The interest in greenhouse gases (GHG) emitted from all industries is emerging as a very important issue worldwide. This is affecting not only the global warming, but also the environmentally friendly competitiveness of the industry. The fisheries sector is increasingly interested in greenhouse gas emissions also due to the Paris Climate Agreement in 2015. Korean industry and government are also making a number of effort to reduce greenhouse gas emissions so far, but the effort to reduce GHG in the fishery sector is insufficient compared to other fields. Especially, the investigation on the GHG emissions from Korean fisheries did not carry out extensively. The studies on GHG emissions from Korean fishery are most likely dealt with the GHG emissions by fishery classification so far. However, the forthcoming research related to GHG emissions from fisheries is needed to evaluate the GHG emission level by species to prepare the adoption of Environmental labels and declarations (ISO 14020). The purpose of this research is to investigate which degree of GHG emitted to produce the species (swimming crab and snow crab) from various fisheries. Here, we calculated the GHG emission to produce the species from the fisheries using the life cycle assessment (LCA) method. The system boundary and input parameters for each process level are defined for LCA analysis. The fuel use coefficients of the fisheries for the species are also calculated according to the fuel type. The GHG emissions from sea activities by the fisheries will be dealt with. Furthermore, the GHG emissions for producing the unit weight species and annual production are calculated by fishery classification. The results will be helpful to establish the carbon footprint of seafood in Korea.

깨다시 꽃게(Ovalipes punctatus)는 갑각류로서 우리나라에서 잡히는 매끈 꽃겟속, 주름 꽃 겟속, 톱날 꽃겟속, 민 꽃겟속, 두갈래 민꽃겟속 들 중에 하나이다. 대부분의 꽃게는 가공되지 않은 상태로 찜 또는 찜육 등 반 가공 형태로 산업화 되었지만 최근에 게로부터 생리활성을 나타내는 펩타이드를 생산하는 연구가 발표되고 있다. 본 연구는 항산화 기능성을 나타내는 펩타이드를 선별하고 생산 최저공정 확립에 연구를 수행 하였다. 사용된 효소 alcalase, bromelain, flavourzyme, neutrase, papain, protamex들 중에서 bromelain으로 생산된 꽃게육 단백질 가수분해물이 가장 높은 활성을 보여 주었다. 꽃게육 단백질의 bromelain 가수분해물의 펩타이드들의 분자량 분포는 500-3,200 Da로서 7 종류의 이상의 펩타이드들로 구성되었다. 가수분해물의 구성아미노산 분포는 항산화 기능성에 관련된 소수성 아미노산은 전체 42.54%를 차지하였다. 가수분해물의 최적 생산 수율 조건을 확립하기 위하여 공정 조건, 효소 반응 온도 40-60℃, pH 6-8, 효소의 농도 1–3%(w/v)로 표면반응 분석법을 수행한 결과 효소 반응온도 55℃, 반응 pH 6.5, 효소의 양은 3%(w/v)에서 결정되었다. 최적 조건에서 단백질 가수분해도는 최대 71.60%에 도달하였다.

This study was conducted to investigate the attractant substances of different solvent fractions of mackerel and tuna viscera. Mackerel and tuna viscera were extracted with methanol and the resultant were fractionated with n-hexane, chloroform, ethyl acetate, n-butanol and water. The chemical compounds such as free amino acid, fatty acid, nucleotide related compounds contents were estimated for mackerel, tuna viscera and various fractions. These fractions were also subjected to attracting experiments in water tank to estimate attracting effects. The aqueous fractions of mackerel and tuna viscera showed the highest attraction effect on swimming crab. The major chemical compounds of the aqueous fractions were histidine, taurine, cysteine, glutamic acid, inosine monophosphate (IMP) and inosine (HxR) for mackerel and arginine, glutamic acid, aspartic aid, alanine, IMP and hypoxanthin (Hx). Results indicated that higher polarity compounds, such as amino acid and nucleotide related compounds have higher attraction activities than nonpolar substances such as neutral lipid.

To investigate the catching efficiency of a biodegradable round trap for a swimming crab (Portunus trituberculatus), four types of material (two types of biodegradable net, one type of biodegradable plus polyethylene net and one type of polyethylene net) for traps were used in the field test, and the field tests were carried out 11 times in the Boryeong fishing ground of Korea, 2012∼2013. The catching efficiency of four type traps was analyzed by catch per trap, weight per trap and mean weight per individual of small and large size swimming crab. Statistical Kruskal-Wallis test was carried out to verify the significant difference for the efficiency between types of material. The catch in number of swimming crab was 9,015 and formed about 94.3% of total catch. In the results of catching efficiency of traps, there was a little difference in catch per trap, weight per trap and mean weight per individual of swimming crab in-situ data. But there was no significant difference in catching efficiency between the biodegradable traps and the PE trap (P>0.05), so it’s needed to use widely a biodegradable trap for the conservation and sustainable management of swimming crab resources.

A series of fishing experiments were carried out in the western coastal waters of Korea from May, 2006 to September, 2007, using gill nets of different mesh sizes (m=101, 121.8, 152.5, 176.8, and 191 mm) to determine the mesh selectivity of gill net for swimming crab, Potunus trituberculatus. The catch species was composed of swimming crab (87%), Thomas's rapa whelk Rapana venosa (5%), arthritic neptune Neptunea cumingi (2%), Japanese swimming crab Charybdis japonica (2%), marbled sole Pleuronectes yokohamae (1%), pen shell Atrina pectinata, ocellate spot skate Okamejei kenojei, and so on. The carapace length (CL) of each swimming crab caught in the experimental nets was measured. Sponge crab (egg-bearing female crab) was appeared from the end of June to August in the coastal waters. The master selection curve was estimated by applying the extended Kitahara's method. The selection curve showed that the gill nets of larger mesh size allowed more crabs of small carapace size to escape. The optimum value (CL/m) was 0.645 and the CL/m was estimated to be 0.301, 0.354, 0.391, 0.422, and 0.450 when the retention probabilities were 0.1, 0.2, 0.3, 0.4 and 0.5, respectively.

To study the catching efficiency of biodegradable trammel net for swimming crab (Portunus trituberculatus), three types (biodegradable, monofilament and multifilament) of trammel nets were used in the field test, and the tests were carried out 16 times with two different mesh sizes (105mm and 160mm) in the Yeonpyeong fishing ground of Korea, 2009~2011. The catching efficiency of three type nets was analyzed by catch in number, catch in weight and average weight per individual of small and large size swimming crab by net types and mesh sizes. Statistical T-test was also carried out to verify the efficiency between the three types of nets. The results are as follows. The catch in number of swimming crab was 24,667 and formed about 81.0% of total catch. Of all swimming crab catch, small swimming crabs with less than 64mm in carapace length which is a prohibited landing size by law formed 48.1%, larger swimming crabs with more than 64mm in carapace length which is a landing size formed 51.9%. In 105mm mesh size trammel net test, the catch share in number of small size swimming crab by biodegradable trammel net was 47.5% in comparison with multifilament trammel net and 74.2% in comparison with monofilament trammel net, so biodegradable trammel net has more protective effects on small size swimming crab than other types of trammel nets. The protective effects for small size swimming crab by biodegradable trammel net was 25.8~52.5% in comparison with other types of trammel nets. The catch share in weight of large size swimming crab by biodegradable trammel net was 98.3% in comparison with multifilament trammel net and 92.3% in comparison with monofilament trammel net, so biodegradable trammel net has a similar catch efficiency to multifilament trammel net. The results of 160mm mesh size trammel net test have shown similar results of 105mm mesh size trammel net test. This study shows that biodegradable trammel net is a more useful fishing gear than multifilament and monofilament trammel net because biodegradable trammel net has lower catch rate than other types of nets in small size swimming crab and similar catch rate than multifilament trammel net which is a well used net by fishermen.

영광 주변해역에서 꽃게의 갑폭조성, 상대성장, 성비와 외포란량을 조사하였다. 조사해역에서 출현한 꽃게는 총 658개체(수컷:305, 암컷:353)로 나타났다. 조사기간 동안 수온과 염분의 변화 양상은 수온은 5.9-27.3℃의 범위를 나타내었으며, 염분 농도의 경우 28.1-31.6 psu의 범위를 각각 나타내었다. 두흉갑폭(CW)과 갑장(CL)의 관계와 두흉갑폭(CW)과 전중량(TW)의 관계식의 경우 암컷의 경우 CL = 1.3073 CW + 25.684(R2=0.791), TW=0.0061CW2.4231(R2=0.801)로 나타났고, 수컷의 경우 CL = 1.3652 CW + 22.495(R2= 0.741), TW=0.0068CW2.400(R2=0.749)로 나타났다. 꽃게의 성비의 경우 수컷과 암컷의 출현비율이 평균 0.86 : 1로 나타났다. 외포란량의 경우 암컷의 크기가 증가함에 따라 포란량은 증가함을 알 수 있었다.

Swimming crabs, Portunus trituberculatus(Miers) are commercially important off the coasts of Korea, Japan and China. Harvest of swimming crabs has been fluctuated along their distribution ranges. Fluctuations in the interannual harvest of swimming crabs may be correlated with the survival rate during the larval period. The survival rates, intermolt periods, and growth of larval swimming crabs were investigated in the laboratory. Larval swimming crabs are released and undergo development from April to August off the western coast of Korea in the Yellow Sea. Sea surface temperatures off the western coast of Korea during the larval season were used for the laboratory experiments, and ranged from 22 to 26℃. Larvae were individually cultured at four different temperatures, 22℃, 24℃, 26℃, and 28℃. Zoea molted to megalopa at all temperatures and developed to the first crab stage at 24℃, 26℃, and 28℃. Survival rates from zoea I to the first crab stage increased with increasing temperatures. Intermolt period and the growth rate of the mean carapace length were inversely correlated with temperature. Our research helps understand the changes in survival rate and growth of larval swimming crabs resulting from changing oceanic temperatures. Further, our study suggests that the fluctuations in fishery harvest of swimming crabs off the coast of Korea may be related to changes in larval survival affected by changing ocean conditions.

본 연구는 갯벌을 이용하는 민꽃게(Charybdis japonica) 개체군의 섭식생태를 파악하기 위하여 수행되었다. 표본 채집을 위한 현장조사는 1999년 11월부터 2001년 1월까지 매월별로 변형된 오타트롤(modified otter trawl)을 이용하여 실시하였다. 섭식특성 파악을 위한 위내용물 분석은 총 273개체를 대상으로 하였다. 위내용물 분석 결과, 본 종의 주된 먹이생물은 절지동물문 갑각류의 게류 (Brachyura), 만각류 (Cir

In order to develop the substitutive materials for natural baits of swimming crab pots, the attracting effects of swimming crab such as the preference of baits which were made of the by-products of marine and stock raising through the water tank experiments and fishing experiments. On the investigation of mean entrapped catch number to the pot by the baits after putting the 4 kinds of baits, mackerel(M), mackerel with grinded mackerel s internals(MMI), mackerel with tuna s internalsMMI) and makerel with grinded krill(MK) each in one pot by turns, MMI and MK were entrapped mean 3.9(13.0%) and they were a little more comparing to M, and MTI is least with mean 2.1(7.0%)(F=12.913, P 〈 0.05). Otherwise, on the preference investigation of swimming crabs by the baits after putting the 4 kinds of baits in the 4 pots each, M was entrapped mean 3.0(10%), but MMI, MTI and MK were mean 1.2(4.0%), 1.0(3.3%) and 1.5(5.0%) each and they were only 30-50% of M(F=13.398, P 〈 0.05). On the preference investigation of swimming crabs by the 5 kinds of baits, mackerel(M), and krill(K), manila clam(MC), pig s fat(PF) and chicken s head(CH) which were used in substitutive baits, M was entrapped mean 3.2(10.7%), but K was about 50% of catch of M with mean 1.6(5.3%), and MC, PF and CH were very few with mean 0.1-0.2(0.3-0.7%)(F=89.186, P 〈 0.05). On the preference investigation of swimming crabs by the pots which were put each the 3 kinds of baits, original krill(K), grinded krill with gluten and soybean oil cake(KGGS) and grinded krill with gluten, soybean oil cake and glycine(KGGSGL) in the blue fluorescent hexahedral plastic bait cages(BF), and which were put the mackerel(M) in the non-fluorescent hexahedral red plastic bait cage(RFN), it was entrapped mean 3.0(10.0%) in the pot which was put the mackerel in the RFN, and the same level in the pots which were put the K and KGGSGL in the BF, but it was mean 2.0(6.7%) in the pots which was put the KGGS in BF and it was decreased by 30% of catch comparing to RFN(F=3.750, P 〈 0.05). On the preference investigation of swimming crab by the pots which was put grinded tuna with gluten, soybean oil cake and glycine(TIGSGL) in the blue fluorescent hexahedral plastic bait cage(BF), and which was put mackerel(M) in the nonfluorescent hexahedral red plastic bait cage(RFN), it was entrapped mean 3.3(11.0%) in the pot which was put mackerel in RFN, and mean 2.7(9.0%) in the pot which was put TIGSGL in BF and it was about 15% less comparing to use bait M(t=1.387, P 〈 0.05). As a results of fishing experiments, a plan for enhancing catching efficiency of TIGSGL will be required because catching efficiency of TIGSGL, alternative bait, was half of fish catching efficiency of natural bait using mackerel. Fishing experiments were conducted 3 times using reinforced substitutive artificial bait that is reinforced attractive effect of TIGSGL and composed of tuna intestine, grinded mackerel, gluten, soybean cake, glycine and alanine(TIMGGSGLA). Catching efficiency of TIMGGSGLA was about 80% of that of natural bait made of mackerel.

In order to develop the substitutive materials for natural baits of swimming crab pots, the fluorescent characteristics of the baits were analyzed, and the preference of fluorescent dyes were investigated by the mean entrapped catch number to the pots through the water tank experiments and fishing experiments. On the investigation of fluorescent characteristics by the 5 kinds of baits, mackerel, krill, manila clam, pig's fat and chicken's head which were used in substitutive baits for test in the UV long wave(365nm) area, it showed clear blue fluorescence in the skin of mackerel, shell of krill, manila clam and bill of chicken's head, and green fluorescence in the mackerel s muscle and internals, and yellow fluorescence in the pig's fat and chicken's head. On the investigation of fluorescent characteristics by the bait cages in the UV short wave(254nm) and long wave(365nm) area, it showed each green, red and blue fluorescence in the cylinderical or hexahedral red plastic bait cages which were painted each green, red and blue fluorescence dyes, but it showed yellowish green flourescence in the cylinderical or hexahedral red plastic bait cage which was painted yellow fluorescent dye. On the preference investigation of the fluorescent dyes of swimming crabs by the 5 kinds of the bait cages which were put the mackerel in the non-fluorescent red plastic cage(RFN), red, yellow, green and blue fluorescent plastic cages(RF, YF, GF, BF) each, nonfluorescent red plastic cage(RFN) was entrapped mean 2.0(6.7%), but blue fluorescent plastic cage(BF) was mean 5.0(16.7%) and it was more 2.5 times comparing to RFN, and red fluorescent cage(RF) was same level and green fluorescent cage(GF) was 50% of catch number comparing to RFN, and yellow fluorescent cage(YF) was entrapped nothing(F 46.324, P 〈 0.05). On the investigation of the entrapped catch number to the pots which were put the mackerel in the blue fluorescent hexahedral plastic cage(HP) and blue fluorescent silicon mackerel model cage(SM), HP was mean 3.4(11.3%) and it was a little more comparing to SM which was entrapped mean 3.2(10.7%)(t 0.775, P 〉 0.05). Fishing experiments on the preference investigation of swimming crabs by the pots which were put in the non-fluorescent red plastic cage(RFN) and blue fluorescent plastic cage(BF) were conducted 3 times. Mean catching number and weight of RFN were 71.7 ind.(18.3%) and 16.9kg(64.3%), and those of BF were 93.0 ind.(23.1%) and 19.8kg(64.5%), respectively.

In order to evaluate the biodegradability of bait used in the pot for swimming crab, water tank experiments were conducted. Mackerel is the most commonly used natural form of bait to catch the swimming crabs, and therefore was used in this experiment for the biodegradability according to the manufacturing process of the bait. From the biodegradability test on chemical oxygen demand(COD), total nitrogen(T - N), total phosphorus(T - P), ammoniac nitrogen, nitrite nitrogen and nitrate nitrogen per unit weight of the bait based on the production rate and the accumulated amount of pollutants, it was concluded that the smaller the size of the mackerel pieces, the higher the production rate and accumulated amount of organic matter and nutrients which was unfavorable to water pollution. The amount of pollutants released from the intestine of the tuna was similar with that from the whole mackerel. For the operation period of 111 days, the accumulated concentrations of tested pollutants from the tuna which were 67.3 mgCOD/g d, 86.4 mgT N/g d, 3.1 mgT - P/g d, were almost half comparing with those from the mackerel which were 65.7 - 94.4 mgCOD/g d, 83.8 - 109.4 mgT - N/g d, 3.1 - 5.2 mgT - P/g d. The amount of pollutants released from the intestine of the tuna was slightly less than that from the mackerel that was cut into 8 pieces. but more than that from the mackerel which was not cut into pieces. Therefore, it can be concluded that the key factor in determining water pollution potential is not the kind of bait, but the processing or preparation method used.

In order to develop the artificial bait of trap for swimming crabs, the attracting effect was analyzed in the duration time of attracting effect, preference and shape recognition to the bait in the water tank and field experiments. According to the result which investigated the mean entrapped number of swimming crabs into the trap for each bait(mackerel, sardine and anchovy) in water tank experiments, the mean entrapped number was found to be higher in the trap with mackerel(6.9 ind.; 23.0%) than in other traps with sardine(5.2 ind.; 17.3%) and anchovy(3.4 ind.; 11.3%). In addition, the mean entrapped ratios of swimming crabs into the traps for 3 kinds of baits were estimated to be over 80% all in 5 hours after soaked in the water tank. For preference of swimming crabs to 3 kinds of baits, the trap with mackerel(6.9 ind.; 23.0%) was evaluated to be higher than the traps with sardine(2.8 ind.; 9.3%) and anchovy(0.2 ind.; 0.7%). In the field experiments, the mean entrapped number of swimming crabs into the trap with mackerel and sardine were found to be similar as 120 and 118 ind., but the trap with anchovy was only 85 ind.. According to the result which investigated the shape regnition of swimming crabs to the bait used mackerel, divided into 8 pieces, 16 pieces and grinded mass in water tank experiments, the mean entrapped number of swimming crabs into the traps that includes the mackerel divided into several pieces was significantly decreased. Moreover, by the presence of skin of mackerel for bait, there was a great difference in the entrapped number of swimming crabs. Therefore, it was thought that the swimming crab was attracted to the bait and entrapped into the trap would indicate that not only the chemical stimuli such as the attactant, but also the visual stimuli such as the shape recognition to the bait might be closely related.