Severe Fever with Thrombocytopenia Syndrome (SFTS) is a newly emerging tick-borne disease caused by the SFTS virus (SFTSV), which belongs to the phlebovirus in the Bunyaviridae family. SFTSV is enveloped with a tripartite ambisense RNA genome. The L segment encodes the viral RNA-dependent RNA polymerase, the M segment encodes the two glycoproteins, Gc and Gn, and the S segment encodes the nucleoprotein (NP) and the nonstructural protein (NSs). NP participates in ribonucleoprotein (RNP) packaging and commonly detected early after infection, suggesting that the N protein is possible to be used as a target antigen for early diagnosis of SFTSV infection. In this study, we analyzed a highly immunogenic multi-epitope using GnGc and NP genes from a consensus sequence of SFTSV strain isolated from infected patients in Korea. The selected genes are constructed to the expression vector plasmid pJHL65 and the recombinant plasmid vector was transformed into the Δasd Δlon ΔcpxR Salmonella Typhimurium attenuated strain JOL912 and the expression of these antigens was verified by immunoblotting assay. We observed the significant levels of systemic IgG and mucosal IgM responses against the JOL912-derived antigen in the immunized BALB/c mice. The level of CD3+CD4+, CD3+CD8+ T lymphocyte subpopulation and TNF-α were also highly regulated in splenic T cells re-stimulated in vitro with NP and Gn/Gc multi-epitope selected antigens. Therefore, immunized mice with NP and Gn/GC multi-epitope recombinant proteins of attenuated Salmonella delivery system elicited T cell-related immune response, inducing an effective immune response. In conclusion, the attenuated Salmonella expressing NP-GnGc multi-epitopes could be a novel vaccine candidate against the SFTS virus.

This study investigated the effect of manufacturing variables (including heating temperature) on the physicochemical properties of nanoemulsion delivery system (NDS) prepared with WPI/Inulin Maillard conjugate and to study how the physicochemical properties of NDS affected the bioaccessibility of lycopene. The functional properties of the WPI/Inulin Maillard conjugate were determined using the OPA method, interfacial tension, and EAI. The physicochemical and morphological properties of NDS were measured using Zetasizer and TEM, respectively. The bioaccessibility of lycopene in the WPI/Inulin Maillard conjugate based NDS was measured using a spectrophotometer. As the pH and heating temperature increased, the Maillard conjugation efficiency increased significantly (p<0.0001). The emulsifying properties of the WPI/Inulin Maillard conjugate were greater than those of WPI. A WPI/Inulin Maillard conjugate based NDS with a size of ~180 nm was observed in TEM images while the droplet size of the WPI/Inulin Maillard conjugate based NDS was smaller than that of the WPI based NDS. During in vitro digestion, no significant changes in the droplet size and PDI of NDS were observed in the mouth and stomach phases, whereas in the intestinal phase, the droplet size and PDI increased significantly (p<0.0001). Moreover, the bioaccessibility of lycopene in the WPI/Inulin Maillard conjugate based NDS significantly increased (p<0.0001), compared with that of the WPI based NDS. There was a significant (p<0.05) increase in the bioaccessibility of lycopene with a decrease in the interfacial tension and droplet size of NDS. In conclusion, WPI/Inulin Maillard conjugate based NDS can be used to enhance the bioaccessibility of lycopene.

This study aimed to develop a solid self-nanoemulsifying drug delivery system (solid-SNEDDS) to enhance the formulation of ketoconazole (KTZ), a BCS Class II drug with poor solubility. Ketoconazole, which is insoluble above pH 3, requires solubilization for effective delivery. This SNEDDS comprises oil, surfactant, and co-surfactant, which spontaneously emulsify in the gastrointestinal tract environment to form nanoemulsions with droplet sizes less than 100 nm. The optimal SNE-vehicle composition of oleic acid, TPGS, and PEG 400 at a 10:80:10 weight ratio was determined based on the smallest droplet size achieved. This composition was used to prepare liquid SNEDDS containing ketoconazole. The droplet size and polydispersity index (PDI) of the resulting liquid SNEDDS were analyzed. Subsequently, solid-SNEDDS was fabricated using a spray-drying method with solidifying carriers such as silicon dioxide, crospovidone, and magnesium alumetasilicate. The physicochemical properties of the solid-SNEDDS were characterized by scanning electron microscopy and powder X-ray diffraction, and its solubility, droplet size, and PDI were evaluated. In particular, the solid-SNEDDS containing ketoconazole and crospovidone in a 2:1 weight ratio exhibited significantly enhanced solubility, highlighting its potential for improved medication adherence and dissolution rates.

This study aims to prepare a colloidal silica-containing powder to enhance the solubility and dissolution rate of rivaroxaban using a self-nanoemulsifying drug delivery system (SNEDDS). We investigate the impact of colloidal silica on a nanoemulsion system for preparing powdered SNEDDS. The liquid SNEDDS comprises 30/20/50 (w/w/w) Peceol/ Cremophor RH40/Tween 80, which results in the formation of the smallest droplets. Three powdered SNEDDS formulations are prepared by suspending the liquid SNEDDS formulation using colloidal silica and spray drying. The powdered SNEDDS prepared with liquid SNEDDS and colloidal silica at a ratio of 1/0.5 (w/w) exhibits the highest water solubility (0.94 ± 0.62 vs. 26.70 ± 1.81 μg/mL) and dissolution rate (38.4 ± 3.6 vs. 85.5 ± 3.4%, 45 min) when compared to the drug alone. Morphologically, the liquid SNEDDS is adsorbed onto colloidal silica and forms smaller particles. In conclusion, an SNEDDS containing rivaroxaban, prepared using colloidal silica, facilitates the creation of a nanoemulsion and enhances the water solubility of rivaroxaban. Accordingly, this technology holds significant potential for commercialization.

The objective of this study was to produce a nutrient delivery system (NDS) using sialic acid extracted from edible bird’s nest (EBN), which improves brain function in patients with Alzheimer’s disease and Parkinson’s disease, by affinity bead technology (ABT). The inhibitory activity of acetylcholinesterase (AChE) and pyramidal cells in the dentate gyrus of the hippocampus were analyzed to investigate the effect of a sialic acid NDS on Alzheimer’s disease. Also, the effect of a sialic acid NDS on Parkinson’s disease was evaluated by rota-rod test and pole test in an animal model. Among the groups treated with donepezil, EBN, and sialic acid NDS, the AChE activity was the lowest in the sialic acid NDS-treated group. The results of the hippocampus analysis of the rat model confirmed that the sialic acid NDS inhibited amyloid-beta accumulation depending upon the concentration. Also, the sialic acid NDS group showed more improvement in motor deterioration than the1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)- induced group in both the rota-rod test and pole test. Therefore, the sialic acid NDS had an effect of protecting not only Alzheimer’s disease by inhibiting AChE and amyloid-beta accumulation, but Parkinson’s disease by preventing neurotoxicity induced by MPTP.

최적화 분야에서는 VRP(Vehicle Routing Problem)에 대한 많은 연구가 오래전부터 이루어져 왔다. 기본적인 VRP 모형을 확장하여, 단일차고지와 다수차고지 특성, Pickup and Delivery 특성, Time Windows 특성 등을 고려하여 다양한 모형들을 도출되고 있으며 이들에 대한 보다 나은 해를 구하는데 초점이 맞추어져 왔다. 이들 VRP 모형들은 노드 수의 증가에 따른 NP-Hard 특성을 가지고 있기 때문에 최적화에 초점을 맞추기 보다는 Genetic Algorithms, Tabu Search, Simulated Annealing 등의 메타휴리스틱 기법 등을 이용하여 적용할 수 있는 우수한 해를 도출하려 노력을 기울이고 있다. 본 연구에서는 시간제약을 가지는 Multi-Depot Pickup and Delivery 모형을 이용하여 3자물류 기업에서 물류 트럭의 할당 및 이들에 대한 운영 상황 모니터링을 지원할 수 있는 시스템 개발에 초점을 맞추고 있다. 본 연구에서는 먼저 수요기업의 요구사항을 반영하여 가장 중점을 두고자 하는 혼적이 가능하고 시간제약을 반영한 해를 도출하고자 했으며 도출된 해를 기반으로 물류를 트럭에 할당한 후 물류 트럭에 대한 GPS기반의 모니터링 시스템을 제안하고자 한다.

PURPOSES : This study aims to survey and analyze the status of the design and completion documents output delivery system in public construction projects in order to assess the problems of the Ministry of Land, Infrastructure and Transport (MOLIT)'s electronic delivery system, with a focus on roads and rivers, and to offer improvement measures. METHODS: This study first surveys and analyzes laws and existing study trends with regard to the design and completion documents output delivery system in public construction projects. It further analyzes the status of the output delivery system in public construction projects, including roads, rivers, expressways, water resources, and railroads. In addition, a comparative analysis of the current electronic delivery system of MOLIT and Korea Expressway Corporation in the road field is conducted, and the problems and improvement measures for MOLIT's electronic output delivery system are presented. This study seeks to analyze problems and prepare improvement measures with regard to road and river public construction projects, as ordered by MOLIT’s five regional offices. RESULTS: This study sought to prepare the electronic output delivery system with regard to public construction projects and present the analysis of its major problems and improvement measures in four categories: “the preparation of electronic design documents output delivery system improvement measures including inspection and delivery,”“preparation of guidelines, systems, etc., concerning electronic design documents,”“preparation of improvement measures for the construction project management system and electronic design document support tools”, and “linking of MOLIT's electronic output delivery system with relevant agencies and the expansion thereof to local governments.” CONCLUSIONS : This study analyzed the current status and problems of the MOLIT's electronic output delivery system in the field of road and river construction projects, and presented corresponding improvement measures. This study is expected to address the problems of the MOLIT's electronic output delivery system, and to provide the foundation for the preparation of an efficient electronic output delivery system for design and completion documents. In addition, this study is expected to boost the quality and utilization of the electronic design documents output, and to exchange, share, and link construction information among relevant agencies so as to prepare the foundation for sharing construction information.

본 연구의 목적은 국가치료기구를 설립해서 체계적인 모델의 틀을 만들어 사법권 제도 체제안과 지역사회에서 마약류 치료 서비스와 치료전달체계를 성공적으로 실시하여 마약류 남용자의 수를 감소시킨 영국사례를 고찰하여 한국에서 중점을 두고 있는 사법권 제도와 지역사회 안에서의 자발적인 치료 서비스와 사회복귀가 형식적인 시혜조치로 끝나는 것이 아니라 한국 실정에 맞는 실질적인 치료 서비스와 치료전달체계를 정립하는데 기여하고자 했다. 그 결과 다음과 같은 시사점을 제언하고자 한다. 첫째, 마약류 남용을 처벌이 아닌 질병의 대상으로 인식 시키는 제도적인 기틀이 마련되어야 한다. 둘째, 실질적인 국가 정책을 수립하여 지역사회중심으로 마약류 남용치료 센터를 구축해야 한다. 마지막으로 치료적 서비스 내용 개발로 한국 실정에 맞는 치료 서비스 내용 및 전달 체계의 확립과 치료인력의 구축을 들 수 있다.

In order to determine home delivery service routes, conditions specified for each parcel such as customer-assigned delivery times and parcel contents should be considered, so the conventional route search algorithms have some limits to be applied for home delivery services. In this study, a multi-purpose route searching algorithm is suggested in order to deal with every requirement of deliveries that vary in delivery distances, contents and appointed times. A simulation study to verify the performances of the system with example data of Seoul and Kyungki provinces shows that it significantly improves the customer satisfaction and the productivity of delivery businesses.

Door to door service is a typical method for individual parcel deliveries. In the current delivery information system, a delivery person manually inputs the information of many delivery results, which causes inefficiency and difficulties of tracking deliveries. This study suggests an enhanced delivery information system which has the following two characteristics. Firstly, a tagged RFID(Radio Frequency Identification) transmits the delivery result information to the main server by just collecting RFID at a place of destination. Secondly, with the characteristics of rewritable method, the collected RFID is to be input new information and tagged to other parcels.

Door to door service is a typical method for individual parcel deliveries. In the current delivery information system, a delivery person manually input the information of many delivery results, which causes inefficiency and difficulties of tracking deliveries. This study suggests an enhanced delivery information system which has the following two characteristics. Firstly, a tagged RFID transmits the delivery result information to the main server by just collecting RFID at a place of destination. Secondly, with the characteristics of rewritable method, the collected RFID is to be input new information and tagged to other parcels.

생체 적합성이 우수한 히아루론산과 생분해성이 우수한 폴리 락타이드의 이량체를 결합하여 약물 방출 시스템에 적용할 수 있는 생체 재료를 제조하고자 하였다. 냉동 건조법을 이용하여 히아루론산과 락타이드를 가교제 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC)로 가교시켰다. 생성된 막을 핵자기 공명 분광법으로 분석하여 락타이드 반응도와 EDC 반응도를 결정하였다. 히아루론산에 대한 락타이드 몰비, 가교제 농도가 증가할수록 혹은 가교 온도가 감소할수록, 락타이드 반응도가 증가하였으며 팽윤도는 감소하였다. 서로 다른 락타이드 반응도를 가진 막으로 약물 방출 실험을 수행한 결과 락타이드 반응도가 증가하면 약물 방출 속도가 감소하는 경향을 보였다. 또 친수성이 다른 여러 가지 약물로 약물 방출 실험을 수행한 결과 친수성이 우수한 약물일수록 서서히 방출되었다.

Many research papers on transportation studies have focused on the Vehicle Routing Problem (VRP) and Vehicle Scheduling Problem (VSP). However in the real world, it is known that it takes long time to build vehicle scheduling in the process of transporting the amount of orders from the logistics center to the vendors due to the realistic constraints. This paper presents a framework design for each process enabling delivery planning automatically using heuristic algorithm. In addition, an interactive automatic delivery planning system is implemented utilizing the proposed algorithm.

It is very important to delivery timely and safely items in logistics. So, the home delivery service system is introduced by many enterprises. Also, the beginning in 1990's, the rapid increase of automobile increased logistics cost and led to road stagnation. So, the railway transportation use in logistics can be considered. The railway transportation in home delivery service system can overcome the limit of transportation by land. This study evaluate railway transportation in home delivery service system by economical efficiency and timeliness.