이 연구의 목적은 척추 자기공명영상 검사 시 기존의 포화 펄스와 함께 호흡 유도하(respiratory triggering, RTr) 기법을 적용하는 것이 영상화질 개선에 있어 효과적인지를 평가해 보기 위함이다. 이에 본 연구는 2024년 2월부터 2024년 10월까지 척추 MRI 검사를 받은 환자를 대상으로 분석하였다. 사용된 장비는 GE 사의 3.0T SIGNA Premier을 사용하였고, 축상 T2강조영상에서 동일한 부위를 RTr 기법을 적용하지 않은 영상과 RTr 기법을 적용한 영상 두 개를 획득해 평가하였다. 정성평가는 2명의 평가자의 평가점수를 Wilcoxon test로 비교하였다. 정성평가 결과, 경추와 요추에서는 RTr 기법 적용 시 영상화질 개선에 있어서 유의미한 차이를 보이지는 않았지만 흉추에서는 RTr 기법 적용 시 영상화질 개선에 있어서 유의미한 차이를 보였다. 결론적으로 경추와 요추보다는 포화펄스 사용에 있어 제한이 있고 호흡에 영향을 많이 받는 흉추 MRI 검사 시 RTr 기법을 적용하는 것이 더 효과적이다. 다만 RTr 기법 적용 시 검사 시간이 늘어나는 단점이 있어서 환자의 상태에 맞춰 검사하는 것이 요구되는 바이다.

Habitat environment and food sources of fish, benthic macroinvertebrates, and brown trout (Salmo trutta) downstream of Soyang River Dam were analyzed. Water temperature at the site where brown trout was identified ranged from approximately 12.4 to 13.4°C, confirming that this environment could provide an optimal water temperature for the growth of brown trout. Most of the riverbed structures at this site had a high proportion of cobble and pebble substrates. Brown trout constituted less than 5% of the total fish population, more abundant in the upstream. The total lengthweight relationship of brown trout showed a parameter b value of 3.234, with the condition factor (K) increasing with length. Dominant benthic macroinvertebrates were Limnodrilus gotoi and Chironomidae spp. (non-red type). Stomach content analysis indicated that brown trout primarily consumed aquatic insects (R.A., 73.8%), non-insects (R.A., 23.3%), ground insects (R.A., 2.7%), and fish (R.A., 0.2%, TL: 246 mm). Correlation analysis revealed a positive relationship between total length and species preferring flowing water (p<0.05) and a negative relationship of total length with species favoring low-flow, sandy habitats (p<0.05). Larger brown trout showed active feeding behavior and resilience to flow speed and riverbed structure. The primary food source for the largest brown trout (TL: 246 mm) was Hypomesus nipponensis. Future analyses should include brown trout with a total length of 30 cm or more. Given that samples were limited, comprehensive population management will require ongoing research.

이 연구의 목적은 척추 자기공명영상 검사 시, 검사시간을 단축할 수 있는 기법인 SENSE와 CS-SENSE를 경추, 흉추, 요추 검사 시 어떤 기법을 적용하는 것이 효과적인지 알아보기 위함이다. 본 연구는 2022년 10월부터 2023년 3월까지 척추질환 및 의심 환자 102명을 대상으로 분석하였다. 사용된 장비는 필립스 사의 3.0T Ingenia Elition X와 20채널 두경부 코일과 44 채널 척추 코일을 사용하였다. 본 연구는 축상 T2강조 고속스핀에코를 SENSE와 CS-SENSE factor 1.5, 2, 3으로 각각 영상을 획득하고, 정량평가는 신호대잡음비와 대조대잡음비를 각 factor 별로 대응 표본 t-test를 진행하였고, 정성평가는 2명의 평가자의 평가점수를 Kruskal-wallis test로 비교하였다. 정량평가 결과, SENSE보다 CS-SENSE가 신호대잡음비와 대조대잡음비가 높게 나왔다. 정성평가 결과, 경추, 요 추는 CS-SENSE 사용 시 평가점수가 높게 나왔고, 흉추는 SENSE 사용 시 평가점수가 높게 나왔다. 결론적으로 척추 MRI 검사 시, 경추, 요추는 CS-SENSE를, 흉추는 SENSE를 사용하는 것이 효과적이다.

본 연구는 최근 7년간 Google scholar, ProQuest에서 Adolescent Idiopathic Scoliosis(AIS), Cobb’s angle, Surgical, Conservative, In tervention을 핵심 키워드로 활용하여 청소년 특발성 척추측만증의 수술 적 중재 방법과 보존적 중재 방법이 Cobb’s angle에 미치는 영향을 조 사하였다. 중재 전ㆍ후 Cobb’s angle 차이를 JAMOVI를 이용하여 메타 회귀분석을 실시하였으며 청소년 특발성 척추측만증 환자에게 Cobb’s an gle 긍정적 변화에 효과적인 중재 방법은 수술적 중재 방법에는 Selectiv e thoracic fusion, Posterior spinal fusion, Anterior spinal fusion 이 있었으며, 보존적 중재 방법에는 Lumbar cheneau brace, Xinmiao treatment system(XTS), Cheneau brace+Schroth best practice(S BP), Core stabilization exercises가 있음을 확인하였다. 이러한 연구 결과를 통하여 청소년 특발성 척추측만증 연구 분야의 방향성 제공에 도 움을 주고 향후 어떠한 중재 방법이 효과적일지 판단할 수 있는 지표개발 을 요구하는 바이다.

Most wetlands worldwide have suffered from extensive human exploitation. Unfortunately they have been less explored compared to river and lake ecosystems despite their ecological importance and economic values. This is the same case in Korea. This study was aimed to estimate the assemblage attributes and distribution characteristics of benthic macroinvertebrates for fifty wetlands distributed throughout subtropical Jeju Island in 2021. A total of 133 taxa were identified during survey periods belonging to 53 families, 19 orders, 5 classes and 3 phyla. Taxa richness ranged from 4 to 31 taxa per wetland with an average of 17.5 taxa. Taxa richness and abundance of predatory insect groups such as Odonata, Hemiptera and Coleoptera respectively accounted for 67.7% and 68.2% of the total. Among them Coleoptera were the most diverse and abundant. Taxa richness and abundance did not significantly differ from each wetland type classified in accordance with the National Wetland Classification System. There were three endangered species (Clithon retropictum, Lethocerus deyrolli and Cybister (Cybister) chinensis) and several restrictively distributed species only in Jeju Island. Cluster analysis based on the similarity in the benthic macroinvertebrate composition largely classified 50 wetlands into two major clusters: small wetlands located in lowland areas and medium-sized wetlands in middle mountainous regions. All cluster groups displayed significant differences in wetland area, long axis, percentage of fine particles and macrophyte composition ratio. Indicator Species Analysis selected 19 important indicators with the highest indicator value of Ceriagrion melanurum at 63%, followed by Noterus japonicus (59%) and Polypylis hemisphaerula (58%). Our results are expected to provide fundamental information on the biodiversity and habitat environments for benthic macroinvertebrates in wetland ecosystems, consequently helping to establish conservation and restoration plans for small wetlands relatively vulnerable to human disturbance.

출혈성 담낭염은 담낭염의 형태 중 드문 것으로, 특히 그동안 출혈성 무결석 담낭염에 대한 보고는 제한적으로 이루어져 왔다. 본 증례보고에서는 척추추간판염으로 입원 중인 환자에게서 간기능 검사의 이상 소견이 발생하여 시행한 현장초음파상에서 보인 이상 소견을 기반으로 출혈성 담낭염을 의심하였다. 환자는 문헌상 초음파상에서 출혈성 담낭염의 특징으로 제시된 담낭벽의 불규칙한 비후, 후방 음영이나 층적을 동반하지 않는 내강 안 에코의 소견 등을 만족하였으며, 이어서 촬영한 CT에서도 조영제 누출의 소견이 보여 혈역학적으로 안정된 상태에서 조기에 경피적 배액술과 후속 담낭절제술을 시행하였다. 절제된 검체에서는 혈괴와 함께 화농성 담낭염 소견이 확인되었다. 기존에 항혈소판제, 항응고제 등의 약제 복용이나 알려진 출혈 경향이 없었던 화농성 척추추간판염 환자에게서 출혈성 무결석 담낭염을 현장초음파로 합병증 발생 전에 진단하여 치료한 증례로서 보고하는 바이다.

본 연구는 최근 10년간 국내 청소년 특발성 척추측만증 보존적 중재법이 Cobb's angle의 변화에 어떠한 영향을 미치는지 알아보기 위하여 RISS(학술연구정 보서비스)에서 청소년 척추측만증, 특발성, 중재법을 핵심키워드로 검색하여 메타 회귀분석을 실시하였다. KCI등재후보지 이상 학술지만 선별하였으며, 참가자의 연 령 및 중재방법 등을 고려하여 총 11개의 논문이 선정되었다. 총 23개의 보존적 중재법은 크게 능동적 운동치료, 수동적 치료, 복합치료, 보조기로 나뉘었으며 신 뢰도95%에 해당하는 중재법은 능동적 운동치료 5가지와 보조기 1가지였다. 이는 능동적 운동치료의 형태의 보존적 중재법이 청소년 특발성 척추측만증 Cobb's angle변화에 가장 긍정적인 영향을 미치는 것으로 조사되었다.

Paddy fields provide important habitats for biodiversity conservation within the agricultural ecosystem. Their importance is gradually increasing as their ecological value is better understood. Benthic macroinvertebrates dominate paddy fields. They play an essential role in maintaining the biodiversity of paddy ecosystems. This study aimed to analyze characteristics of benthic macroinvertebrate communities and main environmental factors affecting the distribution of golden apple snails (Pomacea canaliculata). Results showed that the diversity index (H ′) of the benthic macroinvertebrate community was the highest at the Sangju site (St. 12) but the lowest at the Sancheong site (St. 18). Total Dissolved Solids (TDS), salinity, and Electrical Conductivity (EC) values were the highest in Gimhae and Yeongam based on Canonical Correspondence Analysis (CCA). Numbers of P. canaliculata (m-2) were relatively low in Gunsan and Iksan where water temperatures were high. Therefore, changes in geographical characteristics and environmental factors might affect the distribution of P. canaliculata and characteristics of benthic macroinvertebrate communities. Results of this study can be used as primary data for biodiversity conservation and ecosystem service evaluation in agroecosystems.

In stream ecosystem assessment, RIVPACS, which makes a simple but clear evaluation based on macroinvertebrate community, is widely used. In this study, a preliminary study was conducted to develop a RIVPACS-type model suitable for Korean streams nationwide. Reference streams were classified into two types (upstream and downstream), and a prediction model for macroinvertebrates was developed based on each family. A model for upstream was divided into 7 (train): 3 (test), and that for downstream was made using a leave-one-out method. Variables for the models were selected by non-metric multidimensional scaling, and seven variables were chosen, including elevation, slope, annual average temperature, stream width, forest ratio in land use, riffle ratio in hydrological characteristics, and boulder ratio in substrate composition. Stream order classified 3,224 sites as upstream and downstream, and community compositions of sites were predicted. The prediction was conducted for 30 macroinvertebrate families. Expected (E) and observed fauna (O) were compared using an ASPT biotic index, which is computed by dividing the BMWPK score into the number of families in a community. EQR values (i.e. O/E) for ASPT were used to assess stream condition. Lastly, we compared EQR to BMI, an index that is commonly used in the assessment. In the results, the average observed ASPT was 4.82 (±2.04 SD) and the expected one was 6.30 (±0.79 SD), and the expected ASPT was higher than the observed one. In the comparison between EQR and BMI index, EQR generally showed a higher value than the BMI index.

On April 29, 2021 (1st), June 2 (2nd), and August 17 (3rd), we surveyed benthic macroinvertebrates fauna at Muljangori-oreum wetland in Bonggae-dong, Jeju Island, Korea. Muljangori-oreum wetland was divided into four areas. The survey was conducted in three accessible areas (areas 1-3). As a result of habitat environment analysis, the average monthly temperature from 2017 to 2021 was the highest in July and August and the lowest in December and February. This pattern was repeated. As a result of analyzing changes in vegetation and water surface area through satellite images, normalized difference vegetation index (NDVI) increased from February to July and decreased after July. Normalized difference water index (NDWI) was analyzed to show an inverse relationship. A total of 21 species from 13 families were identified in the qualitative survey and a total of 412 individuals of 24 species from 15 families were identified in the quantitative survey. A total of 26 species from 17 families, 8 orders, 3 classes, and 2 phyla of benthic macroinvertebrates were identified. The dominant species was Chronomidae spp. with 132 individuals (32.04%). Noterus japonicus was a subdominant species with 71 individuals (17.23%). As a result of comparative analysis of species identified in this study and the literature, it was confirmed that species diversity was high for Coleoptera and Odonata. Main functional feeding groups (FFGs) were found to be predators. Habitat orientation groups (HOGs) were found to be swimmers. In OHC (Odonata, Hemiptera, and Coleoptera) group, 17 species (73.91%) in 2021, 23 species (79.31%) in 2016, 26 species (86.67%) in 2018, and 19 species (79.17%) in 2019 were identified. Cybister japonicus, an endangered species II, was confirmed to inhabit Muljangori-oreum wetland in the literature. Ten individuals (2.43%) were also confirmed to inhabit Muljangori-oreum wetland in 2021. Therefore, continuous management and habitat protection are required to maintain the habitat environment of C. japonicus in Muljangori-oreum wetland.

서남해안 대형저서무척추동물의 출현종 조성을 알아보기 위해 완도와 조도 18개 정점의 암반조간대에서 2014년부터 2021년 까지 6~9월에 7회 조사하였다. 완도와 조도 암반조간대에서 출현한 대형저서무척추동물은 220종, 645개체/㎡이었다. 종수는 완도 165종, 조도 167종으로 유사하였다. 평균밀도는 완도 726개체/㎡, 조도 564개체/㎡로 완도가 다소 높았다. 종수는 정점 15에서 116종으로 가장 많았고, 밀도는 정점 7에서 1,664개체/㎡로 높게 나타났다. 우점종은 좁쌀무늬총알고둥이 175개체/㎡(27.1%)로 가장 우점하였고, 총알고 둥 97개체/㎡(15.1%), 갈고둥은 41개체/㎡(6.3%)순으로 출현하였다. 기후변화지표종인 검은큰따개비가 15개 정점에서 출현하였고, 고유종 인 털채찍해조숨이옆새우도 일부 정점에서 출현하였다. 국가적색목록(Red List) 중에 절멸위험(Extinction risk)의 가장 낮은 수준인 준위 협의 모난어깨긴뿔고둥이 조도에서 출현하였다. 완도와 조도의 대형저서무척추동물은 우리나라 암반조간대 해안에서 나타나는 일반적 인 특징을 보였다. 암반조간대 해안은 조위에 따른 노출 시간과 암반의 기질에 따라 수직적인 분포 경향을 보였으며, 완도와 조도는 좁쌀무늬총알고둥, 총알고둥, 조무래기따개비, 대수리 등 우점하여 서식지 분포 양상이 유사하였다.

Suspended solids play an important role in the growth and survival of aquatic organisms. The marine zooplankton species tested in this study were Tigriopus west (Copepoda) and Haustorioides koreanus (Amphipoda) sampled from the intertidal zone, including Artemia nauplii (Branchiopoda) hatched from cysts. The study design included six concentrations (0, 50, 100, 250, 500, and 1,000 mg L-1) of the suspended test particles assayed in triplicate. Experimental cultures in 500 mL-round polycarbonate bottles were subsampled after 96 h to count dead zooplankton. The culture bottles were agitated at 4 RPM on a rotating wheel at 23°C and 30 PSU. The survival rates of Artemia nauplii and T. west were not affected by suspended solid concentrations higher than 50.0 mg L-1, whereas the survival rate of H. koreanus decreased with increasing concentrations (p<0.05). In conclusion, H. koreanus and T. west, which were continuously exposed to suspended solid concentrations higher than 50.0 mg L-1, were affected by low-intensity ecological stress. However, in the case of H. koreanus, a concentration of 50.0 mg L-1 may be considered to be the limit of tolerance to suspended solids, suggesting that the number of individuals who eventually die will increase if continuously exposed.

The macroinvertebrate community in the Singal reservoir, Yedang and Juam lake was investigated three times from April 2021 to October 2021. Each lake was investigated by dividing it into inflow, middleflow, and outflow. Additionally, sampling was conducted again by dividing it into the edge and center parts at each inflow, middle-flow, and outflow. Eight families of benthic macroinvertebrates were collected except for chironomids in the sampling sites. Dominant macroinvertebrates were investigated as chironomids, and Tubificidae was sub-dominant organisms. The density of macroinvertebrate community was higher in the edge area than in the center bottom of the lakes. The density of chironomids was low when the water level was high but was high when the water level was low. In the edge area of the middle-flow in Singal reservoir, the density of chironomids was 1,208 ind. m-2 in April when the water level was high, but it increased to 1,401 ind. m-2 in July when the water level was low. Similarly, the density of chironomids at the outflow of Yedang lake was high (1,990 ind. m-2) in July when the water level was low. The density of chironomids also decreased along with the increasing water level at all edge areas of Juam lake. These results indicated that it will be necessary to consider the water level when studying macroinvertebrate communities in the lake.

Ecological occupation in irrigation ponds is a well-acknowledged fact that is essential for biodiversity conservation in agricultural ecosystems. However, there are few studies on the ecological functions and relationship between a paddy field and irrigation using macroinvertebrates in an environmentally friendly paddy field. The objective of this study is to identify the community and distribution characteristics of macroinvertebrates in an agricultural paddy field and irrigation pond ecosystems, and to provide basic data on the ecological function of an environmentally friendly paddy field. Macroinvertebrate sampling was conducted from May to September in an agricultural paddy field and irrigation pond in an environment-friendly paddy field in Boryeong city. We conducted a study to identify the distribution characteristics using macroinvertebrate species analysis, such as Functional Feeding Groups (FFGs), Habitat Oriented Groups (HOGs), rarefaction curve, and a two-way dendrogram. A total of 37 species of macroinvertebrates in 28 families, 13 orders were collected study during the period of the investigation. Dominant taxa of macroinvertebrates included Coleoptera, Hemiptera, and Odonata. In terms of FFGs, predators and gathering collectors accounted for approximately 70%, in relation to HOGs, and climbers and swimmers occupied more than 50% from both the paddy field and irrigation pond. With respect to the rarefaction curve, the irrigation pond (July) was high as E (S, 141)=18 species, while the paddy field (May) was comparatively low as E (S, 141)=9 species. In conclusion, our results revealed that macroinvertebrates, such as Notonecta triguttata, Peltodytes intermedius, Appasus major, Laccotrephes japonensis, Appasus japonicus, Sigara substriata, Enochrus simulans, and Sternolophus rufipes, were used as a habitat and spawning ground in both paddy field and irrigation pond. The irrigation pond appears to be a very important spawning ground for macroinvertebrates.

Koreoleptoxis nodifila (Martens, 1886) is an endangered species only living in the central and north streams of South Korea. However, there is a lack of information on physical habitat characteristics of K. nodifila. We aimed to determine preference ranges for water depth, current velocity, streambed substrate of K. nodifila. The weibull model was used to estimate the habitat suitability based on distribution of individual abundance by physical factors. Optimal depth preferences ranged from 0.53~17.17 cm, current preferences ranged from 48.40~81.03 cm s-1 and substrate (Φm) preferences ranged from - 4.36~ - 2.26. Median values of central tendency were determined as follows: water depth 16.73 cm, current velocity 65.23 cm s-1, substrate - 3.51. Mean values of central tendency were determined as follows: water depth 21.32 cm, current velocity 65.65 cm s-1, substrate - 3.63. Mode values of central tendency were determined as follows: water depth 5.17 cm, current velocity 64.77 cm s-1, substrate - 3.24. Based on the habitat suitability analysis, the microhabitat types of K. nodifila were determined as riffle and coarse-grained streambed.

본 연구는 2020년 5월부터 10월까지 4대강 사업의 하나로 준공된 8개의 낙동강 보 구간에 서식하는 저서성 대형무척 추동물의 생물다양성 변화와 군집분석을 수행하였으며, 2010년부터 2020년까지 약 10년간의 저서성 대형무척추동물 주요 분류군에 대한 변화상을 분석하였다. 조사결과 저서성 대형무척추동물은 총 3문 5강 18목 52과 83속 97종 128.1개 체/m2가 출현하였으며, 오염지표생물인 깔따구류(Chironomidae sp.)와 실지렁이류(Tubificidae spp.)가 전체적으로 우점하였다. 군집분석결과 우점도지수는 평균 0.62±0.20이고 다양도지수는 1.87±0.63로 낙동강 보 상류에서 가장 안정적으로 분석되었다. 낙동강 보 중앙부에서는 실지렁이가 우점하였으며, 칠곡보와 강정고령보에서 가장 불안한 군집구조가 나타났다. 군집안정성 분석결과 상대적 회복력과 저항력이 높은 특성군I이 높게 나타났으며, 저항력과 회복력이 낮은 특성군III이 소수 분포하는 것으로 나타나 현재 낙동강 보 구간의 군집은 매우 불안한 상태로 분석되었다. 섭식기능군에서는 잡아먹는무리가 비교적 높게 출현하였고, 썰어먹는무리, 모아먹는무리, 걸러먹는무리는 낮게 나타났 다. 서식기능군에서는 굴파는무리와 기어오르는무리가 우세하게 출현하여 대부분 용존산소가 낮은 정수생태계에서 출현하는 기능군들이 많이 분포하고 있는 것으로 나타났다. 주요 법적보호종으로는 상주보와 낙단보에서 멸종위기야생 생물 II급인 노란잔산잠자리(Macromia daimoji)가 분포하고 있는 것으로 확인되었다. 과거부터 10년간의 분석결과 종수와 개체수는 댐 공사 직후에는 증가하였지만, 2016년 이후부터 급감하면서 다시 안정화되고 있는 것으로 나타났다. 우점종으로는 깔따구류가 모든 지점에서 우점하는 것으로 나타났으며, 현재 약 50% 수준의 비율로 유지되고 있는 것으로 나타났다. EPT 그룹은 2011년을 기점으로 대폭 감소하였으며, 이중 강도래목과 날도래목은 회복하지 못하는 것으로 분석되었다. 오염지표생물인 실지렁이류는 2012년 공사 완료 후 증가 추세를 보였으며, 2015년과 2020년에 개체수가 폭발적으로 증가하였다. 멸종위기야생생물 I급으로 지정된 귀이빨대칭이(Cristaria plicata)는 2011년 합천창 녕보에서 출현 후 관찰되지 않았으며, 멸종위기야생생물 II급인 노란잔산잠자리는 2015년까지 7개의 구간에서 드물게 확인되었으나 현재 분포 서식처가 급격히 줄어든 것으로 확인되었다.

This study analyzed the relationship between the community structure of benthic macroinvertebrates and habitat changes in open estuaries among the sites included in the national estuary monitoring program. The estuary survey was conducted under the “Guidelines for Investigation and Evaluation of Biometric Networks” and classified by sea area, 80 places in the East Sea, 102 places in the South Sea, and 19 places in the West Sea were investigated. In a total of 201 open estuaries, benthic macroinvertebrates were identified with 4 phyla, 9 classes, 41 orders, 139 families, 269 species and 196 species in the East Sea, 182 species in the South Sea, and 90 species in the West Sea. The highest population densities were Insecta in the East Sea, the Malacostraca in the South Sea, and the Annelida in the West Sea. Through SIMPER analysis, species contributing to the similarity of benthic macroinvertebrates communities in each sea area were identified. Some species greatly influenced the similarity of clusters. The benthic community in the East Sea was affected by the salinity, so the contribution rate of freshwater species was high. On the other hand, the benthic communities of the South and West Seas showed species compositions are influenced by the substrate composition. As results, the benthic macroinvertebrate community in Korean estuaries was impacted by salinity and substrate simultaneously, and the close relationship with geographical distance was not observed. The result of this study is expected to be used to respond to environmental changes by identifying and predicting changes in the diversity and distribution of benthic macroinvertebrates in Korea estuaries.

In 2020, the diversity of benthic macroinvertebrate communities was investigated in the Sohan stream, an ecological and landscape conservation area, and the results were compared with the previous research conducted in 2011. In total, 42 species (two phyla, three classes, and seven orders) were found in the Sohan stream. Species richness and abundance sharply decreased at all sampling sites because of Typhoons Haishen and Maysak in 2020, which had a direct impact on the stream. In the functional feeding group, the ratio of collector-gatherers was the highest at all sampling sites. However, during the autumn season, the shredder ratio increased from 13.4% to 42.4% in the uppermost stream site. Compared with the diversity of benthic macroinvertebrates surveyed in 2011, a total of 53 species (two phyla, three classes, and eight orders) were found. The percentage of species richness and abundance of Ephemeroptera, Plecoptera, and Trichoptera was more than 50% both in 2011 and 2020. Only the richness of Ephemeroptera was significantly different between them (2011: 2.9 and 2020: 6.7). In this study, the abrupt changes of species richness and abundance in benthic macroinvertebrate were not observed before and after the designation of an ecological and landscape conservation area. However, it is necessary to monitor benthic macroinvertebrates in order to confirm that biodiversity is continuously maintained long after the designation of the ecological and landscape conservation area.