Oral bacterial infections substantially affect the development of various periodontal diseases and oral cancers. However, the molecular mechanisms underlying the association between Fusobacterium nucleatum (F. nucleatum ), a major periodontitis (PT)-associated pathogen, and these diseases require extensive research. Previously, our RNAsequencing analysis identified a few hundred differentially expressed genes in patients with PT and peri-implantitis (PI) than in healthy controls. Thus, in the present study using oral squamous cell carcinoma (OSCC) cells, we aimed to evaluate the effect of F. nucleatum infection on genes that are differentially regulated in patients with PT and PI. Human oral squamous cell carcinoma cell lines OSC-2O, HSC-4, and HN22 were used. These cells were infected with F. nucleatum at a multiplicity of infection of 100 for 3 hours at 37℃ in 5% CO2. Gene expression was then measured using reverse-transcription polymerase chain reaction. Among 18 genes tested, the expression of CSF3, an inflammation-related cytokine, was increased by F. nucleatum infection. Additionally, F. nucleatum infection increased the phosphorylation of AKT, p38 MAPK, and JNK in OSC-20 cells. Treatment with p38 MAPK (SB202190) and JNK (SP600125) inhibitors reduced the enhanced CSF3 expression induced by F. nucleatum infection. Overall, this study demonstrated that F. nucleatum promotes CSF3 expression in OSCC cells through p38 MAPK and JNK signaling pathways, suggesting that p38 MAPK and JNK inhibitors may help treat F. nucleatum-related periodontal diseases by suppressing CSF3 expression.

본 연구의 목적은 종합병원 간호사의 임파워먼트와 직무소진이 감염관리 수행에 미치는 영향요 인을 확인하기 위한 서술적 조사연구이다. 대상자는 D시에 소재한 종합병원에서 근무하고 있는 간호사 총 210명을 대상으로 2023년 9월 15일부터 10월 31일까지 자료를 수집하였다. 수집된 자료는 SPSS/WIN 25.0 프로그램을 이용하여 기술통계, t-test, ANOVA, Sheffe, Pearson’s correlation, multiple regression을 이용하여 분석하였다. 연구결과, 임파워먼트와 감염관리 수행은 양의 상관관계를 보였으며, 임파워먼트가 감염관리 수행에 가장 영향을 미치는 요인으로 분석되었으며, 14.8%의 설명력을 보였다. 따라서 간호사의 임파워먼트를 향상시킬 수 있는 효율적인 감염관리 수행을 위한 프로그램 및 정책반영이 필요하다.

The primary therapeutic approach for Brucella species infections has mainly been based on antibiotic treatment. However, the development of vaccines for brucellosis control remains controversial. Furthermore, there is currently no licensed vaccine available for human brucellosis. This study aims to evaluate the effect of a combination of recombinant protein vaccines against Brucella (B.) abortus infection using a mouse model. Two B. abortus genes, namely dapB and gpm, were cloned and expressed in competent Escherichia (E.) coli DH5α using the pCold-TF vector. Successfully cloned vectors were subjected to PCR amplification using specific primer pairs. The apparent sizes of dapB and gpm were detected at 807 bp and 621 bp, respectively. Besides, the purified recombinant proteins dapB and gpm were detected using SDS-PAGE electrophoresis with correct sizes of 82.86 kDa and 87.61 kDa, respectively. These recombinant proteins were used to immunize mice as a combined subunit vaccine (CSV) to elicit host immunity against B. abortus infection. Mice immunized with CSV exhibited increased proliferation of CD4+ and/or CD8+ T cells at week 7th and 9th before sacrifice, in comparison to the control group. Notably, CSV immunization showed a significant decrease in bacterial burden in the spleen compared to the control group. Altogether, CSV using dapB and gpm induced host adaptive immune response against Brucella infection, suggesting its potential as an effective new subunit vaccine candidate.

본 연구는 국내에서 재배되고 있는 절화 장미 품종들에 있 어서 바이러스 감염 여부를 검정하기 위해 실시하였다. 절화 장미의 주산지인 경기도 G시의 장미재배 농장에서 8품종을, 경상남도 C시의 장미재배 농장에서 10품종을, 전라남도 H군 의 장미재배 농장에서 14품종을, 전라북도 J군의 장미재배 농 장에서 9품종 등 총 41품종을 대상으로 ArMV, INSV, TMV, ToRSV, TRSV, TSV, TSWV 등 총 7종류의 바이러스에 감염되었는지를 ImmunoStrip® kit를 이용하여 검정하였 다. 경기도 G시의 장미 재배농장의 ‘Bobos’, ‘Pink Army’, ‘15-712’, ‘18-356’은 TRSV에 감염된 것으로 나타났고, 경상 남도 C시의 장미 재배농장의 ‘Haesal’은 TMV, ‘Querencia’ 는 ToRSV에 감염된 것으로 나타났다. 전라남도 H군의 장미 재배농장의 ‘Deep Purple’은 ArMV와 TRSV에, ‘Lady of Shalott’은 TRSV, ‘Mortimer Sackler’는 TMV와 TRSV, ‘Natal Briar’는 ArMV와 INSV에, ‘Sprit of Freedom’은 INSV, ‘Imogen’은 TRSV, ‘Pink Beauty’는 ArMV, TMV, TRSV에 감염된 것으로 조사되었다. 전라북도 J군의 장미 재배농장의 ‘Amor Zen’, ‘Dignity’, ‘Peach Pangpang’, ‘Yellow Pangpang’ 등 4개 품종은 TRSV에 감염된 것으 로 나타났고, 본 연구에서 조사한 총 41개 품종 중 22개 품 종이 바이러스에 감염되었으며, TRSV에 13 품종이, ArMV와 TMV에 3 품종이, INSV에 2 품종이, ToRSV에 1 품종이 감 염되어 TRSV에 가장 많이 감염된 것으로 나타났다.

Extensive research and testing continue to be conducted for the development of vaccines targeting zoonotic diseases such as brucellosis. In this study, the potential of the DapB as a recombinant protein vaccine to effectively combat Brucella abortus 544 infection in BALB/c mice was evaluated. Western blotting assay results showed that recombinant protein DapB reacted with Brucella-positive serum, indicating its potential immunoreactivity. In vivo results showed that the peripheral blood CD4+ and CD8+ T cell population significantly increased in the DapB-immunized mice group after the first, second and third blood collection, compared to the control group that received PBS. Additionally, at the fourth blood collection, an increase in CD4+ T cell activation was observed in three vaccination groups compared to PBS negative control group. These results indicate the potential of DapB in stimulating cellular immunity. Fourteen days after infection, the bacterial load in the spleen was evaluated. The reduction in bacterial replication in the spleen by both DapB and RB51 highlights their protective efficacy against Brucella infection. These findings contribute to the ongoing efforts in developing effective vaccines against brucellosis and provide valuable insights for further research in this field.

Purpose: This study aimed to understand the in-depth experiences of infection control liaison staff (ICLS) in hospitals. Methods: This qualitative study applied Colaizzi’s phenomenological research method and targeted 12 healthcare workers in various occupations with experience as ICLS in the departments of a general tertiary hospital in Seoul and a general hospital in Gyeonggi-do. Data were collected through individual in-depth interviews from February 21, 2022, to April 30, 2022, and data analysis was conducted in a circular structure based on Colaizzi’s phenomenological research method. Results: A total of 32 themes and 14 theme clusters were derived from 132 meaningful statements, resulting in six categories. The six categories are “A prepared mind to infection control”, “The initial burden weighing on one’s shoulders”, “Facing difficulties due to inadequate work support”, “Overcoming with cooperation and rewarding”, “The transformed patient and work environment in my journey of personal growth”, and “Finding support measures for ICLS”. Conclusion: The results of this study are, expected to help understand the meaning and value of ICLS in medical institution departments. Based on this, it will be possible to contribute to preparing primary interventions for education and capacity development, support measures, and system improvement for infection control personnel in medical institution departments.