용액중(溶液中) propanil 분해산물(分解産物)의 분리(分離) 및 동정(同定)을 위해서 2000ppm으로 처리(處理)한 후 2주(週) 간격(間隔)으로 12주(週)까지 일정(一定)한 온도(溫度)로 유지(維持)시키면서 주분해산물(主分解産物)을 조사(調査)한 결과(結果)는 다음과 같다. 추출(抽出)한 혼합물(混合物)로부터 benzene을 전개용매(展開溶媒)로 한 TLC에서 분해산물(分解産物)인 DCA와 TCAB를 Rf치(値) 0.65와 0.94에서 분리(分離)할 수 있었다. GC분석(分析)은 column 온도(溫度) 에서 분리(分離)가 가능(可能)하지만 정량적(定量的) 분석(分析)에서 DCA는 , TCAB는 로 달리하여 분해경로(分解經路)를 조사(調査)하였다. IR spectrum에서 와 의 흡수(吸收) band에 의(依)해 DCA의 작용기(作用基)가 결정(決定)되었다. NMR spectrum에서 와 의 peak는 DCA의proton과 일치(一致)하였다. 이와 같이 용액중(溶液中) 혼합추출물(混合抽出物)로부터propanil의 주분해산물(主分解産物)을 TLC로 분리(分離)하여 GC, IR, NMR로 동정(同定)한결과 propanil은 용액(溶液) 내(內)에서 DCA를 거쳐서 TCAB로 분해(分解)되는 것으로 나타났다.

과수원(果樹園)에 발생(發生)하는 주요잡초(主要雜草)의 연차별(年次別) 변화(變化)와 제초제(除草劑)의 연용처리(連用處理)에 의한 잡초우점도(雜草優占度) 및 군락(群落)의 변화(變化)를 조사(調査)하여 얻어진 결과(結果)는 요약(要約)하면 다음과 같다. 과수원(果樹園)에 발생(發生)된 문제(問題)의 잡초(雜草)는 바랭이, 조뱅이, 이기메꽃, 마디풀, 돌피, 쇠비름 등(等)으로 연차별(年次別)에 비슷하였으며 바랭이가 매년 가장 우점(優占)하는 잡초(雜草)로 판명(判明)되었다. 잡초우점도(雜草優占度)를 나타내는 Simpson's index에서 oxyfluorfen 처리구(處理區)는 1년차(年次)에 0.20이던 것이 3년차(年次)에 0.33으로 다소 증가(增加)하였으나 지수자체(指數自體)가 낮아 특정(特定)하게 우점(優占)하는 잡초(雜草)가 없이 고르게 방제효과(防除效果)가 있었고 무처리구(無處理區)에서는 1년차(年次)에 0.56이던 것이 2년차(年次)에 다소 높았다가 3년차(年次)에는 0.32로 낮아지는 경향(傾向)을 보이면서 잡초초종(雜草草種)이 다양(多樣)해졌다. 잡초군락(雜草群落)의 변화(變化)를 나타내는 비유사성계수(非類似性係數)는 oxyfluorfen 처리구(處理區)에서 1~2년차간(年次間)에 41.6이던 것이 1~3년차간(年次間)에는 58.8로 증가(增加)되어 무처리구(無處理區)보다 연차간(年次間)에 잡초초종(雜草草種)의 변화(變化)가 심하였다. 건물중(乾物重)으로 본 oxyfluorfen의 방제효과(防除效果)는 연차간(年次間)에 차이(差異)는 있었으나 3년(年)동안에 무처리구(無處理區)의 건물량(乾物量) 약 이상에 대하여 95% 이상의 높은 방제율(防除率)을 나타내었고 oxyfluorfen 처리(處理)에 의해서 다년생(多年生)과 이년생잡초(二年生雜草)의 비율(比率)이 증가(增加)하여 총(總) 건물량(建物量)의 약 59%를 차지하였으며 무처리구(無處理區)에서는 70% 이상이 바랭이였다.

An emphasized analysis and reviews on the progress of pesticide managements for the past 10 years through the statistics in Korea are summarized in this continued studies in connection with the fundmental aspects and direction of advanced pesticide industry and improved plantprotection policies for 1980's. Remarkable development and changes are observed in the plant species and varieties, plantation practices and production techniques as well as pest infestations and controls in the last decade, but no normal achievement and operations are recognised on the pesticide management and marketing system especially. Realistic plant protection adminstration and pesticide regulations in accordance to the industrial modernization and pest management advancement must be adjusted in accordance with national economic progress and desirable agricultural structure for 1980's. Special considerations are stated on the strengthening of research and inspection program for the quality products and control with the efficacy and safety use of pesticides. More serious attentions are noted on the over production and flooded stocks under struggled market demands and sales competitions with lethal financial difficulties by producers. Through the status analyzed for the last decade, the integrated past management and cooperative basic control pattern under positive self-forecasting system by farmers are also urged for the effective and economic pest control measures. The problems and solutions discussed here ell the advanced pesticide management as well as the cooperation on the self-ordered quality control and market managing systems in 1980's as it is a desired projection for the further improvement. Most of outstanding and necessary statistics and data in the past decade are also summarized here for references in connection with the previous report.

Background : Cytochrome P450 enzymes belong to the superfamily of monooxygenases that are found in all living organisms with diversity in their reaction chemistry. These P450 enzymes are being used extensively by plants in their defense mechanism. Tetracyclic triterpenes reported as major ginsenoside constituents in Panax ginseng, formed by multiple hydroxylation of cytochrome P450. CYP P450 enzymes is effective in metabolising both natural and xenobiotic compounds. In this study, two ginseng CYP genes (PgCYP76C and PgCYP736A) were functionally characterized by overexpression in heterologous plant system. Methods and Results : In Arabidopsis thaliana and Panax ginseng, planta transformation was generated by floral and cotyledon dipping method using Agrobacterium tumefaciens (C58C1) individually. Spatial and temporal patterns of gene expression were analyzed by qRT-PCR against abiotic and biotic stresses. Herbicide (chlorotoluron) tolerance was performed at seedling stage by in vitro assay to check ectopic expressions. PgCYP736A expression were more abundant in leaves compared to roots and stem of 2-years-old ginseng, where as PgCYP76C gene was found to be highly expressive in rhizome, roots and leaves. Differential expressions were observed in response to abiotic stresses of two ginseng CYP genes, both expressed higher in response to NaCl and SA (salysilic acid) in ginseng. Transgenic Arabidopsis plants overexpressing PgCYP76C gene showed small, rounded leaves and reduced height. This phenotype was found to be caused by down regulation of gibberellin biosynthesis-related genes. Another function revealed tolerance towards herbicides that belongs to phenyl urea. Conclusion : This study indicates the functional roles of CYP genes in plant growth and stress responses. Studied genes also can be used as a tool to make plants herbicide tolerant. Further investigation might focus on it’s function over terpene biosynthesis and defence mechansim against (a)biotic stress.

Background: Cyanazine is used as a pre-emergent herbicide once during the growing season to control weeds of many upland crops worldwide. This study aimed to establish a method to determined cyanazine residue levels in major medicinal crops by using high performance liquid chromatography-UV detection/mass spectometry (HPLC-UVD/MS). Methods and Results: Cyanazine residue was extracted with acetone from the raw products of four representative medicinal plants - Scutellaria baicalensis, Paeonia lactiflora, Platycodon grandiflorum and Angelica gigas. The extract was diluted with a large volume of saline water and directly partitioned into dichloromethane to remove polar co-extractives in the aqueous phase. It was then purifined using optimized Florisil column chromatography. HPLC analysis conducted using an octadecylsilyl column allowed the successful separation of cyanazine from co-extractives of the samples, and the amount was sensitively quantified by ultraviolet absorption at 225 ㎚ with no interference. The accuracy and precision of the proposed method were validated by conducting recovery experiments on each medicinal crop sample fortified with cyanazine at two concentration levels per crop in triplicate. Conclusions: The mean recoveries ranged from 91.2% to 105.3% for the four representative medicinal crops. The coefficients of variation were less than 10%, irrespective of the sample types and fortification levels. The limit of quantification of cyanazine was 0.02 ㎎/㎏ as verified by the recovery experiment. A confirmatory method was performed by liquid chromatography/MS using selected-ion monitoring technique to clearly identify the suspected residue.

본 연구에서는 제초제 저항성인 Ab벼와 해충저항성 Bt벼, 이들의 모본인 동진벼의 현미에서 일반성분, 아미노산, 무기물, 지방산, 비타민 및 항영양소를 분석하여 유전자변형 벼(Ab, Bt)의 영양성분 조성 차이를 비교하고자 수행하였다. 그 결과 무기물의 철분이 모본인 동진벼와 유전자 변형 벼(Ab, Bt) 사이에 약 2배의 차이를 나타내었지만 codex 범위 내에 존재하였고, 비타민 류에서는 Bt벼와 동진벼 간의 유의적인 차이가 나타나지 않은 반면 Ab벼의 비타민 B1, B7, E에서 유의적인 차이를 보였다. 또한 트립신 저해제는 시료 모두에서 0.1 TIU/mg 미만으로 극소량 검출되었다. 총 46가지 분석 성분 중 17가지가 함량 및 조성에 유의적인 차이를 보였으나 이러한 차이는 이들의 모본인 동진벼, OECD 표준기술서, 그리고 일반 상업화 품종의 범위 내에 존재해 영양학적 측면에서 실질적으로 차이가 없는 것으로 판단된다.

It is necessary to carry out a risk assessment to determine the consequences of releasing a particular plant species containing specific transgenes before transgenic plants can be grown under filed conditions. Gene flow from transgenic plants to wild closely related species has raised concern recently. Since transgenic crops were released in 1996, the global area of transgenic crops has been increasing rapidly. The transgene introgression from transgenic crops to their wild relatives is unavoidable in some species. Transgene introgression is of concern because the crop–wild plant hybrids might be conferred with a selection advantage to increase their performance, which could result in negative ecological consequences to natural ecosystems. The genus Brassica has 159 species, including a number of wild species that are of great importance to the economy. Most transgenic Brassica gene flow research has focused on the most successful cross between transgenic oilseed rape Brassica napus and its wild relatives Brassica rapa, a widely distributed weed in the farming system in Europe and America, since the hybridization can spontaneously happen and the generations can backcross to B. rapa easily in the wild conditions. In this study, we aimed to characterize transgene introgression, segregation, and expression in backcrossed generations between tramsgenic B. napus and B. rapa. These results will contribute to the environmental risk assessment and assist in biosafety management.

Rice (Oryza sativa) is the most important staple food of over half the world’s population. This study was conducted to evaluate the possible impact of transgenic rice cultivation on the soil microbial community. Microorganisms were isolated from the rhizosphere of GM and non-GM rice cultivation soils. Microbial community was identified based on the culture-dependent and molecular biology methods. The total numbers of bacteria, fungi, and actinomycete in the rhizosphere soils cultivated with GM and non-GM rice were similar to each other, and there was no significant difference between GM and non-GM rice. Dominant bacterial phyla in the rhizosphere soils cultivated with GM and non-GM rice were Actinobacteria, Firmicutes, and Proteobacteria. The microbial communities in GM and non-GM rice cultivated soils were characterized using the denaturing gradient gel electrophoresis (DGGE). The DGGE profiles showed similar patterns, but didn’t show significant difference to each other. DNAs were isolated from soils cultivating GM and non-GM rice and analyzed for persistence of inserted gene in the soil by using PCR. The PCR analysis revealed that there were no amplified protox gene in soil DNA. These data suggest that transgenic rice does not have a significant impact on soil microbial communities, although continued research may be necessary.

Rice (Oryza sativa) is the most important staple food of over half the world’s population. This study was conducted to evaluate the possible impact of transgenic rice cultivation on the soil microbial community. Microorganisms were isolated from the rhizosphere of GM and non-GM rice cultivation soils. Microbial community was identified based on the culture-dependent and molecular biology methods. The total numbers of bacteria, fungi, and actinomycete in the rhizosphere soils cultivated with GM and non-GM rice were similar to each other, and there was no significant difference between GM and non-GM rice. Dominant bacterial phyla in the rhizosphere soils cultivated with GM and non-GM rice were Actinobacteria, Firmicutes, and Proteobacteria. The microbial communities in GM and non-GM rice cultivated soils were characterized using the denaturing gradient gel electrophoresis (DGGE). The DGGE profiles showed similar patterns, but didn’t show significant difference to each other. DNAs were isolated from soils cultivating GM and non-GM rice and analyzed for persistence of inserted gene in the soil by using PCR. The PCR analysis revealed that there were no amplified protox gene in soil DNA. These data suggest that transgenic rice does not have a significant impact on soil microbial communities, although continued research may be necessary.

Vinegar has been considered as a potential organic herbicide, but more knowledge is needed about acetic acid concentration, application volume and use of adjutants on weed control. In this study, we determíned optímum acetic acid concentration and spraying time on several weed species. Vinegar is more effective in broadleaf weeds than grasses. Vinegar is also effective in controlling younger weeds such as 3rd weeks to 4th weeks after emergency. For example, 3rd weeks after emergency weeds were easily controlled at 2-3% of vinegar solution. But 4th weeks after emergency weeds were controlled at 4-5% of vinegar. Therefore vínegar concentration was recommended about 5%, and application time was when the weeds were young - within 31eaf stage.

생육 중인 호밀 리빙멀치 사이 부분경운 기계화 콩 파종기술체계를 가지고 2007년은 호밀의 파종시기를, 2009년은 시험장소를 달리하면서 잡초억제 및 콩의 생육 및 수량에 미치는 영향을 조사하였는데, 중요한 사항을 요약하면 다음과 같다. 1. 4월초~5월초에 파종한 호밀 리빙멀치에 의한 콩 파종 후 40~50일까지의 잡초억제 효과는 70~90%로 현저히 높았는데, 호밀 파종기에 따른 잡초억제의 차이는 없었다. 또 호밀 리빙멀치의 잡초억제 효과는 콩 수확기 까지 지속되었다. 2. 잡초 초종별로는 잡초발생량의 60~80%를 차지하는 피에 대한 리빙멀치의 잡초억제 효과가 뛰어났으며 일찍 발아한 명아주보다 콩 파종 후 발아한 피, 바랭이 및 쇠비름에 대한 억제효과가 높았다. 3. 호밀을 4월 초에 파종한 것이 4월 말~5월 초에 파종한 것보다 콩 파종 시 리빙멀치량이 많았으며, 그에 따라 콩 초기생육이 많이 억제되었고, 수확기 종실수량의 감소에도 영향을 주는 것으로 나타났다. 4. 호밀을 4월 말~5월 초에 파종했을 때 콩의 초기생육은 억제되었지만 호밀이 고사한 후에 콩의 생육이 많이 회복되었고, 종실수량은 관행재배와 차이를 나타내지 않았다. 2008년 지역별 시험에서는 콩 종실수량은 호밀 리빙멀치 유무보다 지역의 포장조건에 따른 영향이 컸다. 5. 봄 잡초발생 경향, 리빙멀치량에 따른 잡초억제, 콩 초기생육 및 종실수량에 미치는 영향을 고려할 때 호밀 리빙멀치 사이 부분경운 기계화 콩 파종체계에서 호밀의 파종시기를 4월말 이전으로 당기는 것은 바람직하지 않았다.

Seashore Paspalum (Paspalum vaginatum Swartz) is a warm season grass and indigenous to tropical and subtropical regions of coastal areas worldwide. The species is used as feed for cattle and horses and has been very successful for golf courses worldwide. One of the most outstanding characteristics of seashore paspalum is its tolerance to saline soils compared to other warm season turfgrasses. The development of new seashore paspalum cultivars with improved traits could be facilitated through the application of biotechnological strategies. The purpose of this study was to product for herbicide resistant seashore paspalum using Arobacterium-mediated transformation and this study is the first report on transformation and herbicideresistant transgenic plants in seashore paspalum. Embryogenic calli were induced from the seeded variety of pseashore paspalum. Embryogenic calli were transformed with Agrobacterium tumefaciens strain EHA105 carrying the binary vector pCAMBIA3301 with two genes encoding gusA and bar. Transformed calli and plants were selected on medium containing 3 mg/l PPT. PCR detected the presence of the gusA and bar gene, indicating both genes are integrated into the genome of seashore paspalum. A chlorophenol red assay was used to confirm that the bar gene was expressed. By application of herbicide BASTA, the herbicide resistance in the transgenic seashore paspalum plants was confirmed.

A gene flow has been tested from transgenic herbicide-resistant Chinese cabbage to major crops in Cruciferae. Hybridizations were made between transgenic Basta-resistant Chinese cabbage and non-transgenic Chinese cabbage, B. napus, B. juncea and Brassicoraphanus(B. campestris × Raphanus sativus, 2n=4x=38) with honey-bee mediated fertilization. Progeny populations were selected by Basta herbicide. The F1 hybrids with pat gene were again self-pollinated or cross-pollinated with nontransgenic Chinese cabbage and rape naturally or artificially. Pod setting ratio were compared as a tentative parameter for potential gene flow. Key agronomic characters were compared among the F1 hybrid populations obtained. In the days to flowering after cold treatment, the F1 hybrids of Brassicoraphanus x B. campestris and B. napus x B. campestris(GM) were the shortest (75 days), and the GM Chinese cabbage and the F1 hybrid of B. juncea x B. campestris(GM) were 87 and 93 days, respectively. In the stem length, the F1 hybrid of Brassicoraphanus x B. campestris was the longest(175 cm), and followed by the F1 hybrid of B. napus x B. campestris(GM), the F1 hybrid of B. juncea x B. campestris(GM) in order. The naturally occurring pod setting ratios were fluctuated seriously among individual and segregation lines. Natural pod setting of the transgenic Chinese cabbage was 10 to 19 %. The F1 hybrid of B. napus x B. campestris(GM) showed the ratio of 4-11%. However, the F1 hybrids of B. juncea x B. campestris and Brassicoraphanus x B. campestris(GM) did not produced seeds. The F1 hybrids with pat gene were hybridized with nontransgenic Chinese cabbage and rape by artificial bud pollination to test potential gene flow. All cross combinations except Brassicoraphanus produced seeds and the pod setting ratios ranged from 35 to 100 percentages. Thus, the potentials of the gene flow from Chinese cabbage with pat gene to the order specise in the same gene interspecies or intergeneric species and from the F1 hybrids with pat gene to nontransgenic Chinese cabbage and rape were detected. Herbicide resistant test to the acquired seeds by natural and artificial pollinations including honey-bee mediated fertilization using the same materials is going on.

Haploid system by anther culture allows the development of homozygous lines when doubled. The response of anther culture to Basta (glufosinate) resistance was investigated on transgenic plants (cv. Anjungbyeo) in order to identify inheritance of bar gene associated with Basta. Most of the regenerated transgenic plants were sterile, and only a few plants produced viable seeds (A1) in the greenhouse. The bar gene was analysis by PCR in basta resistant transgenic plant (TA0). The transgenic seeds (A1) were significantly germinated in Basta solution compared with non-transformed seeds. As a result of anther culture, in regenerated haploid plants, segregation ratio was 1:1 in five of eight cross combinations. In diploid plants, segregation ratio was 1:1 in seven of eight cross combinations. Although there was some differences in the cross combinations, most of the combinations had 1:1 segregation ratio which supports the theory. The difference may be a result of the small sample size or the difference of anther culture response caused by genotypic difference. Hence, when many cross combinations were anther-cultured the results would support the theory.

Herbicide resistance is the most common trait being tested and thus herbicide‐resistant genetically modified plants are now the most widely cultivated worldwide. Here we developed herbicide‐resistant transgenic Agrostis mongolica Roshev. by employing an efficient Agrobacterium‐mediated transformation procedure with 25.2% of transformation efficiency. The identification and employment of regenerable and reproducible type of callus was one of the most critical factors to ensure success in this study. PCR analysis confirmed that the bar transgene was integrated into the genome of transgenic plants. The expression of 35S‐bar gene was confirmed by Northern blot analysis. The transgenic plants showed complete resistance to herbicide, indicating that the bar gene is functional in transgenic plants.