이 연구를 통해 우리나라에서 복원 중인 멸종위기종인 반달가슴곰(Ursus thibetanus ussuricus)의 식별을 위한 새로운 분자 marker 체계를 개발하였다. 전장 유전체 서열 정보를 사용하여, 20개의 새로운 미세부수체(Microsatellite, MS) marker가 다중 중합효소연쇄반응(PCR)을 기반으로 하는 유전자형 분석을 통해 개발되었다. 개발된 MS marker 20종의 대립유전자 수는 3개에서 10개였으며, 평균 대립유전자 수는 6.05개였다. 관측 이형접합도(Hobs), 기대 이형접합도(Hexp), 다형정보량(PIC)의 평균은 각각 0.683, 0.715, 0.658로 나타났다. Fis 값은 –0.385에서 0.438의 범위에 있었고, 평균은 0.052였다. 무작위 교배(PIrandom), 반형매 교배(PIhalf-sib), 전형매 교배(PIfull-sib)에서의 동일개체출현율은 각각 1.58×10-18, 2.79×10-14, 4.75×10-8를 나타내었다. 이 연구의 결과는 새로운 MS marker 체계가 높은 유전적 다양성과 낮은 PI 수치를 가지고 있어, 야생에서 출현하고 있는 반달가슴곰들을 식별하거나 새끼 곰의 부모 추적에 유용하게 사용될 것으로 생각된다. 이 분자 marker 체계는 한국에서 복원 중인 반달가슴곰 개체군의 관리와 유전적 다양성 증진에 기여할 수 있을 것으로 사료된다.

괭이갈매기는 국내에서 서식하는 대표적인 바닷새로, 서해와 남해뿐만 아니라 동해까지 집단 번식지가 존재하고 있다. 하지만 현재 괭이갈매기에 대한 연구의 대부분이 서해와 남해에서 번식하는 개체에 집중되고 있어, 동해 지역에 대한 연구가 상대적으로 부족한 상황이다. 이에 본 연구에서는 동해 울릉도에서 번식하는 괭이갈매기 4개체를 대상으로 2021년과 2022년 5월에 야생동물 위치추적기(WT-300)를 부착하여 이동경로와 행동권 분석을 실시하였다. 괭이갈매기는 번식기간에 울릉도 주변 또는 강릉에서 울진에 이르는 동해 연안까지 활동범위로 삼았고, 번식기 이후에는 일본의 중남부 지역으로 이동하여 서식하는 것으로 나타났다. 국내에서 번식기간 MCP 분석에서 행동권 평균면적은 9,476± 10,066㎢로 확인되었고, KDE 분석에서는 95%일 때 3,574±3,378㎢, 50%일 때 289±200㎢로 나타났다. 번식기간 이후 일본에서의 MCP 행동권 평균면적은 1,899±3,127㎢이었고, KDE 95%는 2,844±4,787㎢, KDE 50%는 385±635㎢ 의 행동권을 보여주었다. 본 연구는 동해와 일본 연안을 이동하며 서식하는 괭이갈매기의 행동권을 제공함으로써, 괭이갈매기의 보전과 관리를 위한 기초자료로 사용될 수 있을 것으로 생각된다.

흑비둘기는 상록수림이 분포하는 해안가 및 도서 등 동아시아-태평양 섬에만 분포하는 희귀한 종으로 생물학적 보존 가치가 높아 국내에서 천연기념물 및 멸종위기야생생물Ⅱ급으로 지정되어 있다. 본 연구는 울릉도에서 서식하는 흑비둘기 3개체를 대상으로 2021년과 2022년 6월에 야생동물 위치추적기(WT-300)를 부착하여 이동경로 및 행동권을 분석하였다. MCP(최소볼록다각형법) 분석에서 행동권 평균 면적은 9.6㎢로 확인되었고, KDE(커널밀도추정법) 는 95%일 때 6.6㎢, 50%일 때 1.8㎢로 나타났다. 개체별 MCP는 각각 BW1 0.4㎢, BW2 27.4㎢, BW3 1.0㎢으로 확인되었며, KDE 95%일 때 BW1 0.2㎢, BW2 19.4㎢, BW3 0.3㎢, KDE 50%일 때 BW1 0.02㎢, BW2 5.4㎢, BW3 0.03㎢로 나타났다. BW2의 행동권이 다른 개체들 보다 넓게 분석되었으며, 이러한 차이는 한정된 지역에 먹이 경쟁 등 서식환경에 따른 차이로 추정된다.

This paper presents a finite-difference method (FDM)-based heat-transfer model for predicting black-ice formation on asphalt pavements and establishes decision criteria using only meteorological data. Black ice is a major cause of winter road accidents and forms under specific surface temperature and moisture conditions; however, its accurate prediction remains challenging owing to dynamic environmental interactions. The FDM incorporates thermodynamic properties, initial pavement-temperature profiles, and surface heat-transfer mechanisms, i.e., radiation, convection, and conduction. Sensitivity analysis shows the necessity of a 28-d stabilization period for reliable winter predictions. Black-ice prediction logic evaluates the surface conditions, relative humidity, wind speed, and latent-heat accumulation to assess phase changes. Field data from Nonsancheon Bridge were used for validation, where a maximum prediction accuracy of 64% is indicated in specific cases despite the overestimation of surface temperatures compared with sensor measurements. These findings highlight the challenges posed by wet surface conditions and prolonged latent-heat retention, which extend the predicted freezing duration. This study provides a theoretically grounded methodology for predicting black ice on various road structures without necessitating additional measurements. Future studies shall focus on enhancing the model by integrating vehicle-induced heat effects, solar radiation, and improved weather-prediction data while comparing the FDM with machine-learning approaches for performance optimization. The results of this study offer a foundation for developing efficient road-safety measures during winter.

This study developed conductive inks composed of carbon black (CB) and silver nanowires (Ag NWs) for cost-effective screen-printing on fabrics. The Ag NW density within the CB matrix was precisely controlled, achieving tunable electrical conductivity with minimal Ag NW usage. The resulting inks were successfully patterned into shapes such as square grids and circles on textile surfaces, demonstrating excellent conductivity and fidelity. Adding 19.9 wt% Ag NWs reduced sheet resistance by ~92% compared to CB-only inks, highlighting the effectiveness and potential of this hybrid approach for cost-effective, high-performance textile-based electronics. The one-dimensional morphology of Ag NWs facilitated the formation of conductive percolation networks, creating efficient electron pathways within the CB matrix even at low loadings. This work advances the field of CB-based conductive inks and provides a scalable and practical method for producing functional, patterned electronic textiles.

본 연구는 아메리카동애등에(Hermetia illucens)의 대량 사육을 위하여 남은음식물 사료의 최적 수분 함량과 적정 사육 밀도를 조사하였다. 실 험에서는 500 g의 습식 사료의 수분 함량을 각각 30%, 50%, 70%, 90%로 조정한 후, 3,500마리의 3령 유충을 7일 동안 사육하였다. 그 결과, 90% 수분 함량에서 평균 유충 무게는 7.19 g으로 측정되었고, 70% 수분 함량에서 가장 높은 무게인 10.54 g이 나타났다. 반면, 50%와 30% 수분 함량에 서는 유충 생육이 저조한 결과가 나타났다. 적절 사육 밀도를 조사를 위해 100,000, 150,000, 200,000, 250,000 마리의 3령 유충을 70% 수분함량 의 100 kg 습식 사료에 7일 동안 처리하였다. 그 결과, 250,000 마리에서 총 유충 생산량이 22.79 kg으로 가장 높게 나타났으나, 150,000, 200,000, 250,000 마리 밀도 간에는 유의미한 차이가 없었다. 100마리당 평균 무게는 100,000 마리에서 16.90 g, 150,000 마리에서 15.20 g, 200,000 마리 에서 11.30 g, 250,000 마리에서 9.50 g으로 확인되었으며, 100,000 마리와 150,000 마리 밀도 간에는 유의미한 차이가 없었다. 결론적으로, 남은 음식물 사료인 습식사료 100 kg를 급여할 경우 수분 함량을 70%로 유지하고 사육 밀도를 15만 마리로 설정하는 것이 가장 효과적임을 확인하였다.

This paper, based on cultural memory theory, examines how the digital game Black Myth: Wukong reconstructs and perpetuates cultural memory by integrating classic narratives with interactive storytelling. It first elucidates the core concepts of cultural memory, analyzing how Journey to the West serves as a cultural stabilizer. Through Sun Wukong’s rebellious spirit and adventure-driven narrative, it engraves Chinese cultural values deeply within collective memory. Next, an interactive storytelling perspective is introduced, highlighting how new media transform audiences from passive recipients to co-creators of cultural memory through immersion and engagement. Taking Black Myth: Wukong as a case study, this paper deconstructs its multilayered narrative structure and the modern reimagining of cultural symbols, such as the “six senses,” to demonstrate how the game endows the classic text with renewed cultural significance. Finally, it examines the game’s technological innovations and market performance to argue the complementarity between interactive storytelling and classic narratives, revealing the potential of digital media to drive cultural memory’s transmission and innovation, offering a new perspective for future cultural dissemination.

To determine the differences in food quality between fish fed a low fish meal diet containing black soldier fly (Hermetia illucens) instead of fish meal and those fed a general fish meal diet, we analyzed the approximate components, mineral content, amino acid composition, fatty acid composition, color, and texture of Flounder (Paralichthys olivaceus). The analysis of approximate components showed no difference between the two samples, except for moisture content. Mineral contents were measured in the order of K, P, Na, and Mg in both samples, with no difference except for phosphorus (P). A total of 16 amino acids were detected in both samples, with no significant differences in their composition. Additionally, 17 types of free amino acids were identified, with no significant differences between the two samples. The fatty acid composition consisted of 13 fatty acids, with palmitic acid, DHA, and oleic acid being the most prominent, although slight differences in content were observed. The color and texture also showed no differences between the two samples. Overall, there were no significant differences in chemical components or physical characteristics, so it was judged to be insignificant in terms of food science.

벼의 주요 해충인 먹노린재의 실내 사육을 위해 발육 특성을 조사하였다. 먼저 알을 부화시키기 위해서는 수분 제공이 반드시 필요하였다. 부 화한 1령 약충은 수분 제공만으로 2령 약충으로 발육이 가능하였다. 그러나 2령 약충은 수분 제공만으로는 3령 약충으로 발육하지 못하고 모두 사망 하였다. 먹노린재 약충 1령기부터 볏과 8종의 식물을 제공했을 때 출수기의 벼에서 약충부터 성충으로 도달한 개체의 생존율은 90.0%로 가장 높았 으며, 수수와 밀은 각각 30.0%, 26.7%였으며, 보리는 6.7%, 조와 피는 3.3%로 낮았다. 유묘기의 옥수수에서는 3령이 되기 전에 모두 사망하였고, 출수기의 기장에서는 성충이 되기 전에 모두 사망하였다. 유묘기의 벼에서는 성충에 도달한 개체의 생존율이 25%로 출수기의 벼보다 낮았다. 그러 나, 유묘기의 벼에서 자란 1~2령 약충은 출수기 벼와 다른 볏과 식물보다 체중이 가장 무거웠다. 출수기의 벼에서는 4령과 5령 약충의 발육기간이 각각 평균 8.5일과 12.1일로 가장 짧았다. 출수기 벼에서 자란 먹노린재의 성충 몸무게는 50.91 mg으로 유묘기의 벼와 출수기의 수수와 밀에서 자 란 먹노린재의 성충 몸무게보다 무거웠다. 이러한 결과를 종합하면 먹노린재 약충의 발육과 생존에 가장 적합한 기주식물로 출수기의 벼가 제안되 었다.

To utilize pepper (Piper nigrum) as an immunostimulatory agent, we isolated macrophage stimulating polysaccharides from pepper and investigated their macrophage activating activities. Hot-water extracts (HW) of black pepper (BP) and white pepper (WP) were prepared, and their macrophage stimulating activities were evaluated using RAW 264.7 cells. BP-HW significantly promoted the secretion of macrophage stimulating factors such as nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin (IL)-6, and IL-12 compared to WP-HW. When BP and WP-HW were fractionated into crude polysaccharides (CP) and low molecules (LM) by ethanol precipitation, BP-CP demonstrated significantly more potent activity. Furthermore, BP-CP not only induced mRNA gene expression of macrophage activation factors, but also promoted nuclear localization of p65 and c-Jun. In addition, component sugar analysis revealed that glucan-type polysaccharides in BP-CP played a crucial role in macrophage activation. Taken together, these findings suggest that black pepper has industrial applicability not only as a spice, but also as an immunostimulatory functional material.

2019년 12월, 상주-영천 고속도로 상행선에서 도로 노면 결빙에 의한 연쇄추돌사고로 48명의 사상자가 발생하였다. 이에, 국토교통부 는 2020년 1월 결빙 취약구간 선정기준을 마련하여 결빙 취약구간 403개소를 지정하고, 결빙 취약구간을 대상으로 2022년까지 1,699억 원의 예산을 투입하여 결빙사고 예방사업을 계획하였다(BAI, 2021). 하지만, 결빙 취약구간 선정기준에 대해 적정성 검토가 이루어지 지 않아 그 신뢰성과 실효성이 충분히 검증되지 않았다. 본 연구에서는 국가교통정보센터의 노드·링크(Node·Link) 체계를 기반으로 전국 고속국도 및 일반국도의 특성정보(시설, 선형구조, 기상, 교통 등)를 GIS(Geographic Information System) 데이터로 구축하였다. 최근 5년 결빙사고 발생이력이 있는 도로구간(Link)을 확인하고 Random Forest 알고리즘을 통해 도로 특성정보의 결빙사고에 대한 변수 중요도(Feature Importance)를 분석했다. 이를 통해 결빙사고와 각 인자의 상관성을 파악하여 ‘결빙 취약구간 평가 세부 배점표’의 항목별 배점을 수정, 보완함으로써 평가표의 신뢰성을 제고한다.

Present study investigated fish community at 12 sites within Yudeungcheon Stream in October 2018 and May 2019 to understand the inhabit status of the endangered species Pseudopungtungia nigra. We collected 39 species and 2,554 individuals of fish, and the Fish Assessment Index (FAI) was evaluated as “Good (B)” with an average score of 66.8. Among collected fishes, 15 endemic and 2 invasive species, Micropterus salmoides and Lepomis macrochirus were identified. In the distribution survey of P. nigra, it appeared in a range of approximately 12 km from Maebawiyuwonji Park (St. 5) to Budnaedari Bridge (St. 10). Based on population collected in May, the age of P. nigra (female) were divided into three groups by the length-frequency distribution method: 1 year old (60~76 mm), 2 years old (82~99 mm), and more than 3 years old (100~117 mm). Total length of the minimum maturity individual was 60 mm, and was a 1 year old. The estimated average population size using the Jolly-Seber method was 5,804 individuals in the Suryeongyo Bridge. Our study provides fundamental information about the fish community and River health assessment in Yudeungcheon Stream. Furthermore, we are expected to provide ecological information of the P. nigra, consequently helping to establish conservation and restoration plans for endangered species.

Black ice, a thin and nearly invisible ice layer on roads and pavements, poses a significant danger to drivers and pedestrians during winter due to its transparency. We propose an efficient black ice detection system and technique utilizing Global Positioning System (GPS)-reflected signals. This system consists of a GPS antenna and receiver configured to measure the power of GPS L1 band signal strength. The GPS receiver system was designed to measure the signal power of the Right-Handed Circular Polarization (RHCP) and Left-Handed Circular Polarization (LHCP) from direct and reflected signals using two GPS antennas. Field experiments for GPS LHCP and RHCP reflection measurements were conducted at two distinct sites. We present a Normalized Polarized Reflection Index (NPRI) as a methodological approach for determining the presence of black ice on road surfaces. The field experiments at both sites successfully detected black ice on asphalt roads, indicated by NPRI values greater than 0.1 for elevation angles between 45o and 55o. Our findings demonstrate the potential of the proposed GPS-based system as a cost-effective and scalable solution for large-scale black ice detection, significantly enhancing road safety in cold climates. The scientific significance of this study lies in its novel application of GPS reflection signals for environmental monitoring, offering a new approach that can be integrated into existing GPS infrastructure to detect widespread black ice in real-time.

Supplementing feed components is becoming increasingly difficult for various reasons, including increased shipping costs, decreased capture efficiency, and global warming. For this reason, much research has been conducted globally to find alternative protein sources. The black soldier fly (BSF; Hermetia illucens) is an important feed item for nutritional accumulation and has environmental consequences. Few studies have been conducted to determine the nutritional level of the substrate, but most of these studies have involved modifying the substrate material. The aim of this study was to determine the ideal crude protein(CP) content of a substrate for BSF rearing. The experimental treatment was separated into three CP levels (low, 13.5%; medium, 20%; high, 26.5%) in the substrate. The results showed that the BSF weight (6.46%) and protein conversion ratio (1.13%) increased as the substrate CP level increased (p<0.05). In addition, protein (14.38%) and lipid (13.12%) yields were significantly increased (p<0.05). The BSF CP level increased as the substrate CP level increased (p<0.05). CP levels in the substrate upregulated the levels of certain fatty acids, essential amino acids, and non-essential amino acids (p<0.05). In conclusion, the potential values of BSF as feed were improved as the CP level of the substrate increased; However, no difference was found between medium and high CP levels in the rearing substrate. These results suggested that the substrate CP level should be at the medium level for optimal product quality. In conclusion, BSF quality is influenced by substrate nutrient levels, necessitating the development of specialized substrates for effective rearing.

For sustainable livestock industry, black soldier fly as eco-friendly animal protein source is spotlighted for swine and poultry. However, most of black soldier fly is reared in dirty condition like food waste. In this study, we investigated the effects of fiber level on the growth performance and nutrient composition of black soldier fly reared in specific substrate. Treatments were divided in two as Low (fiber level: 7.1%), and High (fiber level: 14.2%) with a total of 50,000 eggs each. The rearing box had a dimension with 1064 × 507 × 320 cm (length × width × height). Black soldier fly survival rate (72.24 vs. 76.48) and substrate consumption (77.43 vs. 80.37) were greater in High than Low. However, bioconversion (10.59 vs. 9.80) and protein conversion (8.97 vs. 8.00) were increased in Low than High. Gross energy was higher in High compared with Low (4,133 vs. 4,208). Some of essential amino acid (Isoleucine, leucine, phenylalanine, threonine, tryptophan) were significantly higher in High compared with Low, and non-essential amino acid (alanine, aspartate, glycine, proline, serine, tyrosine) were higher in High compared with Low. Acids such as C12:0, C14:0, C15:0, and C20:1 were higher in High compared with Low, while C18:4 was higher in Low compared with High. As a result, black soldier fly reared in high fiber substrate might be a preferred option for rearing and producing a reliable animal protein source for monogastric animal.

Black soldier fly (BSF, Hermetia illucens) has been noted as an excellent feed ingredient. However, there is limited information on rearing and processing technology. Thus, this study was conducted to evaluate the substrates for rearing BSF and the optimal processing method for BSF performance. Study separated as 2 experiment, BSF rearing and drying method(Exp 1.) and EP-processing method(exp 2.). During the study, 30 clutches were reared, with 10 per substrate. Three substrates, namely food waste (FW), tofu by-product (TF), and vegetable waste with two drying methods, namely hot air dry (AD) and microwave dry (MW) at expanding (EP) ratios of 5:5 and 7:3, were examined by evaluating their rearing performance, nutrient contents, in vitro digestibility and lipid oxidation stability during storage (0, 14 and 28 days). In experiment 1, the rearing substrates and drying methods were evaluated. Compared with that of conventional methods (FW, AD), the TF substrates and MW method showed higher dry matter contents (3.43%) and in vitro digestibility (1.62%) but lower ether extract contents(3.53%; p<0.05). However, the malondialdehyde (MDA) concentration under MW treatments decreased during storage (5.77%, 4.69% and 3.24%; p<0.05). In experiment 2, compared with that of the 7:3 EP-BSF ratio, the 5:5 EP-BSF ratio showed higher in vitro digestibility (2.70%) and lower MDA concentration during storage (19.19%, 7.96% and 6.42%; p<0.05). In conclusion, the optimal conditions for BSF rearing and ensuring product quality were TF substrates, MW methods and a 5:5 corn:BSF ratio. Therefore, the optimal conditions for producing EP-BSF can present an excellent feed ingredient alternative for swine feed.

최근 국내 겨울철 블랙아이스(Black Ice)로 인해 발생하는 교통사고가 증가하는 추세이며, 한국 도로교통공단 조사 결 과 2016～2020년 겨울철까지 블랙아이스로 인한 사고는 총 4,868건이며, 사상자는 8,938명인 것으로 조사 되었다. 도로상 태에 따라 건조대비 동결상태에서 교통사고 발생시 치사율이 43%로 높게 나타났다. 이러한 사고는 기온이 떨어지는 12 월부터 급증하여, 최저기온이 가장낮은 1월까지 증가한다. 블랙아이스는 도로에 쌓인 눈이 융해(해설)과 동시에 도로 위 각종 이물질과 결합 후 재동결하여 흑색 동결막을 형성하는 것을 말한다. 그 특성상 운전자가 차량내부에서 도로의 상태 를 쉽게 파악할 수 없으며 대부분의 운전자가 차량이 미끄러지기 시작함과 동시에 인지하여 사고가 발생하게 된다. 이에 본 연구에서는 기존 포장체의 미끄럼 저항도를 상태별로 비교 분석하였다. 포장체의 미끄럼 저항성 정도를 파악하기 위 해 영국식 미끄럼저항 시험기 (British Pendulum Tester ; BPT)를 사용하였으며, 포장체의 종류로는 일반적인 밀입도 아스팔트 포장, 배수성 아스팔트 포장, 그루빙(포장 표면에 일정한 규격의 홈을 형성)을 적용한 콘크리트 포장, 그루빙이 없는 콘크리트 포장을 적용하였다. 미끄럼저항 실험은 관련 KS규격 및 ASTM규격에 준하여 실시하되 블랙아이스를 모 사하기위하여 표면온도 영하 2～3℃ 샘플에 강우를 모사한 물을 분사하며 영하 9℃로 10분 동결 후 2mm강수량을 모사 한 수분을 재 분사한 후 시험을 실시하였다.

본 논문은 회분식 반응기에서 습식 산화법으로 합성한 칼륨 페레이트(VI)에 의한 난분해성 아조 염료Reactive Black 5의 분해 과정을 연구하는 것을 목적으로 한다. 수용액에서 RB5의 분해는 pH, Ferrate (VI) 투입량, 초기 농도, 수용액 온도 등 다양한 변수의 조건에서 연구되었다. RB5 경우에는 최대 분해 효율은 pH 7.0에서 63.2%가 달성되었으며, 이 실험 조건에서 얻은 kapp 값은 190.49 M-1s-1 으로 나타났다. 온도 또한 가장 중요한 매개 변수 중 하나로 연구되었으며, 그 결과로부터 온도(45°C까지)를 증가시키면 페레이트(VI)에 의한 아조 화합물 염료의 분해 효율이 증가하고, 온도가 45°C를 초과하면 분해 효율이 저하되는 것으로 나타났다.

Black chestnut (BC) was obtained through aging of fresh chestnut (FC) at 80℃ for 15 days. Proximate and mineral compositions along with colors of FC and BC were evaluated. With aging, moisture contents decreased by 50%, whereas sugar contents, carbohydrate contents, and calories increased. Contents of minerals (Fe, P, Ca, Na, Mg, K) were significantly higher in FC than in BC, showing an order of Mg < Ca < P < K in both FC and BC. Using a Hunter color system, it was found that lightness (L), redness (a), and yellowness (b) values of FC were higher than those of BC. Antioxidant and cytotoxic activities of hot water and ethanol (50, 80, 100%) extracts prepared from FC and BC were evaluated. Extraction yields were lower with FC than with BC. Among water and ethanol extracts, water extract showed the highest DPPH radical scavenging activity for both FC and BC. IC50 values for ABTS+ radical scavenging activities increased after aging. Cytotoxicities of FC and BC extracts were similar to each other. They were different against various cell lines (3T3, HeLa, and Sarcoma-180). These results suggest that BC could be used as a new processed food using chestnut.