mineral concentration of ginseng to obtain basic information for physiological disorder diagnose. Methods and Results : The ginseng cultivar ‘Gumpoong’ was cultivated by hydroponic system for 2 months. The toxicity symptoms which ginseng leaves were curved downwardly and induced to chlorosis after beginning to dry the edge appeared on leaves more than 30 ppm compared to the control(3 ppm). The growth of ginseng was more decreased with higher B concentration. Mn uptake was also decreased as B concentration increased. It was found that B excess hindered the growth of ginseng and was reversely related to Mn uptake. Conclusions : More than B 30 ppm can negatively affect growth and mineral uptake. Consequently, B excess can occur physiological disorder of ginseng.

Background : The Ginseng has been one of the crops that represents Korea, and the main export items in the 1960s, Now it occupies the first exports of agricultural products. The Eco-friendly cultivation of Ginseng was difficult for various reasons. But the cultivation in Green House can be suppressed disease occurrence, when utilizing an environmentally friendly materials has the advantage that can dramatically increase the income by possible organic production. For other crops, it made up a lot of studies on water management, But the studies on water management of ginseng is somewhat lacking. Therefore, this study was conducted to establish appropriate soil- moisture-management-systems within the house of ginseng cultivation. Methods and Results : The type of house is a double steel structure. The first layer was PE film, the second layer was the blue shading net and 75% black shading net. The Soil texture was silt loam and the Soil Series was YEONGOK Series. Install the house on the adjacent paddy soil and upland soil, and planting on April 30, 2011 which was cultivated by the straw mulching. The irrigation start point was the 40, 30, 20kpa, and the end point was treated with 15, 10kpa. Soil moisture measurements were determined by tension meter(Jet Fill) depth 20cm in soil. Growth and Yield Survey was conducted in accordance with the Rural Development Administration (RDA) standard research. Statistical analysis of the results were analyzed using the Microsoft's Excel 2003. Conclusion : In a survey due to differences in soil traits and Irrigation point, from 2 years to 4years of root weight and yield of Ginseng were heavier in the upland, especially 30kpa irrigation point treatment was heaviest than the other treatments. From 5year to 6 Years of ginseng has showed heavier in paddy soil, the most treatment was 30kpa- 15kpa irrigation point. This trend was the same in upland soil. Soil moisture management in the case of silt loam, the humid-state-control was more advantageous than dry-state control in upland and paddy soil.

Background : The research is designed to investigate the optimal cultivation technology and the growth of above-ground and below-ground sections as well as the photosynthetic characteristics for new ginseng variety “K-1” by differentiating the planting density under the conditions of transplanting and direct seedling. Methods and Results : The K-1 variety and hybrid variety (Jakyungjong) were selected for the research and the ginseng varieties were transplanted and directly sown in Yeoncheon area in 2013. The transplanting was made in the form of 5 lines × 9 rows (45 plants), 6 lines x 9 rows (54 plants), 7 lines × 9 rows (63 plants) and 8 lines × 9 rows (72 plants) in each lot (1.65㎡) while the direct seedling for testing was conducted three times in randomly blocked design in the form of 11 lines × 14 rows (154 plants), 12 lines × 14 rows (168 plants), 13 lines × 14 rows (182 plants). Various measures were collected from the 4-year transplanted ginseng and 3-year direct seedling ginseng in 2015 to find out the growth features and photosynthesis of above-ground section (rate of germination, leaf length, leaf width, stem length and leaf area index (LAI)) and the below-ground section (length, diameter, weight and class of roots). Conclusion : After the planting of the ginseng, the germination rate of K-1 for the transplanting was 85.1 ~ 92.0% across different plantation densities while that for the direct seedling was 67.7% ~ 77.9% across plantation densities, thus showing no significant difference between the two planting methods. LAI was higher for the higher planation density for both transplanting and direct seedling. As for the photosynthesis speed, the form of 6 lines × 9 rows showed the higher speed in transplanting while the form of 12 lines x 14 rows showed the higher speed in direct seedling. The photosynthesis of K-1 was higher than that of Jakyungjong. In the 4-year ginseng cultivated under the transplanting, diameter of roots, number of branch roots and weight of raw ginseng were the highest in the plantation density of 5 lines × 9 rows. The distribution of root weight was high with 23.3% and 20.0% for the 51~70g group and the 71g or above group, respectively, for the 4 year transplanted plants in the form of 5 lines × 9 rows. The growth for above-ground and below-ground sections for K-1 was better than that for Jakyungjong. As a result, it was found that the proper plantation density for the 4-year root in the transplanted K-1 was 5 lines × 9 rows considering the growth of the above-ground section, quantity and distribution of root weight.

Background : The ginseng cool-season and semi-shade plant undergoes photosynthesis smoothly at low temperature in the morning but its efficiency falls at high temperature in the afternoon. Therefore, if it is possible to control light transmission rates (LTR) every day, it will be able to draw an ideal result in the ginseng growth by attracting a lot of lights before 11am and attracting few lights after 11am which is the affect point of the photosynthetic efficiency. Methods and Results : While installing the sun shading facility that could control the light environment based on 11am and surveying the ginseng's growth and photosynthetic characteristics. The photosynthetic efficiency increased 2.81 times compared to conventional practices without the change of the chlorophyll content although LTR was high because of the low temperature in the morning. The aboveground part growth of the ginseng which adapted to the light environment increased to influence the root weight, when the ginseng was 4 years old, the fresh root weight was averagely 33.3g per plant which increased by 81% than 18.4g of conventional practices. Conclusion : If the light environment management according with the photosynthetic nature of the ginseng is conducted based on 11am, it will be possible to produce a large capacity and excellent-quality ginseng.

Background : Ginseng is a semi-shade plant which prefers cool temperature and mild lighting, and artificially installed shading facility is required from preventing photo-oxidation occurrence when ginseng is exposed to direct sunlight. High temperature damages are commonly noticed due to recent global warming although more farmers are using aluminum foil and blue light-proof screen instead of 4-layered polyethylene shade net (blue 3 + black 1) to prevent the rise in temperature and influx of rainwater, there are insufficient research conducted. Methods and Results : In mid-March, 2015, we have installed steel A type shading facility after transplanting 2-year-old domestic variety ginseng in 7 lines, 10 rows per 3.3㎡. We have prevented the excessive influx of direct light by fixing 4-layered shade net, aluminum foil and blue light-proof screen as shading materials on facilities and adding 2-layered black polyethylene shade net. During July and August with high temperature, light transmission rates have shown varying according to shading materials used: blue light-proof screen (8.4%) > aluminum foil (5.9%) > 4-layered shade net (3.3%). In case of the highest temperature reached, the order followed blue light-proof screen (42.3℃) > 4-layered shade net (40.8℃) > aluminum foil (38.3℃), and in case of average temperature it followed blue light-proof screen (48.7%) > 4-layered shade net (37.6%) > aluminum foil (33.2%). Although there were no difference about leaf area among the shading materials, ginseng leaf grown in aluminum foil has lower chlorophyll content than the other two treatment plot because of photooxidation by light damage. The 3-yr-old ginseng root weight harvested in end of October increased as follows light-proof screen (14.4g) > 4-layered shade net (11.9g) > aluminum foil (9.8g). Conclusion : Aluminum foil showed greater influx of light but lower temperature than 4-layered shade net due to its materialistic character. However the front side of foil can reflect light and cause photo-oxidation of rear-planted ginseng leaves, so it is a need for improvement. In case of blue light-proof screen, although it had higher inside temperature, the root weight of ginseng was the heaviest because of less influx of light and destruction of chlorophyll.

Background : Most ginseng seedlings are cultivated in conventional shading facility. However, due to recent climate changes and soil-related bacterial issues, the production of healthy ginseng seedling with its rhizome weigh more than 0.8g has greatly dwindled. Hence, growing seedling system that raises ginseng on nursery soil in greenhouse is being more highlighted. Nevertheless, there are no sufficient researches conducted to study the growth stability after transplantation of ginseng seedling grown at nursery (GSGN). Methods and Results : After categorizing GSGN produced in 2014 according to individual weight (0.5-0.6g, 0.6-0.7g, 0.7-0.8g, 0.8-0.9g), those GSGN with 0.8-0.9g conventional ginseng seedling (CGS) have been transplanted on preparation field in 17, March of the following year. It has turned out that there were no significant differences about seedling emergence rate and weights between GSGN and CGS both same seedling weight of 0.8-0.9g in 25, June after 100 days transplantation. The 2-year-old ginseng growth of transplanted GSGN according to weight has shown strongly positive correlation between the weight of ginseng seedling and their growth. Especially the leaf area and weight have shown significant correlations represented as Y=212.15-567.32X+499.5X2(R2=0.9988) and Y=4.9-12.9X+11.17X2(R2=0.9788), respectively. Meanwhile, there was no significant correlation noticed treatment plot in relationship between seedling emergence rate and chlorophyll content. Conclusion : With the same root weight of GSGN and CGS, it can be judged that the growth of the 2-year-old GSGN is stable.

Background : When ginseng seeds were gathered, the seeds were unripe. To grow immature embryo definitely, special treatment called dehiscence must be performed. Even though dehiscence is completed, most ginseng seeds are on enforced dormancy. The breaking seed dormancy is generally achieved using cold treatment. Also it is reported that gibberellin treatment can replace the treatment. It is very time consuming process in order to develop new ginseng cultivar because ginseng flowers after 3 years of growth. To shorten the ginseng breeding period, it is necessary to establish fast generation progress. Therefore, this study examined the possibility of breaking seed dormancy of ginseng using GA3 treatment and alternating temperature. Methods and Results : Seeds were obtained from local variety fruit which is not inbred. Gibberellin of 100 ppm was treated at seeds for 24 hours. Fixed cold condition was treated on both –2℃ and 2℃. Alternating cold condition was treated on 2℃ and then –2℃, finally 2℃. Fixed and alternating temperature was continued for 15, 30, 45, 60, 90 days that 15 days of alternating temperature is first 2℃ for 5days and then -2℃ for 5days, finally 2℃ for 5days. The other treatment periods such as 30, 45, 60, 90 days mean 10, 15, 20, 30 days respectively. Each of 48 seeds were sowed on tray in greenhouse at 3 replication. Experimental plot was completely randomized. Conclusion : Seeds untreated with GA3 were germinated little and there is no difference between 2℃ and –2℃. Alternating temperature until 60days made no difference with fixed temperature but germination rate increased up to 70.8% when seeds were treated for 90days. Germination of seeds treated with GA3 is much higher than untreated seeds especially combined with alternating temperature.

This research carried out to figure out the effect of the green manure crop cultivated at a preparation field and the shading net on the growth, development, and quality of ginseng. Followings are results obtained from the research. Leaf width of ginseng under the shading net of a two-layered blue and two-layered black polythylene net (TBTBPN) was good at rye and hairy vetch cultured group. Leaf length of ginseng under the shading net of a threelayered blue and one-layered black polyethylene net (TBOBPN) was good at barley and hairy vetch cultured group. Meanwhile, leaf width was good at hairy vetch cultured group. Leaf length of ginseng under a blue polyethylene sheet (BPS) was good at a barley and barley + hairy vetch cultured group, but stem length was shorter compare to other shading net cultivations. Root weight of ginseng was good under the shading net of a two-layered blue and two-layered black polyethylene net (TBTBPN) at a rye and hairy vetch cultured group, and was good under the shading net of a three-layered blue and onelayered black polyethylene net (TBOBPN) at a barley + hairy vetch cultured group, but there was no significant difference under blackout screen according to manure crop varieties. Ratio of rusty root was 10.2% at the barley cultured group under the shading net of a two-layered blue and two-layered black polyethylene net (TBTBPN), and was 23.1% at hairy vetch cultured group under shading net of a three-layered blue and one-layered black polyethylene net (TBOBPN). Ratio of rusty root was the lowest at a rye cultured group regardless the shading nets. Content of the ginsenoside was the highest at the rye cultured group under the shading net of two-layered blue and two-layered black polyethylene net (TBTBPN), was the highest at the barley cultured group under the shading net of a three-layered blue and one-layered black polyethylene net (TBOBPN), and was the highest at the rye cultured group under the blackout screen.

Background : Excessively high concentration of sodium ion causednutrient deficiency and significantly decrease growth. This study was carried out to determine the limiting concentration range of sodium ion in the soil of ginseng field. Methods and Results : The growth of the ginseng cultivar Chunpoong reduced with increase in salinity, and the rate of growth reduction was higher in shoots than that of roots. Particularly, ginseng plants cultivated at high level of nitrate nitrogen or sodium may suffer delayed development and stunted growth. Chlorophyll damage occurred on the leaves of ginseng planted in relatively high levels (> 0.2 c㏖+/㎏) of sodium ion, as determined by the fluorescence reaction. The incidence of physiological disorder in ginseng cultivated at 249 sites was correlated with the concentration of sodium ion in the soils. About 74% of ginseng fields in which physiological disorders occurred had concentrations of sodium ion in soil greater than 0.2 c㏖+/㎏. In contrast, the concentration of sodium ions at 51 of 85 sites where no damage occurred was relatively (0.05 c㏖+/㎏- 0.15 c +/㎏). Conclusions : The concentration of sodium ion in soil of ginseng fields can be classified into three levels optimum (≤ 0.15), permissible allowance (0.15 - 0.2) and excessive (> 0.2).