여성은 연령과 사회경제적 위치에 따라 건강문제에 상당 한 차이가 있어 여성건강증진을 위해 지속적 노력을 기울여 야 한다. 숲은 현대인의 스트레스를 해소하고 면역력을 높 여주는 더할 나위 없는 자연환경이다. 숲 체험 경험이 있는 경우와 경험횟수가 많은 경우에 중년여성이 지각하는 삶의 만족도, 행복감, 희망수준이 유의하게 높은 것으로 나타났 다. 숲을 자주 이용할 수 있는 직장인들은 그렇지 못한 직장 인들에 비해 직무에 대한 스트레스가 낮은 것으로 나타났다. 이에 본 연구는 평소 숲 방문 빈도가 성인 여성의 스트레스 수준에 있어 어떤 차이를 가져오는지를 살펴보고자 했다. 20대 이상의 성인 여성 407명을 연구 대상으로 선정하여 숲 방문 빈도(숲 방문 빈도가 월 1회 이상인 144명은 “높음” 으로, 분기별 1회 이하인 263명은 “낮음”으로 분류)에 따라 구분한 두 집단 사이의 사회·심리적 스트레스(SF-PWI)와 스 트레스 반응(SRI)을 독립 t검증으로 비교하였다. 연구 결과 숲 방문 빈도에 따라 구분한 두 집단 사이의 사회·심리적 스트레스(SF-PWI)와 스트레스 반응(SRI)을 비교한 결과, 연령, 교육수준, 결혼 상태, 직업 등의 인구학 적 특성에서 통계적으로 유의한 차이를 발견하였다. 특히 50대 이상, 고학력의 전문․연구직, 교직, 400만원 이상이 각 각 숲 방문 빈도가 높게 나타났다. 우리나라 인구의 고학력 화와 고학력에 따른 경제활동 우위, 그로부터 얻어지는 경 제적 안정에서 원인을 찾을 수 있을 것이다. 고학력 전문직 에 종사하는 여성은 건강과 스트레스 감소를 위해 숲을 자 주 방문한다는 것으로 해석할 수 있다. 또한, 고졸, 100만원 미만의 저학력, 저소득의 무직·기타의 여성도 숲을 자주 방 문하는 것으로 나타났다. 이는 소득이 적을수록 비용이 상 대적으로 적게 드는 산책과 같은 활동을 선호하는 것으로 사료된다. 두 집단의 스트레스 수준을 분석한 결과 숲 방문을 통한 산림환경이용 빈도에 따른 차이를 확인할 수 있었다. 사회· 심리적 척도(SF-PWI), 스트레스 반응척도(SRI)에서 숲 방 문을 통해 산림환경 이용 빈도가 높은 집단이 사회·심리적 스트레스와 우울, 피로 등의 스트레스 반응이 적은 것으로 나타났다. 본 연구는 ‘숲 방문 빈도’만으로 숲 방문을 통한 효과를 측정했기 때문에 대상자들이 숲을 ‘어떻게 이용하는지’에 대해서는 확인할 수 없었다. 추후 연구에서는 스트레스 완 화에 더 효과적인 숲 이용방식과 산림치유 프로그램에 대한 후속연구가 필요하다. 본 연구 결과는 숲을 평소에 적극적으로 방문하는 것이 성인 여성들로 하여금 스트레스를 좀 더 적게 받게 하고, 피로도와 우울도를 낮추는데 도움이 됨을 시사한다. 본 연 구를 계기로 차후 성인 여성의 산림환경 이용방식에 대한 다각화된 조사와 함께 숲 방문 빈도가 비교적 활발한 직업 의 교사, 주부, 회사원 등의 직업에 대한 검증, 숲 방문 빈도 를 통한 산림환경이 일반 성인의 스트레스를 감소시키는데 어떻게 영향을 미치는지에 대한 연구가 이루어지기를 기대 한다.

Five heat shock protein 70 (hsp70) isoforms (hsc70-3, hsc70-4, hsc70-5, hsc70cb, hsp70Ab) of Apis mellifera were identified from Honeybee genome database. Specific primers for each isoform were designed for the quantitative realtime RT-PCR analysis, then analyzed transcript levels of the abdomen of adult workers (3-4 weeks old) in respond to heat shock and imidacloprid ingestion. Heat shock at 45°C for 1 h induced all 5 genes but highest in hsc70cb, hsp70Ab. Ingestion of imidacloprid pesticide did not change any hsp70 isoforms at 33°C but those bees also highly responsive to heat shock at 45°C. In addition, expression level of each hsp70 isoform was various and heat shock response of each isoform was tissue-specific. For example, hsc70-3 was highest in midgut, hsc70-4 was in hypopharyngeal gland, but hsp70Ab was in fat bodies. However, heat shock response of all 5 isoforms was the highest in the fat body than brain, hypopharyngeal gland, midgut, flight muscle and integument. Our results provide information for the understanding of complicated protective mechanism of honey bee against thermal stress.

The sweetpotato whitelfy Bemisia tabaci distribute worldwide and infests more than 500 species of plants. To determine nutritional stress of whiteflies at molecular level, we identified a full cDNA of glucose regulated protein 78 (grp78) which is known to be respond to nutritional restriction in vertebrate species. GRP78 of B. tabaci was highly conserved motifs of the HSP70 family and the C-terminal motif of KDEL characteristic of endoplasmic reticulum-specific HSPs. Real-time RT-PCR analysis showed that the grp78 level was not changed by thermal stress treatment from 4°C to 40°C for 1 h. However, the grp78 level was proportionally increased to the ingestion of a sucrose solution ranging in concentrations from 0% to 30% in a Parafilm feeding chamber. In addition, the grp78 levels were various by the ingestion of leaves of 10 different plants for 24 h; its level was higher with eggplant and pepper but lower with rice and apple. Our study suggests that the grp78 may have a role for cellular chaperones in relation to nutritional uptake of B. tabaci.

In order to reveal the aluminum (Al) stress tolerance mechanisms in alfalfa plant at low pH soil, a proteomic approach has been conducted. Alfalfa plants were exposed to Al stress for 5 days. The plant growth and total chlorophyll content are greatly affected by Al stress. The malondialdehyde (MDA) and H2O2 contents were increased in a low amount but free proline and soluble sugar contents, and the DPPH-radical scavenging activity were highly increased. These results indicate that antioxidant activity (DPPH activity) and osmoprotectants (proline and sugar) may involve in ROS (H2O2) homeostasis under Al stress. In proteomic analysis, over 500 protein spots were detected by 2-dimentional gel electrophoresis analysis. Total 17 Al stress-induced proteins were identified, of which 8 protein spots were up-regulated and 9 were down-regulated. The differential expression patterns of protein spots were selected and analyzed by the peptide mass fingerprinting (PMF) using MALDI-TOF MS analysis. Three protein spots corresponding to Rubisco were significantly down-regulated whereas peroxiredoxin and glutamine synthetase were up-regulated in response to Al stress. The different regulation patterns of identified proteins were involved in energy metabolism and antioxidant / ROS detoxification during Al stress in alfalfa. Taken together, these results provide new insight to understand the molecular mechanisms of alfalfa plant in terms of Al stress tolerance.

Firefighters receive extreme stress and suffer from PTSD in disaster. But we have not been paid attention to them. The purpose of this study is to analyze the problems of previous PTSD education programs in fire academy and to present the practical educational programs for firefighters to use in the disaster field. In the empirical analysis of this practical educational programs(EFT program), this program is more effective than existing theory-focused education training program.

Environmental risk assessment aims to estimate the impacts of various stressors on populations and communities in the environment. However, most of the exposure tests conducted under the laboratory level. This gap between the controlled condition of the experiments and the complexity of the field situation can lead to irrelevant estimation of stress effects. For this reason, dynamic model approach in ecology that including integrated mechanistic understanding has become important. The dynamic models at the individual level can be used to interpret the individual’s response to stress, extrapolate which response to untested conditions, and predict the impacts on the higher ecological level. The overall objective of this case study was to simulate the chronic toxicity of copper on Daphnia magna using dynamic energy budget theory with the improved toxicity module component. The model system was constructed and evaluated, using the PowersimⓇ software. The toxicity model system was integrated with toxic effects on allocation of reserve, structure, and maturity energy of D. magna into improved toxicity module. The model was calibrated and verified by actual data sets where obtained from a laboratory experiment including growth, maturity and survival measurement of D. magna during copper exposure. The simulation results showed that the response of D. magna under copper exposure was well estimated by model system.

This paper reviewed the relationship among job characteristics, psychological stress response, and accidents, and the mediating effect of psychological stress response between job characteristics and accidents. Based on the responses from 176 employees, the results of multiple regression analysis appeared as follow; 1) autonomy, importance and feedback effect negatively on psychological stress response. 2) psychological stress response effects positively on accident frequency. 3) psychological stress response mediates fully between autonomy and accident. 4) psychological stress response mediates partly between feedback and accident.

The green peach aphids, Myzus persicae (Sulzer), feed consistently and regularly throughout all stages of nymphs and adult. That is, honey dew production (HDP), which indicates a state of feeding, occurs at regular intervals within a given stage, and moving (MV), which interrupts feeding, tends to occur scarcely. We used these behaviors to test whether the sound stimuli acted as a stress in this species. We conducted the playback experiment in which acoustic stimuli with a combination of five frequency (100, 500, 1000, 5000, and 10000 Hz) and three intensity levels (66, 78, 90 dB SPL) were presented to all stages of aphids. Under all acoustic stimuli, HDP occurred less often, and MV occurred more often and longer. The results of two-way multivariate ANOVA showed that the stimulus frequency was a significant factor for variation in aphid behaviors in the playback experiment. However, there was no intensity effect on aphid’s behavior. Based on the electrical penetration graph experiment, aphid feeding was frequently stopped and lasted only for short durations under acoustic stimuli. The results of both experiments demonstrated that the acoustic stimuli were effective in inducing feeding suppression in this species. Thus, the acoustic stimuli may be a stressor in the green peach aphid. Furthermore, the behavioral bioassay employed in this study may be used to assess the effect of various stressors on aphids.

소음 스트레스로 인한 뱀장어(Anguilla japonica)의 영향을 파악하기 위하여 스트레스 지표로 사용되는 코티졸, 포도당, 알부민 및 glucocorticoid receptor(GCR) 유전자의 발현 양을 측정, 분석하여 노출되지 않은 대조구와 비교하였다. 그 결과, 알부민은 노출 1시간 후에 낮은 값을 보인 반면 코티졸과 포도당은 대조구에 비해 매우 큰 차이를 보이며 높게 나타났다. GCR 유전자의 조직 발현 결과 간, 아가미, 근육 및 소장에서 많이 발현하였다. 소음 노출에 따른 시간의 변화에서 간과 아가미 근육과 소장에서 발현이 감소하는 양상을 나타내었다. 실험결과 뱀장어의 glucocorticoid receptor 유전자의 발현변화가 소음 스트레스로 인한 영향을 파악하는데 유용한 지표가 될 수 있음을 확인하였다.

The purpose of this study was to examine the effects of stress response on safety behavior and to explore moderating effect of safety climate between stress response and safety behavior. 224 workers were asked to respond to the questionnaires that measured various demographic variables, stress response, safety climates and safety behaviors. A hierarchical regression was conducted to identify variables that had significant relationships with safety behavior and to examine moderating effect of safety climate between stress response and safety behavior. Results indicated that the depression response significantly predicted safety behavior. It was found that the safety climate was also a significant predictor for safety behavior. In addition, safety climate had a moderating effect on the relation between depression and anger responses and safety behavior.

Glutathione S-transferases (GSTs) are multifunctional enzymes that are mainlyinvolved in the xenobiotic metabolism and protection against oxidative damage. Most studies of GSTs in insects have been focused on their role in detoxifying exogenous compounds in particular insecticides. Here, we show the expression profiles of GSTs of the bumblebee Bombus ignitus in response to oxidative stress. We identified a sigma-class GST from B. ignitus (BiGSTS). The BiGSTSgene consists of 4 exons that encode 201 amino acids. Comparative analysis indicates that the predicted amino acid sequence of BiGSTS shares a high identity with the sigma-class GSTs of hymenopteran insects such as Apis mellifera (70% protein sequence identity) and Solenopsis invicta (59% protein sequence identity). Tissue distribution analyses showed the presence of BiGSTS in all tissues examined, including the fat body, midgut, muscle and epidermis. The oxidative stress responses analyzed by quantitative real-time PCR showed that under H2O2 overload, BiGSTS and BiGSTD (identified in our previous study) were upregulated in all tissues examined, including the fat body and midgut of B. ignitus worker bees. Under uniform conditions of H2O2 overload, the expression profile of GSTs and other antioxidant enzyme genes, such as phospholipid-hydroperoxide glutathione peroxidase (Bi-PHGPx) and peroxiredoxins (BiPrx1 and BiTPx1), showed that other antioxidant enzyme genes are acutely induced at 3 h after H2O2 exposure, whereas BiGSTS and BiGSTD are highly induced at 9 h after H2O2 exposure in the fat body of B. ignitus worker bees. These findings indicate that GSTs and other antioxidant enzyme genes in B. ignitusare differentially expressed in response to oxidative stress. Taken together, our findings indicate that BiGSTS and BiGSTD are oxidative stress-inducible antioxidant enzymes that may play a role in oxidative stress response.

저온 스트레스에 대한 내성을 지닌 신품종 톨 페스큐를 개발할 목적으로 CaMV35S 프로모터 하류에 NDP kinase 2 유전자가 항상적으로 발현하도록 제작한 벡터를 Agrobacterium법을 이용하여 톨 페스큐에 도입하였다. Hygromycin이 첨가된 선발배지에서 내성을 나타내며 재분화된 형질전환 식물체를 pot로 이식하여 기내순화 시킨 후, Southern blot 분석을 실시하여 본 결과, NDP kinase 2 유전자가 형질전환 식물체의 g

In order to obtain the fundamental data for the marine ranch, this paper was carried out to investigate cortisol variation in blood according to adapted time in the measurement of auditory threshold. The groups were adapted at the experimental tanks for 0.5, 1, 3 and 6 hours, respectively. At the results, cortisol concentration had a deep connection with adapted time. In particular, cortisol concentration in the experimental group of 1 hour was significantly increased, whereas others showed no difference comparing with the control group. When classical conditioning method with the sound coupled with a delayed electric shock was given after the adapted time of 1 hour and 6 hours, the rate of the conditioning completion for 6 hours was higher than that of 1 hour. Therefore, this suggests that the sufficient adapted time was required for the accurate auditory threshold.

수분 공급을 제한하여 수분 부족 스트레스를 처리한 상추식물에서 산화적 스트레스와 관련된 monodehydroascorbate reductase (MDHAR)의 활성도, 엽록소 함량, 과산화수소의 함량 등과의 상관관계를 조사한 결과, 생육배지의 수분의 함량이 감소함에 따라 식물체내 과산화수소의 생성량이 증가(R2=0.8851)하였으며, 수용성단백질 함량은 점차 감소(R2=0.9826)하는 경향을 나타내었다. 총 엽록소함량은 수분 부족 스트레스를 처리한 공시작물에서의 함량이 정상 생육시 보다 그 함량이 대체적으로 낮은 경향을 보였으며, 엽록소 a와 엽록소 b함량 변화도 총 엽록소의 함량변화와 비슷한 경향을 보였다. 그러나 총 엽록소에 대한 카로티노이드의 비율은 수분 부족 스트레스를 처리한 식물에서 정상생육 시 보다 더 높은 경향을 보였다. 수분 부족 스트레스가 진행됨에 따라 아스코브산의 함량은 정상 생육 시 보다 더 높은 경향을 보였으나 환원형인 디하이드로아스코브산의 함량은 수분 부족 스트레스를 처리한 초기에 정상생육 시 보다 더 낮은 경향을 보였다. MDHAR의 활성도는 사이토졸(cytosolic) 분획과 엽록체(chloroplastic) 분획에서 공히 크게 증가하였으며 MDHAR의 mRNA 전사 정도도 수분 부족 스트레스가 진행됨에 따라 크게 증가하였다. 수분함량이 감소함에 따라 MDHAR의 활성도가 크게 증가하였으며, 또한 과산화수소의 함량이 증가함에 따라서도 MDHAR의 활성도가 크게 증가(R2=0.9937과 0.8645)되어 수분 부족 스트레스로 나타나는 요인들과 MDHAR 사이에 밀접한 관련이 있음을 확인할 수 있었다.