Curcumin is a hydrophobic polyphenol extracted from turmeric that exhibits a variety of biological functions has albeit with limited efficacy as a functional food material owing to its low absorption when administered orally. The newly developed curcumin powder formulation exhibits improved absorption rate in vivo. This study evaluates the anti-oxidant effects of Theracurmin® (TC), which is highly bio-available in curcumin powder. The antioxidant activity of TC was investigated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, ferrous reducing antioxidant power (FRAP) assays, NO radical, superoxide radical, H2O2 scavenging activity, and total antioxidant capacity (TAC). Additionally, we evaluated the antioxidant activity of TC in high-fat diet (HFD)-fed streptozotocin (STZ)-induced Type 2 diabetic rats. As a result of oral administration of TC for 13 weeks in type 2 diabetic rats, the group administration of 2,000 mg/kg significantly increased FRAP, superoxide dismutase (SOD), and reduced the level of glutathione (GSH) in liver tissue 1.9, 1.2, and 1.2-times, respectively. Furthermore, serum TAC levels increased by 1.3-fold after the rats were administered with a dose of 500 mg/kg. These results were consistent with the in vitro assay results. In conclusion, TC exhibited its potential as a functional food material through its antioxidant properties.
Recently, the existence of non-neuronal, soluble AChEs with non-classical functions, such as stress response and chemical defense, has been reported in both vertebrates and invertebrates. With this in mind, it is intriguing to hypothesize that fat body is a main tissue to express non-neuronal AChE at least in some insects. As an initial step for the systematic approach to investigate the distribution of non-neuronal AChEs in insect fat body and to elucidate their physiological functions, we have selected 12 different insect species across different orders and isolated fat body tissues from them. Then, the presence or absence of AChE and its solubility nature were determined by native polyacrylamide gel electrophoresis in conjunction with western blot analysis insect-specific AChE1 and 2 antibodies. Among 12 insects examined, soluble AChE1 was determined to be expressed in fat bodies of insects involving honey bees, brown plant hoppers, dynastid beetles, lice, etc, AChE2 in fruit flies, bed bugs, mealworm beetles. However, no AChE was detected in fat bodies of the remaining two species American cockroaches and dragonflies Our findings clearly show that AChE is widely distributed in the fat body tissue of diverse insect species. More extensive investigation on in a wider variety of insect species would be necessary to deduce the evolutionary origin of fat body-specific AChE, which would be the ancestor of AChE with non-neuronal function.
데니쉬 페이스트리의 유지는 반죽과 결합하여 수분증발속도를 늦춰주고 부피를 증대시키는 효과가 있으며 유지의 종류에 따라 데니쉬 페이스트리의 품질이 다르다. 유지의 종류에 따라 다른 데니쉬 페이스트리의 특성을 비교한 결과, 쇼트닝을 충전용 유지로 이용한 데니쉬 페이스트리는 가장 작은 부피와 가장 큰 경도와 점탄성을 보여주었으며 단면을 잘라 비교하였을 때 상대적으로 크고 불규칙한 기공을 나타내었다. 반면 버터를 충전용 유지로 이용한 데니쉬 페이스트리는 비교적 큰 부피와 작은 경도를 갖는 것을 보아 매우 부드러운 질감을 가졌으며 조밀하고 균일한 크기의 기공을 나타냈다. 따라서 버터, 마가린, 쇼트닝을 이용하여 데니쉬 페이스트리를 제조 시 버터를 이용하였을때 가장 좋은 품질의 데니쉬 페이스트리를 제조할 것으로 기대된다.
This study was conducted to compare the carcass characteristics of native type black pigs (Black pigs) with those of commercial cross bred pigs (Commercial pigs) with the data collected from pig farms. 8364 and 33786 records were collected from Black pigs and the Commercial pigs, respectively. The Commercial pigs were mainly from three breed crosses of Yorkshire, Landrace and Duroc. Commercial pig data was subdivided into manual and machine sub data set on the judging method. Black pigs showed lower carcass weight (CWT) and thinner back fat thickness (BFT) than Commercial pigs. However, the ratio of BFT to CWT (BFT/CWT ratio) was higher in Black pigs than Commercial pigs. The carcass grades of Commercial pigs were mostly in A and B, C, and D in descending order, while the grades D or E were observed in most carcass of Black pigs. Barrow tended to be thicker in BFT and higher in BFT/CWT ratio than gilt both in Commercial and Black pigs, and gilt were higher in loin depth (LD) and Estimated Lean Yield Percentage (ELP) which were available only in the data by machine evaluation of Commercial pigs. Residual correlation coefficient (+0.496) between carcass weight and back fat thickness in Black pigs did not differ from those in Commercial pigs which were +0.434 and +0.414 in the data by manual and machine evaluation, respectively. The linear regression coefficients of back fat thickness on carcass weight were +0.364 mm in Black pigs, and +0.257 mm (manual evaluation) and +0.236 (machine evaluation) in Commercial pigs, which suggests relative accumulation rate of BFT to the growth of weight was faster in Black pigs than Commercial pigs.
본 연구는 섬더덕의 항비만 효능과 지질대사 개선효과를 알아보기 위해 C57BL/6J 4주령의 마우스에게 고지방식이를 9주간 급여하여 비만을 유발한 후 섬더덕 추출물을 첨가한 식이를 5주간 먹여 실험하였다. 체중은 고지방식이군에서 유의적으로 증가하였으며, 섬더덕 추출물을 먹인 마우스에서 유의적인 감소를 하였다. 식이효율 결과 또한 섬더덕 추출물을 공급한 군이 고지방식이군보다 통계적으로 유의하게 낮은 것으로 나타났다. 장기 및 지방무게에서도 고지방식이군에서 무게가 증가한 것을 확인하였고, 섬더덕 추출물을 공급한 군에서 장기 및 지방의 무게가 전반적으로 감소한 것을 알 수 있었다. 혈장을 이용한 중성지방, 총콜레스테롤, 고밀도콜레스테롤을 측정한 결과 섬더덕 추출물을 공급한 군에서 중성지방과 총콜레스테롤은 유의적으로 감소하였고, 고밀도콜레스테롤은 유의적으로 증가함을 확인 할 수 있었다. 동맥경화 지수와 심혈관계 위험도 지수는 감소된 것을 확인할 수 있었다. Western blot을 통해 지방관련 단백질 발현양상을 본 결과 지방분해에 관여하는 PPAR α의 경우 섬더덕 추출물을 급여함으로써 발현양이 증가하였으며, 지방생성 관련 단백질인 SREBP-1, FAS, ACC의 경우 고지방식이군과 비교 시, 섬더덕 추출물을 공급한 군에서 억제되었다. 이상의 결과로 섬더덕 추출물의 섭취는 체중 감소와 더불어 혈장 지질수준 개선에도 도움을 줄 수 있음을 제시하였다.