The production of therapeutic protein from transgenic domestic animal is the major technology of biotechnology. Insulin-like growth factor-1 (IGF-1) is known to play an important role in the growth of the animal. The objective of this study is construction of knock-in vector that bovine IGF-1 gene is inserted into the exon 7 locus of β-casein gene and expressed using the gene regulatory DNA sequence of bovine β-casein gene. The knock-in vector consists of 5’ arm region (1.02 kb), bIGF-1 cDNA, CMV-EGFP, and 3’ arm region (1.81 kb). To express bIGF-1 gene as transgene, the F2A sequence was fused to the 5’ terminal of bIGF-1 gene and inserted into exon 7 of the β-casein gene. As a result, the knock-in vector is confirmed that the amino acids are synthesized without termination from the β-casein exon 7 region to the bIGF-1 gene by DNA sequence. These knock-in vectors may help to create transgenic dairy cattle expressing bovine bIGF-1 protein in the mammary gland via the expression system of the bovine β-casein gene.

Recently, with the increase of meat production, high quality and safety of meat have been strongly emphasized by Korean consumers. Marbling in beef has been regarded as an important criterion deciding meat quality in Korea. The purpose of this study was to identify the transcriptional level of insulin-like growth factor-1 (IGF-1) in longissimus muscle samples of 46 Hanwoo. The level of IGF-1 transcripts was measured by real-time polymerase chain reaction (PCR) and molecular connection of IGF-1 was analyzed using the Pathway Studio program (Ver 9.0). Increase of marbling score (MS) induced increase of IGF-1 transcripts level in the muscle and there is a significant correlation (p<0.05) between IGF-1 mRNA expression and MS. The pathway study showed that IGF-1 genes are regulated in insulin, fatty acid synthase, leptin, and corticotrophin releasing hormone. These results suggest that IGF-1 might be used as a useful marker for the improvement of economic traits in Hanwoo.

본 연구는 돼지 체외수정란의 배양시스템의 최적화를 위하여 수행되었다. 기본배양액으로는 NC-SU-23을 사용하였으며, 실험 1에서는 IGF-I이 각각 0, 1, 10, 100 ng/의 농도로 첨가된 배양액에서 배양하여 분할율, 배반포 발달율과총 세포수를 확인하였다. 농도 경사에 따른 분할율은 차이가 없었으나(65.5%~69.5%), 배반포 발달율에 있어서는 10 ng/의 농도로 첨가하였을 경우가 가장 높았다(P<0.05). 실험 2는 배 아 유전자 활성

본 연구는 성장인자 EGF, IGF-1, EGF+IGF-1 이 소 난포란의 체외성숙에 미치는 영향을 규명하기 위하여 실시하였다. 소 난포란의 체외성숙 시 EGF를 농도별 (10, 50 및 100ng/m1)로 첨가하여 실험한 결과 통계학적 유의성은 인정되지 않았으나 24시간 배양 후 성숙율은 대조군에 비하여 높은 경향을 나타내었다. 소난포란의 체외성숙 시 EGF와 IGF-1을 병용 처리하여 실험한 결과, EGF 또는 IGF-1 단독처리 군에 비하여 병용처

Background : Insulin-like growth factor 1 (IGF-1) appears to enhance the differentiation of osteoblasts and to activate the mineralization of bone. Hence, the aim of this study was to investigate the effects of ginseng complex on the remodeling of rats tibia. Methods and Results : Ginseng complex significantly increased serum IGF-1 by 58% and 34.5% than the control, respectively. Treatment with α-amylase when manufacturing these extracts remarkably increased the concentration of IGF-1 by 63% and 36% above the control, respectively. This ways that this ginseng complex, especially α-amylase treated extracts, contained a higher level of IGF-1 secretion in the ginseng complex groups. In addition, increases of 8% in femuf length were found after 12 weeks of oral administration with ginseng complex (300mg/kg). Conclusion : These results mean that ginseng complex have beneficial effects on bone effects on bone growth via IGF-1.