This study was peformed to investigate effect of feeding experimental mixture containing chitosan, hibiscus extract and L-carnitine on body weight and lipid metabolism in rats. Forty-eight male rats(Charles River CD) of eight weeks old and weighing 336.5±2.3g were raised for five weeks with high fat diet(40% fat as calorie) to induce obesity. After induction of obesity, rats weighing 560.4±5.6g were blocked into four groups according to body weight and raised for eight weeks with diet containing either 0.09%(+1D group), 0.9%(+10D group) or 4.5%(+50D group) of experimental mixture. Aspartate aminotransferase(AST) and alanine aminotransferase(ALT), total protein and albumin were normal levels in plasma. Body weight gain and epididymal fat pad weight were lower in experimental mixture groups than control group However, weights of perirenal fat pad and brown adipose tissue were not significantly different among all groups. There was no significant difference in plasma and hepatic lipid levels among all groups. Liver citrate lyase and camitine acyltransferase activities were not significantly different among all groups, however, citrate lyase activity was tended to be decreased with increasing experimental mixture level in diet. Fecal total lipid and total cholesterol excretions were highest in +50D group, and triglyceride excretion was highest in +1D group. in conclusion, intake of experimental mixture containing chitosan, hibiscus extract and L-camitine was effective in reducing body weight and body fat, and its inhibitory effects might lead to obesity improvement.

Extraction of Sedum sarmentosum Bunge by super critical carbon dioxide was operated under 40-50℃and 200-250 atm, thus, yield of extraction was very low as 4 wt%. Rats were administrated with the extract orally once a day for succesive 6 days, followed by treatment with carbon tetrachloride (CCl4) on the sixth day. The activities of aminotransferase, alkaline phosphatase, γ-glutamyl transpeptidase, lactate dehydrogenase and contents of triglyceride, total cholesterol in the extract-pretreated rats were decreased compared to the CCl4controled rats, whereas content of HDL-cholesterol was increased. Especially content of hepatic malondialdehyde (MDA) and atherogenic index (AI) were decreased and HTR was increased in the extract-pretreated rats, and reduced peroxidative liver damage in the CCl4-induced hepatotoxicity rats. In addition, activities of hepatic superoxide dismutase, catalase, glutathione peroxidase in the extract-pretreated rats were significantly decreased compared to the CCl4 controled rats, but the content of glutathione was significantly increased. These results suggest that extract of Sedum sarmentosum Bunge has hepatoprotective effect in the CCl4-intoxicated rats.

다시마 분말 첨가식이가 당뇨쥐의 당질과 지질대사 및 항산화효소계에 미치는 영향을 규명하기 위하여 SD계 횐쥐에 17% 다시마 분말을 급여하여 7주간 실험 사육한 후 혈청의 포도당 및 지질농도와 주요장기의 항산화효소의 활성도를 관찰하였다. 다시마 당뇨군(TI)에서 다사마 섭취는 당뇨에 의한 체중감소 현상을 보였다. 당뇨군의 혈당 농도는 다시마 섭취에 의한 혈당강하 효과는 3주째에 나타났다. 당뇨에 의한 산화적 스트레스로 인해 증가한 GST 활성도가 다시마 섭취로 인해 정상 수준으로 저하됨으로써 다시마의 섭취가 당뇨시 산화적 반응에 대한 방어기전을 제공할 수 있음을 유추할 수 있었다. 또한 중성지방이 감소하고 HDL-콜레스테롤의 증가하는 경향으로 심혈관계와 관련되는 당뇨합병증의 억제 가능성을 제시하였다.

The effect of treatment with Citrus junos fractions (citron 3W, citron 3H, citron 4W and citron 4H) upon rat hepatocytes exposed to alcohol was investigated. We compared the serum biochemistry of rats administered both alcohol and Citrus junos fractions t

In order to investigate whether or not the alcohol-treated rat liver cells can be protected by Korean Citrus junos and medicinal herbs, We compared the serum biochemistry of rats administered both alcohol and the complex of Korean Citrus junos and medicin

This study was performed to investigate the effect of Lycii fructus beer on serum lipid profiles and antioxidant activity in rat Sprague-Dawley (SD) rats weighting about 190g were divided into the following 5 groups ; distillate water (Control), 5% ethanol in distillate water (Ethanol), commercial beer (CB), Lycii fructus beer (LFB) and 5% alcohol red wine diluted with distillate water (RW). Body weight, total food intake, FER and percent organ (liver, kidney) weight per body weight were not significantly changed by Lycii fructus beer drinking. After 6 weeks, serum total cholesterol, triglyceride and HDL cholesterol level were not significantly different. But, Lycii fructus beer intake tended to decrease serum triglyceride level and atherogenic index. Also, GOT and GPT levels were expressed lower than Ethanol group. There was not significantly different in hepatic glutatiione (GSH) content and glutathione-S-transferase (GST) activities among 5 groups. Lipid peroxidation in the hepatic was decreased by Lycii fructus beer intake. The results demonstrated that Lycii fructus beer was potential and effective antioxidant that can protect the decrease associated with alcohol.

This study was performed to investigate nutritional intake, the dietary behaviors and plasma lipid profile between smokers and non-smokers of college male students in the Gyeonggi Area. Dietary behaviors and attitude toward smoking were investigated by questionnaires. The result obtained were as follows: There was no significant difference in anthropometric measurements between smokers and nonsmokers. There was no significant difference in dietary behaviors. However, smokers tended to eat more snacks and desserts than nonsmokers. In plasma lipid levels, smokers had higher levels of plasma triglyceride, LDL-cholesterol, VLDL-cholesterol and total cholesterol than that of non-smokers(p<0.05). HDL-cholesterol level of smokers was a lower than that of non-smokers although the difference was not significant. AI(Atherogenic Index), Total /HDL cholesterol ratio and LDL/HDL cholesterol ratio of smokers were significantly higher than that of non-smokers(p<0.01). BMI and SBP showed positive correlation with triglyceride(p<0.01) and VLDL-cholesterol(p<0.01). DBP showed positive correlation with triglyceride(p<0.001) and VLDL-cholesterol(p<0.01).

삼백초가 TCDD 투여로 유발된 rat의 지질대사에 미치는 효과를 알아보기 위해 NTT, TTA, STT군으로 각각 10마리씩 나누어 TCDD를 투여하고 4주 동안 물질을 투여하였다. 4주 후 희생시켜서 생체 내 간 조직 활성 효과 및 항산화 효과를 관찰한 결과는 다음과 같다. 1. Total Cholesterol에서 삼백초를 투여한 STT군은 NTT군 비교해서 14.46% 증가하였으며, 혈청중의 HDL-Cholesterol은 SST군이 TTA군과 비교해서 21.29% 증가하였고 94.98%로 억제되었다. LDL-Cholesterol에서는 STT군이 TTA군 보다 12.86% 감소하였으며, 48.34% 로 억제되었다. 2. 혈청중의 TG의 함량은 TTA군이 NTT군에 비해 34.84% 증가하였다. TTA군에 비해 STT군은 7.06% 감소하였으며, 18.9% 억제시킨다. 3. 간조직중의 MDA량은 STT군이 TTA군에 비해 17.14% 감소하였고, 32.72%의 억제율을 보였다.

The effects of dried green tea leaf powders on serum lipid concentrations were evaluated in rats. Sixty male Sprague-Dawley rats weighing 100±10g were divided into six groups and fed high fat diets for six weeks. Experimental groups were administered with following diets; Normal fat diet and normal and high fat diets with 1% dried green tealeaf powders. Tissue weights of liver, lung, stomach, heart, kidney and spleen of high fat diet exposed rats were reduced by dried green tea leaf powders groups. The concentrations of serum triglyceride in rats fed the dried green tea leaf powders were lower than those in other groups. The concentrations of total cholesterol in green tea leaf powders the were lower than those in high fat diet groups. The concentrations of HDL-cholesterol in serum of the dried leaf powders green tea were significantly higher than those of other groups. The levels of LDL-cholesterol in serum of the dried green tea leaf powders groups were tended to be lower than those of other groups. GPT and GOT were decreased in dried green tea leaf powders groups and than in the high fat group. LDHase was lower in the dried green tea leaf powders groups than in the high fat group. These results suggest that dried leaf powders green tea groups may reduce elevated levels of serum lipid concentrations in rats fed high fat diets.

참치스프레드에 양파즙을 5%, 15%, 30% 및 45%씩을 첨가하여 냉장 저장 시 참치스프레드의 품질 특성 및 지질 산패도를 측정함으로써 양파의 천연항산화제로서의 효용성을 검토하였다. pH는 전반적으로 저장 초기에는 pH가 6.06~6.20정도이나 시간이 경과함에 따라 서서히 감소하다가 21일 이후 다시 증가하는 경향을 보였다. 대조구는 21일 이후 급격한 증가를 보이다가 저장 49일까지 증가되었고 양파즙 첨가구는 서서히 증가하다가 저장 42일 이후 감소하는 경향을 보였는데 양파즙 15%와 양파즙 30% 첨가구의 경우 증가폭이 다소 완만하였다. 산가는 초기에 0.68~0.78이었으며 저장기간이 경과함에 따라 모든 실험구에서 유의적으로 증가하였으나 양파즙 15%와 30% 첨가구에서 가장 낮게 나타나 지질의 산화 억제에 매우 효과적이었다. 과산화물가는 초기에 2.2~2.6 meq/kg이었으나 저장기간이 경과함에 따라 유의적으로 증가하여 냉장 14일까지는 약간 증가하다가 그 후 급격히 증가하였고, 대조구는 지속적으로 증가하여 저장 42일에는 50.57 meq/kg로 가장 높은 과산화물가를 나타내다가 그 후에는 감소하였다. 양파즙 첨가구는 대조구에 비해 상당히 안정한 효과를 나타냈으나 42일 이후부터는 감소하는 경향을 보였다. 양파즙을 첨가한 참치스프레드의 TBA가가 전반적으로 대조구에 비해 낮아 양파가 지방의 산화를 지연시켰음을 알 수 있었고, 양파즙 첨가량에서는 15%와 30% 첨가군이 5%나 45% 첨가군보다 낮은 TBA가를 나타내어 산가와 유사한 결과를 보였다. 색도에서 L값은 큰 변화를 나타내지 않았으나 대조구에 비해 양파즙 첨가구가 다소 높은 값을 보였고, 저장 전 보다 저장기간이 경과됨에 따라 L값은 증가하였다. 한편 a값은 저장기간이 경과함에 따라 감소하였고, 양파즙 5% 첨가구만 저장 35일 후 증가하는 경향을 나타내었으며, b값은 대조구와 첨가구 모두 저장 기간이 경과함에 따라 증가하였고, 양파즙 첨가구가 다소 높게 나타났다. 관능검사에서 맛은 저장 초기에 증가하다가, 저장 기간이 길어짐에 따라 감소하는 경향을 보였으며, 대체로 양파즙 15% 및 30% 첨가구가 높은 점수를 나타내 우수하게 평가되었다. 부패취도 양파즙 첨가량 및 저장기간에 따라 유의성이 인정되었고(p〈0.05), 양파즙 첨가구가 대조구보다 부패취가 적게 나타났으며, 양파즙 15% 첨가구에서 가장 좋게 평가되었다. 외관에 있어서 저장 28일까지 양파즙 15% 및 30% 첨가구가 가장 좋은 것으로 나타났으며, 대조구와 5% 첨가구는 비슷한 수준을 보였다. 경도는 저장기간 동안 감소되었지만 대체로 양파즙 15% 첨가구가 좋은 것으로 평가되었고, 전반적인 바람직성은 양파즙 15% 첨가한 참치스프레드에 대한 기호도가 가장 높았다.

본 연구는 여자 축구선수 25명을 대상으로 혈중 지질성분과 철분지표를 조사 .분석하고, 훈련기 동안의 철분보충이 혈청 지질과 지단백 콜레스테롤에 미치는 영향을 살펴보았다. 여자축구선수들의 평균연령은 23.3±2.5세였고, 평균 신장과 체중은 각각 164.4±5.7cm과 57.4±4.6kg이었고, 체지방율은 23.9±3.0%였다. 혈액 철분지표 분석결과 평균 적혈구 용적, 헤모글로빈, 총철결합력은 정상범위 내에 있었으나 낮은

본 연구는 미성숙, 성숙 단계의 돼지 난포란이 유리화 동결에 의한 동결보존이 가능한지를 조사 하고자 실시하였다. 난포란은 세포질 내 지방구를 분극시키기 위해 원심분리를 실시하였고, 미세조작기를 이용하여 지방구를 제거하였다. 돼지 난포란을 CB 처리하여 원심분리 후 지방구를 제거한 지방제거구(Delipated), CB 처리 후 원심분리만 하여 지방구를 분극시킨 원심분리구(Centrifuged), 아무처리도 하지 않은 대조구(Control)를 EM grid