Inflorescence, stem, and leaf samples of lettuce grown in a greenhouse in spring and autumn seasons were assayed for sesquiterpene lactones (SLs) content by high performance liquid chromatography. The concentrations of SLs were significantly higher in the inflorescences followed by upper leaf and stem compared to the other plant parts in most of the samples. SLs content (sum of lactucin and lactucopicrin) in various tissues of lettuce cultivated in spring season varied from 5.7 to 22.5 fold ranging from 27.4 ㎍/g dry weight (DW) in the upper stem (cultivar “PI 176588”) as the lowest to as high as 2,292.0 ㎍/g DW in the inflorescence (cultivar “709849-1”). During autumn cultivation, the concentration of SLs varied from 2.0 to 14.4 fold ranging from as low of 32.4 ㎍/g DW in the lower stem (cultivar “PI176588”) to as high of 838.0 ㎍/g DW in the upper leaf (cultivar “Dambaesangchu”). Higher lactucin (1.2 to 5.6 fold) and lactucopicrin (1.1 to 3.9 fold) concentration was observed during spring compared to autumn cultivation in most of the samples. SLs content in various organs of lettuce increases from the basal plant part going upwards. As lactucin and lactucopicrin are the major SLs which affects the sensory property of lettuce, their quantitative variation in the lettuce cultivars is useful for breeding new varieties with better consumer acceptance.

The author undertook PCR-founded genetic platform to investigate the hierarchical dendrogram of Euclidean genetic distances of one razor clam population, particularly for Solen corneus, which was further associated with those of the other clam population, by engaging with the precisely designed oligonucleotide primer sets. Seven oligonucleotides primers were used producing a total of 639 counted bands in population A and 595 in population B, respectively, ranging in size of DNA fragments from larger than approximately 50 bp to less than 1,100 bp. Their primers generated 39 specific fragments (6.10%) in population A and 47 (7.90%) in population B, respectively Comparatively, individuals of one razor clam population were fairly related to that of the other clam population, as shown in the hierarchical dendrogram of Euclidean genetic distances. The analysis of genetic variation between razor clam populations could offer important statistics for fisheries and mariculture. Generally the results showed specific and/or conserved genetic loci between razor clam populations. Specific markers established by the author will be valuable for the genetic analysis, species protection and increase of razor clam individuals in coastal region of the Korean Peninsula.

Genomic DNA samples isolated from geographical purplish Washington clam (Saxidomus purpuratus) were obtained from three different regions in the Korean Peninsula: Geoje (Geoje population; GJP), Gunsan (Gunsan population; GSP) and a site of North Korea (North Korea population; NKP). The seven primers generated the total 369 loci that can be scored from the GSP clam population. 356 fragments were generated from the NKP clam population. The complexity of the banding patterns varies dramatically between the primers and three localities. In this study, 319 loci were identified in the purplish Washington clam from Geoje and 369 in the clam population from Gunsan: 221 specific loci (69.3%) in the GJP clam population and 300 (81.3%) in the GSP population. These results demonstrate that the primer detected a large quantity of specific fragments, suggesting that the genetic variation in the GSP is higher than in the GJP population. In particular, the BION-28 primer gave DNA profiles with more fragments than the other six primers in the NKP population. The oligonucleotides primer BION-75 produced 21 unique loci to each population, which were ascertaining each population, approximately 250 bp, 300 bp and 400 bp, in the GJP population. Outstandingly, the primer BION-50 detected 21 shared loci by the three populations, major and/or minor fragments of sizes 150 bp, which were matching in all samples. With regard to average bandsharing value (BS) results, individuals from GJP population (0.743) displayed higher bandsharing values than did individuals from GSP population (0.606). In the present study, the dendrogram gained by the seven oligonucleotides primers indicates three genetic clusters: cluster 1 (GEOJE 01 ~ GEOJE 07), cluster 2 (GUNSAN 08 ~ GUNSAN 14), cluster 3 (N.KOREA 15 ~ N.KOREA 21). Among the twenty one clams, the shortest genetic distance that revealed significant molecular differences was between individuals 08 and 09 from the NKP population (genetic distance = 0.073), while the longest genetic distance among the twenty-one individuals that demonstrated significant molecular differences was between individuals GEOJE no. 03 and GUNSAN no. 09 (genetic distance = 0.669). Comparatively, individuals of GJP population were properly closely related to that of NKP population, as revealed in the hierarchical dendrogram of genetic distances. In due course, PCR analysis has revealed the significant genetic distance among three purplish Washington clam populations. PCR fragments discovered in this study could be valuable as a DNA marker of the three geographical clam populations to distinguish.

국내 밀 수량성 향상을 위해 이삭 길이가 길고 1수립수가 많은 장수형 계통의 품질과 이들 특성과 관련된 표지인자에 대한 평가를 수행하였다. 장수형 164계통의 회분, 단백질, 침전가, 습부율, 밀가루 입자 크기와 밀가루 색택 밝기의 평균은 금강밀보다 회분과 단백질함량이 높으며 습부율은 높고, 밀가루 밝기와 입자 크기는 비슷하였으나 침전가는 낮게 나타났다. 단백질의 질적 특성에서 단백질함량은 침전가, 습부율, 밀가루 입자 크기와 고도의 정의 상관을 보였고, 밀가루의 밝기와는 부의 상관을 나타냈다. 품질특성 관련 표지인자를 평가한 결과, 장수형 164계통은 모두 Glu-A3c, Wx-A1a, Wx-B1a, Wx-D1a의 유전자형을 나타내 금강밀과 동일하였다. 장수형 계통은 주로 Glu-A1c, Glu-B1c, Glu-D1a, Glu-B3g, Pina-D1b와 Pinb-D1b의 유전자형이 많았다. Glu-A1b과 Glu-B1b 유전자형은 단백질함량이 많고, 습부율이 높은 특성을 나타냈다. Glu-D1d, Glu-B3h과 Pinb-D1b 유전자형은 다른 유전자형에 비해 회분과 단백질함량이 많으며, 침전가와 습부율이 높고, 밀가루 입자가 크고, 밀가루의 색이 어두운 특성을 보였다. 이러한 결과를 통해서 장수형 계통은 수량성 증대를 위해 필요하고, 향후 용도별 품질 우수 자원의 특성을 집적하여 용도별 다수성 밀 품종개발을 위한 자원으로 활용하고자 한다.

국내 밀의 수량 증진을 위해 이삭 길이가 길고 1수립수가 많은 장수형 164계통(F8)을 육성하였고, 본 연구에서는 이들 계통의 주요 농업형질과 이들 특성과 관련 있는 표지인자에 대한 평가를 수행하였다. 장수형 계통은 모본인 금강밀과 비교했을 때, 단위면적당 수수와 리터중은 적고, 파종 후 출수까지 일수가 길었고, 간장, 수장, 1수립수와 단위면적당 생산량은 높게 나타났다. 농업 특성 관련 표지인자를 평가한 결과, 장수형 계통은 Rht-B1, Rht-D1, Vrn-B1, Ppd-A1, Ppd-B1 유전자 좌에서는 같은 유전자를 지닌 것으로 나타났다. 장수형 계통 중 Vrn-D1를 지닌 계통은 Vrn-D1a를 지닌 계통에 비하여 출수에 걸리는 시간이 길었지만, 이삭길이와 1수립수가 많은 것으로 나타났다. 하지만 Vrn-B1 유전자 변이는 장수형 계통의 농업형질의 차이가 없는 것으로 나타났다. 장수형은 Ppd-D1유전자 변이에 따른 간장이나 이삭 길이는 차이가 없었지만, 출수 일수와 수수는 Ppd-D1b를 지닌 계통이 많았지만, 1수립수, 천립중, 리터중과 수량은 Ppd-D1a를 지닌 계통이 높았다. 종실 특성을 나타내는 표지인자 변이는 출수 일수, 간장, 수장, 분얼이나 1수립수 및 수량에는 영향을 주지 않았지만 TaSus2-2B유전자의 Hap-L타입, TaGW2유전자의 Hap-6A-G타입과 TaCwi-A1a 가 각각의 대립 유전자형보다 천립중이 높게 나타났다. 향후 재배환경이 장수형의 농업형질 및 품질에 미치는 영향에 대한 평가가 필요하고, 만숙과 분얼이 적은 장수형의 단점을 보완하기 위한 지속적인 연구가 필요하다.

Next generation sequencing (NGS) technologies provide a fast and easy way to understand the plant genomes, transcriptomes, regulatory elements and their interactions. About a decade ago, rice was the only crop that whole genome sequence information was publicly available but today many agricultural crops including maize, soybean, tomato, potato, cotton have been sequenced and many more will be available. Moreover newly developed method such as Genotyping-By-Sequencing (GBS) allows efficiently collecting sequence information from hundreds of individuals in population to identify genetic variations, detect quantitative trait loci (QTLs) and develop molecular markers. Coupled with high-throughput phenotyping, the accumulated genomic information will be effectively utilized in crop improvement by genomics-assisted breeding, genome-wide association mapping (GWAS) and genomic selection (GS). Rice is one of the important staple crops providing daily nutrition to more than a half of the world population. The genus Oryza consists of 23 species including two domesticated rice and it has been classified into 10 distinct genome types, represented by six diploids (A, B, C, E, F, and G) and four allotetraploids (BBCC, CCDD, HHJJ, and HHKK). It shows wide ranges of phenotypic variations to biotic and abiotic stress thus is considered a genetic reservoir of unique allelic variation for rice improvement. International collaborative efforts have been focused on generating the Oryza genomics resources including reference genome, transcriptome, smallRNAs, methylome and resequencing of many accessions to collect genetic variations and better understand the 15 MY evolution of Oryza. The Oryza genomic resources will be a backbone to layer various omics data to catalogue more genetic variations within and between Oryza species and the untapped genetic diversities existing in wild Oryza species will be finally translated to crop improvement.

In this study, seven oligonucleotides primers were shown to generate the shared loci, specific loci, unique shared loci to each species and shared loci by the three species which could be obviously calculated. Euclidean genetic distances within- and between-species were also calculated by complete linkage method with the sustenance of the hierarchical dendrogram program Systat version 13. The genomic DNA isolated from herring (Clupea pallasii), Korean anchovy (Coilia nasus) and large-eyed herring (Harengula zunashi), respectively, in the Yellow Sea, were amplified several times by PCR reaction. The hierarchical dendrogram shows three chief branches: cluster 1 (PALLASII 01, 02, 03, 04, 06 and 07), cluster 2 (NASUS 08, 09, 10, 11, 12, 13 and 14), and cluster 3 (ZUNASHI 15, 16, 17, 18, 19, 20, 21 and PALLASII 05). In three clupeid species, the shortest genetic distance displaying significant molecular difference was between individual PALLASII no. 03 and PALLASII no. 02 (0.018). Individual no. 06 of PALLASII was most distantly related to NASUS no. 11 (genetic distance = 0.318). Individuals from herring (C. pallasii) species (0.920) exhibited higher bandsharing values than did individuals from Korean anchovy (C. nasus) species (0.872) (P<0.05). As a result, this PCR analysis generated on the genetic data displayed that the herring (C. pallasii) species was widely separated from Korean anchovy (C. nasus) species. Reversely, individuals of Korean anchovy (C. nasus) species were a little closely related to those of large-eyed herring (H. zunashi) species.

In the present study, muscle tissues were obtained separately from individuals from Atlantic hairtail population (AHP), Gunsan hairtail population (GHP) and Chinese hairtail population (CHP), respectively. The seven decamer primers were used to generate the shared loci, specific, unique shared loci to each population and shared loci by the three hairtail populations. Here, averagely, a decamer primer generated 64.7 amplified products per primer in the AHP population, 55.7 in GHP population and 56.4 in CHP population. The number of unique shared loci to each population and number of shared loci by the three populations generated by genetic analysis using 7 decamer primers in AHP, GHP and CHP population. 119 unique shared loci to each population, with an average of 17 per primer, were observed in the AHP population, and 28 loci, with an average of 4 per primer, were observed in the CHP population. The hierarchical dendrogram point out three main branches: cluster 1 (ATLANTIC 01 ~ ATLANTIC 07), cluster 2 (GUNSAN 08 ~ GUNSAN 14) and cluster 3 (CHINESE 15 ~ CHINESE 21). The shortest genetic distance displaying significant molecular difference was between individuals' CHINESE no. 16 and CHINESE no. 18 (0.045). In the long run, individual no. 01 of the AHP population was most distantly related to CHINESE no. 19 (genetic distance = 0.430). Consequently, PCR analysis generated on the genetic data displayed that the geographic AHP population was widely separated from CHP population, while individuals of CHP population were fairly closely related to those of GHP population.

Hairtails (Trichiurus lepturus) are the most popular marine products in Korea because of their taste and nutritional value, and Koreans consume them in large quantities. Hairtail, ecologically important warm water fish species, belonging to family Trichiuridae, widely distributed on the coast of the West Sea, South Sea and Jeju Island in the Korean Peninsula and the several sea areas in China under the natural ecosystem. However, in spite of their economic and scientific consequences, a little information currently exist regarding the physiological and ecological levels only of hairtail species in Korea (Koo et al., 2004). Simply the biological fisheries feature, distribution and migration of hairtail (T. lepturus) in Korean waters were surveyed (Park et al., 2005). Currently, imported hairtail have been altered into endemic hairtail because of high edge. In the present study, to explicate the genetic distances and differences among geographical hairtail populations, we accomplished a clustering analysis of three hairtail populations collected from Atlantic, Korea and Chinese site. Muscle tissues were obtained separately from individuals from Atlantic hairtail population (AHP), Gunsan hairtail population (GHP) and Chinese hairtail population (CHP), respectively. The muscle was collected in sterile tubes, immediately placed on dry ice, and stored at －40℃ until the genomic DNA extraction. Genomic DNA was extracted and purified under the conditions described previously (Yoon and Kim, 2004). Seven primers (BION-02, BION-03, BION-04, BION-08, BION-09, BION-13 and BION-17) were shown to generate the shared loci, specific loci, unique shared loci to each population and shared loci by the three populations which could be obviously scored. The degree of variability was calculated by use of the Dice coefficient (F), which is given by the formula: F = 2 nab / (na+nb), where nab is the number of bands shared between the samples a and b, na is the total number of bands for sample a and nb is the total number of bands for sample b (Jeffreys and Morton, 1987; Yoke-Kqueen and Radu, 2006). Euclidean genetic distances within- and between-population were also calculated by complete linkage method with the support of the hierarchical dendrogram program Systat version 13 (SPSS Inc., Chicago, IL, USA). Here, the seven decamer primers BION-02, BION-03, BION-04, BION-08, BION-09, BION-13 and BION-17 were used to generate the shared loci, specific, unique shared loci to each population and shared loci by the three populations. In the present study, averagely, a decamer primer generated 64.7 amplified products per primer in the AHP population, 55.7 in GHP population and 56.4 in CHP population. The number of unique shared loci to each population and number of shared loci by the four populations generated by genetic analysis using 7 decamer primers in AHP, GHP and CHP population. 119 unique shared loci to each population, with an average of 17 per primer, were observed in the AHP population, and 28 loci, with an average of 4 per primer, were observed in the CHP population. Many researchers studied the sizes of DNA fragments in the PCR profiles of five species of Eastern Pacific abalone (genus Haliotis) (Muchmore et al., 1998), black tiger shrimp (Penaeus monodon) (Tassanakajon et al., 1998), shrimp populations (Yoon and Kim, 2003) and deep sea lobster (Puerulus sewelli) (Park et al., 2005). The hierarchical dendrogram point out three main branches: cluster 1 (ATLANTIC 01 ~ ATLANTIC 07), cluster 2 (GUNSAN 08 ~ GUNSAN 14) and cluster 3 (CHINESE 15 ~ CHINESE 21). The shortest genetic distance displaying significant molecular difference was between individuals’ CHINESE no. 16 and CHINESE no. 18 (0.045). In the long run, individual no. 01 of the AHP population was most distantly related to CHINESE no. 19 (genetic distance = 0.430). The genetic distance between the Indian Ocean lobster and the Korean Slipper lobster species ranged between 0.040 and 0.612 (Park et al., 2005). Consequently, PCR analysis generated on the genetic data displayed that the geographic AHP population was widely separated from CHP population. From what has been said above, the potential of genetic analysis to identify diagnostic markers for the identification of three hairtail populations has been demonstrated. Generally speaking, using a variety of arbitrary primers, PCR has been applied to identify polymorphic/specific markers particular to line, species and geographical population, as well as genetic diversity/polymorphism in diverse species of organisms (McCormack et al. 2000; Yoon and Kim 2004).

PCR analysis generated on the genetic data showed that the geographic hairtail (Trichiurus lepturus) population from Korea in the Yellow Sea was more or less separated from geographic hairtail population from China in the South Sea. The average bandsharing value (mean±SD) within hairtail population from Korea showed 0.859±0.031, whereas 0.752±0.039 within population from China. Also, bandsharing values between two hairtail populations ranged from 0.470 to 0.611, with an average of 0.542±0.059. As compared separately, the bandsharing values of individuals within hairtail population from Korea were comparatively higher than those of individuals within population from China. The hierarchical dendrogram resulted from reliable oligonucleotides primers, indicating two genetic clusters composed of cluster 1 (KOREANHAIR1~KOREANHAIR11) and cluster 2 (CHINESEHAI12~CHINESEHAI22). The genetic distances between two geographic populations ranged from 0.038 to 0.476. Individual No. 11 within hairtail population from Korea was genetically closely related with No. 10 (genetic distance=0.038). The longest genetic distance (0.476) displaying significant molecular difference was also between individual No. 01 within hairtail population from Korea and No. 22 from Chinese. In the present study, PCR analysis has revealed significant genetic distances between two hairtail population pairs (P<0.05).

The turtle leg (Pollicipes mitella), which belongs to the family Pollicipes, is widely distributed in the rock along the shore areas of the southern sea, Jeju island and Ulleungdo island of Korean Peninsula. Generally, the size, type, stripe pattern, and color of this species vary in accordance with water depth, turbidity, nutrition, growth period, water temperature, and other environmental aspects. Genomic DNAs were isolated from 21 individuals of three turtle leg (P. mitella) populations of Tongyeong, Yeosu and Manjaedo located in the southern sea of the Korean peninsula. After incubation, we added 300 l of 3 M NaCl, and gently pipetted for a few minutes. 600 l of chloroform was then added to the mixture and inverted (no phenol). The DNA pellets were then incubation-dried for more than 10 hours, maintained at -40℃ until analysis, then dissolved in the ultra-pure water. The concentration of the extracted genomic DNA was measured by its absorbance ratio at 260 nm, with a spectrophotometer (Beckman DU 600 series, UK). Seven primers were exposed to generate the unique loci to each population and number of shared loci by the three populations of turtle leg which could be clearly scored. The hierarchical clustering tree was analyzed by the similarity matrices to generate a dendrogram using pc-package program Systat version 10. As regards average bandsharing value (BS) results, the higher fragment sizes (>1,200 bp) are much more observed in the Yeosu population. The number of unique loci to each population and number of shared loci by the three populations generated by PCR using 7 decamer primers in the turtle leg (P. mitella) population of Tongyeong, Yeosu and Manjaedo. Genetic distances among different individuals of the Tongyeong population of the turtle leg (lane 01~07), Yeosu population of the turtle leg (lane 08~14) and Manjaedo population of the turtle leg (lane 15~21), respectively, were generated using the CLASSIFICATION option in Systat version 10 according to the bandsharing values and similarity matrix. Tongyeong population could be evidently discriminated with the other two Yeosu and Manjaedo populations among three populations. The longest genetic distance (0.305) was found to exist between individuals' no. 02 of the Tongyeong population and no. 13 of the Yeosu population. It seems to the author that this is a result of a high degree of inbreeding in narrow region for a long while. Three turtle leg (P. mitella) populations can be clearly distinguished, especially, by their morphological characters and PCR-based approach.

The gDNA isolated from Cyclina sinensis from Gochang (GOCHANG), Incheon (INCHEON) and a Chinese site (CHINESE), were amplified by PCR. Here, the seven oligonucleotide decamer primers (BION-66, BION-68, BION-72, BION-73, BION-74, BION-76, and BION-80) were used to generate the unique shared loci to each population and shared loci by the three cyclina clam populations. As regards multiple comparisons of average bandsharing value results, cyclina clam population from Chinese (0.763) exhibited higher bandsharing values than did clam from Incheon (0.681). In this study, the dendrogram obtained by the seven decamer primers indicates three genetic clusters: cluster 1 (GOCHANG 01~GOCHANG 07), cluster 2 (INCHEON 08~INCHEON 14), cluster 3 (CHINESE 15~CHINESE 21). The shortest genetic distance that displayed significant molecular differences was between individuals 15 and 17 from the Chinese cyclina clam (0.049), while the longest genetic distance among the twenty-one cyclina clams that displayed significant molecular differences was between individuals GOCHANG no. 03 and INCHEON no. 12 (0.575). Individuals of Incheon cyclina clam population was somewhat closely related to that of Chinese cyclina clam population. In conclusion, our PCR analysis revealed a significant genetic distance among the three cyclina clam populations.

Hairtail, Trichiurus lepturus, ecologically warm water fish species, belonging to family Trichiuridae, widely distributed on the coast of the West Sea, South Sea and Jeju island in the Korean Peninsula and the several sea areas in China under the natural ecosystem. The food consumption of this species has increased considerably in various types of restaurants (including restaurants specializing in serving hairtails roasted and soy-sauce glazed cutlass fish with radish or other vegetables) for a long time. However, in spite of their economic and scientific consequences, a little information currently exist regarding the physiological and ecological levels only of hairtail species in Korea. Only the biological fisheries feature, distribution and migration of hairtail, Trichiurus lepturus, in Korean waters were in vestigated (Kimatal, 1998; Parketal, 2002). Lately, imported hairtail have been changed into endemic hairtail because of high margin. In the present study, to elucidate the genetic distances and differences among geographical hairtail, Trichiurus lepturus, populations, we performed a clustering analysis of two hairtail populations collected from Korea and China. Muscle tissues were collected separately from hairtail, Trichiurus lepturus, in dividuals from Korea in the Yellow Sea and China in the East Sea, respectively. RAPD-PCR analysis was performed on DNA samples extracted from a total of 44 (specimen numbers for duplicate experiments) individuals using eight arbitrarily selected primers of two decades of different decamer primers. Hairtail muscle was collected in sterile tubes, immediately placed on dry ice, and stored at －40℃ until the genomic DNA extraction. Genomic DNA was extracted and purified under the conditions described previously (Yoon and Kim, 2004). The degree of variability was calculated by use of the Dice coefficient (F), which is given by the formula: F = 2 nab/(na+nb), where nab is the number of bands shared between the samples a and b, na is the total number of bands for sample a and nb is the total number of bands for sample b (Jeffreys and Morton, 1987; Yoke-Kqueen and Radu, 2006). Euclidean genetic distances within- and between-populations were also calculated using the hierarchical dendrogram program Systat ver.10 (SPSS Inc., Chicago, IL, USA). The random primers OPA-07, OPA-20, OPB-14, OPB-15, OPB-17, OPB-18, OPD-16 and URP-07 showed common, polymorphic and specific bands produced using each primer to amplify genomic DNA isolated from the muscle of individuals. Accordingly, PCR analysis generated on the RAPD data showed that the geographic hairtail population from Korea in the West Sea was more or less separated from geographic hairtail population from China in the South Sea. The hierarchical dendrogram resulted from reliable four primers, indicating three genetic clusters composed of group 1 (Korean No. 1～11) and group 2 (Chinese No. 12～22). The genetic distances between two geographic populations ranged from 0.038 to 0.476. Individual No. 09 within hairtail population from Korea was genetically closely related with No. 06 (genetic distance = 0.104). The longest genetic distance (0.476) displaying significant molecular difference was also between individual No. 01 within hairtail population from Korea and No. 22 from Chinese. In the present study, RAPD-PCR analysis has revealed significant genetic distances between two hairtail population pairs. High levels of genetic polymorphisms and the existence of population differentiation between two hairtail populations showed RAPD-PCR approach is one of the suitable tools for individuals and/or population biological DNA studies.

동아시아에 분포하는 콩과에 속하는 칡 1종의 13집단과 근연분류군 2종의 4집단 등 총 17개의 개체군에 대하여 유전적 유연관계 및 종간 특이적인 분류학적으로 유용한 분자마커를 알아보기 위해 RAPD 분석을 실시하였다. 15개의 oligo primer를 이용한 효소중합반응을 통해 증폭된 RAPD 절편들은 200 bp에서 2,800 bp 사이의 구간에서 관찰되었다. 이중 유효한 polymorphic band makers는 총 208개, monomorphic bands는 3개를 확인하였으며, 칡의 종 특이적 분자마커는 4개로 확인되었다. 이러한 자료에 근거하여 칡속의 17개 개체군 집단에 대한 UPGMA 분석을 실시하였다. 도출된 UPGMA phenogram에서 한국산 칡 9개체군과 국외산 칡 3개체군이 각각 독립적인 두 개의 작은 유집군을 형성하였으며, 이후 두 유집군이 하나로 크게 유집되어 다른 칡 근연분류군과는 뚜렷하게 구분되었다. 따라서 RAPD 분석은 칡과 근연분류군간의 유연관계 분석 및 한국산과 국외산 집단의 원산지판별에 매우 유용한 분자마커로 생각된다.

갈대속 식물 두 종 달뿌리풀과 갈대의 종간 유연관계분석 및 수환경변화에 따른 종내 지역별 유연관계를 조사하기위해 RAPD 분석을 수행하였다. 총 9개의 oligoprimer를 이용한 효소중합반응에서 300 bp에서 1,900 bp 사이의 구간에서 142개의 유효한 polymorphic band를 확인하였다. Nei-Li의 genetic distance를 이용한 분석결과에 의하면, 비유사도지수가 달뿌리풀 개체군 종내에서는 0.012에서 0.061, 갈대 개체군 종내에서는 0.033에서 0.095의 낮은 상이성을 나타내어 종내 지역집단간에는 높은 유전적 유연관계를 보였으며, 달뿌리풀 개체군과 갈대 개체군 종간에서는 0.043에서 0.132의 상이성을 나타내어 달뿌리풀과 갈대 사이의 비유사도지수의 큰 차이는 보이지 않았으나 유전적으로 거리가 있는 것으로 나타났다. RAPD 결과 이 두 종 사이에 뚜렷한 차이를 구별할 수 있는 genetic marker를 확인하였고, UPGMA 유집분석에 의하면 두 종은 비슷한 수환경끼리 유연관계가 가까운 것으로 나타났고 다른 수환경끼리는 유연관계가 먼 것으로 나타났다. RAPD 분석법은 갈대속 두 종의 분류학적 혼동 해결과 동시에, 수환경변화에 따른 종내 지역별 유전적 유연관계 확인에 매우 유용한 방법인 것으로 나타났다.

고마리의 24개 지역집단으로부터 PCR을 통한 RAPD 분석을 실시하였다. PCR을 통해 증폭된 RAPD절편들은 200-1, 900bp 사이의 구간에서 관찰되었다. 총 16개의 oligoprimer를 이용한 효소중합반응에서 184개의 유효한 polymorphic band markers를 확인하였다. RAPD 분석결과를 기초로 UPGMA 방법에 의한 유집분석을 수행한 결과, 고마리 개체군은 교란형 하천(도시하천, 농촌하천)과 자연적 수환경으로 유집되었고, 교란형 하천 보다 자연적 수환경의 고마리 개체군끼리 유전적 유연관계가 높은 것으로 나타났다. 또한 자연적 수환경과 교란형 하천에 생육하는 고마리 개체군간에 뚜렷한 유전적 한계를 나타내어 이들 사이의 유전적 이질성이 있는 것으로 생각된다.

한반도의 동쪽에 위치해 있는 삼척(venus clam from Samcheok; VCS)과 원산(venus clam from Wonsan; VCW) 지역에서 채취된 민들조개(Gomphina aequilatera)에서 genomic DNAs(gDNAs)를 분리 추출하였다. 증폭산물은 primer agarose 전기영동법에 의해서 생성되었고, EtBr에 의해서 염색된 이후에 자외선에 의해서 확인되었다. 150 bp에서 2,400 bp에 해당되는 shared

참게(Eriocheir sinensis)와 꽃게(Portunus trituberculatus)의 2종으로부터 genomic DNA를 분리 추출하였다. 선택된 7개의 OPA-05, OPA-13, OPA-16, OPB-06, OPB-15, OPB-17 and OPD-10의 RAPD primer를 이용하여 identical, polymorphic 그리고 specific fragment를 얻어냈다. 본 연구에서 부안산 참게 집단에서는 505개의 fragment

RAPD analysis was performed to discuss the taxonomic status and phylogenetic relationships among the tribe Forsythieae and related groups. Two hundred and eighteen scorable polymorphic bands were detected from fourteen oligonucleotide primers. From the results of RAPD analysis by Nei and Li's genetic distance, each individuals of Abeliophyllum distichum showed high genetic relationships with ranging from 0.085 to 0.301, also the genus Forsythia showed from 0.042 to 0.655 among the species and populations. But, Abeliophyllum and Forsythia showed distinct dissimilarity, ranging from 0.610 to 1.258. And genetic differences among the population of Forsythia were 0.042 in F. koreana, 0.275 in F. saxatilis, 0.275 in F. ovata, 0.279 in F. nakaii, and 0.249 in F. viridissima. The UPGMA phenogram of tribe Forsythieae based on the results of RAPD analysis were presented that Abeliophyllum is distinct genus different from Forsythia. NJ tree which applied as the outgroups Fontanesia and Jasminum was derived, and it showed that tribe Forsythieae might be a monophyletic group. The genus Fontanesia was showed as sister group of tribe Forsythieae. Among the populations of taxa in Forsythia, F. koreana and F. saxatilis were more closely related, and F. ovata and F. nakaii were very closely related to F.japonica. And Fontanesia was the sister group of tribe Forsythieae.