The purpose of this paper is to trace the architectural origin of the Bosung College Library (1935~37), which is currently used as Graduate School Building of Korea University. So far, numerous books have repeatedly described that the library was modelled on a Duke University library, but without any serious consideration. Through literature review, field-trip and archives investigation, this research discovered new critical facts concerning the origin of the building. First, Dong-Jin Park, the architect for Bosung College, saw a photograph of the Duke library in a Duke University catalog possessed by Chun Suk Auh, Professor of the college at that time. Second, the Duke library that he saw in the catalog, which might possibly be Bulletin of Duke University (Feb. 1931), is certainly the present Perkins Library (1930) in Duke University West Campus. Third, the architect probably referred not only to the library but also to other Duke buildings such as School of Medicine and The Union, of which photographs were also published impressively in the Bulletin. Although the Bosung College Library was inspired by the Duke buildings, however, it is undoubtedly a creative design work by the architect Park. Arguably, these findings broaden our view of Korean architectural history in the modernization period, and it is more than a confirmation of just one building's origin.

The gene encoding an esterase enzyme was cloned from a metagenomic library of cow rumen bacteria. The esterase gene (est2R) was 2,120 bp in length, encoding a protein of 516 amino acid residues with a calculated molecular weight of 57,286 Da. The molecular weight of the enzyme was estimated to be 57,000 Da by SDS-PAGE. Est2R shared 35.6% amino acid identity with esterase (CAH19079) of uncultured prokaryote. The Est2R was most active at 20-40°C, and showed optimum at 30°C and pH 8.0. The most activity of Est2R for the different chain length of p-nitrophenyl ester group as substrate was p-nitrophenyl acetate. Moreover, the enzyme was found to be most active without organic solvent, followed by 98% active with ethanol, and the enzyme activity was highly affected by the acetonitrile. The enzyme was significantly inhibited by Zn2+ but stimulated by Ca2+. So, novel esterase gene est2R is likely to obtain from cow rumen metagenome and supposed to use for industrial purpose.

We tested the identification ability of DNA barcodes comparing with morphological data using the Korean butterflies. The 921 samples (4.6 samples per species) for 202 resident Korean species except migratory species were used. The obtained samples were morphologically identified based on wing patterns. In a result, genetic divergence to the nearest-neighbouring taxon varied from 0 to 28.2%, with an average of 13.4 per cent. The neighbour joining (NJ) tree profile showed that sequence data for 185 of the 202 species formed distinct barcode clusters. Thus, our results indicated that 91.6 percent of the species were possible to allow the reliable identification using DNA barcoding. The rest 17 species (8.4%) consist of following four cases: clustering separated from each species by less than 1% branch length (two species pairs), paraphyletic clustering (two species pairs and one triple species pair), polyphyletic clustering with sharing barcodes (three species pairs), and clustering separated from existing species by the deep branch divergence (four clusters). However, it was not easy to interpret these ambiguous cases only using our current taxonomic evidences. Therefore, we are performing integrative taxonomy on these cases using other additional evidences such as examination on male genitalia and analysis of other gene regions.

국화과(Compositae)는 현화식물 중 세계에서 가장 넓게 분포하고, 쌍자엽식물 중 가장 진화된 식물분류군이며, 우리나라에는 약 300여종이 존재하는 것으로 알려져 있다. 구절초, 감국, 쑥, 쑥갓, 개미취, 참취, 곰취 등 국화과 식물들은 예로부터 민간에서 약용 및 식용 소재로써 다양하게 사용되어왔다. 본 연구는 국화 및 국화근연종 유용유전자원 선발을 통하여 육종 소재를 확대하고, 중간모본 및 신품종 육성기반을 구축하고자 DNA 마커시스템의 개발을 위해 수행되었다. 1. 화단국인 Smileball(Dendranthema grandiflorum) 품종을 사용하여 SSR-enriched library를 작성하였고, GS FLX 분석을 통해 18.83Mbp의 염기서열 결과를 얻었으며, read의 평균 길이는 280.06bp로 나타났다. 2. 단순반복염기서열(SSR) 부위를 포함하는 26,780개 clones 중 di-nucleotide motifs가 16,375개(61.5%)로 우세하였고, trinucleotide motifs(6,616개, 24.8%), tetra-nucleotide motifs(1,674개, 6.3%), penta-nucleotide motifs(1,283개, 4.8%), hexa-nucleotide motifs(693개, 2.6%) 순으로 나타났다. 3. 얻어진 di-nucleotide motifs들 중에서는, AC/CA class가 93.5%로 대부분이었고, tri-nucleotide motifs에서는 AAC class가 50.5%, tetra-nucleotide motifs는 ACGT class가 43.6%이고, pentanucleotide motif에서는 AACGT class 27.2%이며, hexa-nucleotide motif에서는 ACGATG class 21.8%였다. 4. 얻어진 염기서열 결과를 토대로 다양한 motif를 갖는 100개의 SSR 마커를 제작하였고, 차후 이를 활용하여 국화 유전자원의 다형성 및 유전자형 분석을 통해 분자유전학적 다양성 및 집단의 구조분석이 가능하고, 국화의 분자육종기반 구축을 위한 유용한 도구가 될 것 이다.

Antimicrobial peptides are widely found in living organisms and are known to play a critical role in innate immunity. Numerous antimicrobial peptides from diverse species appear to be effective against pathogenic microorganisms of bacteria, fungi, protozoa, and viruses. Because antibiotic resistance is a global health issue in the fight against pathogenic microorganisms, there has been an urgent need for development of new antibiotic substances. In the current study, we performed yeast two hybrid screening using Beclin1 bait in order to find new peptide antibiotics from a random peptide library. Two candidate peptides from the screening were expressed in a yeast secretory system of Pichia pastoris and tested for any antimicrobial activity against Staphylococcus aureus, MRSA, MRSA2242, MRSA2250, Lactobacillus casei, and Lactobacillus acidophilus. Disc clear zone assay and spectrophotometric analysis revealed that the two peptides exert a decent activity against the pathogenic bacteria, in contrast to minimal effect on the commensal Lactobacillus strains. Taken together, this study presents novel peptides with antibacterial activity against the pathogenic forms of Staphylococcus aureus and suggests the possibility that these peptides, upon further characterization, may be developed as clinically useful antibiotics.

머루(Vitis coignetiae)는 국내에 자생하는 야생종 포도의 한 종류로서, 포도 새눈무늬병에 저항성이다. 내병성 포도 육종에 활용할 유용한 육종소재를 개발하고자 머루의 잎과 과실로부터 cDNA libraray를 제작하였다. 머루의 cDNA library의 총 5,760개의 EST 클론을 분석하여 676개의 contig와 2,306개의 singleton을 분리하여 총 2,982개의 unigene을 분리하였다. NCBI 데이터베이스의 BLAST를 통한 상동성을 검색한 결과, 2,241개의 클론이 기능이 알려진 유전자이었고, 그 중에서 1,442개는 생물학적인 대사에 관여하고 836개는 세포구성물과 관련이 있었다. EST와 contig의 평균 크기는 각각 702bp와 757bp이었다. 포도새눈무늬병균에 감염된 머루 cDNA library로부터 Proline-rich cell wall protein, thaumatin-like protein, class IV chitinase, and pathogenesis-related (PR) protein 10 등의 다양한 방어관련 유전자와 광합성관련유전자 및 수분스트레스 저항성 관련유전자가 많이 검출되었다. 본 연구를 통해 얻어진 머루의 EST 자료는 포도의 새로운 유전자원에 관한 연구 및 내병성 포도 신품종 육종 프로그램에 기본자료로 유용하게 활용될 것이다.

Rhynchium brunneum is a widely distributed wasp species in South Eastern Asia. R. brunneum females were collected from rural provinces of Cambodia, and their total RNA and venom were extracted on site. To search for novel substances in venom, a subtracted cDNA library specific to the venom gland and sac was constructed. A total of 1118 expressed sequenced sequence tags (ESTs) were sequenced and assembled into 349 contigs (107 multiple sequences and 242 singletons). In this result, we found the putative neurotoxin (DTX protein precursor), antimicrobial peptides (teratocyte-specific caboxylesterase) together with typical major components of wasp venom (venom hyaluronidase, arginine kinase, phospholipase A2, serine/theonine protein phosphatase). Additional in-depth annotation would be required for further characterization of many unidentified genes found in the EST library.

We present medium resolution (R = 5000 - 6000) spectra in the near-infrared band, 1.4 - 1.8 μm, for template stars in G, K, and M types observed by the echelle spectrometer, IRCS, at the SUBARU 8.2 m telescope. The identification of lines is based on the spectra of Arcturus (K2 III) in the literature. We measured the equivalent of widths and compared our results to those of Meyer et al. (1998). We conclude that our spectral resolution (R = 6000) data can investigate more accurately the properties of lines in stellar spectra. The library of the template stellar spectra in ASCII format are available for download on the World Wide Web.

There are an estimated about 20,000 species of mushrooms in the world. Of these, approx. 2,0000 species is edible and around 1,500 species of them grow naturally in Korea. About 300 species of Korean natural mushroom could be classified as edible and medical purpose, but actual use is restricted to only 20 species. Mushrooms has been used for food ingredients since early times. Recently it is being used as a good pharmaceutical material or functional bio-material. Especially Ganoderma lucidum, Phellinus and Inonotus are used for medicinal purposes. In this meaning, the study about chemical component separation and active component effect is necessary. For the study, the systematic establishment of mushroom should be done. In this study, we are trying to build a library by using extracts from the edible, medicinal and wild mushrooms. The physiological activities(antioxidant, anticancer and anti-inflammatory effects) were performed to investigate the effectiveness of mushrooms.

본 논문은 휴대 전화를 이용하여 근거리 무선 통신을 수행할 때 발생하는 과금, 지연, 가입된 이동통신사가 다른 단말 간 통신 등의 제약을 해결하기 위한 방법으로, 블루투스(Bluetooth) 기반의 외부 통신 장치를 제안한다. 제안하는 방법을 통해 블루투스가 내장되어 있지 않은 단말기, 블루투스가 내장되어 있다 하더라도 이를 제어할 수 없는 단말기에서도 근거리 무선 통신을 지원할 수 있게 된다. 또한 WIPI Jlet 기반의 소프트웨어 제작을 통해 제안한 하드웨어를 활용하는 여러 가지 방법에 대해 기술한다. 마지막으로 제작된 외부 통신 장치와 라이브러리를 이용하여 두 개의 게임을 실제로 만들었다. 이러한 게임은 사용자들이 근거리 통신을 통하여 게임을 진행하는 방식이다. 이러한 게임들을 통하여 일반 기능폰 간의 통신뿐만 아니라 기능 폰과 Windows Mobile과 같은 스마트폰과의 통신을 통한 다양한 활용성에 대해 기술한다.

The aim of this study is to estimate and compare indoor air quality(IAQ) at stock rooms in general library and in National Archives and to provide fundamental data in order to enact the law on management of IAQ at stock rooms in general library. The survey of IAQ in stock rooms was performed in Seoul National Archives in August 2009, and the basic data on level of indoor air pollutants in stock rooms at general library was collected from domestic journal. The concentration of NO₂ was 114.1 ppb at audiovisual documentary stock rooms in National Archives. That value exceeded 50 ppb guideline that was legislated by the Korean Ministry of Public Administration and Security. The concentration of PM₁₀ and VOCs were 59±9㎍/m³ and 367±83㎍/m³, respectively at stock rooms in general library. These values were exceeded from the guideline of Korean Ministry of Public Administration and Security that is 50㎍/m³ for PM₁₀ and 400㎍/m³ for VOCs. Also, the exceeded probability from the guideline was 83.7% in PM₁₀ and 35.6% in VOCs. Therefore, we suggest that a national plan for the management of IAQ at stock rooms in general library should be established.

본 연구는 평택시립안중도서관과 지역 주민의 적극적 참여로 이루어진 정보 서비스, 독서 및 문화 프로그램 추진 사례를 통해 지역 공동체 중심 공간으로써 공공도서관과 사서의 역할을 되짚어보고 시민들과 함께 추진한 공공도서관 프로그램의 세 가지 방향을 중심으로 지역 도서관 프로그램의 서비스 방향을 제시하고 있다.

Recently Korean government introduced and strengthened the environmental polices to manage and enhance indoor air quality as a major assignment. As a result,“Indoor Air Quality Management Act”has been activated since May 2004. However, among many multi-usage facilities, the school environment has been excluded from such regulations. Thus, the purpose of this study is to survey indoor aerosol and CO2 concentrations in a university library which is one of major school facilities. Concentrations of PM10, PM2.5, and PM1 were intensively measured every 5 minute interval by means of a dust monitor with light-scattering principle. The mini-volume air samplers were used further to analyze inorganic elemental compositions of PM10 and to inter-compare with the dust monitor. Carbon dioxide was simultaneously monitored by a multi-gas monitor equipped with a non-dispersive infrared system. PM10 concentrations did not exceed the indoor air quality standard(150㎍/㎥) for all the sampling places. However, quick and steep increases of aerosol concentration were observed during the Asian Dust storm period. In addition, the concentration of PM10(74.1㎍/㎥) in the main university office was the highest among the entire sampling places. Although there was no specific pollution source in the library, the levels of aerosol and CO2 increased quickly due to various outdoor sources and human activities. The average CO2 concentration measured in three reading rooms, where ventilation was not fully operated, exceeded the indoor air quality standard of 1,000 ppm. Based on these case studies, effective management and reduction plans for indoor aerosol and CO2 were suggested for the large scale public buildings including university libraries.

이미지의 유사성을 결정하는 요인을 톤(tone), 즉 명도와 채도로 결정하여 정보 엔트로피를 계산하여 상관 계수를 계산하였다. 이미지의 톤을 알아보기 위해 영상정보의 색 공간을 RGB color space에서 HSI color space로 전환하였다. 이후 유사성을 판단하기 위해 이미지의 전체 픽셀수가 아닌 엔트로피 값의 범위에 따라 전체 70%의 픽셀 또는 이미지를 가장 많이 구성하는 세 가지 톤의 픽셀 수에 의해 결정되었다. 'Romantic'이라는 인간의 감성으로 판단된 18개의 영상정보를 선정하여 위의 모델을 적용, 이미지 유사성을 판단하였다.