The present study was conducted to develop persimmon peel, a by-product of dried persimmon manufacturing, as a feed additive via lactic acid bacteria fermentation. Pediococcus pentosaceus, Lactobacillus plantarum, and three strains of Leuconostoc mesenteroides were used as a starter culture in the solid state fermentation of persimmon peel, and antioxidant activity and total polyphenol content were assessed. Leuconostoc mesenteroides KCTC 3100 showed high antioxidant activity (p<0.05), whereas Pediococcus pentosaceus showed high total polyphenol content (p<0.05). These two strains were thus selected as starter culture strains. Glucose, sucrose and molasses were used as variables for optimization and a total 15 experimental runs were produced according to Box-Behnken design. Regarding significant effects of variables, molasses showed linear and square effects on antioxidant activity of persimmon peel fermentation (p<0.05). In conclusion, optimum concentrations of glucose, sucrose, and molasses were determined to be 4.2, 3.9 and 5.3 g/L, respectively, using a response surface model. Antioxidant activity was also improved 2.5 fold after optimization.

In order to understand food sources-metabolism for the pacific oyster (Crassostrea gigas), the stable isotope ratios of carbon (δ13C) and nitrogen (δ15N) of its gut, gill, and muscle as well as potential food sources (particulate o

Carbon materials of various morphologies were synthesized by pyrolysis of Soap-nut seeds (Sapindus mukorossi), Jack Fruit seeds (Artocarpus heterophyllus), Date-seeds (Phoenix dactylifera), Neem seeds (Azadirachta indica), Tea leaves (Ehretia microphylla), Bamboo stem (Bambusa bambus) and Coconut fiber (Cocos nucifera), without using any catalyst. Carbon materials thus formed were characterized by SEM XRD and Raman. Carbon thus synthesized varied in size (in μm) but all showed highly porous morphology. These carbon materials were utilized as the anode in Lithium secondary battery. Amongst the various precursors, carbon fibers obtained from Soap-nut seeds (Sapindus mukorossi) and Bamboo stem (Bambusa bambus), even after 100th cycles, showed the highest capacity of 130.29 mAh/g and 92.74 mAh/g respectively. Morphology, surface areas and porosity of carbon materials obtained from these precursors were analyzed to provide interpretation for their capacity to intercalate lithium. From the Raman studies it is concluded that graphitic nature of carbon materials assist in the intercalation of lithium. Size of cavity (or pore size of channels type structure) present in carbon materials were found to facilitate the intercalation of lithium.

서울시 지하수중 중금속 등으로 오염되어 음용 이외의 생활용수로 사용하고 있는 8개 정점과 음용수로 사 용하고 있는 2개 정점을 대상으로, 세균 군집 대사 다양성의 변화를 유일 탄소원 이용능의 변화 양상으로 분석하였다. Biolog GN plate에서 발색 반응이 안정화된 배양 시간은 155시간 이후로 나타났다. 음용수로 사용하는 정점이 생활용수로 사용하는 정점의 발색 반응값 보다 높게 나타났다. 지하수 세균 군집은 polymer, carbohydrate, ester 및 amino acid를 공통적으로 많이 이용하였으나, brominated chemicals과 phosphorylated chemicals를 이용하는 세균 군집은 적게 나타났다. 다변량 통계학적 분석을 통한 세균 군집의 대사 다양성 분석 결과, 모두 5개 그룹인 음용수 정점, 휘발성 오염 정점, 크롬 오염 정점, 질산성 질소 오염 정점과 비소 오염 정점으로 구분이 가능하였다. 오염물질에 따른 세균 군집의 탄소원 이용능의 차이로 볼 때, 오염원에 따라 세균 군집의 대사 다양성이 변화하고 있음을 확인하였다.

Production of Bacterial Cellulose (BC) by Gluconacetobacter sp. A5 was studied in shaken culture using different cost-effective carbon sources and its structural and mechanical properties were evaluated. Glycerol showed the highest level (7.26 g/l) of BC production, which was about three times higher than the yield in glucose medium. BC production depended not only on the decrease in pH, but also on the ability of Gluconacetobacter sp. A5 to synthesize glucose from different carbon sources and then polymerize it into BC. All BC produced from different carbon sources exhibited a three-dimensional reticulated structure consisting of ultrafine cellulose fibriles. Carbon sources did not significantly change the microfibrile structure of the resulting BC. BC produced from glucose medium had the lowest water-holding capacity, while BC from molasses medium had the highest. XRD data revealed that all BC were cellulose type І, the same as typical native cellulose. The crystalline strength of BC produced in glucose medium was the highest, and that in molasses medium was the lowest. Our results suggest that glycerol could be a potential low-cost substrate for BC production, leading to the reduction in the production cost, and also to produce BC with different mechanical properties by selecting appropriate carbon source.

Background : Radish sprouts or young seedlings are important nutritional vegetables in Asian countries. In the present study, we investigated plant growth and levels of glucosinolate accumulation in radish sprouts in response to treatments with different carbon sources. Plant growth and accumulation of glucosinolates were inversely correlated in treatments with different carbon sources. Methods and Results : All the carbon sources used in this study inhibited the growth of shoot and root in radish sprouts and facilitated significantly the accumulation of glucosinolates. Seven different glucosinolate compounds were detected in radish sprouts treated with different carbon sources. The total as well as individual amounts of all the identified glucosinolates increased after treatments with different carbon sources, except for 4-methoxyglucobrassicin and glucoiberin. In particular, the supplementation of sucrose, galactose, and glucose resulted in highest glucosinolate accumulation in radish sprouts. Radish sprouts treated with sucrose showed the highest levels of total glucosinolates, which was 1.22-fold higher than that of untreated sprouts. Furthermore, sucrose treatment resulted in higher production of gluconapoleiferin, glucoerucin, glucoraphasatin, and glucobrassicin compared with that in untreated sprouts, and the treatment of galactose and glucose similarly enhanced glucosinolate production when compared with untreated sprouts. Conclusion : seven glucosinolates were identified by HPLC and great differences in glucosinolates levels were observed in radish sprouts in response to different carbon sources. The glucosinolate production was positively affected by most of the stimuli used in this study. The results presented herein provide information about optimal cultivation conditions, particularly the suitable carbon sources, that will enhance glucosinolate production in radish sprouts.

This study was conducted to investigate the effects of inoculum and carbon sources on anaerobic digestion characteristics. The treatments were combinations of inoculum (digestate of cattle manure and rumen fluid) with carbon sources (starch, cellulose, and xylan). Anaerobic digestion was performed in triplicate at 37°C for 18 days at 100 rpm. Sampling was performed at 0, 1, 2, 3, 4, 5, 7, 9, 12, 15, and 18 days to measure pH, ammonia-N, volatile solids reduction, the cumulative methane content, and the cumulative methane production. There was a significant difference in methane content depending on the carbon source and there was a significant difference in pH, ammonia-N, methane production, and methane content depending on the inoculum (P < 0.05). The results of methane production were higher in the digestate of cattle manure treatment than in the rumen fluid treatment (P < 0.05). In this study, different digestive patterns depending on the type of carbon source could be used as basic research data to set the hydraulic residence time of anaerobic digestion facilities. In addition, the use of ruminal fluid as an inoculum may help accelerate the hydrolysis and acid production steps.

This study was conducted to investigate the effects of different carbon sources on the anaerobic fermentation characteristics in the startup phase using the biochemical methane potential test. The treatments for this experiment were combinations of carbon sources (starch, cellulose, and xylan). Anaerobic fermentation was done at 37oC for 18 days with agitation and pH, ammonia nitrogen, volatile solids reduction, gas production, methane content, and methane production were investigated at 0, 1, 2, 3, 4, 5, 7, 9, 12, 15, and 18 days after incubation in triplicate. In the experiment, the pH was changed depending on the characteristics of the carbon source. The ammonia nitrogen concentration was the highest in the starch-treated group at 7, 12, and 15 days after incubation (P < 0.05). Cumulative volatile solids reduction was the highest in the cellulose-treated group at 18 day after incubation (P < 0.05) and cumulative gas production was higher in the cellulose-treated group than for other two treatments at 18 day after incubation (P < 0.05). Methane content was the lowest in the xylan-treated group at 18 days after incubation (P < 0.05). Cumulative methane production was higher in the xylan-and cellulose-treated group than in the starch-treated group at 18 days after incubation (P < 0.05). In this study, the carbon sources had significant effects on anaerobic fermentation characteristics; especially, the carbon source was shown to have a positive effect on the operation time and hydraulic retention time for the anaerobic digestion startup phage. Therefore, carbon sources should be considered systematically for efficient anaerobic digestion of organic waste.

생물 반응기를 이용한 현삼 종묘의 생산에 있어 적정 탄소원과 질소원의 종류 및 농도와 , 생물 반응기 형태에 대한실험의 결과는 다음과 같다. 탄소원으로서 과당을 사용하였을 때 자당 사용의 경우보다 다신초의 형성은 좋았으나, 비정상적인 개체가 많아 종묘 생산용으로는 부적합하였다. 질소원으로 질산태 질소의 효과적 이었으며, 암모니아태 질소 413 mg/L 에 절산태 질소 1,900 mg/L 로 조합하는 것이 신초형성에 가장 적합하였는데, 500 ml 플라스크 배양에서 전 질소 함량은 3 주째 까지는 83% 에서 75% 로 완만하게 감소하다 이후 46% 로 급격히 감소하였다. 이러한 현상은 신초가 급속히 증가하는 시기와 일치하였고 생물 반응기에서는 일정한 속도로 감소되었으며, 배양 종료시인 6주 째에는 500ml 플라스크에서나 생물 반응기 배양액 내의 질소함량은 거의 남아 있지 않았다. 생물반응기의 배지내 자당은 2주째에 완전히 고갈되었으며 500ml 플라스크 배양에서 자당은 3주째에 완전히 고갈되었고, 자당에서 전환된 과당과 포도당은 절편체의 생체중이 급격히 증가되는 4주와 5 주 사이에 감소되었다. 현삼의 배양에 적합한 생물 반응기 형태는 inner loop 가없는 air lift형 sphere type이었다.

지황(Rehmannja glutjnosa)에서 종근 저장시 나타나는 병원균의 오염을 배제하면서 종묘를 대량 증식시키는 방법을 개발하기 위하여 액체 배양을 통해 직접 체세포배를 형성하고자 할 때 요구되는 배지, 질소원, 탄소원 및 pH와 배양 기간 중 배지의 성분 변화를 조사하였다. 줄기나 엽병을 접종조직으로 할 경우, 배지내 질소원인 NH4NO3 와 KNO3의 적정비율은 825 mg/1 : 1900 mg/l 이었고, 탄소원으로는 자당이 적합하였으며, 적정 농도는 3% 이었다. 배지로는 1X MS 배지가 적합하였고, 적정 BA 농도는 2.0 mg/l 였는데, NAA와 조합하였을 경우에는 BA 1 mg/l에 NAA를 0.5 mg/l 를 조합한 배지 와 BA 2.0 mg/l 에 NAA를 0.1 mg/l로 첨가한 배지가 체세포배 발생에 효과적이었다. 멸균 전에 pH를 5.7로 조정한 경우에 직접 체세포배의 형성에 효과적이었다. 배양기간 중 배지 내 Ca++와 Mg++이온은 거의 변화가 없었으나, K+이온은 많이 흡수되었다. 배양 3주 째에 자당은 거의 고갈되었고, 과당과 포도당은 3주 째부터 흡수되기 시작하였다.

본 연구에서는 탄소원, 질소원의 비율, inositol 및 비타민이 박하정유 생산과 멘톨생합성에 미치는 영향을 조사한 결과 암모니아태질소와 초산태질소의 비율이 1:2일 때 사용한 배지에 관계없이 정유생산이 많았다. 멘톨 생합성이 높았던 경우는 LS배지에서는 암모니아태와 질산태질소의 비율이 1 : 3이었고 MS배지에서는 1 : 2이었다. Sucrose 대신 glucose를 사용하였을 때는 사용한 배지에 관계없이 정유생산과 멘톨 생합성이 모두 감소하였다. Inositol의 농도를 증가하였을 경우 세포의 생장량은 증가하였으나 멘톨 함량은 감소하였다. 비타민 농도를 기본배지의 두배로 하였을 때 LS배지에서는 정유함량이 증가하였으나 MS배지에서는 감소하였다. 멘톨 함량은 모두 감소하였다.