The aim of this study was to prepare rice straw silage using cabbage by-product and persimmon peel which are agricultural by-products produced during the same season in Korea. The treatments comprised a commercial corn silage as the control and four rice straw silages (P15-1, P15-2, P30-1, and P30-2) with different levels of persimmon peel supplementation (15%, 30%) and ensiled periods (1 or 2 months). The cabbage by-products were used to adjust silage moisture (approximately 65%). The quality of the experimental silages was evaluated based on organic acid content, palatability to three Holstein dairy cows, and manufacturing cost. In the corn silage, all chemical compositions, except total digestible nutrients and levels of lactic and butyric acids, were significantly (p<0.05) higher than those of the rice straw silages. However, considering the quality analysis using Flieg's score, the rice straw silage supplemented with 30% persimmon peel ensiled for 2 months (P30-2) was estimated as second grade to corn silage, and was relatively better in palatability to dairy cows than the other rice straw silages, which were considered third grade. The manufacturing cost of rice straw silages using cabbage by-product and persimmon peel compared to that of corn silage was reduced by 28%. Consequently, to prepare rice straw silage adjusted to 65% moisture using only cabbage by-products without inoculant, 30% of persimmon peel, 10% of ground corn, and 2% of molasses as a sugar source should be ensiled for at least 2 months.

본 연구는 사일리지 발효제 첨가가 볏짚 사일리지에서 곰팡이 독소 및 in situ 섬유소 소화율에 미치는 영향을 평가하고자 실시 하였다. 사일리지 발효제로 L. plantarum 단일제 및 L. plantarum 과 S. cerevisiae의 혼합제를 첨가하였을 때 사일리지의 발효 및 섬유소 분해에 영향을 주었을 뿐만 아니라 곰팡이독소 감소에도 영향을 주었다. 시험 시료에서 곰팡이독소 중 ochratoxin A 및 zearalenone만 발견되었다. Ochratoxin A 및 zearalenone는 대조구에서 각각 38.11±2.22 및 633.67±50.30 ug/kg 수준으로 발효제 첨가로 감소 경향이 나타났고, 혼합제에서만 각각 27.78±2.28 및 392.72± 25.04 ug/kg 수준으로 유의적 차이를 보였다(p<0.05). pH는 대조 구에 비하여 단일제 및 혼합제에서 낮았고(p<0.05), lactic acid는 대조구(8.18±0.93 mM)에 비하여 단일제(11.73±0.31 mM)가 높았고, 혼합제(16.01±0.88 mM)에서 가장 높은 수준을 나타내었다 (p<0.05). Acetic acid와 propionic acid는 발효제 첨가에 따라 유 의적으로 낮아짐을 발견하였다(p<0.05). 그리고 total VFA도 발 효제 첨가가 대조구에 비하여 낮았다(p<0.05). NDF 및 ADF의 반추위 in situ 분해율은 배양기간 동안 혼합제가 가장 높은 수준 의 분해율을 유지하였고, 다음으로 단일제가 높았으며, 대조구가 가장 낮은 수준을 유지하였다. 그리고 이들 NDF 및 ADF 분해율 은 각각 배양 12 및 24시간 이후 모든 시간대에서 실험구간 유의 차를 나타내었다(p<0.05). 이상의 연구 결과는 볏짚 사일리지 제조에서 사일리지 발효제 사용은 발효 및 섬유소 분해에 영향을 주었을 뿐만 아니라 곰팡 이독소 감소에도 영향을 주었다. 그리고 곰팡이독소 감소는 L. plantarum 단일제보다 L. plantarum과 S. cerevisiae 혼합제의 효능이 더 크게 나타났다. 따라서 사일리지 조제를 위한 발효제로 L. plantarum과 L. plantarum 첨가제의 사용은 사일리지의 품질 과 안정성을 더 증진할 것으로 사료 된다.

This study was conducted to research on the efficacy of chemical treatment as an effective method for reducing mycotoxin in rice straw silage. As a chemical treatment method, ammonia and sodium hydroxid were treated at 4% level of rice straws contaminated with mycotoxin, and the effects of silage storage on fungal toxin reduction, fermentation quality, and fiber digestion were evaluated. Aflatoxin B1, B2, G1, G2 and fumonisin B1, B2 as well as deoxynivalenol were not detected in all experimental groups, and ochratoxin A and zearalenone were detected. Ochratoxin A was detected lower in the chemical treatment than control (41.23 g / kg) (p<0.05). Zearalenone showed lower results in sodium hydroxide treatment (297.44 μg / kg) than control (600.33 μg / kg) and ammonia treatment (376.00 μg / kg) (p<0.05). The pH of rice straw silage was the lowest in ammonia treatment and the highest in sodium hydroxide treatment (p<0.05). The lactic acid contents of control and ammonia treatments were similar, but sodium hydroxide treatment was the lowest (p<0.05). Propionic acid was higher in the control than in the chemical treatments (p<0.05), and showed similar contents in the ammonia and sodium hydroxide treatment. Both the rumen microbial degradation rate of NDF and ADF showed the highest in sodium hydroxide treatment, followed by ammonia treatment, and the control showed the lowest level (p<0.05). Therefore, the results of this study are demonstrated to have a good effect on the treatment of ammonia and sodium hydroxide to reduce the mycotoxins and increase the rumen microbial degradation rate in the rice straw silage. Sodium hydroxide treatment was more effective in reducing mycotoxins and improving fiber degradation rate than ammonia treatment, but it is thought to have an inefficient effect on silage fermentation in rice straw silage.

The purpose of this study was to analyze the effectiveness of different storage periods of lactic acid bacteria (LAB)-fermented low moisture fresh rice straw silage. The low moisture fresh rice straw sample was inculcated with LAB and stored for different storage periods such as 45, 90, and 365 days, respectively. The low moisture fresh rice straw (LMFRS) silage inoculated with LAB exhibited reduction in pH throughout the fermentation as compared with the control (P<0.05). The lactic acid content was increased at the late fermentation period (90 and 365 days, respectively) in LAB inoculated LMFRS silage as compared with the control (P<0.05). In contrast, the acetic acid and butyric acid concentrations were slightly reduced in the LAB inoculated LMFRS silage sample at 90 and 365 days fermentation, respectively. Meanwhile, the non-inoculated LMFRS silage showed higher amounts of acetic acid and butyric acid at an extended fermentation with low bacterial population as compared with the LAB inoculated LMFRS silage. However, lactic acid concentration was slightly high in the non-inoculated LMFRS silage at early 45 days fermentation. Additionally, the nutrient profile such as crude protein (CP), acid detergent fibre (ADF), neutral detergent fibre (NDF), and total digestibility nutrients (TDN) were not significantly different in control and LAB inculcated samples during all fermentation. Though, the microbial population was greater in the LAB inoculated LMFRS silage as compared with the control. However, the massive population was noted in the LAB inoculated LMFRS silage during all fermentation. It indicates that the inoculated LAB is the main reason for increasing fermentation quality in the sample through pH reduction by organic acids production. Overall results suggest that the LAB inoculums are the effective strain that could be a suitable for LMFRS silage fermentation at prolonged days.

The objective of this study was to optimize analytical methods for ochratoxin A (OTA) and zearalenone (ZEA) in rice straw silage and winter forage crops using ultra-high performance liquid chromatography (UHPLC). Samples free of mycotoxins were spiked with 50 μg/kg, 250 μg/kg, or 500 μg/kg of OTA and 300 μg/kg, 1500 μg/kg, or 3000 μg/kg of ZEA. OTA and ZEA were extracted by acetonitrile and cleaned-up using an immunoaffinity column. They were then subjected to analysis with UHPLC equipped with a fluorescence detector. The correlation coefficients of calibration curves showed high linearity (R2 ≧ 0.9999 for OTA and R2≧0.9995 for ZEA). The limit of detection and quantification were 0.1 μg/kg and 0.3 μg/kg, respectively, for OTA and 5 μg/kg and 16.7 μg/kg, respectively, for ZEA. The recovery and relative standard deviation (RSD) of OTA were as follows: rice straw = 84.23~95.33%, 2.59~4.77%; Italian ryegrass = 79.02~95%, 0.86~5.83%; barley = 74.93~97%, 0.85~9.19%; rye = 77.99~ 96.67%, 0.33~6.26%. The recovery and RSD of ZEA were: rice straw = 109.6~114.22%, 0.67~7.15%; Italian ryegrass = 98.01~109.44%, 1.65~4.81%; barley = 98~113.53%, 0.25~5.85%; rye = 90.44~108.56%, 2.5~4.66%. They both satisfied the standards of European Commission criteria (EC 401-2006) for quantitative analysis. These results showed that the optimized methods could be used for mycotoxin analysis of forages.

본 연구는 충남 천안 국립축산과학원 식용벼 재배지에서 2012년부터 2013년까지 정상적으로 벼 재배가 이루어지고 수확이 완료된 볏짚을 이용하여 수행하였다. 젖산균 접종 유무에 따라 볏짚사일리지의 사료가치 변화는 나타나지 않았으나 클로렐라 처리구는 대조구에 비해 현저하게 조단백질 함량이 증가하였다(p<0.05). 그리고 클로렐라 농도의 증가에 따라 조단백질 함량이 증가하는 경향을 보였다(p<0.05). 모든 처리구에서 섬유소와 TDN 함량 및 in vitro 소화율은 차이를 나타내지 않았다. 그리고 젖산균 접종 유무에 따라 볏짚사일리지의 pH 및 유기산 함량 변화는 나타나지 않았으며 클로렐라 처리구도 무처리구에 비해 pH 변화는 나타나지 않았다. 그러나 클로렐라 처리구에서 젖산함량은 무처리구에 비해 유의적으로 증가하였으며 클로렐라 농도의 증가에 따라 젖산함량이 증가하는 경향을 보였다(p<0.05). 젖산균 단독접종구의 젖산균 수는 무처리구에 비해 현저하게 증가하였으며(p<0.05), 클로렐라 농도의 증가에 따라 증가하는 경향을 보였다(p<0.05). 그러나 효모와 곰팡이 수는 비슷한 수준을 나타냈다. 그러므로 볏짚사일리지의 사료가치와 품질은 클로렐라 첨가에 의해서 향상될 수 있다.

본 연구는 국내 생산 사료용 볏짚 곤포사일리지의 수확 후 저장기간 동안 생성된 곰팡이 오염도 및 곰팡이 종류를 조사하고 mycotoxin 노출 유해성을 구명하고자 실시하였다. 곰팡이 및 mycotoxin 오염을 조사하기 위하여 곰팡이 발생이 육안 식별이 안 되는 정상적인 것부터 오염이 심한 것까지 다양한 상태의 농가에서 급여하고 있는 33점의 볏짚 곤포사일리지 시료를 수거하여 조사하였다. 오염된 곰팡이는 흰색, 회색, 푸른색 및 검은 갈색 등 다양한 색

Recently, round bale silage is very popular preservation method in Korea. In autumn, many farmers usually make silage from fresh rice straw, but the quality of rice straw silage was not good. This study was conducted to find out the silage quality of fresh rice straw in the mid-western costal area in Korea. The average DM (dry matter) content was 40.5% and pH was 5.0. Forage quality of "A" and "D" farm silage was higher than that of others area. Silage quality was also higher in "A" and "D" farm silage of west costal area. The content of lactic acid of "A" and "D" farm silage was high and acetic and butyric acid was low. The result of this experiment indicated that the quality of fresh rice straw silage made by farmers was somewhat low. So we must guide farmers to making good quality of fresh rice straw silage.

본 연구는 효율적인 RS 조제기술을 규명할 목적으로 실시하였다. 연구는 RS의 저장부위에 따른 발효품질 및 사료성분의 변화에 대하여 검토하였는데, 곤포한 1개월 후에 3개의 RS(bale No. 1, bale No. II 및 bale No. III로부터 부위별 (상층부, 중앙부 및 하층부)로 채취하였다. 건물 함량은 상층부에서 bale No. I, bale No. II 및 bale No. III이 각각 64.5, 87.6 및 로 bale No. II가

본 실험에서는 볏짚에 사과박의 첨가비율을 달리하여 조제한 사일리지(볏짚:사과박 = 100:0, 80:20, 60:40, 40:60) 혹은 옥수수 사일리지에 건초 및 시판사료의 혼합 비율을 각각 40:40:20로 조제하여 한국 재래산양에게 급여하였고, 이들에 의한 사료섭취량, 소화율 및 질소 축적율 등을 조사하여 농산 가공 부산물의 사료화 가능성을 제시하고자 실시하였으며 그 결과를 요약하면 다음과 같다. 시험사료의 화학적 조성분은 조단백질 함량이 6.3∼

This experiment was carried out to determine the effect of storing method and wrapping frequency on the quality of round baled rice straw silage at experimental field of Grassland and Forage Crops Division, National Livestock Research Institute, RDA, Suwo

The quality of the rice straw silage added with apple pomace was investigated in this study and the amount of apple pomace added in different treatments were 0, 20, 40 and 60%, respectively. Crude protein contents (6.4-7.5%) of rice straw silage added wit

본 시험은 원형베일러를 이용한 생볏짚 사일리지의 조제 가능성을 구명하고 적정 첨가제를 선발하기 위하여 1997년 10월부터 1998년 6월까지 축산기술연구소 초지사료과에서 실시하였다. 첨가제는 생볏짚을 원형베일로 만들기전 무처리, 개미산, 당밀, 당밀+요소 및 젖산균제 등 5처리를 두고 난괴법 3반복으로 수행하였으며 사일리지 품질과 가축 섭취량 등을 조사하였다. 조단백질 함량은 당밀+요소 처리구에서 가장 높았으며 소화율은 당밀 및 젖산균 처리구에서 증가하였다. 건물률에 있어서는 개미산 처리구가 가장 높았고 대조구에서 가장 낮게 나타났으며 첨가제 처리간에는 차이가 없었다. 그러나 산도(pH)는 당밀 및 젖산균제 처리구에서 대조구보다 낮게 나타났다. 유기산 함량은 전체적으로 2%이하로 낮게 나타났으며, 젖산 함량은 당밀 및 젖산균제 처리구에서 유의적으로 증가하였고(P<0.05) 낙산 함량은 감소하였다. 암모니아태 질소의 함량은 당밀+요소 처리구에서 유의적으로 높았으며 당밀 및 젖산균제 처리구에서 10% 이하로 낮게 나타났다. 7일간의 가축 급여시험에서는 젖산균제를 처리한 생볏짚 원형베일 사일리지에서 두당 섭취량이 무처리 원형베일 사일리지와 건조볏짚에 비해 높게 나타났다. 생볏짚 원형베일 사일리지 생산비는 수거면적이 넓어짐에 따라 생산비가 크게 낮아졌다. 이상의 결과를 종합하여 볼 때 생볏짚을 원형베일 사일리지로 조제시 당밀 또는 젖산균제를 첨가할 때 가장 우수한 사일리지를 조제할 수 있었고 젖산균제 처리시 가축 섭취량도 증가되는 것으로 나타났다.

본 시험은 국내생산 주요 조사료인 볏짚, 옥수수사일레지, 목건초를 급여하면서 농후사료 수준을 조절하였을 때 반추생리 및 저작형태에 미치는 효과를 조사하기 위하여 농후사료 5개수준(0, 3, 6,9 12kg/일)에 젖소 15두를 공시하여 시험을 실시한 결과는 다음과 같다. 1. 건물섭취량은 볏짚, 옥수수사일레지, 목건초가 대사체중비 4.97, 5.47 그래고 5.60%를 섭취하였으며 농후사료 증가에 따른 조사료 건물섭취량 감소량은 볏짚 0.330, 옥수수

This study was conducted to evaluate effects of chopped and non-chopped rice straw on characteristics of silage-basedtotal mixed ration (TMR) according to the particle size, laceration, and in situ dry matter (DM) degradation. The threerice straw silages as low moisture unchopped (LMUC; 32.75% of moisture, unchopped), high moisture unchopped(HMUC; 42.05% of moisture, unchopped), and high moisture chopped (HMC; 44.71% of moisture, chopped to 30cmlengths) were tested. Samples were collected at every 5 minutes from 10 min of pre-mixing to 50 min. The percentageof >19mm in LMUC and HMC was decreased to 7.23% and 7.74% (p<0.05), respectively, and the percentage of 8mm>was increased to 5.81% and 5.24%, respectively. Furthermore, the laceration of forage by a TMR mixer showed that therewas little change in the reduction of 1.26% in HMC, but was reduced to 7.53% and 16.06% in LMUC and HMUC,respectively. The peNDF>8 was maintained 17~18.5% of the optimal requirement level for 15 to 45 min mixing in LMUCand for 30 to 50 min mixing in HMC, but it exceeded the level of peNDF>8 in the range of 21.49 to 22.53% for 50minmixing in HMUC. However, ruminal in situ DM degradation appeared as LMUC>HMUC>HMC. Therefore, theseresults suggest that the rice straw silage may be useful for high-yielding lactating cows, if it can be supplied with theadequate peNDF, and the limiting factor on DMI and DM degradation was reduced by crushing of the plant tissue, althoughthe rice straw silage was concerned to low quality forage.