Light plays an important role in fruit-body development and morphology during Pleurotus spp. cultivation. To understand the effects of light color on fruit-body properties, we evaluated the fruit-body characteristics of Pleurotus spp. varieties cultivated under blue, red, and purple LED light sources. The main results are as follows: The overall fruit-body shape showed differences depending on the color of the LED light. The fruit-bodies of mushroom cultivated under blue and purple light were generally similar to the mushroom shapes typically produced, while those of mushroom cultivated under green light were abnormally shaped, probably due to the absence of effective light source. The average cap lightness of mushrooms cultivated under blue, green, and purple LED lights was 57.0, 57.4, and 59.4, respectively. The average cap lightness of all varieties except Wonhyeong1ho and Hwang-geumsantari cultivated under the three LED light sources were statistically significantly different (P<0.05). The cap redness varied significantly depending on the LED lighting and variety. Only Gonji7hoM, the cap color mutant of Gonji7ho, showed negative cap redness values under all three LED light sources. Among the eight varieties excluding Gonji7ho, the highest cap redness was observed when cultivated under the blue LED. The average harvest weight of the varieties cultivated under purple, blue, and green LED light were 68.0, 58.3, and 50.1 g, respectively. The yield of Gonji7ho, the mushroom variety with the highest yield, cultivated under blue, green, and purple LED light were 92.8, 77.1, and 98.6 g, respectively. The earliness when grown under the purple, blue, and green LED lights were 5.3, 5.8, and 5.8 days, respectively. Among the varieties, six, three, and two cultivars showed the shortest earliness under the purple, green, and blue LED, respectively. The fruit-body lengths were 66.4, 51.8, and 46.8 mm when cultivated under green, purple, and blue lights, respectively. These results are expected to serve as a foundation for producing mushrooms with traits demanded in the market.
본 연구는 콜레마니진디벌의 품질확인장치에 적용할 수 있는 LED 광원을 선발하기 위하여 수행되었다. 4개의 LED 광원(385, 405, 450, 및 660 nm)을 대조구로 5,000k 백색 LED를 사용하여 Y자관 시험에서 선택시험을 수행한 결과, 385 nm LED 광원만 콜레마니진디벌에 대한 유인효과가 있었다. 하지만 3개의 LED 광원(385, 405, 및 450 nm)과 대조구로 5,000k 백색 LED를 사용하여 비선택시험을 수행한 결과, 450 nm > 385 nm > 405 nm 광원 순으로 유인효과가 높았으나 450 nm와 385 nm 광원간 유의차는 없었다. UV-A에 속하는 385 nm LED 광원은 피부질환을 초래할 수 있는 우려가 있어 제외하였고, 최종 450 nm LED 광원을 콜레마니진디벌 품질확인장치에 적용할 광원으로 선발하였다. 450 nm LED 광원을 콜레마니진디벌 우화기구에 적용한 결과, 87.4%의 높은 유인효과를 확인 할 수 있었다. 이러한 결과는 천적 품질관리에 유 용하게 활용될 수 있을 것으로 생각된다.
인공광 이용형 식물공장에서 인공광원의 전력 소모는 작물 생산 비용을 증가시키는 주요한 요인이다. 따라서 인공광원의 광 이용효율을 향상시키는 것은 식물공장의 경제성 확보에 중요한 사안이다. 본 연구의 목적은 다양한 스펙트럼을 갖는 백색 LED와 원적색광을 추가한 다양한 백색 LED에서 재배 된 이고들빼기의 생산성과 광 이용효율을 비교하는 것이다. 파종 후 3주된 이고들빼기 묘는 심지 이용 수경재배 시스템에 정식되었고, 기온 20 ± 0.1°C, 상대습도 60 ± 0.1%, 이산화탄소 938.2 ± 5.8μmol·mol -1 , 광도 PPFD 250.6 ± 0.6μmol·m -2 ·s -1 , 광주기 16시간으로 설정된 인공광 이용형 식물공장에서 5주 재배되었다. 광질 처리조건은 대조구로 식물재배용 3가지 백색 LED(CL 1, CL 2, CL 3), 백색 LED 그룹[Warm W(WW), Neutral W(NW), Neutral W + Red(NWR), Cool W(CW)], 원적색광이 추가된 백색 LED 그룹(WWFR, NWFR, NWRFR, CWFR)이 사용되었다. 백색 LED에 원적색광의 추가는 지상부 생육특성 항목(생체중, 엽장, 엽면적)을 증대시켰고 WWFR 의 지상부 생장이 유의적으로 가장 높았다. 반면에 원적색광의 보광은 엽록소 함량을 감소시켰고 플라보노이드 지수에는 영향을 주지 않았다. 재배기간 동안의 총 소비전력은 CL 1에서 가장 낮았고 WWFR의 총 소비전력이 가장 높았다. WW와 WWFR의 광 이용효율이 높았으며 백색 LED에 원적색광의 추가는 광 이용효율을 3–15% 증대시켰다. 따라서, 백색 LED 에 원적색광의 보광은 작물의 생산성과 광 이용효율을 향상시켜 작물의 생산비용을 감소시킬 수 있었다.
적색과 청색광은 식물의 광합성에 효과적인 파장으로 알려져 있다. 본 연구는 다양한 조합의 적색과 청색 LED 혼합광에서 자란 방울 토마토 묘의 생장과 정식 후의 생산량과 품질에 대한 영향을 구명하였다. 파 종 후 2주된 방울 토마토 묘(Solanum lycopersicum L. cv. ‘Cuty’)를 적색(655nm)과 청색(456nm) LED의 다양 한 비율의 혼합광[red:blue = 41:59 (59B), 53:47 (47B), 65:35 (35B), 74:26 (26B), 87:13 (13B), or 100:0 (0B)]과 형광등(대조구)이 설치된 생장상에 옮겨준 후 25/20℃ (주/야), 광합성 광량자속 198.6 ± 5 μmol·m-2·s-1 (12시간)의 조건에서 27일간 육묘하였다. 그 후 방울 토마토를 벤로형 온실에 정식하여 75일동안 재배하였다. 정식 전 육묘 단계에서 27일간 LED 처리된 0B 와 59B 처리구를 제외한 모든 RB 혼합광 처리의 지상부 생 체중이 대조구에 비해 높았다. 지상부 건물중과 엽면적 또한 지상부 생체중과 유사한 경향을 보였다. 줄기 신 장은 13B, 26B, 35B에서 대조구와 다른 처리구들에 비해 유의적으로 가장 높았다. 특히, 26B는 59B처리에 비 해 약 3.2배 높은 줄기 신장을 보였다. 정식 후 37일 째에 마딧수는 26B와 47B에서 유의적으로 가장 높았고, 식물 초장은 26B에서 대조구와 59B에 비해 유의적으로 높았다. 가장 높은 총 과실 생산량을 보였던 26B는 대조구에 비해 1.6배, 59B에 비해 1.8배 높은 총 과실 생산량을 보였다. 따라서, 본 연구는 적색과 청색 LED 의 다양한 혼합 비율이 방울 토마토 묘의 생장 및 발달과 정식 후의 과실 생산량과 같은 생식생장에 영향에 중요한 요소임을 제시한다.
In order to study for the growth of fish in the aquaculture industry, the behavior analysis of rock bream (Oplegnathus fasciatus) depending on the temperature and LED lights was conducted. In this study, water temperatures from 10 to 30 degrees were used. One red light (wave length: 622 nm; light power: 811 mW) and one green light (wave length: 518 nm; light power: 648 mW) were used. Behavior of the rock bream was analyzed at an average moving distance for one minutes (AMD) and a rate of movement. The mean AMD were respectively 5.3 m, 7.3 m and 3.0 m in the red LED light, green LED light and control condition. The mean rates of movement were 77%, 88% and 61% respectively in the red LED light, green LED light and control condition. The mean AMD during 24 hours were 3.1 m, 3.1 m and 3.3 m respectively in the red LED light, green LED light and control condition.
In order to establish the basic data for the growth of fish in the aquaculture industry, the behavior analysis of rockfish (Sebastes inermis) depending on the temperature and LED lights was conducted. In this study, water temperatures were set from 3℃ to 30℃ were used. One red light (wave length: 622 nm; light power: 811 mW) and one green lights (wave length: 518 nm; light power: 648 mW) were used. Behavior of the rockfish was expressed as average moving distance (AMD) for 1 minutes and a rate of movement. The mean AMD depending on the temperature was 1.0 m and the mean rates of movement was 50%. The mean AMD were 1.5 m, 1.9 m and 0.7 m in the red LED light, green LED light and control condition respectively. The mean rates of movement were 54%, 65% and 45% in the red LED light, green LED light and control condition respectively.
Light-emitting diodes are used in applications as diverse as aviation lighting, automotive lighting, advertising, general lighting, and traffic signals. LED panel light source used in an experiment on plant and mushroom growth. To establish the optimum artificial light illumination method for Pleurotus eryngii in closed mushroom factory system, the effects of red LED light quality on growth was investigated. The humidity was maintained more than 90% and temperature was 15~16℃, and CO2 density was 500ppm. The fresh weightand height were higher under red LED light (650nm, 73.1 Pmol/m 2s) comparing with control (fluorescent lamp, 10~20 lux). β-aminoisobutyric acid and total phenol content were higher under red LED light (650nm, 73.1 Pmol/m2s).
To establish the optimum artificial light illumination method for Pleurotus eryngii in closed mushroom factory system, the effects of red/blue LED light quality on growth was investigated. The following parameters fresh weight, height, width, chromaticity and hardness of the pileus were considered for studying the effect of LED light on the growth of mushroom. Pleurotus eryngii : The humidity was maintained more than 90% and temperature was 15~16℃, and CO2 density was 500ppm. The fresh weight and height were higher under red LED light (650nm, 73.1 Pmol/m2s), green LED light (525nm,89.1Pmol/m2s) comparing with blue LED light (450nm,105.8Pmol/m2s).
LED 광원을 달리하여 재배한 감초지상부의 항산화, 항돌연 변이원성을 평가하였다. BL-0에서 DPPH 라디칼 소거활성에 서 EC50이 3.02 ± 0.13 ㎍/㎖와 ABTS 라디칼 소거활성은 EC50 이 2.18 ± 0.18 ㎍/㎖와 환원력이 가장 높게 나타났다. 폴리페놀 함량은 2.93 ± 0.11 g/100 g으로 가장 높게 나타났으며, RL-0 에서는 항산화 활성이 가장 낮게 나타났다. 항돌연변이원성 평 가에서 직접변이원인 1-NP에 대해 모든 실험군에서 돌연변이 억제효과를 나타냈으며, 간접변이원인인 Trp-P-1, Trp-P-2, AFB1에 대해서는 BL-0에서 가장 높은 돌연변이 억제효과를 나 타냈고, 2-AA에서는 RL-1에서 돌연변이 억제가 높게 나타났 다. 청색광 LED (650 ㎚)와 적색광 LED (450 ㎚)를 이용하여 재 배한 감초지상부의 성분변화를 관찰하기 위해 GC/MS를 이용 해 분석한 결과 25분에서 RL-1에서만 cytidine이 검출되었고, 37분에서 BL-0에서만 2-bromotrimethylene glycol이 특정적 으로 검출되었다. 이는 LED광원 조건에 따라 감초 경엽의 성분 이 차이가 있음을 보여준다. 이 성분들이 생리활성에 어떤 영향 을 주는지는 더 검토해야할 부분이다.
The food functional properties of Pleurotus eryngii cultivated with different wavelengths of light-emitting diode (LED) light were analyzed in this study. The levels of total soluble solids of Pleurotus eryngii increased with all the LED lights, except mixed LED light (B*R, B*G and R*G). Thirty one kinds of components were detected by analyzing the free amino acids. The total free amino acid contents had a slightly higher tendency in the control group, but the ratios of eight essential amino acids among the total amino acids of Pleurotus eryngii cultivated under all the LED lights, including the blue light (blue, B*R, B*G and B*R*G*U) and the green light, were higher than that in the control group. K was the highest-content of mineral, and Mn was the lowest-content. The Ca content increased through cultivation under all the LED lights, except the blue light, but the Fe content (under the green and B*R lights), K content (under the red and UV-A lights) and Mg content (under the R*G, UV-A and red lights) increased under the exclusive LED light. The total phenolic compounds increased by cultivation under all the LED lights, except the UV-A and mixed lights (R*G and B*R*G*U). The DPPH radical scavenging activity was also improved by all the LED lights, except the mixed light (R*G).
The effect of the wavelength of the light emitted by the light-emitting diode (LED) on the growth characteristics and physicochemical and sensory qualities of Pleurotus eryngii were investigated. Pleurotus eryngii were grown under different light sources: blue light (450 nm), red light (650 nm), green light (525 nm), UV-A (365 nm), and mixed light (B*R, B*G, R*G, B*R*G*UV-A). The quantity of LED light was set up at 50% (LED: 64.9-108.0 p㏖m-2·s-1;fluorescentlight:11.7lux). Fluorescent light was used as control. There were no significant differences in the flesh firmness. In the case of the Pleurotuseryngii cultivated under red, green, and mixed light (R*G), the color of the pileus and the length of the stipe were similar to those of the control group. The sensory scores were not significantly different between the LED lights (red, green, and R*G) and the control. Among the three LED light conditions, the sample cultivated under red light recorded the highest score. The samples under UV-A, blue, and mixed light (B*R, B*G, B*R*G*U) had a dark pileus color and had a short stipe. These results showed that the wavelength of LED light affected the growth and quality characteristics of Pleurotus eryngii, and that using red LED light is preferable for the cultivation of Pleurotus eryngii with better quality.