Oil spills have occurred throughout the years of industrialization and represent a global challenge as they affect vast areas of the ocean. The toxicity of crude oil to aquatic organisms has been extensively investigated, but the potential impacts of crude oil on vertebrate development remain largely unknown. Here, we investigated the effects of dispersants used in treating a recent oil spill, as well as that of crude oil, on vertebrates by using the zebrafish (Danio rerio) model species, which has been widely used in empirical studies of both early embryonic development and adult physiology. Chronic exposure to crude oil resulted in marked developmental abnormalities, including pericardial edema, abnormal trunk vessel development, retardation of axonal branching, and abnormal jaw development. Embryonic development was affected more severely by exposure to the oil-dispersant combination than to the oil alone. Thus, the zebrafish in vivo model system suggests that dispersant treatment can have detrimental developmental effects on vertebrates and its potential impact on marine life, as well as humans, should be carefully considered in clean-up efforts at the site of an oil spill.

바다에 기름오염 사고가 발생하면 여러 가지 방제 방법 중 물리적 회수 방법을 우선적으로 사용하고 유처리제는 최후 수단으로 고려하는 경향이 있다. 유처리제는 수중으로 기름이 신속히 분산되도록 하여 해수면으로부터 제거하는 방법이다. 해수면으로부터 신속히 기름을 제거하는데 대한 유처리제의 효용성은 널리 증명되어 왔으나 아직도 대부분의 국가들은 해양환경에 미치는 독성을 우려하여 적극적인 사용을 하지 않고 있는 실정이다. 보고된 자료에 의하면 유처리제와 혼합된 기름이 기름 그 자체보다 독성이 더 크게 나타나지 않았다. 멕시코만 기름유출 사고시 미국 정부와 BP사는 최대한 해안에 기름이 도달하지 않는데 중점을 두고 해수면뿐만 아니라 수중의 기름에 대해서도 유처리제를 사용하였다. 유처리제에 대한 순환경편익을 분석하면 유처리제를 사용함으로써 기름이 생태계에 머무는 시간이 줄어들며 장기간 노출을 예방하고 야생동물에 심각한 오염을 방지하는 효과가 있는 등 다양한 연구가 진행되고 있다. 미국 멕시코만 유류오염 사고와 같은 대규모 해양오염사고의 위험이 상존하는 우리 실정에서도 과학적 결과를 바탕으로 한 유처리제 사용의 효용성과 안전성에 대한 검토가 이루어져야 할 시점이라 사료된다.

바다에 기름오염 사고가 발생하면 여러 가지 방제 방법 중 물리적 회수 방법을 우선적으로 사용하고 유처리제는 최후 수단으로 고려하는 경향이 있다. 유처리제는 수중으로 기름이 신속히 분산되도록 하여 해수면으로부터 제거하는 방법이다. 해수면으로부터 신속히 기름을 제거하는데 대한 유처리제의 효용성은 널리 증명되어 왔으나 아직도 대부분의 국가들은 해양환경에 미치는 독성을 우려하여 적극적인 사용을 하지 않고 있는 실정이다. 보고된 자료에 의하면 유처리제 사용 후 수중생물에 대한 독성은 발견되지 않았으며, 유처리제와 혼합된 기름이 기 름 그 자체보다 독성이 더 크게 나타나지 않았다. 멕시코만 기름유출 사고 시 미국 정부와 BP사는 최대한 해안에 기름이 도달하지 않는데 중 점을 두고 해수면뿐만 아니라 수중의 기름에 대해서도 유처리제를 사용하였다.

The amorphous (at%) alloy strip was pulverized using a jet mill and an attrition mill to get flake-shaped powder. The flake powder was mixed with dielectric powder and its dispersant to increase the permittivity. The powders covered with dielectric powders and its dispersant were mixed with a binder and a solvent and then tape-cast to form sheets. The absorbing properties of the sheets were measured to investigate the roles of the dielectric powder and its dispersant. The results showed that the addition of powders and its dispersant improved the absorbing properties of the sheets noticeably. The powder sheet mixed with 5 wt% of powder and 1 wt% of dispersant showed the best electromagnetic wave absorption rate because of the increase of the permittivity and the electrical resistance

Poly(oxypropylene-oxyethylene glycol) block copolymer(PBC) oil dispersant, which has low toxicity, high biodegradability, and an excellent dispersion efficiency to crude oils and weathered W/O emulsion was prepared by blending PBC, poly(oxyethylene) oleate, and sorbitan monooleate. The dispersing efficiency was measured by swirling flask method. The PBC oil dispersant had an excellent dispersing efficiency to weathered oil products formed as stable W/O emulsion, and the low toxicity, such as 4000 ppm to Oryzias Latipes(24 hr, TLM), Brine Shrimp Artemia(24 hr, TLM).

Demand for organic analysis increase as industries are growing and many products are spreaded in the daily life. One of many products is oil spill dispersant. It was used for oil accident in the ocean. When oil spill dispersant spread at the ocean, the petroleum in the ocean is dispersed. The oil spill dispersant is made of non ionic surfactant and petroleum oil. The non ionic surfactant disperse petroleum from oil accident. The other part is petroleum oil which has aromatic hydrocarbon. Because the aromatic hydrocarbon is cancerogenic material, it directly injure animals in the ocean. This cause the second pollution in the human body. Many oil accidents still are controlled by oil spill dispersant. Therefore quality control of the oil spill dispersant become important and this also demand for the exact quantitative analysis of aromatic hydrocarbon. Hereupon the first we develop separate petroleum oil from surfactant. The second standardize analytical method of aromatic hydrocarbon in the separated petroleum oil.

Polyoxyethylene monooleate was prepared by addition of ethylene oxide to oleic acid. And also, polyoxyethylene monooleate type oil dispersant was prepared by blending polyoxyethylene monooleate, n-paraffine, sorbitan monooleate, sorbitan monopalmitate, and palm oil. Dispersion efficiency test was carried out by vertical shaking flask and swirling flask methods. Low toxic oil dispersant was prepared with polyoxyethylene monooleate, which has high biodegradability and excellent dispersion efficiency on crude oils and weathered W/O emulsions with high viscosity, and its dispersion efficiency was measured to various crude oils and weathered oils.

Low toxic concentrated oil dispersant using n-Paraffin and Di(ethylene glycol)mono butylether mixed solvent was prepared, and tested by oil dispersant performance test method, and oil dispersant efficiency was measured using vertical shaking method to 3 kinds of Crude oil, Bunker oil and W/O emulsions with different physical properties by appling the prepared dispersant. Although toxicity test was performed with Flat fish and Rock fish by appling the mixed oils emulsified using prepared oil dispersant, couldn't find the toxicity to them. Concentrated oil dispersant prepared has a good dispersion efficiency of 97.2% after 0.5min settling time and 28.3% after 10min settling time to Bunker B oil with 10% water solution. Especially, the concentrated oil dispersant showing the low toxicity to Oryzias Latipes(24hr, TLm) was 54,000 ppm and to Brine Shrimp Artemia(24hr, TLm) was 51,000ppm, and also, it was completely biodegradated to 99.1% after 7~8days.

The in vitro toxicities of three crude oils of the Hebei Spirit were examined on laboratory grown plankton, with a focus on the effects of a dispersant. The specific growth rate of phytoplankton and the mortalities of two zooplankton were measured in response to exposure to various concentrations of water accommodated oil, dispersant or both. The effects of the oils varied among the plankton, but were generally low within the range of the oil concentrations used, with little difference in toxicity among the three oils. Such low toxicity appeared to be associated with weathering of the crude oils. Exposure to the dispersant, however, dramatically increased the mortality of zooplankton, with complete inhibition of phytoplankton growth. No synergistic toxic effect was observed with the crude oil and dispersant combination. A better decision making process could be crafted for future application of dispersant in the event of an oil spill in Korean waters to better protect the marine plankton community from the excessive use of dispersant.

Oil spill caused severe effects on the marine fauna and flora due to direct contact of organisms with the oil and even in regions not directly affected by the spill. This study was conducted to understand the effects of the oil spill accidents and the use of dispersant on the red tide of Cochlodinium polykrikoides. Crude oil produced in Kuwait, bunker-C, kerosene and diesel oil, and a chemical dispersant produced in Korea, were added with a series of 10 ppb to 100 ppm in the f/2-Si medium at 20℃ under a photon flux from cool white fluorescent tubes of 100 mol m-2 s-1 in a 14: 10 h L:D cycle for the culture of C. polykrikoides. In low concentrations of ≤1 ppm of examined oils no impact on the growth of C. polykrikoides was recorded, while in high concentration of ≥10 ppm, cell density was significantly decreased with the range of 10 to 80% in comparison with the control. The growth of C. polykrikoides after the addition of the dispersant and the mixtures combined with oils and a dispersant of ≥10 ppm appeared to decrease, whereas the growth of C. polykrikoides exposed to ≤100 ppb showed little serious impact. However, almost all the C. polykrikoides cells were died regardless of a dispersant and combined mixtures within a few days after the addition of high concentrations.