This experiment was carried out to determine the effect of organic fertilizer and seed mixture on productivity, botanical composition and forage quality in middle areas(Geumsan, 250m sea level) pasture. Total six experimental pastures (orchardgrass, timothy and tall fescue based seed mixture and with or without organic fertilizer) were established in autumn, 2014 and evaluated productivity and agronomic characteristics from 2015 to 2016. Plant heigh was higher in orchardgrass based mixture and orchardgrass and tall fescue based mixture were higher in 2nd harvest. There was not found the significant difference among treatment(P<0.05) in dry matter (DM) content. Botanical composition in 1st harvest was higher weed portion in orchardgrass based mixture and legumes was disappeared at 3rd harvest in all treatments. Fresh and dry matter yield was higher in 2nd year than 1st year. In 1st year, orchardgrass based mixture with organic fertilizer plot was the highest(P<0.05) DM yield, but tall fescue based mixture with organic fertilizer was the highest in 2nd year. Content of crude protein (CP) was the highest in tall fescue based mixture and Timothy based mixture showed low in ADF (acid detergent fiber) and NDF (neutral detergent fiber) content. But the content if in virto dry matter digestibility was higher than others. In 2nd year, the nutritive value of 2nd and 4th harvested grasses were higher and average RFV (relative feed value) value was the highest at 4th harvest. According to this result, orchardgrass and tall fescue based mixture would be recommendable for farm who considering productivity and timothy based mixture would be recommendable for considering forage quality in middle areas of Korea.

The study was conducted to determine proper seeding rate and nitrogen fertilizer level for the effective seed production of Winter Rye (Secale cereale L.) with randomized block design with three replicates by combining both seeding rates (3, 5, and 7 kg/10a) and different nitrogen fertilizer application levels (0, 3, 6, and 9 kg/10a) in northern area of Gyeongbuk province from Oct. 7, 2013 to Jun. 15, 2014. Emergence and overwintering rates were not influenced but lodging degree was influenced by seeding rate and nitrogen fertilizer level. High lodging were observed at the seeding rates and nitrogen fertilizer levels of more than 5 kg/10a and 6 kg/10a, respectively (p < 0.01). Lodging less than 20% were observed at the seeding rate of 3 kg/10a regardless of the increase of nitrogen fertilizer level, furthermore, no lodging was shown though seeding rate was increased up to 7 kg/10a under the condition without nitrogen fertilizer. No. of leaves per plant, plant height and culm length were increased as affected by the increase of nitrogen fertilizer level rather than the increase of seeding rate. Spike length was shortened as affected by the increase of seeding rate while it was lengthened by the increase of nitrogen fertilizer level with no interaction effect between the two factors. No. of stems and spikes per m2 were influenced by the increase of nitrogen fertilizer level rather than by seeding rate with no interaction effect between the two factors. Grain yield was significantly increased as influenced by the increases of seeding rate (A) and nitrogen fertilizer level (B) with interaction effect (A × B), which resulted in the highest grain yield (310 kg/10a) at the seeding rate of 5 kg/10a and the nitrogen fertilizer level of 3 kg/ 10a. In conclusion, the proper seeding rate and fertilizer application level would be 5 kg/10a and 3 kg/ 10a, respectively to effectively produce the winter rye seeds in northern Gyeongbuk province.

국내육성 톨 페스큐 신품종 푸르미의 종자생산 기술개발과 채종짚의 사료가치 평가를 통해 국내에서 종자생산 체계를 확립하고자 국립축산과학원 초지사료과 시험포장에서 휴폭과 이른 봄 질소 시용량에 따른 종자생산성을 조사하였다. 휴폭 및 이른 봄 질소 시용량에 따른 주요 생육특성은 처리에 따른 차이는 나타나지 않으나 경제성을 고려한 국내육성 톨 페스큐 푸르미 품종의 종자생산성은 휴폭을 15 cm와 이른봄 질소시비량을 90 kg/ha로 처리하는 것이 2톤 이상 안정적인 종자생산을 할 수 있었다. 또한 휴폭이 좁을수록 이삭수 및 종자수량이 높게 나타났으며 이삭의 길이는 휴폭이 넓을수록 더 길었다. 채종종자의 첩립중은 약 2.2g, 발아율은 90% 내외로 나타났다. 휴폭 및 이른 봄 질소 시용량에 따른 채종짚의 생산성은 휴폭이 좁을수록 건물생산성이 높았으나 시비량간에는 일정한 경향이 없었다. 채종후 남은 1차 수확 채종짚은 평균 6,920 kg/ha 생산되었고, 채종 후 2번의 수확에서 건초는 평균적으로 8,134 kg/ha 생산되었다. 채종짚의 일반 조성분 함량은 양질의 건초보다는 다소 낮은 사료가치를 나타내었다.

phosphor powders were synthesized using a seed (average particle size: 5 ) by the polymer solution route. PVA solution was added to the sol precursors consisting of the seed powder and metal nitrate salts for homogeneous mixing in atomic scale. All dried precursor gels were calcined at and then heated at in atmosphere. The final powders were characterized by using XRD, SEM, PSA, PL and PKG test. All synthesized powders were crystallized to YAG phase without intermediate phases of YAM or YAP. The phosphor properties and morphologies of the synthesized powders were strongly dependent on the PVA content. Finally, the synthesized phosphor powder heated at , which is prepared from 12:1 PVA content and has an average particle size of 15 , showed similar phosphor properties to a commercial phosphor powder.

종자 건전성(seed health)은 종자 또는 식물체에 유익하지 못한 영향을 미칠 수 있는 생물체의 감염상태를 의미한다. 이들 생물체는 종자의 품질에 직접적인 피해를 줄 수 있으며,감염 종자는 식물체에 병을 유발하고 포장에서의 발병을 확산시킴으로써 대규모의 경제적 피해를 유발할 수 있다. 상업용종자의 해외증식 및 국제교역, 종자 유전자원의 해외분양 및 상호교환이 활발해짐에 따라 이러한 위험성이 더욱 커지고 있으며 더불어 종자 건전성 관리의 중요성 또한 증가하고 있다. 다양한 벼 종자 유전자원을 보존하고 연구용으로 분양하는 국제미작연구소(International Rice Research Institute, IRRI)는 유전자원의 건전성 확보를 위하여 종자건전성연구실(Seed health unit)을 두어 체계적인 관리시스템을 운영하고 있다. 필리핀 식물산업국 산하 식물검역소로부터 권한을 부여받아 필리핀 국내로 도입되거나 해외로 분양되는 유전자원에 대하여종자처리 및 건전성 모니터링, 종자 건전성 검사 및 데이터베이스 관리 업무를 수행하고 있다. 대상 병원균에 따라 육안검사, 수세현탁액 검사, 습지법 검사, 한천배지 검사 등을 적용하고 있으며, 감염종자는 온탕처리와 같은 물리적 처리와 살균제 또는 훈증제와 같은 화학적 처리를 수행한다. 국제미작연구소의 건전성 관리시스템을 우리나라 벼 종자 유전자원의보존·관리에 선별 적용하고, 더불어 종자소독방법과 저장 전관리체계를 확립함으로써 벼 유전자원의 품질을 제고할 수 있을 것이다. 또한 국제협력 및 공동연구 등 신뢰구축을 통해유용한 해외 유전자원을 확보하는 데 기여할 것으로 기대된다.

Growth characteristics and seed productivities of orchardgrass "Hwabsung 2 Ho" cultivated for seed production were examined in the NLRI from 2000 to 2002. The experimental design was a split-plot arrangement with three replications. The main-plots were th

이탈리안 라이그라스의 생육특성 및 종자 생산성을 조사하기 위하여 축산기술연구소 초지 사료과 시험포장에서 2000년부터 2001년까지 실시하였다. 시험구 배치는 휴폭(15, 30, 45cm) 및 이른 봄 질소시용량(50, 75, 100kg/㏊)을 조합한 분할 구배치 3반복으로 설계되었다. 생육특성 중에서 도복저항성은 휴폭이 45cm로 넓고 이른 봄 질소시용량이 50kg/㏊로 적을 때 높게 나타났고, 줄기의 수는 휴폭이 45cm 보다는 15 또는 30cm에

식물생장 조절제 Uniconazole-P의 처리가 초지조건에서 목초의 생육억제와 종자생산에 미치는 영향을 검토하였다. 초종은 화본과 목초 2초종(orchardgrass와 timothy)과 두과목초 2초종(red clover와 alfalfa)을 공시하였고, Uniconazole-P의 처리농도는 대조구, 20ppm구, 40ppm구의 3처리로 하여, 화본과 목초는 유수분화기에, 두과목초는 개화시기(開花始期)에서 엽면산포하였다. 본 실험에서 얻어진 결과를 요약하면 다음과 같다. 1. Uniconazole-P의 처리효과는 처리농도, 초종 및 생육단계에 따라서 차이가 인정되었다. 2. Uniconazole-P의 처리는 출수초기의 화본과 목초에서 초장과 간장이 단축되었고, 수중과 엽록소 함량을 증가시켰다. 3. Uniconazole-P의 처리는 종자 결실기의 orchardgrass에서 수수, 1경중, 수중, 1수중 및 종자중을 증가시켰고, timothy에서는 초장의 단축과 수수, 1수중, 수중, 종자중 및 수확지수를 증가시켰다. 4. Uniconazole-P의 처리는 개화초기의 red clover와 alfalfa 2초종 모두 초장과 총절간장을 단축시켰지만, 총분지장과 엽록소 함량을 증가시켰다. 특히 red clover에서는 화수와 화중의 증가가 현저하였다. 5. Uniconazole-P의 처리는 종자 결실기의 red clover에서 초장을 단축시켰고, 화중과 종자중을 증가시켰으며, alfalfa에서는 초장을 단축시키고 화중, 종자중 및 수확지수를 증가시켰다. 6. Uniconazole-P의 처리에 의한 화본과 목초의 종자 생산과정은 1) 출수초기에서 초장과 간장의 단축, 2) 출수초기와 종자 결실기에서 수수와 l수중의 증가, 3) 종자 결실기에서 단위면적당 수중과 종자중의 증가 및 수확지수의 증가로 설명할 수 있다. 7. Uniconazole-P의 처리에 의한 두과목초의 종자 생산과정은 1) 개화초기에서 초장과 총절간장의 단축과 분지수와 총분지장의 증가, 2) 개화초기와 종자 결실기에서 화수와 화중의 증가, 3) 종자 결실 기에서 종자중과 수확지수의 증가로 설명할 수 있다.

The antibacterial and antifungal effect of grapefruit seed extract(GFSE) was investigated for its purpose of application to a diverse spectrum of field as sanitizers, disinfectants and preservatives. GFSE showed comparatively high content of such flavoniods as naringin and hesperidin and ascorbic acid. GFSE containing a low level of organic acids is a heavy viscous and water-soluble liquid. As a result of the antimicrobial test of GFSE, Bacillus subtilis and Aspergillus oryzae did not survive at detectable levels when treated with more than 100 ppm of GFSE. The minimum inhibitory concentrations of GFSE for a wide variety of pathogenic and putrefactive bacteria, fungi and yeasts were 100 ppm and 250 ppm, respectively. In the comparable electron micrograph of microbial cells treated with GFSE or not, we could conclude that GFSE destroy microorganisms by disrupting the functions of the cell wall membrane and microbial spores.

본(本) 시험(試驗)은 Tall fescue(Festuca arundinarea s. 의 Alta품종(品種)에 있어서 채종재배(採種栽培)를 위(爲)한 질소비료(窒素肥料)의 시비수준(施肥水準) 및 분시방법(分施方法)을 구명(究明)코자 3 시비수준(施肥水準)(120, 180, 240kg/ha)과 4 분시방법(分施方法)((1)봄 : 100%%, (2) 가을, 봄 : 각각 50%, (3)봄, 출수전 : 각각 50%, (4) 가을 : 50%, 봄, 출수전 : 각

The vegetation-based spraying method has been used as a revegetation measure for protecting slopes. However, after the method is applied to the fields, the collapse due to deteriorated bond performance of artificial slopes and defective vegetation growth bases prevent it from displaying its proper function. Therefore, research and application technologies for revegetation measures that can achieve both the reinforcement of slopes and the ecological restoration at the same time are necessary. Accordingly, in this study, fundamental research was conducted regarding the bond properties of slopes, according to the addition of additives based on bonding materials for improving bond performance.

Field emergence of Azuki bean is poor due to hard seed coat as compared to other legumes. In this study, an attempt was made to develop prediction method with regression analysis based on various seed vigor tests in laboratory for field emergence of azuki bean. Azuki bean seeds artificially aged to provide various levels of seed quality were evaluated by the standard germination test (SGT), cold germination test (CT), cool germination test (CGT), complex stressing vigor test (CSVT), tetrazolium(TZ) vigor test and electroconductivity test. The SGT was suitable for predicting the field emergence in the unaged high vigor seeds. The abnormal seedling percentage and shoot length in the CGT were highly correlated with field emergence of moderate vigor seeds artificially aged for 2 days. Electroconductivity, seed viability in the CSVT, and vigor and predicted germinability in the tetrazolium vigor test were also useful for predicting field emergence. Percent of ungerminated seed in the CSVT was correlated with field emergence in the low vigor seeds artificially aged for 4 days. In a stepwise multiple regression analysis, seed viability in the SGT, normal seedling percentage and dry matter weight in the CGT accounted for 86.9% of the predicted value of field emergence in azuki bean.

We improved the separation of the basic proteins from the soybean cotyledon, Glycine max L. Merr. by searching N-terminal sequences data in proteins isolated by two-dimensional electrophoresis (2-DE). After removed Hexane, proteins were extracted from cotyledon with a urea/Triton/2-mercaptoetanol solution. Using this method, the highly reproducible isoelectric focusing (IEF) can formed with polyacrylamide gels with pH 4.0-9.8. The IEF tube gels were used as the first dimension, and proteins were visualized by second-dimensional gel electrophoresis, and identify a number of soybean cotyledon proteins using mass spectrometry in the proteome analysis. These instruments of 2-DE and IEF tube gels were used 27 cm and investigate under various conditions. The total number of spots and features was obtained by PDQuest software (Bio-Rad). In this experiments performed, the IEF tube gels and instruments afforded good reproducibility in the number of PDQuest-detected spots from gel to gel while IPG offered better reproducibility in the total number of manually detected spots from gel to gel. In conclusion, we have separated of the basic 13 proteins in soybean. The glycinin subunit separations are also considered to play important roles in soybean breeding and biochemical characterization. The improved technique will be useful to dissect the genetic control of glycinin expression in soybean.

Kjeldahl method used in many materials from various plant parts to determine protein contents, is laborious and time-consuming and utilizes hazardous chemicals. Near-infrared (NIR) reflectance spectroscopy, a rapid and environmentally benign technique, was investigated as a potential method for the prediction of protein content. Near-infrared reflectance spectra(1100-2400 nm) of coarse cereal grains(n=100 for each germplasm) were obtained using a dispersive spectrometer as both of grain itself and flour ground, and total protein contents determined according to Kjeldahl method. Using multivariate analysis, a modified partial least-squares model was developed for prediction of protein contents. The model had a multiple coefficient of determination of 0.99, 0.99, 0.99, 0.96 and 0.99 for foxtail millet, sorghum, millet, adzuki bean and mung bean germplasm, respectively. The model was tested with independent validation samples (n=10 for each germplasm). All samples were predicted with the coefficient of determination of 0.99, 0.99, 0.99, 0.91 and 0.99 for foxtail millet, sorghum, millet, adzuki bean and mung bean germplasm, respectively. The results indicate that NIR reflectance spectroscopy is an accurate and efficient tool for determining protein content of diverse coarse cereal germplasm for nutrition labeling of nutritional value. On the other hands appropriate condition of cereal material to predict protein using NIR was flour condition of grains.

The objective of this study was to rapidly evaluate fatty acids in a collection of foxtail millet (Setaria italica (L.) P. Beauv) of different origins so that this information could be disseminated to breeders to advance germplasm use and breeding. To develop the calibration equations for rapid and nondestructive evaluation of fatty acid content, near-infrared reflectance spectroscopy (NIRs) spectra (1104-2494 nm) of samples ground into flour (n=100) were obtained using a dispersive spectrometer. A modified partial least-squares model was developed to predict each component. For foxtail millet germplasm, our models returned coefficients of determination (R2) of 0.91, 0.89, 0.98 and 0.98 for strearic acid, oleic acid, linoleic acid, and total fatty acids, respectively. The prediction of the external validation set (n=10) showed significant correlation between references values and NIRs values (r2=0.97, 0.91, 0.99 for oleic, linoleic, and total fatty acids, respectively). Standard deviation/standard error of cross-validation (SD/SECV) values were greater than 3 (3.11, 5.45, and 7.50 for oleic, linoleic, and total fatty acids, respectively). These results indicate that these NIRs equations are functional for the mass screening and rapid quantification of the oleic, linolenic, and total fatty acids characterizing foxtail millet germplasm. Among the samples, IT153491 showed an especially high content of fatty acids (84.06 mg g-1), whereas IT188096 had a very low content (29.92 mg g-1).

This study was conducted in an effort to evaluate the effect of two gibberellin biosynthetic inhitiors, mepiquat chloride (MC 200, 400, 600 ppm) and trinexapac-ethyl ethyl (TNE 100, 200, 300 ppm) on flax (Linum usitatissimum L.) seed oil composition, growth characteristics and seed yield when applied at vegetative stage. A foliar spray of 600 ppm MC was found to be most effective, resulting in decreased plant height, and an increased seed yield (1,963 kg ha-1) with highest oil content (37.2 %). Ripened seed rate ranged 90.2 –91.9% in MC treated flax and 93.7- 94.1% in TE treated flax, respectively. MC treatment increased seed yield 10.9 – 21.6% and TNE treatment also increased 7.9 –11.8% compared to the control. The significant increase of flax seed yield in MC treatment was observed 600 ppm between these concentrations. Two gibberellin biosynthetic inhibitors, MC and TNE were 1.6 – 5.5% more ripened compared to the control. An increased unsaturated fatty acid was observed when higher concentration was applied to flax plant. Endogenous GA4 content was always higher than GA1 content in both MC and TE treatment.

barley grain and malt is highly related to beer quality, especially hordein is known to be a more significant factor in malting process than albumin. In this study, we proposed selection criteria for high quality malting barley with aid of grain and malt quality parameter scores and storage protein subunit profile informations. Albumin and hordein were extracted and denatured protein subunits were evaluated with malt and grain quality parameters. Total 13 local adaptability test (LAT) lines were planted in four locations (Naju, Iksan, Jeju, and Jinju) and evaluated for malt and beer making qualities. Seventeen germplasms (world collections for high or low seed storage protein content) were also evaluated for biochemical genetic marker. Denatured seed storage protein subunits of albumin and hordein of all tested lines and germplasms were evaluated using 12% 1D SDS-PAGE. Scored data of protein subunit's presence or absence was applied to Agglomerative Hierarchical Clustering (AHC) for statistical analysis. Subunits fractionated within specific molecular weight ranges (97.4-31.0, 66.2-31.0, and 45.0-31.0 kDa) were highly correlated with agricultural characteristics. Several LAT lines showing good performance in agricultural characteristics were clustered in dendrogram constructed by biochemical-genetic assay using XLSTAT. Specific band pattern showed in good performance LAT lines were also observed in some germplasms of world collection having low protein contents which are known to have superior quality in malting. The results would provide selection criteria for high quality malting barley in the malting barley breeding program.

천연물로부터 새로운 기능성 화장품 소재를 개발하기 위하여 국내외 천연물을 대상으로 항산화 활성을 평가한 결과, 가시연꽃의 종자 추출물이 우수한 활성을 나타내는 것을 발견하였다. 가시연꽃 종자의 추출물로부터 유효 성분을 분리하기 위하여 각종 컬럼 크로마토그래피 및 HPLC 등의 기법을 실시하여 순수하게 정제된 화합물 5종을 획득하였다. 각종 NMR 및 MS 스펙트럼 등을 분석하여 이들의 화학구조를 규명한 결과 fucosterol (1), 3-(4-hydroxy-3-me-thoxybenzyl)-4-[(7'R),5'-dihydroxy-3'-methoxybenzyl]tetrahydrofuran (2), resorcinol (3), pyrogallol (4) and 4-O- methyl gallic acid (5)로 동정하였다. 이들 화합물의 화장품 원료로서의 특징을 조사하고자 각 화합물들을 대상으로 항산화 활성, 엘라스타아제 저해활성, 미백활성 등을 평가하였다. 이들 화합물의 자유라디칼소거활성은 SC50 값이 17.0 ∼ 100.2 µM이었고, 특히 화합물 4 및 5는 양성대조구인 ferulic acid보다 6배 이상 강력한 활성을 나타내었다. 또한 엘라스타아제 저해활성에 대한 IC50 값은 18.8 ∼ 78.2 µM로써 특히 화합물 4는 양성대조구인 EGCG와 비슷한 활성을 나타내었으며, B16F10 멜라노마 세포를 이용한 멜라닌 생성 저해활성에 대해 화합물 3은 양성대조구인 arbutin보다 약 2배 이상 강력한 미백활성을 나타내었다. 따라서 가시연꽃 종자 추출물은 강력한 항산화활성, 엘라스타아제 저해활성 및 미백활성을 나타내는 유효성분들을 다량 함유하므로, 복합 기능성을 나타내는 화장품 원료로 사용할 수 있을 것으로 사료된다.

본 연구는 게르마늄 함유 녹두 생산을 위해 게르마늄의 효과적인 처리방법과 이행특성 등을 구명하고자 수행하였다. 1. 액상 게르마늄(7 mg/l) 개화기 엽면살포구 1, 2차 수확 종실의 게르마늄 함량은 각각 38.7, 14.1ug/kg 로 입상 게르마늄(7 mg/kg)을 기비와 함께 시용하는 것보다 각각 2.5, 2.3배 많다. 2. 게르마늄 3.5, 7, 14, 28 mg/l 를 개화기 엽면살포구 1, 2차 수확 종실의 게르마늄 함량은 각각 14.9~77.8ug/kg,6.9~26.7ug/kg 로 엽면살포 농도에 비례하여 많았으나 1차 수확 종실이 2차 수확 종실에 비해 2.2~4.1 배 많다. 반면에 무처리구 1, 2차 수확 종실의 게르마늄 함량은 각각 1.9, 3.2ug/kg 이었다. 3. 게르마늄 7 mg/l를 2회나 3회 엽면살포시 1~3 차 수확 종실의 게르마늄 함량은 20ug/kg 이상으로 일정 수준의 게르마늄 축적이 가능하였다. 4. 게르마늄 96ug/kg 이 함유된 녹두 종실에서 자엽의 게르마늄 함량은 138ug/kg 으로 종피보다 단위 중량당 79% 많았다. 5. 본 연구에 이용된 게르마늄의 제형, 엽면살포 농도 및 횟수에 따른 녹두 생육과 수량은 유의차가 없다.