This study investigated the Texture Profile Analysis and Sensory characteristics analysis of 6 different Pork Meat-substituted Soybean Curd (PMSC) products with different gluten-fiber (GF) contentst. PMSC types were PMSC-GF 5%, PMSC-GF 10%, PMSC-GF 15%, PMSC-GF 20%, PMSC-GF 25%, and PMSC-GF 30%. The lightness (ΔL value) of cooked PMSC was decreased, whereas the redness (Δa value) and yellowness (Δb value) of cooked PMSC was not changed. Texturometer (TA XT Plus, England) was used for objective test and hardness, springiness, cohesiveness and chewiness of sensory characteristics were measured. PMSC with GF 5% to 15% were characterized high hardness score but PMSC with GF 20% to 30% were not significantly different. PMSC-GF 30% was the highest score in springiness and PMSC-GF 5% was the lowest. PMSC-GF 25% and 30% were high cohesiveness score but was not significantly different (p<0.05). Fourteen sensory descriptors were developed to evaluate the sensory characteristics of PMSC. The sensory characteristics of PMSC were sweetness, saltiness, springiness, hardness, chewiness, meat flavor, odor, loose particle and so on. Six different PMSC showed no significant difference in the attributes of loose particle. But PMSC-GF 5% and PMSC-GF 10% showed significant difference in the attributes of hardness. PMSC-GF 25% and PMSC-GF 30% showed no significant difference in the attributes of hardness. PMSC-GF 30% was the highest springiness score and PMSC-GF 5%, PMSC-GF 10% and PMSC-GF 15% showed no significant difference in the attributes of springiness. Overall consumer acceptance was surveyed. PMSC-GF 5%, PMSC-GF 10% and PMSC-GF 15% showed no significant difference in the attributes of color. PMSC-GF 25% and PMSC-GF 30% was the highest in the attributes of springiness and high moistureness, taste acceptance and overall acceptance. PMSC-GF 5%, PMSC-GF 10% and PMSC-GF 15% showed no significant difference in overall consumer preference but PMSC-GF 25% and PMSCGF 30% showed significant difference. Consequently it is indicated that texture of cooked PMSC-GF 25% and PMSC-GF 30% is very important sensory characteristics.

Cotesia plutellae bracovirus (CpBV) is a bracoviral polydnavirus and a parasitic factor of an endoparasitioid wasp, C. plutellae, which parasitizes the diamondback moth (DBM) larvae. CpBV genome was reported to consist of 35 circular segments and encode 157 predicted open reading frames (ORFs). This study revisited and re-annotated the CpBV genome. Total ORFs consist of 15 groups of known gene or gene families as well as hypothetical (HP) group. All 74 HP genes are matched to other PDV genes. RNASeq analysis indicates that 147 genes out of 157 predicted ORFs are expressed in the parasitized DBM at the first day or 7 days after parasitization. At the early parasitic stage, CpBV genes annotated as ELP, BEN, E94K, DUFB, PTP, and HP are highly expressed compared to late parasitic stage. Interestingly, highly expressed genes at the late parasitic stages are HPs.

Buzz, squeak, rattle noise that are referred as BSR have become important part in vehicle's quality because vehicle NVH(noise vibration harshness) reduction technology of main parts like engine and drive-line has made great progress except BSR noise. BSR test is progressed by composing many road excitation power spectrum density(PSD) profiles into a composite PSD profile. Shakers which are input by composite PSD profile make time histories(time-acceleration data) by aggregate of a large number of harmonics related with composite PSD profile in general. But when only composite PSD profile is input to shaker, the time histories from shakers exhibits Gaussian distribution characteristics and can't reflect all road excitation PSD profiles. In this study, we search other studies that try to solve the problems occurring when a shaker is input only PSD profile and analyze time histories resulted from BSR test to check ways of shaker's operation.

복숭아 네 품종 ‘오도로끼’, ‘가납암백도’, ‘진미’, ‘장호원황 도’ 신초의 저온순화 및 탈순화 동안 시기별 내한성 변화는 전해질 누출률을 분석하여 나타냈다. 또한 내한성 결정 요인 을 분석하고자 SDS-PAGE를 이용하여 dehydrin 함량 변화를 확인하였으며, 그와 관련된 유전자 발현 분석은 quantitative real-time RT-PCR을 이용하여 수행하였다. 네 품종의 내한성 은 2012년 12월까지 꾸준히 증가하였으며 그 후 2013년 4월 까지 감소하였다. PpDhn1 유전자가 인코딩하는 60kDa의 dehydrin 단백질은 탈순화기(2013년 3-4월)에 비하여 저온순화 기(2012년11월-2013년 1월) 동안 높은 축적이 확인되었다. PpDhn1 유전자와 PpDhn3 유전자 발현양상은 복숭아 네 품 종에서 내한성 변화와 평행하게 나타난 반면, PpDhn2 유전자 는 뚜렷한 시기별 패턴을 나타내지 않았다.

The Physics Outreach Unit at UNSW Australia contributes to the goals of the IAU's Commission 55 by collaborating with established institutions to improve public engagement with science. We aim to not only increase public awareness of astronomy but also ensure the benefits to society of our scientific endeavours are understood. We have found collaborating with like-minded institutions who are working in similar spaces allows both parties to make a larger impact than working alone. For example, our long-term collaboration with the Australian Museum provides the opportunity to engage urban and rural communities with science, audiences to which we do not normally have easy access. To increase our national presence we are exploring new relationships with other institutions, in particular the Astronomical Society of Australia (ASA), in hosting events such as public talks with eminent astronomers, star parties and astronomical workshops. These partnerships help build firm foundations for planning future events, in particular during the International Year of Light 2015.

The effects of different feeding times, using a diet containing 800 ppm betaine, on production, blood profile, and the short-chain fatty acid (SCFA), was investigated using 240 Cherry Valley (Anas platyrhynchos) meat ducks exposed to a scorching heat wave. The animals were randomly assigned to four groups, each of which was replicated three times with twenty ducks per replicate. The experimental period was 42 days for each group. Four groups were assigned into C (heat wave control group without betaine), T1 (ad libitum group fed a diet containing 800 ppm betaine), T2 (diet-restricted group fed twice daily between 05:00-10:00 and 17:00-20:00, using a diet containing 800 ppm betaine), and T3 (night-restricted group, fed from 17:00-10:00, with a diet containing 800 ppm betaine). At 42 days, body weight increased in order of T2, T1, T3 compared to the heat wave control group although. However, these differences were not found between the groups of T1 and T3. The heat wave control group, provided an ad libitum diet without betaine (C), showed an elevated feed conversion ratio compared to the groups fed a diet containing betaine. However, these differences were not found between the groups of T1, T2 and T3. RBC and platelet profiles except for PLT and MPV showed statistically significant differences between study groups fed a diet containing betaine. T2 presented significantly higher blood electrolytes Na+ and Cl- than the other groups. T2 also showed a blood gas level that was generally higher than the other groups. Total SCFA, acetic acid and propionic acid concentration has been the increasing trend in T2, but butyric acid, isobutyric acid and valeric acid concentration has been the decreasing trend in T2 compared to the other groups. It is concluded that the feeding-restricted group, fed two times daily between 05:00-10:00 and 17:00-20:00, with a diet containing 800 ppm betaine may improve growth performance in meat ducks exposed to a scorching heat wave.

This study investigated the pharmacodynamics of betaine on the blood profile and short chain fatty acid levels in meat ducks exposed to heat wave. 400 heads of Cherry valley (Anasplatyrhynchos) meat ducks were completely randomized to 5 treatments (4 repetitions each), and were raised for 42 days. They were grouped into T1 (heat wave control group without betaine), T2 (betaine 400 ppm), T3 (betaine 800 ppm), T4 (betaine 1200 ppm), and T5 (normal control group without betaine). Compared to T1, the betaine addition groups showed higher body weight gain at shipment, with T3 showing the highest significant difference. For hematological indictors measured (red blood cells and platelets), the betaine addition groups showed significantly higher values than the heat wave control group. The pH of the former was lower but their electrolytes (K+, P+, and Cl-) were significantly higher compared to the latter. For blood gas concentration, the former showed a significantly higher value than the latter. For the total short chain fatty acids, acetic acid, and propionic acid, the betaine addition groups and group fed broiler-high temperature diet showed higher values than the heat wave control group. On the other hand, the former showed significantly lower values in butyric acid, isobutyric acid, valeric acid, and isovaleric acid than the latter group. These results suggest that betaine has the pharmacodynamics that mediate heat stress, via the maintenance and control of the blood profile, osmotic pressure, gas concentration, and short chain fatty acid, of meat ducks under heat wave.

본 연구는 온실에 작용하는 풍하중 산정을 위한 설계 풍속을 결정하는데 필요한 자료를 제공하기 위하여 10m이하 높이에서의 풍속을 측정하여 풍속고도분포지수를 산정하고 변화를 분석하였다. 고도에 따른 풍속분포함수 를 결정하기 위한 풍속고도분포지수를 계산하기 위해서 는 5m·s-1 이상의 풍속을 사용하는 것이 타당하다고 판단된다. 농촌 개활지인 부안지역의 고도에 따른 풍속변 화는 지표면으로부터 풍속이 지수함수로 증가하는 우리 나라의 RDC 기준과 일본의 JGHA 기준과 잘 일치하였고 풍속고도분포지수도 0.26으로 기준들에서 제시된 0.25와 거의 동일한 값을 나타내었다. 반면 군위지역의 경우는 풍속고도분포지수가 0.06으로 산정되어 지표면조 도가 클수록 풍속고도분포지수가 증가하는 일반적인 변 화 경향과는 반대로 나타났다. 이는 타워가 주변지대보다 약 2m 가량 더 높은 위치에 설치되었기 때문에 유선의 급격한 변화에 의한 것으로 판단된다. 따라서 일반적으로 농촌 개활지에 설치되는 온실의 설계를 위해 적용 할 풍속고도분포로는 우리나라의 RDC기준과 일본의 JGHA기준에서 제시한 풍속고도분포가 가장 타당한 것으로 사료된다. 부안의 경우 오전 7시 경부터 풍속고도 분포지수가 감소하다가 오후 3시경에 최소가 된 후 다시 증가하여 24시경에 일정해지는 것으로 나타나 시간에 따른 풍속고도분포지수의 일반적인 변화경향과 잘 일치하였다. 부안지역은 형상변수가 1.51로 나타나 간척지인 부안지역의 풍속특성이 제주도 연안지역과 유사한 풍속특성을 가지고 있음을 확인하였다.

본 시험은 맥섬석과 한약제재 첨가급여가 한우거세우의 비육능력 및 혈액성상에 미치는 영향을 구명하기 위해 생후 13개월령의 거세한우 40두(4처리×5두×2반복)를 공시하여 사료용 첨가제로 1일 사료급여량의 0.5∼1.0% 수준으로 26개월령까지 시험하였다. 시험구 배치는 C(무첨가), T1구(맥섬석 0.5% 첨가), T2구(한약제재 0.5% 첨가), T3구(맥섬석 0.5%+한약제재 0.5% 첨가)로 구분하였다. 증체량의 경우 비육전기때 T1구(226.2kg)가, 비육후기 때는 T3구(162.1kg)가 가장 높게 나타났다(p<0.05). 혈중 총콜레스테롤 함량은 T3구가 다른 처리구보다 유의적으로 높았다(p<0.05). 또한 도체중은 T3구(439.00kg) > T2(425.80kg) > T1(420.50kg) > Control(413.00kg) 순으로 낮아졌다. 배최장근 단면적은 T3구가 107.00㎠로 다른 처리구보다 높았고(p<0.05), 도체등급에서 육질등급은 T2구와 T3구가 공히 3.25로서 크게 높았다(p<0.05). 한편 등심육의 지방산 조성에서 oleic acid 함량이 48.55∼50.90%로서 가장 많은 비율을 나타내었고, 불포화지방산은 T3구(57.94%)가 다른 처리구보다 유의적으로 높게 나타났다(p<0.05). 따라서 거세한우에게 각각 맥섬석과 한방제재를 혼합하여 첨가급여하면 도체등급, 관능검사 및 불포화지방산의 개선에 도움이 될 것으로 판단된다.

목적 : 본 연구는 노인의 건강 증진 생활양식을 파악하고자 한글판 건강 증진 생활양식 프로파일(Korean Version of Health Enhancement Lifestyle Profile; K-HELP)을 개발하고 K-HELP의 신뢰도를 검 증하고자 하였다. 연구방법 : 작업치료사 Hwang에 의해 미국에서 개발된 Health Enhancement Lifestyle Profile (HELP)의 한글판 개발을 위해 번안과정 및 내용타당도 검증을 거치고 국내 상황에 맞게 문항을 수정하여 K-HELP를 완성하였다. 완성된 K-HELP의 신뢰도 검증을 위해 내적일치도 및 검사-재검사 신뢰도 검증을 실시하였다. 결과 : 완성된 한글 번역본 HELP을 보건의료전문가 9인에게 회부하여 검증받은 56문항에 대한 내용타당 도지수(Content Validity Index; CVI)는 .97로 높게 나타났다. 정상 노인 483명을 대상으로 한 신뢰도 검증 결과 전체 문항에 대한 내적일치도는 .82로 높은 수준으로 나타났고, 검사-재검사 신뢰도는 .99로 매우 높은 상관관계를 보였다(p<.01). 결론 : 본 연구는 K-HELP의 개발과 신뢰도 검증을 통해 K-HELP가 노인의 생활양식과 건강수준을 측 정하는데 신뢰도가 입증된 도구임을 증명하였다. 본 연구를 토대로 K-HELP가 노인 건강 증진에 대한 질병 예방 및 적절한 중재를 제공하기 위해 사용되는 유용한 평가도구로 활용될 수 있을 것이다.

본 동물실험은 STZ로 유도한 C57BL/6에게 5% sodium butyrate 를 급여했을 때 항당뇨 및 항염증 효과를 연구하고자 하였다. 본 연구에서 STZ로 당뇨를 유발한 마우스에게 5% sodium butyrate를 급여했을 때 체중과 식이섭취량에서는 크게 유의 적 차이가 없음을 확인하였다(p<0.05). STZ에 의한 당뇨 쥐는 인슐린의 분비가 감소되면서 당대사의 불균형을 초래하며 간 등이 비대해진다고 알려져 있으나, 본 연구에서는 간의 장 기 무게에서는 크게 실험군 간에 유의적인 차이가 없었다 (p<0.05). 또한 비장과 흉선의 무게는 0.5% sodium butyrate 첨가 식이군에서 유의적으로 낮아짐을 알 수 있었다(p<0.05). 당뇨병은 염증 상태로서 고혈당으로 인하여 monocyte에서는 여러 염증성 사이토카인이 분비가 활성화된다. TNF-α, IL-6 등은 염증성 사이토카인으로서 혈관염증의 중요한 마커로 인식되고 있고, 당뇨병 환자들은 이러한 염증성 사이토카인 이 높은 수준으로 활성화 된다. STZ 처리 시 마우스 혈청에서 의 염증성 사이토카인의 분비 및 발현이 증가되었으나, 5% sodium butyrate를 급여했을 때 염증성 사이토카인의 분비 및 발현이 저해됨을 확인할 수 있었다. 본 연구는 sodium butyrate 보충은 당뇨병이 유발된 동물모델에서 혈청지질 농도 및 혈 당 조절, 염증 상태를 개선에 다소간의 효과가 있는 것으로 나타났다. 이에 따라 당뇨병과 같은 만성적인 대사질환 개선 에 sodium butyrate가 효과적인 식이인자가 될 것으로 생각된 다. 그러나 앞으로 더 명확한 효능을 탐색하기 위해서 시료 첨가수준의 다각화 및 여러 가지 보완연구가 필요할 것으로 생각 된다.

가스폭발은 해양플랜트 산업에서 발생할 수 있는 치명적인 사고 중 하나이며, 탑사이드 플랫폼은 폭발압력에 따른 구조건전성을 확보해야만 한다. 따라서, 해양플랜트 분야에서는 이러한 폭발사고에 대비한 방폭설계에 관한 많은 연구가 수행되었지만, 여전히 추가적으로 세밀한 분석이 더 필요한 실정이다. 폭발 설계하중 계산과정에서 도출된 충격량은 CFD 해석결과로 계측된 폭발 압력 응답에서의 곡선 아래 면적의 절대 값에 의해 결정되어 진다. 하지만 가스폭발에서의 부압구간은 TNT 폭발이나 가스폭발과는 달리 상당부분 존재한다. 본 연구의 목표는 이러한 부압구간이 구조물의 거동에 미치는 영향에 대해서 분석하는 것이다. 따라서 방폭설계가 필수적으로 요구되어지는 FPSO 탑사이드의 방화벽을 폭발하중에 따른 구조응답을 분석하기 위한 대상물로 선정하였다. 폭발 하중-시간이력 데이터는 FLACS를 이용한 폭발 시뮬레이션 과정을 통해 획득하였으며, LS-DYNA는 비선형 과도 응답해석을 위해 사용되었다.

Matrix Metalloproteinases (MMP)-2 and -9 are participated in embryo development, implantation, remodeling of epithelial cell and ovulation. The objective of this study is to evaluate an impact of MMP2 and MMP9 on embryonic developmental competence as well as gene expression profiles of in vitro-produced bovine embryos. After in vitro fertilization, embryos of all groups were transferred into IVC-2 medium treated with MMP2 and MMP9 to check the optimum concentration on the basis of embryo development competence and cell numbers. The optimum concentrations for MMP2 and 9 were 1,200 ng/ml and 300 ng/ml. The blastocyst development competence was not different among 1,200 ng/ml of MMP2 vs. 300 ng/ml of MMP9 vs. combined MMP2 + 9 vs. control groups (41.46 ± 10.66 vs. 37.73 ± 8.92 vs. 45.11 ± 11.41% vs. 41.59 ± 11.88, respectively). Furthermore, the developmental competences to hatching and hatched blastocysts were not also different among the same groups (79.84 ± 12.63 vs. 83.3 ± 17.46 vs. 78.55 ± 14.48% vs. 72.02 ± 14.09). In addition, total cell number was significantly (p<0.05) greater in blastocyst treated with MMP9 300 ng/ml among all treatment groups. On the other hand, there was no significant difference of ICM vs. TE ratio in all groups. The expression of five out of six genes (i.e., MMP2, MMP9, IFNt, SSLP1 and HNRNPA2B1) was different among the groups. The expression of IFNt and HNRNPA2B1 genes was significantly greater in MMP9 (p<0.05), but there was no difference of MMP9 expression between MMP2 and MMP9 group (p>0.05). The normalized expression of MMP2 and SSLP1 was greater in MMP2 than other groups (p<0.05). In conclusion, MMPs treatment during IVC-2 medium was remarkably effected on blastocyst developmental competence and gene expression profiles that are related to embryo quality and implantation.

This study found antibacterial activity of DMfreeⓇ [green tea extract] on facultative bacteria by direct petri dish method and gene array of obligatory M. leprae infected mesenchymal stem cells (MSC). While DMfree showed DPPH radical scavenging effect and high contents of polyphenol, it did not inhibit growth of facultative bacteria such as E. coli and S. aureus on the petri dish. The result does not exclude a possible antibacterial effect of organic solvent extract of green tea rather than DMfree which comes from the water extract of green tea. Pre-treatment of DMfree appeared to have no effect on copy number of 14 genes compared with control MSC by real-time RT-PCR. However pre-treatment of DMfree on M. leprae infected MSC revealed a significant decrease of anti-inflammatory cytokine (IL-6), (P<0.038) and sharp downregulation of pro-inflammatory cytokine (IL-1). Enhanced expression of VEGFR-1 mRNA was noted in DMfree pretreated M. leprae infected MSC group (P<0.003). These results show that DMfree would stabilize M. leprae infected MSC from further inflammation by down-regulating anti-inflammatory cytokine (IL-6) and pro-inflammatory cytokine (IL-1β). This is the first report on DMfree inhibition of IL-6 and IL-1β expression in M. leprae infected MSC. Further experiments that detect protein levels of IL-1β and IL-6 may support the result of this gene array.