분말을 우리밀 밀가루 중량 100%를 기준하여 각각 0.5, 1.0 및 2.0% 첨가하여 제조한 국수의 품질특성을 조사하였다. DPPH radical 소거활성, 환원력, 자동산화 억제활성, tyrosinase 저해활성을 측정한 결과, 분획물의 농도가 증가함에 따라 항산화활성은 증가하는 경향을 보였으며, 부탄올 분획물에서 높은 DPPH radical 소거활성, 환원력, 자동산화 억제활성, tyrosinase 저해활성을 보였다. 솔잎분말을 첨가하여 제조한 국수의 중량, 부피, 함수율 및 국물의 탁도는 대조구와 비교하여 분말의 첨가량이 많을수록 증가하였다. 색도는 솔잎분말의 첨가량이 증가함에 따라 L값과 a값은 감소하였으며 b값은 증가하였다. 솔잎분말을 첨가하여 조리한 국수의 조직감은 우리밀 밀가루만 사용하여 제조한 대조구에 비하여 경도, 응집성, 탄력성, 검성 및 씹힘성이 감소하는 경향을 보였다. 관능검사 결과 솔잎 분말 1.0% 첨가구에서 색, 맛 및 전체적인 기호도가 높게 나타났으며, 향과 조직감에서는 대조구와 비교하여 유의적인 차이를 나타내지 않았다.

The pine needles can be used for four seasons in normal living and it can be taken friendly everywhere as it is distributed over 50% in Korea. The pine needles consist of vitamins, protein, minerals, essential oil and enzyme related to antimicrobial activity. It has effect like high blood pressure, neuralgia and hanged over by terpene, glucokinin, rutin, apigenic acid and tannin. Also the extract of them can be used for dyeing of fabrics. However, the extract components and effects of them are not well known yet. Therefore, the purpose of this study was to investigate the volatile components of the pine needles extract and functionality. The pine needles extract was dyed into various fabrics(nylon, silk, wool and soybean) and mordanted with Al, Cu, Cr, Fe and Sn. The extracted aroma compounds were compared by gas chromatography-mass spectrometry. The major volatile compounds of pine needles verified by using SPME were alpha-pinene, beta-pinene, beta-phellandrene, caryophyllene, ethanon, benzen. A total of 15 compounds were identified by using the SPME fibers. In the UV-visible spectra, the maximum absorption of wavelength of the pine needles ethanol extract appeared at 460, 630nm for chlorophyll component and at 237, 281nm for tannin component with the pine needles distilled water extract. Most of sample showed high antibacterial effect in none mordant but wool fabric showed high antibacterial effect in mordants. The result of UV block test showed a superior ability of blocking ultraviolet ray infiltration in all sample.

The pine needles has been used as medicines and it is using as dyeing as well as food. It is distributed through 50% in Korean forest. The pine needles is related to antimicrobial activity, however, dyeing properties of the extract components and effects of them are not well known yet. To examine dyeing properties of the pine needles extract, various fabrics(nylon, silk, wool, and soybean) were dyed under different dyeing conditions, and mordanted with one of five mordants(Al, Sn, Fe, Cr, and Cu). Dye uptake, Colors and Colorfastness of the dyed fabrics were measured. By the K/S values, dye uptake of all the dyed fabrics enhanced as increasing dyeing temperature and dyeing time. The highest K/S values were obtained from the protein fiber(wool and silk) fabrics dyed with water extract at 100℃, and with ethanol extract at 90℃ for 80 minutes. Colors of the dyed fabrics showed variety of yellow, brown and green colors. Colors changed by using Cu, Fe and Cr mordants: Cr-mordanted fabrics into light green, Fe-mordanted fabrics into reddish brown, and Cu-mordanted fabrics into deep green in ethanol extraction. Dry-cleaning fastness of the dyed fabrics presented good to excellent except wool fabrics dyed with ethanol extract. Washing fastness of the dyed nylon and soybean fabrics were good to excellent but wool and silk fabrics showed average grades. Most dyed fabrics were poor to light fastness.

We extracted pine-needles using ethanol as solvent, and we obtained the refined oil component from pine-needles extract. Also we tested the tyrosinase activated inhibition effect with melanin experiment and analysed with ICP/OES and UV/VIS. Accordingly we obtained the next conclusion from the result of this experiment. From the first result of this experiment, we could know that the degree of recovery of refined oil component from pine-needles extract appeared in about 8.0%. From the second result of this experiment, we could know that the tyrosinase activated inhibition rate increased more and more in case of increasing concentration of pine-needles, green-tea, vitamine-C. Also we could know that vitarnine-C influences to tyrosinase activated inhibition contained in pine-needles. From the third result of this experiment, we could know that inorganic materials of Ca, Mg, V, Mn, etc contained in pine-needles detected with ICP/OES analysis, and the absorbance of pine-needles extract appeared very high in UV/VIS analysis.

We have extracted the natural pine-needles to use ethanol in solvent, and could obtain the refined oil component from pine-needles extract. We have tested the antimicrobial effect from microbe experiment and analyzed with ICP/OES, GC/MS. we could obtain the next conclusion from the result of this experiment. In the first result of this experiment we could know that in case of increasing concentration of pine-needles, the number of microbe decreased more and more. Also we could know that the refined oil component of pine-needles appeared the sterilization effect of S-aureus and E-coli after 96hrs and 120hrs. So we could know that the refined oil component of appeared effect to microbe. In the second result of this experiment we could know that inorganic materials of Ca, Mg, V, Mn etcs from pine-needles detected to ICP/OES analysis and the aromatic compounds from refined oil component of pine-needles was certificated to GC/MS analysis.

As a basic study for preparing pine sprout tea, chemical components in pine sprouts and pine needles were analyzed as follows: In proximate composition the contents of most components except for crude fat were different between in pine sprouts and pine needles. Moisture content in pine sprouts was higher than that in pine needles. Calcium and potassium were major minerals contained in pine sprouts and needles, and their contents in pine needles picked in December were higher than those picked in June. Soluble tannin and vitamin C contents in leaf part of pine sprouts were much higher than those in stem part and their contents in pine needles were increased according to their growth. Free sugars like fructose, glucose and sucrose were contained in both pine sprouts and needles, and their contents in pine sprouts were higher in stem part as compared that in leaf part. Although fourteen kinds of amino acids were detected in pine sprouts and pine needles, their contents were extremely low. Amino acid composition between pine sprouts and pine needles was different each other, but major amino acids contained in them were same, those are acidic amino acids such as aspartic acid and glutamic acid. Amino acid contents in pine needles were increased according to the growth. In fatty acid composition in leaf part of pine sprouts, saturated fatty acid contents were higher than unsaturated fatty acid contents, but in stem part unsaturated fatty acid contents were higher. In pine needles the amount of saturated fatty acid was increased with the growth, but the amount of unsaturated fatty acid was rather decreased.

본 연구는 산성비 및 대기오염물질이 수도권지역의 침엽수 잎에 미치는 피해의 조기판단을 위해 피해도지수, 침엽접촉각, 엽내영양물질, 침엽내 wax, 토양의 화학적 성질 등의 항목을 조사.분석하였다. 조사기간은 1990년 6월부터 1년 동안 하였고, 재료는 대기오염에 민감한 수종으로 알려진 독일가문비나무와 전나무를 이용하였으며 연구결과는 다음과 같다. 피해도지수는 1990년 6월에는 무피해지(광릉, 양평), 경피해지(인천, 보라매공원, 서울여대, 안양, 양수리, 과천), 심피해지(남산, 서울시립대, 어린이대공원, 금곡릉)으로 나누어졌다. 그러나, 1990년 12월에는 인천, 과천이 심피해지로 포함되는 등 시간이 경과할수록 피해가 확산되는 경향을 보였다. 침엽의 접촉각에서도 1990년 6월보다 1990년 12월의 값이 감소하였으며, 두수종 모두 1년생잎에서 2, 3년생잎으로 갈수록 접촉각이 낮게 측정되었다. 그리고, 어린묘목일수록 피해가 심하게 나타났다. 피해도지수와 접촉각간에는 고도의 상관관계가 인정되었다. 엽내영양물질분석에서 Ca, Mg, K의 값은 대체로 시간경과에 따라 감소하였으며, 엽령이 높을수록 낮은 값을 보였다. Wax정량분석은 심피해지역에서는 엽령이 높을수록 wax함량이 적었고, 무피해지역에서는 엽령이 높을수록 많았다. 본 연구결과에 의하면 위 항목의 분석방법들이 상호보완적으로 적용되고, 장기간 지속적으로 수행될 경우 보다 좋은 결과를 얻을 수 있을 것이라 판단되었다.

The needles and pollen of Pinus densiflora and Pinus koraiensis were studied for their lipid contents and fatty acid composition. The total lipid contents in needles of Pinus densiflora and Pinus koraiensis were 5.0 and 4.5%, whereas in pollen of Pinus densiflora and Pinus koraiensis 3.5 and 5.6%, respectively. Twenty-four fatty acids ranged from lauric acid to docosahexaenoic acid(22:6Ω3) were identified in the needle lipids. In needles, linolenic acid and palmitic acid were the major fatty acids. The needles of Pinus densiflora showed higher proportions of docosahexaenoic acid and 5-olefinic nonmethylene-interrupted polyenoic acids than those in the Pinus koraiensis. Twenty fatty acids ranged from myristic acid to lignoceric acid were identified in the pollen lipids. Linoleic acid was the major fatty acid in the pollen followed by oleic and palmitic acid. The fatty acid profile of pollen of Pinus densiflora was similar to those of the Pinus koraiensis pollen lipids.

In order to investigate the effect to antioxygenic substances in Pine Needles on the photooxidation of linoleic acid (linoleic acid 100mg/10ml ethanol) added antioxidants and antioxygenic substances in Pine Needles was irradiated by the tungsten lamp attached with red fitter. The Photo oxidation of linoleic acid (LA) was conformed with Lea method and rhodan method. The following results were obtained: 1. Photooxidation of LA was greatly increased the presence of photosensitizer. However the Photo oxidation of LA without photosensitizer was smoothly increased by the irradiation. 2. The Photo oxidation of LA without irradiation occured quite lately whether photosensitizer was present or absent. 3. Photooxidation of LA under the presence of photosensitizer was inhibited by the addition of dl-α-tocopherol and the acetone fraction of methanol extract of Pine Needles but inhibited by BHT. Photooxidation of LA increased gradually as the addition of BHT increased but decreased gradually as that of acetone fraction increased.

KS F 2782(숏크리트용 급결제) 규격은 터널시공 또는 단면보수․보강 현장에서 사용되는 숏크리트용 급결제의 성능을 평가하기위한 규격이라 볼 수 있다. KS F 2782(숏크리트용 급결제) 규격에 의한 급결제의 품질시험방법은 단순히 급결제의 품질만을 확인하기 위해서는 많은 변수들이 존재하고 있다. 또한 현재까지 생산된 급결제 제품들은 실내시험에서는 거의 대부분 품질기준에 만족하지는 못하지만 현장에서 급결제가 숏크리트에 혼합되어 슈팅된 후의 품질은 상당히 만족하는 수준의 제품들이 다수이다. 즉 실내 시험을 통해 도출한 결과를 바탕으로 현장에 적합여부를 판단하기 위해서는 실내 시험과 실제 타설 시의 품질의 연관성을 확인하고 그에 맞는 시험방법을 찾아야 한다고 본다. 따라서 본 연구는 급결제와 숏크리트의 재료의 조건을 다양하게 하여 길모어침에 의한 시험결과와 압축강도를 비교하여 KS F 2782(숏크리트용 급결제) 규격은 급결제의 품질을 확인하기 위한방법으로서 적합성에 대하여 분석하고자 한다. 그 결과로서 KS F 2782규격의 일부만 수정을 한다고 해서 해결될 수 있는 문제점은 아니며 전반적으로 처음부터 철저하게 검토를 하여 국내 현실에 적합하고 실내 시험의 균일성이 보장되면서 현장에 적용 시 충분한 연관성이 있는 보완책이 마련되어야 한다.

Polychlorinated biphenyls(PCBs) and polybrominated diphenyl ethers (PBDEs) were measured in pine needles of Seoul, and Ansung(GyeongGi-do) to investigate composition, source, and regional concentration of PBDEs. Concentration of total PCBs in Seoul and Ansung showed 608, 945 pg/g DW (mean: 777 pg/g DW), respectively, also concentration of total PBDEs(pg/g DW) in Seoul and Ansung showed 7944, 14,255 pg/g DW(mean 11,100 pg/g DW). Tetrachlorinated biphenyls(Tetra-CBs) and Decabromodiphenyl ethers (Deca-BDE) were detected in relatively high concentration compared to other PBDE congeners in Seoul and Ansung. Correlation coefficients(r) of between PCB congeners in pine needles detected of Seoul and Ansung were significant (r=0.85, p<0.01). It suggested that PCBs concentreation in pine needles may originate from secondary sources such as air-plant partition. The similarity of between PBDE congeners profile to those of the technical mixture formulation (Bromkal 70-5DE) suggests a source of this product in these sites.

솔잎의 기능성에 관한 연구를 위하여 솔잎발효추출물(PEE)과 에탄올추출물(PE 80, PE 50)로 효소적 저해활성과 아질산염 소거작용에 대하여 연구하였다. Tyrosinase의 저해효과는 솔잎발효 추출물인 PEE가 솔잎 에탄을 추출물 PE 80과 PE 50에 비해 약 5∼38％정도 저해 활성 효과가 높게 나타났다. XOase 저해효과는 PFE가 62.77％, PE 80이 64.90％, PE 50이 55.91％의 저해율을 나타내었으며, ACE 저