Understanding how researchers are tackling globally important issues, such as climate change, is crucial to identify whether current research is comprehensive enough to make substantive predictions about general responses to climate change. We assessed the type of studies being conducted by researchers to understand the impacts of climate change on insects, published. Most published research is generated from Europe and North America and being dedicated to core data analysis, with reviews being highly produced. Temperature – only is the main climate change factor being analysed, with most researchers are assessing changes in abundance or distribution/range shifts. Of most concern is the number of studies which do not specifically identify a climate change factor (ie just arm wave), the lack of studies on Hemimetabolous insects and the need for more studies to assess specific mechanistic responses to climate change.

톱다리개미허리노린재(Riptortus pedestris [Fabricius] [Hemiptera: Alydidae])는 콩에서 특히 중요한 해충이다. 효과적 관리 전략 수립을 위해 톱다리개미허리노린재의 계절적 발생 패턴과 기주 식물의 먹이 적합성, 천적 기생벌들의 발생 소장과 상호관계에 대해 연구한 결과를 요약하였다. 톱다리개미허리노린재는 보리, 참께, 콩 등이 복합적으로 재배되는 전작지에서 모두 채집되었으나 콩에서 가장 밀도가 높았다. 콩 외의 작물은 실내 먹이실험에서 성충으로 발육하지 못하여 콩이 가장 주요한 먹이원임을 알 수 있었다. 한편 노린재검정알벌(Gryon japonicum [Ashmead] [Hymenoptera: Scelionidae])과 노린재깡충좀벌(Ooencyrtus nezarae Ishii [Hymenoptera: Encyrtidae])이 톱다리개미허리노린재의 주요 천적이며, 노린재검정알벌은 봄에서 초여름에 노린재깡충좀벌은 여름과 가을에 우점을 보인다. 이는 두 종이 경쟁적 배제를 통한 한 종의 멸종이 일어나지 않게 기주 자원을 시간적으로 분할한 결과로 보인다. 이러한 노린재와 기주식물(bottom-up) 그리고 천적(top-down)과의 상호관계의 이해를 통한 해충관리전략에 대해 고찰하였다.

The 3-isobutyl-1-methylxanthine (IBMX) is non-selective phosphodiesterase and is able to prevent resumption of meiosis by maintaining elevated cyclic AMP (cAMP) concentrations in the oocyte. The present study was conducted to analyze: (1) nuclear maturation (examined by the Hoechst staining), (2) whether cytoplasmic maturation (examined by the intracellular glutathione (GSH) concentration) of porcine oocytes is improved during meiotic arrest after prematuration (22 h) with IBMX. Before in vitro maturation (IVM), oocytes were treated with 1 mM IBMX for 22 h. After 22 h of pre-maturation, the higher rate of IBMX treated group oocytes were arrested at the germinal vesicle (GV) stage (42.3%) than control IVM oocytes (10.1%). It appears that the effect of IBMX on the resumption of meiosis has shown clearly. In the end of IVM, the reversibility of the IBMX effect on the nuclear maturation has been corroborated in this study by the high proportions of MII stage oocytes (72.5%) reached after 44 h of IVM following the 22 h of inhibition. However, intracellular GSH concentrations were lower in the oocytes treated with IBMX than the control oocytes (6.78 and 12.94 pmol/oocyte, respectively). These results demonstrate that cytoplasmic maturation in porcine oocytes pre-treated with IBMX for 22 h did not equal that of control oocytes in the current IVM system. These results indicate that pre-maturation with IBMX for 22 h may not be beneficial in porcine IVM system.

Charles Stankievech’s 2011 installation LOVELAND includes a wall-sized screen depicting video footage of a barren arctic landscape in an enclose room, painted and bathed in white light, that appears as an extension of the imaged environment. A melodic and industrial musical score emanates from multiple sound panels and as the music increases a cloud of purple smoke becomes visible on the horizon line in the distance and gradually advances toward the viewer until it completely fills the screen. The smoke then remains, rushing about madly and lapping at the border between the screen and the room before it suddenly subsides and the spectator is again left with the desolate landscape. The entire process takes a mere five minutes and then, fixed on an endless loop, begins again. This paper positions LOVELAND as an attempt to simulate a sublime experience of the end of the world through a transposition of the Arctic atmosphere into the gallery space. Encompassing a discussion of the historical and contemporary significance of the Arctic in popular culture, aesthetics and environmental politics, it is suggested that Stankievech employs an apocalyptic trope in reference to the unstable position of the North in the current political and ecological climate. Revisiting critiques of modernist exhibition practices and investigating the perceptual and temporal dimensions of the work, this analysis focuses primarily on the experience of the installation’s spectator. Visually, aurally and phenomenologically immersed, the viewer is made subject to, and implicated in, the events unfolding on the screen and within the space. Due to the looping of the video footage, this paper argues that the apocalypse imaged in LOVELAND is presented as an endless event – incessantly enacted, yet infinitely deferred – and that the spectator is enveloped in an uncertain and unceasingly extended present moment.

본 연구는 반추동물의 조사료 자원으로서 거대억새를 개발하기 위한 목적으로 수행되었다. 우리나라에서 새롭게 개발된 품종인 거대억새 1호를 완숙기 이후에 채취하여 in vitro 반추위 발효를 이용해반추위내 pH, 암모니아태 질소, 가스발생량, 휘발성 지방산 생성량 및 건물소화율을 조사하였으며,볏짚과 비교하여 평가하였다. 거대억새는 볏짚에 비하여 유의적으로 높은 반추위내 pH를 나타내었다(p<0.01). 암모니아태 질소의 경우 배양 12시간 이후에는 두 처리구간의 유의적인 차이를 나타내지 않았다 (p>0.05). 배양 6시간 이후 부터는 거대억새의 가스발생량이 볏짚에 비하여 유의적으로 낮게 나타났다 (p<0.05). 휘발성 지방산 생성량에 있어 acetate, propionate, butyrate, valerate 및 총생상량에서 볏짚이 거대억새보다 높게 나타났다. 그러나 iso-butyrate와 iso-valerate에서는 두 조사료원별 차이는 발견되지 않았다. 건물소화율에 있어 배양 12~24시간 사이의 거대억새 소화율이 볏짚에 비하여 유의적으로 나타났다. 결론적으로 거대억새의 이용성은 볏짚의 약 80% 수준인 것으로 나타났다.

파나마의 농경지는 전체 국토면적 74,340 km2의 약 30%에 해당하는 22,300 km2이다. 파나마 전체인구 3,508천명의 약 25%에 해당하는 사람들이 농촌에 거주하고 있으며, 총 취업인구에서 농업부문이 차지하는 취업자 비중은 17.6%수준이다. 경제발전에 따라 전체 GDP에서 농업부문이 차지하는 비중은1995년 10.5%에서 2010년 4.6%로 감소하고 있으나 농식품수출이 파나마 전체 상품수출에서 차지하는 비중은 75%로 매우 높은 수준이다. 주요 수출 농산물은 멜론, 수박, 바나나, 파인애플 등 열대과일이다. 이들 주요 수출품이 전체 농산물 수출에서 차지하는 비중은 20%이상으로 높은 수준이며, 열대과일 수출의 90%이상이 유럽연합과 북아메리카지역의 소수 국가에 집중되어 수출되고 있다. 또한 파나마의 전략 수출 농산물인 열대과일은 주로 부가가치가 낮은 신선상태로 수출 중으로 연도별 수출도 안정적이지 못한 상황이다. 우선 아시아 신흥시장 등 다른 수출유망 지역과 국가로 수출선이 다변화되지않은 이유는 첫째, 긴 수송거리로 인한 운송비 등 유통비용문제, 둘째, 콜드체인시스템 등 저장유통기술과 표준화 부족으로 인한 고품질 유지의 어려움, 셋째, 달러가치의 상승으로 인한 수출경쟁력 저하, 넷째, 고부가가치 상품개발 미흡과 식품가공산업의 미발달 등이다. 파나마의 기후 등 농업환경을 고려할 때 열대과일은 생산력 증진과 지속적 품질 향상을 통해수출경쟁력 제고가 가능하고 수출산업화가 가능한 분야이다. 하지만 파나마가 이들 품목을 한국, 일본, 중국 등 동북아시아주요 수출시장에 수출하기 위해서는 무엇보다 해외시장 개척을 위한 더 많은 정책적 노력이 필요하다. 특히 파나마 정부가 성공적으로 파나마산 열대과일을 동북아시아 시장에 수출하기 위해서는 이들 대표적인 수출 품목을 안정적으로 해외시장에 수출할 수 있는 수출단지 육성을 적극적으로 고려하고,이들 품목과 단지를 중심으로 해외마케팅이나 수출지원 프로그램을 집중화해 나갈 필요가 있다.

유채 품종에서 황 공급수준이 황산염 흡수 및 동화에 미치는 영향을 구명하고자 어린 잎과 성엽조직에서 ATP sulfurylase (ATPs), SO42－ 흡수, 글루타치온 함량을 분석하였다. 본 실험에서 10가지 유채 품종들 (Mosa, Capitol, Saturnin, Akela, Pollen, Mokpo, Youngsan, Tamra Colosse 그리고 Naehan)은황 공급수준에 따라 몇 가지 황산염 흡수와 동화 능력이 다르게 나타났다. 황 결핍 조건에서 모든 품종의 ATP sulfurylase (ATPs) 활력은 늙은 잎에 비해 어린 잎에서 높게 나타났으며, 글루타치온 함량은황 공급수준이 감소함에 따라 어린 잎에서 많이 증가하였다. 이러한 결과들은 유채 품종별 황 결핍조건에서 황을 이용하는 능력이 다르다는 것을 잘 보여주었다.

Ski protein is a nuclear transcription factor that does not bind DNA directly. Due to its unique binding properties with multiple factors, Ski could perform various roles in the regulation of both cellular proliferation and differentiation. We had previously reported that Ski protein is present in granulosa cells of atretic follicles, but not in preovulatory follicles, suggesting that Ski has a role in apoptosis of granulosa cells. The alternative fate of granulosa cells other than apoptosis is to differentiate to luteal cells; however, it is unknown whether Ski is expressed and has a role in granulosa cells undergoing luteinization. Thus, the aim of the present study was to examine whether the initiation of luteinization with luteinizing hormone (LH) directly regulates expression of Ski in the luteinized granulosa and luteal cells after ovulation by in vitro models. RT-PCR and real time PCR analysis respectively revealed that LH had no effect on c-Ski mRNA expression in the cultured granulosa cells regardless of LH treatment. Though Ski protein isabsent in granulosa cells of preovulatory follicle, its mRNA(c-Ski) was expressed and the level was unchanged even after LH surge. Taken together, these results demonstrated that Ski protein expression is induced in granulosa cells upon luteinization, and suggested that its expression is regulated post-transcriptionally. Moreover, expression of mRNA of Arkadia, an E3 ubiquitin ligases, in luteinizing granulosa cells in vivo was assessed by real time-PCR. The levels of Arkadia mRNA expression were unchanged during follicular growth and post ovulatory luteinization. These findings suggest that Ski protein level may be regulated during luteinization at translational and/or post-translational level but not by Arkadia.

The coupling of autophagy and endoplasmic reticulum (ER) stress has been implicated in a variety of biological processes. However, little is known regarding the involvement of the autophagy/ER stress pathway in early embryogenesis or the underlying mechanism (s). Here, we showed that the developmental competence of in vitro-produced (IVP) bovine embryos was highly dependent on the autophagy/ER stress balance. Although relative abundances of autophagy-associated gene transcripts, including LC3, Atg5, and Atg7 transcripts, were high in oocytes and throughout the early stages of preattachment development, extensive autophagosome formation was only detected in fertilized embryos. Using inducer and inhibitor of autophagy, we showed that transient elevation of autophagic activity during early preattachment development greatly increased the blastocyst development rate, trophectoderm cell numbers, and blastomere survival; these same parameters were reduced by both inhibition and prolonged induction of autophagy. Interestingly, the induction of autophagy reduced ER stress and associated damage, while the developmental defects in autophagy-inhibited embryos were significantly alleviated by ER stress inhibitor treatment, indicating that autophagy is a negative regulator of ER stress inearly embryos. Collectively, these results suggest that early embryo genesis of IVP bovine embryos depends on an appropriate balance between autophagy and ER stress. These findings may increase our understanding of important early developmental events by providing compelling evidence concerning the tight association between autophagy and ER stress, and may contribute to the development of strategies for the production of IVP bovine blastocysts with high developmental competence.

Autophagy is known to be involved in a variety of biological processes. However, relatively a little is known regarding oocyte maturation and preimplantation development in mammals. Thus, the current study was conducted to investigate the role of autophagy in oocyte maturation and subsequent preimplantation development in pigs. Porcine oocytes were matured in the presence or absence of 1 μM rapamycin, an autophagy inducing agent, fertilized in vitro, and cultured to blastocyst stage. From Western blotting analysis, we found that active form LC3 was detected during in vitro maturation (IVM) period, suggesting the possible role of autophagy in oocyte maturation. Interestingly, treatment of rapamycin during IVM significantly increased nuclear maturation compared to control group. Importantly, rapamycin-assisted IVM greatly improved monospermic fertilization and blastocyst development rates compared to control embryos. In addition, we also found that cell number and blastomere survival in blastocysts were markedly increased in rapamycin treatment group, which was further evidenced by both elevation of anti-apoptotic transcript Bcl-XL and decrease of pro-apoptotic transcript Bax. Collectively, these results strongly suggest that induction of autophagy may contribute to the completion of nuclear and cytoplasmic maturation of porcine oocytes.

Rhabditis blumi, isolated from Exomala orientalis, is a necromenic type of nematode that possesses several attributes of a potential biological control agent such as ease in culture conditions, high rates of reproduction and virulence, short life cycle and rapid multiplication etc. Pathogenicity of R. blumi was tested against 5 coleopteran species and 5 lepidopteran species. All insect larvae were susceptible to R. blumi, showing over 60% mortality except for Holotrichia kiotoensis and Ectinohoplia rufipes. The highest mortality was observed in Plutella xylostella (88%), followed by Artogeia rapae, Exomala orientalis, Spodoptera litura and Anomala rufocuprea (over 70%). Reproductive capacity of R. blumi differed from each insect species, ranging from 17 to 656 dauer juveniles/mg of insect larva. Dauer juvenile production was most in Plutella xylostella larvae, producing 656 dauer juveniles/mg of host body weight, followed by E. orientalis, H. kiotoensis, and A. rapae larvae (over 100 dauer juvenile per mg).

Ooencyrtus nezarae Ishii and Gryon japonicum (Ashmead), egg parasitoids of Riptortus pedestris (Fabricius), coexist despite direct competition for host eggs. As asymmetrical pattern of seasonal occurrence, i.e., more G. japonicum during spring-summer and more O. nezarae during summer-fall, has been reported, host resource partitioning may occur in temporal scales. To test this hypothesis, we demonstrated the interspecific competition between the two species by measuring parasitism in nine combinations of host densities and exposure times. To reflect gregarious-solitary dichotomy, three O. nezarae and one G. japonicum mated females were used in each experiment. O. nezarae was better competitor when exposure time was longer than 1 day irrespective of host densities. Parasitism rate and progeny emergence of O. nezarae was 1.6-2.8 and 4.7-7.3 times higher than G. japonicum. O. nezarae has higher potential rate of increase than G. japonicum due to gregariousness, and be more successful in larval competition inside multiparasitized host egg as it acts as a facultative hyperparasitoid. Although G. japonicum was more effective in host finding (as they showed relatively higher per capita parasitism and progeny emergence), their progeny suffered high mortality from the larval competition with O. nezarae. These results may explain the asymmetrical occurrence pattern in the field.

톱다리개미허리노린재(Riptortus pedestris [Fabricius] [Hemiptera: Alydidae])는 콩 포장에서 주요 해충이며, 기생천적으로는 노린재깡충좀벌(Ooencyrtus nezarae Ishii [Hymenoptera: Encyrtidae])과 노린재검정알벌(Gryon japonicum (Ashmead) [Hymenoptera: Scelionidae])이 있다. 저온 저장시켜 불활성화된 노린재 알을 집합페로몬 트랩에 부착하면 기생률이 증가한다고 보고되었다. 본 연구에서는 위 기술을 이용하여 불활성화된 노린재 알의 밀도에 따른 기생률 향상 효과를 조사하였다. 경북 의성군 옥산면에 위치한 콩 포장(100×140m)을 12개의 시험구로 나누고 집합페로몬 트랩을 각각 4개씩 설치하였다. 시험구에는 3개의 처리구와 1개의 대조구를 두었으며, 처리구에는 1, 2, 4개의 집합페로몬 트랩에 트랩당 60개의 알을 부착하였다. 그 결과 처리구에서 노린재깡충좀벌에 의한 기생률은 23~51%이었고, 대조구에서는 25%이었다. 노린재검정알벌의 경우 처리구에서 19~42%의 기생률을 보여 주었고, 대조구의 기생률은 22%이었다. 240개의 알을 부착한 시험구에서 노린재깡충좀벌과 노린재검정알벌에 의한 기생률이 최대 51, 42%이었고, 노린재 알을 설치하지 않은 대조구의 기생률 보다 각각 2.1, 1.9배가 높았지만 톱다리개허리노린재의 밀도는 차이가 없었다.

Aggregation pheromone of Riptortus pedestris (Fabricius) (Hemiptera: Alydidae) has kairomonal effect on its egg parasitoids. Thus, inclusion of non-viable host eggs of R. pedestris to aggregation pheromone trap was suggested to propagate parasitoids attracted in the trap. In this study, spatial and temporal distribution of egg parasitoids were investigated in sesame fields in Songcheon, Andong where aggregation pheromone traps were installed with non-viable host eggs. Parasitism changes were assessed after collecting non-viable host eggs released at distances of 8, 16, 24, and 32 m from the trap. Higher egg parasitism and adult emergence was found up to 16 m from the point of release. Parasitism increased over time, but decreased with distance. For the comparison of change in temporal parasitism, traps were installed without host eggs in another sesame field. Parasitism rate in sesame field without host eggs increased by 13%, whereas, in the field with host eggs, the parasitism increased up to 44%. These findings suggest that host eggs attached to the pheromone traps help the parasitoids propagate and disperse.