Cordycepin is the principal bioactive compound produced by Cordyceps militaris and exhibits diverse pharmacological properties. However, cordycepin production is highly sensitive to cultivation conditions, leading to substantially variable production amounts and challenges in process optimization. An interpretable machine learning framework was established in this study to predict the cordycepin produced by C. militaris cultivated on Pinus densiflora sawdust. Three key cultivation parameters—input weight, growth weight, and particle size—were quantified using submerged mycelial culture. The cordycepin content was measured via high-performance liquid chromatography. Four predictive models (random forest, support vector machine, XGBoost, and artificial neural network) were optimized through a randomized hyperparameter search and evaluated using internal validation and Tropsha’s external quantitative structure-activity relationship criteria. The validation accuracy of XGBoost was the highest (root mean square error = 42.67 μg/mL), whereas the external performance of random forest was the most reliable (R² = 0.898). Shapley additive explanations revealed that input weight most strongly influenced cordycepin production, followed by growth weight and particle size, with distinct nonlinear and interaction-driven effects among the cultivation variables. Kernel density and dependence analyses confirmed the occurrence of multimodal production regimes associated with the substrate loading and particle size characteristics. Finally, the best-performing model was deployed through a streamlit-based graphical user interface, enabling the real-time prediction of cordycepin concentration with a 95% confidence interval. The results collectively demonstrate the utility of interpretable AI-driven modeling for unveiling complex biological responses, providing a practical decision-support tool for optimizing cordycepin production in fungal biotechnologies.

This study aimed to identify and evaluate potential alternative substrate materials for the cultivation of Lentinula edodes (shiitake mushrooms) to address the growing instability in the supply of oak sawdust. A selection of locally abundant resources, including citrus branches, buckwheat hulls, Cryptomeria japonica sawdust, Miscanthus, and Sasa quelpaertensis, was evaluated as potential substitutes for oak sawdust. The results indicated that Miscanthus is rich in iron and manganese, which are essential cofactors for lignocellulose-degrading enzymes, such as cellulases, laccases, and peroxidases. In vitro mycelial growth assays (column tests) indicated that Miscanthus facilitated the most rapid growth among the alternative substrates for the shiitake strains ‘Chamaram’, ‘L808, ’ and ‘Haleumang’.’ For the strains 'Sanjo 701' and 'Nongjingo’, both oak sawdust and Miscanthus demonstrated comparable mycelial growth rates. Further cultivation tests with the strain ‘L808’ revealed that Miscanthus, whether used independently or in combination with oak sawdust, resulted in cultivation periods, primordia formation, and yield characteristics comparable to those achieved with the oak sawdust medium. These findings suggest that Miscanthus has significant potential as an alternative substrate for shiitake cultivation. However, as the study employed small, laboratory-scale blocks weighing approximately 300 g, while farm-scale blocks typically weigh 1.3–1.5 kg, fruiting was limited to a first flush harvest. Consequently, additional validation using farm-scale blocks is necessary to assess their practical applicability.

This study was conducted to confirm whether domestic fruit tree pruning residues can replace sawdust, which is the main ingredient of mushroom medium. The five types of fruit tree pruning residues collected were from apples, pears, peaches, grapes, and citrus. The basic components of these residues were analyzed. The pH ranged from 5.2 to 7.0, the Total carbon(T-C) ranged from 46.0% to 47.2%, the Total nitrogen(T-N) ranged from 0.5% to 0.9%, and the moisture content ranged from 12.4% to 14.2%, which was identified as an appropriate range for growing mushrooms. In order to confirm the possibility of mycelial growth of oyster mushroom "Suhan", column tests were conducted based on the conventional medium(poplar sawdust 49.5: cotton seed hull 27.3: beet pulp 12.7: cotton seed meal 10.5, v/v). As a result of incubation at 25°C for 28 days, grape branches showed the fastest growth at 143 mm compared to the control, which showed 135 mm. The yield per bottle was comparable, with grape branches(134g/bottle) and control(139g/bottle). Additionally, the quality of the fruiting bodies was comparable across all Processed lots. It means grape branches can be used as alternative sawdust materal. In the future, it is expected that using by-products as substitutes for carbon sources and main ingredients will help reduce farm production costs and protect the environment.

A new oak mushroom cultivar ‘K815’ was bred from monokaryotic strains of ‘Jadam-2’ and ‘LE191732-118’. The optimal temperature for mycelial growth of ‘K815’ was 25oC, and the temperature for primordia formation and fruiting body development was 15~20oC. The fruiting body morphology is hemispherical and the fruiting form is sporadic. Total cultivation period of the new cultivar, from inoculation to its first harvest, was 133 days, which was 3 days longer than control cultivar ‘Jadam’ (129 days), with 32 days for spawn running, 89 days for browning, 4 days for primordia formation, and 8 days for fruiting body development. In the fruiting body, the thickness and diameter of pileus of the new cultivar were 32.2 mm and 58.3mm, respectively, and the length of stipe was 35.6 mm. Compared to the control cultivar ('Jadam', 'L808'), the pileus of the new cultivar was thicker and smaller and the length of stipe was shorter. The total number of fruiting bodies of ‘K815’ was 58.9, which was higher than the control varieties and total yield of 2 crop cycle of ‘K815’ was 580 g (per 3 kg substrate), which was similar to the control variety.

Cordycepin (3'-deoxyadenosine) is a nucleoside analog known for its diverse range of biological activities. This study investigated the effect of different types of sawdust on the production of the bioactive compound cordycepin. The results of the study showed that different types of wood sawdust affected the biosynthesis of cordycepin and a significant increase was observed when the conventional SDB medium was replaced with 1% NaOH treated pine sawdust. To optimize cordycepin production from Paecilomyces tenuipes in a medium containing 1% NaOH-pretreated pine sawdust, we employed Response Surface Methodology (RSM) in its Box-Behnken design (BBD) canonical form. The optimal conditions were determined as follows: a particle size of 109.5111-mesh (140 m) for 1% NaOH-pretreated pine sawdust, an input weight of 21.1679 g/L, and an incubation time of 73.8423 hours. According to our model, this combination is expected to yield a maximum cordycepin content of 896.1428 g/mL. Experimental validation of this prediction was performed using the suggested optimal conditions, resulting in an average cordycepin content of 922.6771 g/mL across three replicates, thus confirming the model's accuracy.

The effects of punching treatment on mycelial culture and fruiting body productivity were investigated in a new Lentinula edodes cultivar, “Jadam”, in sawdust medium for the stable production of oak mushroom. As the punching volume and number increased, the weight loss rate and color difference increased and the L value decreased. After spawn inoculation, the sawdust medium temperature and CO2 concentration reached their highest values at 33 and 19 days of incubation, respectively. The O2 concentration showed the lowest value on the 14th day of incubation, which was the opposite pattern to the CO2 concentration. As the punching volume and the number increased, the medium temperature and O2 concentration increased, and the CO2 concentration decreased. Higher punching volumes and numbers resulted in higher temperatures and lower CO2 concentrations. The best fruiting body yield was 5 × 70 mm - 30 (punching diameter × depth - number), and the total yield after three cycles was 644.7 g.

A new spore-less cultivar Lentinula edodes ‘Daedam’ was bred from monokaryotic strains of ‘LE15401-24’ and ‘LE192118-10’. The optimum temperature for mycelial growth of ‘Daedam’ on potato dextrose agar was 22~25oC. Total cultivation period of the new cultivar, from inoculation to its first harvest, was 134 days, similar to that of the control cultivar ‘Hwadam’. Total yield of ‘Daedam’ was 222g per 3kg substrate, and was lower than that of control cultivar(266.0g). The fruiting body of ‘Daedam’ had a thick and small pileus and a longer stem compare to control cultivar. As a result of a analyzing the productivity of ‘Daedam’ on the different substrate types, the biological efficiency was 26.7% in the 1.2kg cylindrical substrate(CS), which was higher than that of the 3kg rod-type substrate(RS). 'Daedam' had a similar yield compared to 'Hanacham' in first fruiting body production, but the cultivation period was 40 days shorter. Therefore, 'Daedam' can only harvest fruiting bodies once, it is thought that it can be used as spore-less oak mushroom cultivar for short-term cultivation instead of 'Hanacham' in mushroom farms.

베리류톱밥 발효기간중 톱밥내 온도변화와 발효기간에 따른 흰점박이꽃무지 유충의 발육특성을 조사한 결과 참나무톱밥에 비해 발효속도가 빠르고, 발효기간 50~70일이 유충 발육에 가장 적합하였다. 발효기간중 톱밥내 온도변화는 발효초기에 베리류톱밥이 참나무톱밥이 40℃이하로 경과한데 반하여 베리류톱밥은 60℃ 이상 고온으로 경과되면서 빠르게 발효가 되었고, 60일 이내에 안정화 되었다. 개체사육에서 발효기간별 흰 점박이꽃무지 유충의 총 발육기간은 베리류톱밥은 30일간 발효에서 104.8일로 가장 길고, 유의차가 인정되었으며, 50~90일 발효는 90일 정도로 차이가 없었다. 참나무톱밥도 베리류톱밥과 마찬가지 경향이나, 유충 발육기간이 30일정도 길었다. 유충 령기별 발육기간은 베리류톱밥과 참나무 톱밥에서 차이가 컸고, 유의차가 인정되었다. 톱밥 발효기간에 따른 유충 무게는 베리류톱밥과 참나무톱밥 모두 발효기간이 긴 90일 발효에서 가 장 가볍고, 발효기간 30~70일 발효에서는 차이가 없었다. 생존율은 개체사육에서는 95% 이상으로 발효기간에 따른 차이는 없었으나, 집단사육에 서는 개체사육보다 생존율이 낮고, 발효기간 90일에서 가장 낮았다. 집단사육에서 출하기준인 2.5 g 이상 유충 비율은 베리류톱밥 발효기간 30~70일까지는 85% 정도로 차이가 없었지만, 발효기간 90일에서는 70%로 낮고, 유의차가 인정되었다. 참나무톱밥도 마찬가지 경향이며, 베리 류톱밥에 비해 10%정도 낮았다. 또한, 베리류톱밥은 40~60일이면 유충의 무게가 2.5 g 이상이 되었고, 참나무 발효톱밥은 75~105일이 걸렸다.

흰점박이꽃무지(Protaetia brevitarsis seulensis) 유충을 사육상자(가로 543 × 세로 363 × 높이 188 mm) 안에서 뽕나무 발효톱밥 기반 사료 를 이용하여 집단으로 사육할 때(25°C, 16:8 L:D), 투입될 수 있는 갓 부화한 유충의 밀도를 검토하였다. 100-175마리 투입구는 90일후 생존율 이 80% 이상으로 사육밀도 사이에 차이가 없었다. 그러나 200마리 밀도에서는 생존율이 약 10% 이상 감소하였다. 100-150마리 밀도에서 유충 평균 무게는 처리 밀도 사이에 유의한 차이가 없었으나, 175마리와 200마리 밀도에서는 100마리 밀도에 비해 유충 무게가 유의하게 낮았다. 사 육밀도가 낮을수록 유충 무게가 더 빨리 증가하였다. 이상의 결과를 종합하여 100-150마리가 처음 투입밀도로 적절하다고 판단하였다. 두 번째 실험으로, 종령 유충을 저온에 보관하는 조건을 검토하였다. 종령 유충을 4개의 무게 집단(1.8-2.0, 2.0-2.3, 2.3-2.5, 2.5 g 이상)으로 구분하여 4, 8, 10°C에 보관하였을 때, 4°C에서는 70일 보관된 유충들은 무게에 관계없이 모두 사망하였다. 그러나 8°C에서는 70일 보관집단에서 약 80%의 유충이 생존하였다. 보관하는 유충의 무게가 무거울수록 생존율이 높았다. 4°C 보관에서는 무게가 2.3 g 이상인 유충을 50일까지 보관한 후 우화 한 성충들만 적은 수의 산란을 하였다. 8°C와 10°C 처리에서는 유충 무게에 관계 없이 모두 산란을 하였다. 이상의 결과로부터 무게가 2.3 g 이상 인 흰점박이꽃무지 종령 유충을 8°C에서 30-50일 범위에서 저장할 수 있다고 판단하였다.

This study was conducted to establish an appropriate period of use of sawdust spawn at low temperatures and a nutrient supplement medium for cultivation of Lentinula edodes ‘Hwadam’. Of the nutrient supplements, the total yield of rice bran (5%) + corn flour (5%) treatments were 673.3 g, which was higher than rice bran (551.6 g) and wheat bran (546.7 g) treatments, respectively. As shown by the growth of Lentinula edodes ‘hwadam' during to the sawdust spawn storage period (at 4oC), the period of spawn running, browning, fruiting body formation, and development was 27 d, 81 d, 5 d, and 11-13 d, respectively, regardless of the length of the storage period at 4 oC. After 3 months of storage of sawdust spawn, the number of fruiting bodies and yield decreased as the storage period increased. Therefore, the period of use of sawdust spawn (at 4 oC) for the stable production of fruiting bodies of Lentinula edodes ‘Hwadam’ was a maximum of 3 months.

뽕나무, 복분자, 블루베리의 전정 가지 대부분은 버려지고 있다. 따라서, 이들 버려지는 전정 가지를 활용하여 흰점박이꽃무지의 사육 가능 성을 검토하기 위하여 발육 및 산란 특성을 조사하였다. 베리류 발효톱밥을 먹인 흰점박이꽃무지 유충 발육 기간은 뽕나무 발효톱밥 157.3일, 복 분자 발효톱밥 130.3일, 블루베리 발효톱밥은 140일로 참나무 발효톱밥 169.3일에 비하여 짧아지고, 유충 무게가 증가하였으며, 생존율이 높았 다. 흰점박이꽃무지 유충 집단 사육시 2.5 g 이상인 유충 비율은 3종류의 발효톱밥 모두 6주에서 8주사이에 60% 이상인 반면 같은 기간에 참나무 발효톱밥은 10% 이내였고, 10주가 지나야 2.5 g 이상인 유충의 비율이 높아졌다. 흰점박이꽃무지의 평균 산란수는 80개 이상으로 평균 산란기 간은 9주정도 소요되었지만, 개체당 편차가 커서 유의차는 없었다. 3종류의 베리류 발효톱밥은 서로 혼합하여 사용해도 참나무 발효톱밥에 비해 유충 발육속도가 빠르고, 산란수는 차이가 없었다. 따라서, 흰점박이꽃무지를 사육하는데 있어서 베리류 발효톱밥이 참나무 발효톱밥에 비하여 효과적으로 추정된다.

국내 표고 품종 다양화로 버섯산업 경쟁력을 높이고자 ‘참아람’과 청흥버섯영농조합 보유균주인‘E140025’을 이용하여 교잡육종법을 통해 ‘청흥1호’를 육성하였다. 자실체 특성 결과 대조품종 보다 갓직경은 작고, 대길이는 길고, 대가 굵어 개체중이 무거웠다. 초발이기간은 ‘청흥1호’ 24일, ‘설백향’4일, ‘산조701호’ 8일로 발이까지 대조품종보다 20일정도 늦었다. 자실체 수량은 가장적게 나타났지만, 유효수량은 ‘청흥1호’ 6,498 g ‘설백향’ 4,290 g, ‘산조701호’ 5,022 g으로 가장 높았다. URPPrimer를 이용한 다형성검정을 통해 6개의 Primer에서 모본과 다른 PCR 밴드를 확인하였다. 이상의 결과, 재배기 간이 길지만 개체중과 유효수량이 우수한 무포자 신품종을 육성하였다.