간행물
한국응용곤충학회 학술대회논문집
- 발행기관 한국응용곤충학회
- 자료유형 학술대회
- 간기 반년간
- 수록기간 2000 ~ 2024
- 주제분류 농수해양 > 농학 농수해양 분류의 다른 간행물
- 십진분류KDC 495DDC 595
권호리스트/논문검색
2009년도 한국응용곤충학회 춘계학술발표회 (2009년 5월) 209건
201.
2009.05
구독 인증기관·개인회원 무료
Kyong Sup Yoon, Deok Ho Kwon, Joseph P. Strycharz, Craig S. Hollingsworth, J. Marshall Clark, Si Hyeock Lee
The present investigation establishes deltamethrin resistance in the common bed bug, Cimex lectularius, populationcollected from New York City (NY-BB). The mortality resistance ratio indicated that NY-BB population was 264-fold more resistant to 1% deltamethrin in contact bioassay compared to one insecticide- susceptible population collected in Florida (FL-BB). Specific enzyme activities (general esterase, glutathione S-transferase, and 7-ethoxycoumarin O-deethylase) of NY-BB were not statistically different from those of FL-BB, indicating that the metabolic factors are not associated with the deltamethrin resistance in NY-BB. Complementary DNA fragments that encoded the open reading frame of voltage-sensitive sodium channel α-subunit genes from the FL-BB and NY-BB were obtained by homology probing PCR and sequenced. Sequence alignment of the internal and 5’ and 3’ RACE fragments generated a 6500 bp cDNA sequence contig, which was composed of a 6084 bp open reading frame (ORF) encoding 2027 amino acid residues and 186 bp 5’ and 230 bp 3’ untranslated regions (5’ and 3’UTRs, respectively). Sequence comparisons of the complete open reading frames of the sodium channel genes identified two point mutations (V419L and L925I) that were presented only in the NY-BB population. L925I, located the intracellular loop between IIS4 and IIS5, has been previously found in the pyrethroid-resistant populations of whitefly (Bemisia tabaci) that was more than 100-fold resistant to fenpropathrin. V419L, located in the IS6 transmembrane segment, is a novel mutation. This evidence suggests that the two mutations are likely the major resistance-causing mutations in the deltamethrin-resistant NY-BB via a knockdown-type nerve insensitivity mechanism.
202.
2009.05
구독 인증기관·개인회원 무료
Bo Yeon Kim, Kwang Sik Lee, Young Moo Choo, Hyung Joo Yoon, Pil Don Kang, Soo Dong Woo, Hung Dae Sohn, Jong Yul Roh, Yeon Ho Je, Byung Rae Jin
Background: Proteolytic enzymes are involved in insect molting and metamorphosis and play a vital role in the programmed cell death of obsolete organs. Here we show the expression profile of cathepsin B in the fat body of the silkworm Bombyx mori during development. We also compared the expression profile of B. mori cathepsins B (BmCatB) and D (BmCatD) in the fat body during the larval-pupal transformation of B. mori in the BmCatB or BmCatD RNA interference (RNAi) process. Results: BmCatB is ecdysone-induced and expressed in the fat body of B. mori during the molting, and the larval-pupal and pupal-adult transformations, and its expression leads to programmed cell death. In particular, BmCatB is highly expressed in the fat body of B. mori during the larval-pupal transformation and BmCatB RNAi treatment resulted in the arrest of the larval-pupal transformation. RNAi-treated BmCatB knock-down sustained the expression of BmCatD during the larval-pupal transformation. On the other hand, BmCatD RNAi up-regulated the expression of BmCatB in the fat body of final instar larvae. Conclusion: Based on these results, we conclude that BmCatB is involved in the programmed cell death of the fat body during B. mori metamorphosis and that BmCatB and BmCatD contribute collaboratively to B. mori metamorphosis
203.
2009.05
구독 인증기관·개인회원 무료
We describe here the cloning and characterization of a cDNA encoding the ferritin heavy chain homologue (TeFerHCH) from the cricket Teleogryllus emma. The TeFerHCH gene spans 1,009 bp and consisted of four introns and five exons coding for 217 amino acids residues. The TeFerHCH subunit contained the conserved motifs for the ferroxidase center typical of vertebrate ferritin heavy chains and the iron-responsive element (IRE) sequence with a predicted stem-loop structure was present in the 5'-untranslated region (UTR) of TeFerHCH mRNA. TeFerHCH was grouped with the S type (HCH) in a phylogenetic tree. The TeFerHCH cDNA was expressed as approximately 27 kDa polypeptide in baculovirus-infected insect Sf9 cells. Northern blot analysis revealed that TeFerHCH exhibited ubiquitous expression and was upregulated by wounding and iron overload in the fatbody, suggesting a functional role for TeFerHCH in iron metabolism.
204.
2009.05
구독 인증기관·개인회원 무료
Ming Shun Li, Jong Yul Roh, Xueying Tao, Zi Niu Yu, Zi Duo Liu, Qin Liu, Hong Guang Xu, Hee Jin Shim, Yang-Su Kim, Yong Wang, Jae Young Choi, Yeon Ho Je
Bacillus thuringiensis, an entomopathogenic bacterium belonging to the B. cereus group, harbors numerous extra-chromosomal DNA molecules whose sizes range from 2 to 250 kb. In this study, we used a plasmid capture system (PCS) to clone three small plasmids from B. thuringiensis subsp. kurstaki K1 using PCS which were not found in B. thuringiensis subsp. kurstaki HD-1, and determined the complete nucleotide sequence of plasmid pK1S-1 (5.5 kb). Of the six putative open reading frames (ORF2-ORF7) in pK1S-1, ORF2 (MobK1) showed approximately 90% aa identity with the Mob-proteins of pGI2 and pTX14-2, which are rolling circle replicating group VII (RCR group VII) plasmids from B. thuringiensis. In addition, a putative origin of transfer (oriT) showed 95.8% identity with those of pGI2 and pTX14-2. ORF3 (RepK1) showed relatively low aa identity (17.8-25.2%) with the Rep protein coded by RCR plasmids, however. The putative double-strand origin of replication (dso) and single-strand origin of replication (sso) of pK1S-1 exhibited approximately 70% and 64% identities with those of pGI2 and pTX14-2. ORF6 and 7 showed greater than 50% similarities with alkaline serine protease, which belongs to the subtilase family. The other 2 ORFs were identified as hypothetical proteins. To determine the replicon of pK1S-1, seven subclones were contructed in the B. t huringiensis ori-negative pHT1K vector and were electroporated into a plasmid cured B. thuringiensis strain. The 1.6 kb region that included the putative ORF3 (Rep1K), dso and ORF4, exhibited replication ability. These findings identified pK1S-1 as a new RCR group VII plasmid, and determined its replication region.
205.
2009.05
구독 인증기관·개인회원 무료
Seong-Ryul Kim, Mee-Yeon Hong, Kwang-Ho Choi, Min-Uk Kang, Tae-Won Goo, Seok-Woo Kang, Kwang-Gill Lee, Iksoo Kim, Jae Sam Hwang
Cecropin is an antimicrobial peptide that is synthesized in fat body cells and hemocytes of insect in response to a hypodermic injury or bacterial infection. A 503 bp cDNA encoding a cecropin-like antimicrobial peptide was isolated by employing annealing control primer (ACP)-based differential display PCR and 5'-RACE from immunized Papilio xuthus larvae. The open reading frame (ORF) of isolated cDNA encoded a 63 amino acid prepropeptide with a putative 22-residue signal peptide, a 3-residue propeptide and a 38-residue mature peptide with a theoretical mass of 4060.89 Da. The deduced amino acid sequence of peptide showed significant identities with other Lepidopteran cecropins. This peptide was named as papiliocin. RT-PCR revealed that the papiliocin transcript was detected at significant level after injection with bacterial lipopolysaccharide (LPS). Based on the deduced amino acid sequence of papiliocin, a 38-mer mature peptide was chemically synthesized by Fmoc method, and analyzed antimicrobial activity. The synthetic papiliocin peptide had a broad spectrum of activity against fungi, Gram-positive and negative bacteria, and also showed no hemolytic activity against human red blood cell.
206.
2009.05
구독 인증기관·개인회원 무료
잣종실해충의 피해가 심한 남양주시 수동면의 잣나무임지를 대상으로 잣나 무의 해충종류를 파악하기 위하여 2007년부터 2008년까지 조사기간은 5월부 터 9월, 시간은 8시부터 10시까지 주 1회 유아등을 이용한 야간채집조사로 나 비목 곤충류를 채집 조사한 결과 2007년에는 21과 261종, 2008년에는 28과 351종으로 총 28과 353종의 나비목 곤충류가 조사되었다. 그 중 밤나방과가 91종, 자나방과가 65종으로 가장 많은종이 조사되었으며, 잣 종실해충으로 잘 알려진 솔알락명나방 등의 명나방과가 39종, 백송애기잎 말이나방 등의 잎말이나방과가 27종의 순으로 많은종이 조사되었다. 이러한 결과로 기존에 알려진 주요 종실해충이 속해있는 명나방과와 잎말 이나방과 외에도 잣 종실을 가해하여 경제적 피해를 줄 수 있는 나비목 종류 가 상당히 존재하리라 판단된다. 따라서 앞으로 새로운 종실해충을 찾아내고 그들의 발생생태 및 방제체계를 구명함으로서 잣 종실해충의 피해를 줄인다 면 농가소득에 커다란 보탬이 될 수 있을 것으로 기대한다.
207.
2009.05
구독 인증기관·개인회원 무료
Min-Uk Kang, Kwang-Ho Choi, Tae-Won Goo, Seong-Ryul Kim, Seung-Won Park, Seok-Woo Kang, Kwang-Gill Lee, Si-Kab Nho
For the purposes of this paper, stress may be defined as any modification of environmental parameters that leads to a response by biological organisms. Stresses that affect biological structures may be non-thermal, such as ultraviolet radiation, pH, or salinity, or thermal. Temperature is one of the major stresses that all living organism face. The major effects of cold shock are decrease of membrane fluidity and the stabilization of secondary structures of RNA and DNA in the cells, which may effect the efficiency of translation, transcription, and DNA replication. In this study in compliance with the cold temperature stress about selection of the useful gene is contents from the silkworm which is been revealed. The survival rate which is caused by with the cold temperature stress until 12 hours was 100% in 0℃, until 2 hours was 100% in -5℃. A total of 960 clones were randomly selected from the subtraction cDNA library, and then performed a differential display hybridization analysis with forward and reverse probes. In conclusion, selected 53 partial clones and novel 2 full-length clones were identified as differential expressed genes. We assumed that the novel gene related with transmembrane.
208.
2009.05
구독 인증기관·개인회원 무료
The susceptibility of the Blattella germcanica (L.) in the Republic of Korea (ROK) to insecticides was evaluated under laboratory conditions using 12 insecticides currently used by the local public health centers and/or pest control operators in the ROK. The insecticides included seven pyrethroids and five organophosphages. Based on their LD50values, the order of susceptibility of B. germanica adults to the insecticides was chlorpyrifos-methyl, profenofos, and chlorpyrifos with the values of 0.07, 0.29, and 0.88 ㎍/♀, respectively. The least susceptibility was obtained with tetramethrin at LD50 of 7.39 ㎍/♀. In the comparative resistance test, the resistance ratios (RR) of 12 insecticides were compared to each other using field collected B. germanica adults in Seoul between 1993 and 2007. Blattella germanica demonstrated higher RRs to pyrethroids such as λ-cyhalothrin, and low RRs among the organophosphates. Among the pyrethroids, λ-cyhalothrin had the highest RRs of 111 and 129 fold differences at LD50 and LD90 values, respectively. Among the organophosphates, profenofos was observed to have the highest RRs of 4 and 15 fold differences at LD50 and LD90 values, respectively. However, there were no significant differences in susceptibility to tetramethrin, chlorpyrifos, and fenitrothion. Blattella germanica was more susceptible to pyridafenthion showing a 0.7 fold difference in a resistance rate (RRLD50), respectively. Resistance ratio of tetramethrin was low, but susceptibility was also not high.
209.
2009.05
구독 인증기관·개인회원 무료
우리나라 벼 재배지에서는 친환경적 잡초관리를 위해 주로 왕우렁이, 쌀겨 및 오리를 이용하고 있다. 이런 유기농업은 화학농약 40% 감축목표 및 농촌 고령화에 따른 일손부족 등 사회경제적 이유로 인해 해마다 재배면적이 증가 되고 있으나 한편으론 병해충 및 잡초의 발생도 더불어 증가하고 있다. 경남 창녕지역은 최근 따오기 복원 사업 등 우포 생태공원 조성에 따른 벼 유기농업면적이 ‘08년 420ha까지 크게 증가하였는데, 이 지역의 주 유기농법 인 쌀겨 이용 벼 재배지에 최근 자귀풀 발생량 증가와 주로 콩에 발생하는 가로줄노린재 등이 많이 발생하여 주변 벼에도 피해를 주고 있다. 창녕군내 쌀겨 및 왕우렁이 이용 벼 유기농업지에 발생하는 주요 잡초는 피, 올방개, 가막사리, 여뀌바늘, 자귀풀 등이었다. 벼 수확기 전후에 발생된 잡초 우점종 은 올방개 및 피로 각각 m2당 13.0 및 6.0본이었고, 자귀풀은 m2당 0.3본으로 발생량은 적었다. 쌀겨이용 벼 유기농업 재배단지인 창녕 우포 및 신기 두 지 역간 노린재 발생량은 9월 상순까지는 자귀풀보다 콩에서 발생량이 더 많았 으나 9월 하순에는 자귀풀에서 노린재 발생량이 더 많았다. 특히 9월 하순 우 포 및 신기지역 자귀풀 10주당 노린재 발생량은 우포가 889마리, 신기 131마 리로 우포가 월등히 많았고, 노린재에 의한 자귀풀 주변 벼의 반점미율도 우 포 및 신기에서 각각 12.7%, 4.3%로 우포에서 더 높았다. 자귀풀에 발생된 노 린재 종류는 가로줄노린재, 풀색노린재, 톱다리개미허리노린재였고, 가장 우점 종은 가로줄노린재였다.
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