Haemaphysalis longicornis (Acari: Ixodidae) is a medically important arthropod vector of tick-borne diseases, especially the severe fever with thrombocytopenia syndrome (SFTS). To establish a management strategy against tick-borne diseases, it is fundamental to understand the basic biology of vector species. Therefore under laboratory conditions, we evaluated the horizontal movement of adult and nymph stages of H. longicornis to better understand their dispersal behaviors. In the experiments, field populations were subjected to video recording of the horizontal movement of the ticks in a petri-dish arena for 3 hours. Our results indicate that nymphs showed a significantly higher mobility than adults, whereas there was no significant difference in angular velocity. Nymphs moved 3.74 ± 0.26 m at an angular velocity (AV) of 1.60 ± 0.07 deg/s. and adults moved 2.91 ± 0.26 m at an AV of 1.61 ± 0.14 deg/s.

5개 지역 양계장에서 채집한 닭진드기(Dermanyssus gallinae) 성충에 대한 11종 살비제의 감수성을 조사하였다. Bifenthrin과 formic acid + pyridaben은 100배의 희석배수로 처리한 결과, bifenthrin은 5가지 지역에서 모두 100%의 살비율을 보였으나 formic acid + pyridaben 합제 는 연천과 안성을 제외한 나머지 3지역(경주, 칠곡, 금산)에서 20% 이하의 낮은 살비율을 보였다. 카바메이트계 약제인 carbaryl은 경주(45.0%) 를 제외한 4지역에서 100%의 살비효과를 보였다. 유기인계 약제인 dichlorvos는 연천을 제외한 4지역 모두 100%의 살비효과를 보였지만 연천 은 66.7%의 살비율을 보였다. 연천과 안성지역의 양계장이 살비제에 대한 감수성이 높게 나타났으나 다른 3지역(경주, 칠곡, 금산)의 경우에는 약제에 따라 활성의 차이를 보였다. Clothianidin, thiamethoxam, fenitrothion, formic acid + pyridaben은 지역에 따라 살비활성의 효과에 차이를 보여 사용 시 주의를 기울여야 할 것이나, carbaryl과 cartap hydrochloride, dichlorvos, bifenthrin은 실험에 사용된 모든 지역에서 활 성이 높아 모든 지역에서 사용가능할 것으로 생각된다. 양계장별 살비제 활성이 차이를 보이는바 효과적인 닭진드기의 방제를 위해서는 살비제의 활성평가와 더불어 약제의 교호살포와 무분별한 사용을 지양해야할 것으로 보이며 본 연구가 닭진드기 방제를 위한 기초 자료로 활용될 수 있을 것으로 생각된다.

The two-spotted spider (Tetranychus urticae) is an important pest of many agricultural crops. Laboratory bioassayswere conducted to determine the toxicity of Pyflubumide and Cyenopyrafen against three populations of T. urticae collectedfrom apple orchards. The adults and eggs of three populations all showed susceptibility to Pyflubumide and Cyenopyrafen,as the 50% lethal concentration (LC50) values were all lower than each of the recommended concentrations. There wereconsiderable differences in susceptibility of the acaricides between eggs and adults in different populations. Pyflubumideshowed highest toxicity to adults of Yeongju population (LC50 = 1.40 mg/l) and eggs of Andong population (LC50 =0.54 mg/l). Cyenopyrafen showed lowest toxicity to adults of Andong population (LC50 = 20.29 mg/l) while displayedhighest toxicity to their eggs (LC50 = 2.64 mg/l). These results suggested that T. urticae had not developed resistancein these three regions, Pyflubumide and Cyenopyrafen can serve as alternative chemical candidate for integrated mite managementin apple orchards.

Amblyseius eharai is a native predatory mite in Korea and a candidate of biological control agent to control spidermites. To evaluate the predation ability of A. eharai, its functional response was tested at 10, 30, 50, 70 and 130 larvaeof Tetranychus urticae. A. eharai showed the Type 2 functional response. The attack rate of female and male A. eharaiwas 0.109 and 0.019, respectively. The handling time of female and male was 0.164 h and 0.234 h, respectively. Theattack rate was significantly different between male and females at 95% confidence interval. However, handling time wasnot statistically different. In comparison with Neoseiulus californicus, attack rate and handling time of A. eharai werebetter than N. californicus. These result should be helpful for evaluation of A. eharai for a biological agent against T.urticae.

Hallasan is an elliptical volcano on Jeju Island and is the highest mountain in South Korea. A survey was carriedout to investigate soil Gamasina mite composition in the Hallasan National Park during 2016-17. Soil samples were collectedvarious microhabitats from Baekrokdam and Muljangori. We found these eight species; Euparholaspulus primori, Gamasholaspisbrowningi, G. communis, Parholaspulus bregetovae, P. hiasmaticus, P. maturovae, P. orientalis and P. paradichaetes fromsoil. Among these species, Parholaspulus bregetovae, P. hiasmaticus, P. maturovae, P. orientalis and P. paradichaeteswere first records from Baekrokdam and Muljangori, Halla National Park, South Korea.

Effects of temperature on development and ovipistion of Amblyseius eharai, were examined at 11 constant temperatures (18.0, 20.1, 21.6, 24.0, 24.1, 27.4, 28.6, 30.2, 32.0, 33.2 and 35.9 °C), and six constant temperatures (18.0, 21.6, 24.1, 27.4, 30.2 and 33.2 °C) by using Tetranychus urticae as prey. Developmental rates were fitted with a nonlinear Briere 1 model, which estimated optimal temperatures for the development as 30.6 °C for total immature stage. In a linear model, the lower thresholds and thermal requirements (DD) were 13.2 °C (71.94 DD) for total immature stage. Fecundity was well described with the Extreme value function, which indicated optimal temperatures for the oviposition as 24.3 °C. Overall, findings herein provide comprehensive data and explanations on the temperature-dependent development and oviposition of A. eharai, and prove the probability of A. eharai as a good biological agent for T. urticae in early or late crop seasons, and/or at low temperature cultivation farm lands.

The Ixodid ticks has been suspected to be the vector of severe fever with thrombocytopenia syndrome virus (SFTSV) in Korea. In order to confirm transovarial transmission of Ixodid ticks, a total of blood fed 284 ticks were collected from six host animals. They were identified as 3 genera and 4 species, Haemaphysalis longicornis (97.2%), H. flava (0.7%), Ixodes nipponensis (1.8%) and Amblyomma testudinarium (0.4%). Among them, 192 female adults hatched their eggs successfully. Our results showed a high prevalence of SFTSV among collected ticks (3.1%, 6/192 ticks), and we detected SFTSV from their larvae. This result suggests that the parent generation of H. longicornis can transmit SFTSV transovarially into their F1 progeny and H. longicornis might be one of major vectors of SFTSV in Korea.

Amblyseius eharai is a good candidate for biological control agent for major agricultural pest mites such as twospotted spider mites, rust mites, etc. in orchard systems. A. eharai is a native predatory mite of East Asia, and is one of the abundant predatory mites in orchards in Korea. However, ecological studies on this species including its reproductive ability have not been studied yet. Thus, in this study, temperature-dependent developmental rate of adult A. eharai, and three major oviposition related models were studied using six constant temperatures (18.0, 21.6, 24.1, 27.4, 30.2 and 33.2 ℃) with Tetranychus urticae as prey. Temperature-dependent developmental rate, survival rate and oviposition rate of A. eharai adults were well described with a non-linear equation (r2=0.99), a reverse sigmoid function (r2=0.94), and a Weibull function (r2=0.98), respectively. The fecundity was fitted with an Extreme value function (r2=0.99) and the highest in 24.3 ℃. These results would be helpful to verify the abilities of A. eharai as a bio-control agent in the fruit trees.

Ixodid ticks are notorious as the vector of severe fever with thrombocytopenia syndrome virus (SFTSV) in Korea. To confirm the transovarial transmission of SFTSV, we collected blood-fed adult ticks from animals in SFTS case reported area of Jeju island in 2015. A total of 476 ticks were collected from four host animal species, horse (81.3%), dog (9.0%), roe deer (7.1%) and wild boar (2.5%). They were identified as 1 genus and 2 species, Haemaphysalis longicornis (87.8%) and H. flava (12.2%). Among them, 131 adults hatched their eggs successfully. The body weight of females was highly correlated with the number of egg masses laid (P < 0.05). We confirmed that one adult H. flava was infected with SFTSV and its infection rate was 0.77%. The eggs of the SFTSV-infected adults also were infected with SFTSV and its transovarial transmission was confirmed. This result suggests that the parent generation of H. flava could transmit SFTSV transovarially into their F1 progeny and H. flava might be one of major vectors of SFTSV in Korea.

The American house dust mite, Dermatophagoides farinae Hughes, 1961 (Acari: Pyroglyphidae), is recognized as an important source of allergens in the domestic environment. An assessment was made of the toxicity of constituents from essential oil of cade, Juniperus oxycedrus L. (Cupressaceae), and related compounds as well as four experimental spray formulations containing the oil (10–40 g/liter sprays) to adult D. farinae. In a contact + fumigant mortality bioassay, methyleugenol (24-h LC50,5.82 μg/cm2) and guaiacol (8.24 μg/cm2) were the most toxic constituents to the mites and the toxicity of these constituents and benzyl benzoate did not differ significantly.

무가온 시설토마토 재배지내에서 토마토녹응애 발생양상은 4월 중하순경 최초로 발생하기 시작하여 6월 중하순경 최대 발생을 나타냈다. 하지만 야간기온이 낮을 경우 번식력이 증가하게 되고 주간기온이 높을 경우에는 분산이 활발해져 고온기인 7월에도 지속적으로 증가하는 것으 로 조사되었다. 유기농업자재 11종에 대한 토마토녹응애의 살충독성 실내검정 결과, EOIMa (Matrin 0.6%), EOICo (Clove oil 10%)가 500 배에서 각각 92.1%와 95.1%로 90% 이상의 살충효과를 보였다. 특히 EOIMa 1,000배에서는 90.4%로 90%, EOIMa 2,000배에서는 88.2%의 높은 살충효과를 보였다. 포장검정에서도 EOIMa 1,000배와 EOICo 500배는 각각 91.5%와 93.9%의 살충효과를 보여 두 약제 모두 농약을 대 체하기에 충분하였고 EOIMa의 경우 2,000배에서도 살충효과가 90%에 가까웠기 때문에 예방적 활용도 가능할 것으로 판단되었다.

The two spotted mite, Tetranychus urticae Koch, is a global pest, and has developed severe resistance to several types of acaricides. This study compared the development in susceptible (S) and acequinocyl, bifenazate, pyridaben, abamectin and etoxazole-resistant (AcR, BR, PR, AbR and ER) strains of T. urticae by X-ray irradiation. When eggs were irradiated with 100 Gy, the egg hatching was completely inhibited in all strains. When nymphs were irradiated with 200 Gy, their hatching was completely inhibited in all strains. When adults were irradiated, the fecundity and egg hatching was decreased at 150 Gy and above, and hatchability of F1 generation was completely inhibited at 300 Gy in all strains. Adult longevity was slightly increase at 150 Gy and above. In addition, we performed quantitative real-time PCR on several genes. To examine the difference of all strains on radiation stress-induced gene expression, we performed quantitative RT-PCR (qPCR) of several known stress-induced genes.

본 실험은 유기농 신선초(Angelica utilis)포장에서 점박이응애(Tetranychus urticae)의 발생지점 및 확산 양상을 확인하고자 수행하였다. 실험은 17주 동안 강원도 원주시 신림면 용암리에 위치한 유기작목반 비닐온실(625㎡/동)에서 진행되었다. 온실 입구부터 5m 간격으로 확대경을 이용해 해충 밀도를 조사한 결과 A농가에서는 1주차에 입구에서 95m 지점의 해충밀도가 가장 높았으며, 시간이 경과함에 따라 앞쪽으로 확산되었다. 이는 A농가 뒤쪽 우거진 수풀에서 점박이응애가 유입되어 도로변이 있는 앞쪽으로 확산된 것으로 보인다. B농가의 경우 1주차에 입구에서 50m 지점의 해충밀도가 가장 높았으며 시간이 경과함에 따라 뒤쪽으로 확산되었다. 이는 B농가 앞쪽에 있는 신선초온실에서 해충이 유입되어 확산된 것으로 보인다. 또한 점박이응애의 천적을 1주일간격으로 3회 방사한 결과 A농가는 방사 6주차에, B농가는 방사 3주차에 100% 해충방제효과를 보였다. 신선초는 1년 상시 재배되는 작형인 만큼 계절별, 지리적 특성에 따라 해충 양상이 달라질 수 있으므로 추후 체계적인 모니터링을 통해 효과적인 천적방사시기 및 방사지점을 확인한다면 해충발생초기에 소량의 천적투 입으로 방제효과 극대화, 노동력과 방제비용의 절감을 기대할 수 있을 것으로 기대된다.

A survey was carried out to investigate Gamasina mite composition in the Gyeongbuk and Gangwon, during 2009-2014. Samples were collected from wide range of habitats (i.e. forest, riparian grassland, agriculture). 3 species namely Antennoseius avius (Ascidae), Hypoaspis sardous (Laelapidae) and Stylochirus caucasicus (Ologamasidae) are records as new records compared to already existed database of mite in Korea. Description of three new records species, identification keys (female adult) each species and figures of external morphology are given.

The Hawthorn spider mite Amphitetranychus viennensis is an important pest of various fruit trees as well as a variety of ornamental plants. Its presence has been reported in China, Japan, Korea, and middle east and European countries including Grorgia, Russia, Turkey, Ukraine. Thus, it is a quarantine species in American countries and Australia. Intra-specific variation of A. viennensis was investigated using 555 bp of ITS2 ribosomal DNA and 453 bp of the cytochrome oxidase I (COI) gene. Its variation of ITS2 and COI among China, Japan and Korea were 1.7-2.2% and 0.2-0.7%, respectively. In addition, we collected samples from 14 location in Korea. Intra-specific diversity of ITS2 and COI within Korean populations were ranged into 0.5-0.9% and 0.2-0.5%, respectively. Thus, A. viennensis is not much genetically diverse in different populations.

무농약 시설재배 오이에서 잎, 꽃 및 과실을 가해하던 응애를 채집하여 동정한 결과 오이긴털가루응애(Tyrophagus neiswanderi)로 동정되었 다. 오이긴털가루응애는 분류학상 응애목(Acarina) 가루응애과(Astigmata)에 속하며 형태적으로는 유백색의 계란형을 띄고 체장은 490.1㎛, 체 폭은 288.1㎛의 매우 작은 응애이다. 오이긴털가루응애는 잎, 꽃, 과실에 모두 발생하여 피해를 주었다. 피해증상은 정식 초기 유묘의 경우 신초가 수축되면서 연노란색으로 탈색이 되고, 심하면 신초가 멎는 피해가 나타난다. 또한 오이가 생육을 하는 과정에서 잎이 피해를 받으면 처음에는 오이 잎에 흰색의 반점이 나타나고 시간이 경과함에 따라 반점에 구멍이 나고 찢어진다. 꽃에서도 흰색 반점과 찢어지는 증상이 나타난다. 과실에서는 어린 과실일 때 피해를 받으면 과실의 비대가 저해되거나 기형과가 나타나며, 과실이 비대하더라도 코르크증상으로 인해 상품성이 떨어지게 된다.

이 연구는 사과원에서 20년동안 두 종 응애의 밀도변화를 조사하기 위하여 수행하였다. 사과나무를 가해하는 두 종의 응애 해충, 점박이응애 (Tetranychus urticae)와 사과응애(Panonychus ulmi)의 발생을 1992년부터 2011년까지 8개 도시 포함, 한국의 남부지역에 위치한 사과주산지역에 서 조사하였다. 20년간의 추세는 많은 과원이 1992년부터 1999년까지는 점박이응애가 주로 가해를 하였지만 2000년도부터는 사과응애가 우점하 기 시작했다. 사과응애의 평균관찰밀도는 일정했고 점박이응애의 밀도는 이 기간동안 일정하지 않았다. 5년주기별 발생추세 분석은 점박이응애의 밀도가 2002년 이후에 감소하는 것을 보여주었다. 4월부터 9월까지 월별피해과원율과 두종 응애의 평균발생밀도는 사과응애의 밀도가 4월에 높지 만 점박이응애의 밀도가 5월부터 9월까지 높았다. 이런 변화는 살충제 살포횟수, 초경재배관리, 질소비료 사용의 감소와 전체적인 과원관리의 변화 등으로 기인한다. 그러나 이러한 예측은 이를 증명하기 위해 보다 자세한 연구가 필요하다. 본 연구를 통해 비료살표와 같은 재배법과 살충제 살포횟 수와 종합적해충관리 등의 환경적 변화가 사과원에서 두종 응애의 우점종과 발생밀도에 영향을 준 것으로 보인다.

The ectoparasitic honey bee mites was originally confined to the Asian honey bee(Apis cerana etc.), mites had become widely established in the world and have continued to cause extensive bee colony deaths. Mites attach to adults and developing brood, where they feed on their blood. If left untreated, mites can deform bees and eventually kill the colony. Varroa destructor and Tropilaelaps clareae has plagued European honey bees, Apis mellifera. Differences in mite tolerance are reported between two honey bee species A. mellifera and A. cerana. We were counted number of mites(V. destructor and T. clareae) form 20 colonies. V. destructor and T. clareae has very similar behavior in honeybee colony. When behaviorally close, two competing species may populational interfere, and thereby affect their population dynamics. We tested for populational interference (PI) between two populational competing honeybee mites, V. destructor and T. clareae, by investigating their population dynamics when they competed on the same colony.

Phoretic mites were collected from Copris ochus and C. tripartitus (Coleoptera: Scarabaeidae). These beetles were collected from Holoce Ecosystem Conservation Research Institution, Gangwon-do. Five unrecorded species of Eviphis hastatellus Berlese, 1910 (Eviphididae), Holostaspella scatophila Takaku, 1994 (Macrochelidae), Macrocheles japonicus Evans & Hyatt, 1963 (Macrochelidae), Pachylaelaps siculus Berlese, 1921 (Pachylaelapidae) and Parasitus consanguineus Oudemans & Voigts, 1904 (Parasitidae) were recorded from Korea for the first time. However, Parasitus consanguineus Oudemans & Voigts (Parasitidae) was already recorded by Oudemans & Voigts in 1904 but since then no one has recorded. We have rerecorded this species in Korea.

We provide the first report on Stigmaeopsis miscanthi Saito, which was identified from Chinese silver grass Miscanthus sinensis on Ulleung Island in Korea. This species is one of the S. celarius complex, which involves several cryptic species. S. miscanthi has longer second dorsal propodosomal setae (P2), which is an important characteristic for species identification in the genus Stigmaeopsis. We determined nucleotide sequences of the internal transcribed spacer 2 (ITS2) and cytochrome c oxidase subunit I (COI) of S. miscanthi. The COI sequence differed by 0.6% between Korean and Japanese strains. The comparison between S. miscanthi and S. celarius showed that ITS2 and COI differed by 7.2% and 7.9%, respectively. In addition, species-specific primer sets of both species were designed to show the species classification within the genus.