본 연구는 국내에서 생산되거나 해외에서 수입되어 국내에서 유통되는 수산물 중에서 두족류를 문어류, 낙지류, 오징어류, 주꾸미류, 꼴뚜기류의 5개 그룹으로 구분하여 분석하였다. 두족류 5개 그룹을 판별을 하기 위해 미토콘드리아에 존재하는 유전자를 분석하였고, 그 중에서 COI (mitochondrial cytochrome C oxidase subunit I), 16s rRNA (16s ribosomal RNA), 12s rRNA (12s ribosomal RNA) 내에서 상당히 유사한 DNA 서열 부분과 일부 서열 변화 부분이 확인되었다. 명확하게 두족류 5개 그룹 판별을 하기 위해 COI, 16s rRNA, 12s rRNA 유전자의 일부 서열 변화 부분에서 그룹 특이적 프라이머 세트를 디자인하였다. 국내·외에서 확보한 두족류 시료(참문어, 낙지, 살오징어, 아메리카 대왕오징어, 갑오징어, 주꾸미, 모래주꾸미, 하이야주꾸미, 참꼴뚜기, 창꼴뚜기, 한치꼴뚜기)의 genomic DNA을 추출하여 각 그룹의 특이적 프라이머를 이용하여 SYBR 기반의 real-time PCR 시스템에 의해 분석되었고, threshold cycle (Ct) value와 같은 real-time PCR 결과 분석에 의해 두족류 내 그룹 판별이 가능하였다(Table 3).

Squid is one of the important fisheries resources in Korea. Therefore, squid has been designated and managed as a target species of total allowable catch (TAC) since 2007, but the catch amount is gradually decreasing. The analysis was conducted to identify the change of relative fishing power index to develop the vessel and gear technology that may have improved the fishing efficiency of the offshore squid jigging fishery from 1960s to 2010s. Gross tonnage per fishing vessel increased with the increase in size until 1990, but then gradually decreased to 41.0 tons in 2000 and 37.1 tons in 2010. The illuminating power (energy consumption) by fishing lamps increased to 180 kW in 2005 and stabilized to 120 kW in 2015. Jigging machine started to be supplied to fishing vessels from the early 1970s, and fish finders began to be supplied in the early 1980s and gradually increased. Therefore, the relative fishing power index in the offshore squid jigging fishery increased from 1.0 in 1980 to 1.1 in 1990, to 3.5 in 2000 and to 2.5 in 2010, but the increment rate slowed down gradually. The results are expected to contribute to reasonable fisheries stock management.

A study for species composition and abundance of the fish assemblages in the coastal Sacheon Bay, Korea were conducted using monthly collection by a shrimp beam trawl from March 2015 to February 2016. The number of individuals, biomass and species diversity indices fluctuated with the station and month. Of all 73 species collected, the dominant species in terms of numbers were Liparis tanakae, Zoarces gillii, Amblychaeturichthys hexanema, Ricuzenius pinetorum and Pleuronichys cornutus. The peak number of fishes occurred in March and April, whereas the biomass of fishes was highest in April. The numbers and biomass of fishes were lower in February corresponding with the low temperature, and diversity indices were highest in June. Due to those seasonal difference in abundance of the dominant species, fish community were divided into three main groups. In addition, seasonal difference by station of fish community were devided into four main groups. Juvenile fishes of coastal Sacheon were presented during the study period. It presented that the study area served as an important role in spawning and nursery ground for fisheries resource.

The aim of the present work was to develop simultaneous methods of quantification of carazolol, azaperone, and azaperol residues in livestock and fishery products using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Samples were extracted from beef, pork, chicken, egg, milk and shrimp using acetonitrile (ACN); while flat fish and eel were extracted using 80% ACN. For purification, ACN saturated n-hexane was used to remove fat composition. The standard calibration curves showed good linearity as correlation coefficients; r 2 was > 0.99. Average recoveries expressed were within the range of 67.9-105% for samples fortified at three different levels (0.5 × MRL, 1 × MRL and 2 × MRL). The correlation coefficient expressed as precision was within the range of 0.55- 7.93%. The limit of quantification (LOQ) was 0.0002-0.002 mg/kg. The proposed analytical method showed high accuracy and acceptable sensitivity based on Codex guideline requirements (CAC/GL71-2009). This method can be used to analyze the residue of carazolol, azaperone, and azaperol in livestock and fishery products.

본 연구는 울릉도 수산자원 감소 문제를 해결하기 위한 방안 중 하나로서 정치성 구획어업의 도입을 위한 시험 연구를 수행하였다. 시험용 어구인 삼각망은 울릉도 해역의 환경, 운영 및 관리 비용, 소형 어선 및 인력이 운영할 수 있는 조건을 고려하여 선정하였다. 2017년 4월부터 5월까지 삼각망을 이용하여 11회 어획 조사한 결과, 총 개체수는 2,735개체, 중량은 983.4 kg 으로 계측되었으며, 우점종은 참돔, 방어, 넙치, 한치, 전갱이, 쥐치류 등으로 조사되었다. 조사 기간 중 유용 수산자원은 어류의 경우 조피볼락(Sebastes schlegelii), 볼락(S. inermis), 개볼락(S. pachycephalus), 쑤기미(Inimicus japonicus), 노래미(Hexagrammos agrammus), 농어목 어류 붉 바리(Epinephelus akaara), 참돔(Pagrus major) 그리고 말쥐치(Thamnaconus modestus)로 분류 되었으며, 두족류인 한치(Uroteuthis chinensis)의 어획량이 많은 것으로 분석되었다. 울릉도의 주요 수산자원 중 하나인 살오징어의 생산량이 지속적으로 감소할 경우, 삼각망과 같은 소형 정치성 구획어업의 도입은 어민 수입 창출의 대체 수단으로 유용할 것으로 판단된다.

Pacific bluefin tuna (Thunnus orientalis) has been mostly caught by the Korean offshore large purse seine fishery in Korean waters. The annual catch of Pacific bluefin tuna caught by the offshore large purse seine fishery in Korean waters showed less than 1,000 mt until the 1990s except for 1997. The catch sharply increased to 2,401 mt in 2000 and recorded the highest of 2,601 mt in 2003, but the catch has generally decreased with a fluctuation thereafter. The main fishing ground of Pacific bluefin tuna of this fishery is formed around Jeju Island. However, it expanded to the Yellow Sea, the coastal of Busan, and the East Sea, which depends on the migration patterns of Pacific bluefin tuna by season. The CPUE standardization of Pacific bluefin tuna was conducted using Generalized Linear Model (GLM) to assess the proxy of the abundance index. The data used for the GLM were catch (weight), effort (number of hauls), catch ratio of Pacific bluefin tuna, moon phase by year, quarter and area. The standardized CPUE from 2004 to 2011, except for 2003 and 2010, showed a steady trend, and then increased until 2014. The CPUE in 2015 decreased, and in 2016 was higher than that in 2015. The result of GLM suggests that the effect of the catch ratio of Pacific bluefin tuna is the largest factor affecting the nominal CPUE.

본 연구에서는 우리나라 식품공전에서 불검출 물질로 관리하고 있는 니트로빈(nitrovin)에 대해 고감도 정량·정성 분석이 가능한 LC-MS/MS를 적용하여 적합한 분석법을 제시하고자 하였다. 수산물 시료는 아세토니트릴/물로 추 출하고 아세토니트릴 포화 헥산으로 지방을 제거하여 고상추출 카트리지를 적용하여 정제하였다. 분석물질은 전기분무이온화방법의 positive mode에서 이온화하여 MRM 조건을 확립하여 분석하였다. 개선된 시험법은 CODEX CAC/GL-71 가이드라인에 따라서 정확성, 정밀성, 직선성, 정량한계에 대한 검증을 통하여 유효성을 확인하였다. 본 실험에서의 정량한계는 0.001 mg/kg 수준이며, 정량한계를 포함하는 표준시료에서 얻어진 검량선의 상관계수(r2)는 0.985 이상으로 시험법의 직선성이 유효함을 판단할 수 있었다. 또한, 수산물(넙치, 장어 및 새우) 시료에 대한 니트 로빈의 평균 회수율과 변동 계수는 72.1~122%, 2.9~16.9% 로 확인되어 정확성 및 정밀성이 CODEX가이드라인에 부합하였다. 따라서, 개선된 니트로빈 정량분석법은 수산물 중 니트로빈을 분석하는데 적합하며, 니트로빈에 대한 지속적인 잔류실태조사에 활용되어 수산물 중 니트로빈의 안전관리에 기여할 것으로 판단된다.

본 연구는 국내 생산 및 수입 양식 수산물에 대해 잔류 할 수 있는 향정신성 의약품인 디아제팜 대한 안전관리 강화기반을 위해 마련되었다. 중국인민공화국 국가 표준 시험법(GB 29697-2013)을 기반으로 전처리 방법을 개선 하여 GC-MS/MS 시험법을 확립하였으며, LC-MS/MS 방 법과의 기기간 검증을 통해 확립된 시험법의 선택성, 정량한계 및 회수율에 대한 검증을 통해 디아제팜 시험법으로서의 유효성을 확인하였다. LC-MS/MS의 경우 아세토니트릴로 추출 후 PSA를 이용해 정제하였고, GC-MS/MS의 경우 아세토니트릴로 추 출후 C18카트리지를 이용해 정제하였다. 디아제팜은 표준용액을 정량한계를 포함한 농도에 따라 검량선을 작성한 결과 두 기기 모두 r2> 0.99 이상의 직선성을 확인하였다. 본 실험에서의 검출한계와 정량한계는 LC-MS/MS 및 GCMS/ MS 모두 0.0004 mg/kg, 0.001 mg/kg 수준이었으며, 평균 회수율은 각각 99.8~106%, 109~124%이었다. 또한, 분석오차는 모두 15% 이하로 정확성 및 재현성이 우수하였 으며, CODEX 가이드라인 규정에 만족하는 수준이었다. 따라서 개발된 시험법은 안전한 수산물의 국내 유통과 잔 류실태조사를 위해 활용될 것으로 기대한다.

The aim of this study is to investigate influence of moon light to the fishing of squid jigging fishery in the southwest Atlantic Ocean based on analyses of date taken from 127 vessels in Falkland fishing ground by squid jigging fishery from 2010 to 2015. Catch and CPUE were analyzed between the new moon and full moon phases. Catches of the new moon phase were higher than those of the full moon phase by 7.6% and CPUE expressed in mt/day-vessels and mt/line-day were also higher by 18.2%, 18.2% respectively. However, as a result of statistical analysis at a significance level of p ＞ 0.05, no significant statistical differences in catch, mt/day-vessels and mt/line-day were found between the new moon and the full moon as a result of statistical analysis at a significance level of p ＞ 0.05.

In this study, the differences of holding power according to the shape and weight distribution of concrete weight used in shellfish shell fishery were investigated through the experiments. To investigate the differences in shape, five bar-shaped concrete weights with the same length and different cross-sectional shapes were produced. The sectional shape of each weight was square, triangle, circle, small cross, and large cross (SQ, TR, CI, CR-S, CR-L). Ten rectangular parallelepiped weights with different bottom area and cross-sectional area were produced. To investigate the differences by the weight distribution, the holding power on the square model (SQ) with six 50 g weights at different positions was investigated. All the holding power was obtained by measuring the tensile force generated when the concrete weight was pulled at a constant speed on the sand. As a result, there were no differences in holding power between the ten rectangular weights. However, the experiment on weights with different cross-sectional shapes showed differences in holding power. The holding power was higher in the order of CR-L > CR-S > CI > TR > SQ. In the weight distribution test, the holding power was higher as the front side of the weight was heavier. Generally, the frictional force is the same even if the shape is different, when two objects have the same value in the weight and the roughness. On the other hand, it seems to have a large impact when the shape of the bottom is deformed in the course of pulling the object. Particularly, the larger the degree of protrusion like cruciform weights, the more the holding power increased while deeply digging the bottom. It is also likely that the holding power increases as the front weight increases.

Although the main objective of Free Trade Agreements (FTA) is market integration among member countries, there are limited studies supporting this impact. Our study explores whether FTA has enhanced market integration between South Korea and its FTA partners, focusing on South Korea’s fishery product import market. We investigate two research questions concerning FTA impacts: first, whether trade costs declined when South Korea imported fishery products from its FTA partners after the FTA; second, if the speed of the convergence of South Korea-its FTA partners’price differential of imported fishery products on trade costs result to occur more quickly after the FTA. To determine these outcomes, we utilize a Threshold Autoregressive Model covering the sample periods from January 2002 to April 2017. Our findings demonstrate the effects of FTA on market integration are different among FTA partners. FTA has enhanced the market integration between South Korea and Norway, Vietnam, and Spain, respectively, but not for others. Therefore, we find positive evidence of FTA on fishery import market integration between South Korea and Norway, Vietnam and Spain, respectively.

Fishing dredge in Jeollabuk-do began to become widespread in the 1960s and has continued to catch diverse kinds of shellfish in the productive fishing grounds around Dongjin River, Mangyeong River and Geum River estuaries. Since the 1970s, the construction of various large-scale industrial complex and the implementation of Saemangeum reclamation project have resulted in a decrease in main fishing areas and a sharp decline in shellfish production. As a result, dredge fishery has faced many difficulties. Dredge fishery in Jeollabuk-do is carried out with a total of 30 fishing permits as of 2016. Surf clams, hen clams, bladder moon snails, and common orient clams were mainly caught before the construction of Saemangeum dike while comb pen shells, purple whelks and ark shells are mainly caught afterwards. Inside the Saemangeum dike, most fish species have disappeared due to low water level and low salinity, and littleneck clams are caught using a jet pump type of fishing dredge. Outside the dike, the diversity of shellfish species has been reduced; comb pen shells are mainly caught. In this process, a lot of friction occurs due to the use of a reformatted dredge. Therefore, a lot of research needs to be conducted in the near future.