This study was carried out to investigate the difference of the content of soil chemical components and growthcharacteristics in six years old ginseng affected by application of decomposing plant residues in paddy-converted field. Theresults show that aerial parts of ginseng are no difference between press cake (PC) 200㎏/10a and control but subterraneanparts of ginseng PC 200㎏/10a, especially quantity related root fresh weight and tap root diameter, are statically about 1.6times heavier and about 1.2 times thicker than the ginseng control. Furthermore, the survival rate of PC 200㎏/10a is67.1% rise significantly compare with the control 50.7%. But compared with the PC 200㎏/10a and the PC 400㎏/10a,ginseng root growth and survival rate of PC 400㎏/10a get worse and that increase physiological disorder occurrence ratethan PC 200㎏/10a. Even though there are no significant differences between the ginseng of decomposing plant residuesexcept press cake treatment and the ginseng of control in growth characteristics, it does tend to increase the survival rateand decrease the physiological disorder occurrence rate in most fertilizer treatment except for RSC 2ton/10a, RSC 4ton/10a and RH 4kL/10a. Noted that EC is highly increased and exceeded 1.7ds/m in RSC 2ton/10a, RSC 4ton/10a and RH4kL/10a. It would be caused physiological disorder in many ways and affected ginseng growth characteristics, survival rate.

목초액을 친환경 인삼재배에 이용하기 위하여 전엽기 이후인 6월 중순부터 지상부에 엽면시비로 살포하여 3년생 인삼 잎의 형태적특성과 수량에 미치는 영향을 조사하였다. 목초액을 처리하면 처리농도에 관계없이 해면조직의 배열이 3층에서 4층으로 증가하였다. 또한 인삼 잎의 상표피와 하표피의 두께가 두꺼워지고, 엽육세포도 두꺼워지는 효과를 보였으며 지하부의 생육에도 영향을 미쳐 무처리에 비해 뿌리의 생육이 증진되었으며 인삼 뿌리의 수량도 증가하였다. 따라서 목초액의 혼합사용은 인삼의 생육을 촉진시키고, 병해를 극복하여 인삼을 친환경재배 할 수 있는 대안이 될 것이다.

Korean Panax ginseng C. A. Meyer (P. ginseng) is a well-known and one of the most important tonic herbs used in traditional Korean medicine. The pharmacological effects of P. ginseng have been reported by many researchers. Nevertheless, little is known between the mechanism of action and the active compounds. In this study, we performed a comprehensive proteomic analysis and protein categorization in order to understand the physiological characteristics of the major components in the adventitious roots of P. ginseng. Whole proteins extracted from the cultured adventitious roots of P. ginseng were separated by two-dimensional polyacrylamide gel electrophoresis (2-DE). Among the 1000 spots which were detected by silver staining, 113 spots were labeled and identified by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF MS). Our results showed that 40 proteins were identified among the 113 spots, with a hit ratio of 35.3%. A number of proteins identified on the 2-DE gels (30%; 16 spots) were involved in energy metabolism. These proteomic data will be helpful to better understand the physiological and pharmacological effects of P. ginseng.

To study the cause of physiological disorder in leaf of ginseng cultivated at paddy soil, the degree of brown-colored symptom (BCS) and the contents of inorganic matter in leaf were investigated by irrigating the solution of ferric andferrous iron of 0.1~2.0%, and citric acid of 1.0~4.0% on bed soil, respectively. Ratio of BCS by variety was as high as85.0% in Yoenpoong, while it was as low as 5.4%, 7.5% in Chunpoong and Hwangsook, respectively. The contents ofinorganic matter of leaf in Yoenpoong were lower in P₂O5, Ca, and Mg, while it were higher in K, Fe, and Mn than othervariety. Iron solution caused BCS more distinctly when each ferric and ferrous iron were dissolved with 1.0% citric acidthan when each iron was dissolved without citric acid. Ferric iron caused BCS more effectively than ferrous iron. BCSoccurred in 4.0% citric acid was as same as 2.0% ferric iron mixed with 1.0% citric acid. Low P₂O5 and high Fe content inleaf appeared in both of artificial and natural symptoms. We concluded that excessive Fe uptake caused BCS to leaf becausethe solubility of iron was increased in condition of low soil pH.

This study was carried out to investigate change of ginsenoside contents in red and fresh ginseng according toroot part and age by hydrolysis. Neutral total ginsenoside contents by hydrolysis in 6-year main root and lateralroot were significantly increased than those by non-hydrolysis, as 41.6 and 32.8%, respectively. However, there wasno significant difference in red ginseng. In fresh ginseng, ginsenoside contents of the protopanaxatriol group such as Re, Rf,Rg₁, Rg₂, and Rh₁ were not significantly different, but Rb₁, Rb₂, Rb₃, Rc, and Rd showed significant difference. The increaserate of neutral total ginsenoside content by hydrolysis was higher in epidermis-cortex than stele. Also, the neutraltotal ginsenoside content was fine root>rhizome>lateral root>main root, respectively. While there was no tendencytowards the increase of ginsenoside by hydrolysis with the increase of root age in fine root and rhizome, there was significantdecrease in main root and lateral root.

Ginsenosides of roots in Panax ginseng were analyzed by metabolic-targeting HPLC using the partial leastsquares discriminant analysis (PLS-DA) and compared depending on sowing methods between direct seeding andtransplanting method. Score plots derived from PLS-DA could identify the sowing method between the direct seeding andtransplanting method in P. ginseng roots. The ginsenoside compounds were assigned as Rg1, Re, Rf, Rg2, Rb1, Rc,Rb2, Rb3, and Rd. Contents of Re, Rf, Rg2, Rb1, Rc, Rb3, and Rd of main roots produced from the transplanting methodwere relatively higher than those of samples produced from direct seeding method. Also, contents of Rg1, Re, Rf, Rg2, Rb1,Rc, Rb2, Rb3, and Rd of lateral roots from the transplanted samples were relatively higher than those of samples producedfrom direct seeding method. Therefore, HPLC with PLS-DA analysis can be a straightforward tool for identificationof ginsenosides in main or lateral roots of P. ginseng obtained from two different seeding methods between directand transplanting methods.

‘천량’은 생리장해 저항성 품종 육성을 목적으로 1999년도 경기도 여주 농가 포장에서 수집해 2002년에 개체를 선발하 였고, 2003년부터 2006년까지 특성검정과 2007년도부터 2009년도까지 생산력검정시험을 실시하였다. ‘음성1호’의 계 통명을 부여한 후 2010년부터 2011년까지 지역적응시험을 실시한 결과, 다수성이면서 염류저항성이 강한 품종으로 인정 되었고, 2011년에 직무육성 신품종 선정위원회에서 신규 등 재 품종으로 선정되어 ‘천량’으로 명명하였다. ‘천량’의 잎색 은 녹색이며, 소엽 가로 자른면의 모양이 볼록형으로 대조품 종인 ‘천풍’의 오목형과 비교된다. 줄기의 색깔은 전체가 연 두색이나 엽병 주위와 기부에 연한 자색을 나타낸다. ‘천량’ 의 열매색과 잎노화색은 적색을 나타내나 대조품종에서 등황 색, 황색을 나타내어 확연히 구분이 되었다. ‘천량’은 ‘천풍’ 보다 출아기는 2일, 개화기는 3일 그리고 과육성숙기는 5일 빨랐다. 4년생에서 ‘천량’의 경장은 41.3 cm로 ‘천풍’에 비해 짧았으며, 경직경은 6.4 cm로 ‘천풍’에 비해 더 굵었고, 엽장, 엽폭, 엽병장도 ‘천풍’보다 더 길었다. ‘천량’의 근장과 동체 장은 대조품종과 비슷하며, 동직경은 대조품종보다 5.3 mm 굵었고, 주당 생근중은 57.0 g으로 ‘천풍’보다 더 무거웠다. ‘천량’의 주당 열매수는 ‘천풍’보다 더 적게 맺혔으나 천립중 은 53.1 g으로 ‘천풍’보다 무거웠고 개갑률은 ‘천풍’보다 5.4% 높게 나타나 우량 묘삼 생산에 유리할 것으로 생각되었 다. ‘천량’의 수량은 생산력검정을 한 결과 평균 수량이 536 kg/10 a로 ‘천풍’보다 12% 증수되었다. ‘천량’은 모잘록병과 탄저병에 대해서는 저항성을 나타내었으며, 점무늬병에서는 중도 저항성을 나타내었다. 역병과 뿌리썩음병은 거의 발생되 지 않았고 가루깍지벌레와 선충 등 충해에 대해서는 저항성 을 나타내었다. ‘천량’은 염류 과다로 발생되는 지상부의 황 증 현상과 지하부 적변삼 증상은 거의 나타나지 않았으며, 고 온에는 중도 저항성을 나타내어 천풍보다 강하게 나타났다. 인삼의 주요 성분인 ginsenoside 함량 중 total 함량은 22.18 mg/g으로 ‘천풍’과 비슷하게 나타났으며, 조사한 ginsenoside 8종류 중 Rb1과 Rf를 제외한 6종류의 함량은 ‘천량’이 높게 나타났다.

The development of random amplified polymorphic DNA (RAPD) and expressed sequence tag-derivedsimple sequence repeats (EST-SSRs) provided a useful tool for investigating Korean ginseng genetic diversity. In this study,18 polymorphic markers (7 RAPD and 11 EST-SSR) selected to assess the genetic diversity in 31 ginseng accessions (11Korean ginseng cultivars and 20 breeding lines). In RAPD analysis, a total of 53 unique polymorphic bands were obtainedfrom ginseng accessions and number of amplicons ranged from 4 to 11 with a mean of 7.5 bands. Pair-wise genetic similaritycoefficient (Nei) among all pairs of ginseng accessions varied from 0.01 to 0.32, with a mean of 0.11. On the basis of theresulting data, the 31 ginseng accessions were grouped into six clusters. As a result of EST-SSR analysis, 11 EST-SSR mark-ers detected polymorphisms among the 31 ginseng accessions and revealed 49 alleles with a mean of 4.45 alleles per primer.The polymorphism information content (PIC) value ranged from 0.06 to 0.31, with an average of 0.198. The 31 ginsengaccessions were classified into five groups by cluster analysis based on Nei’s genetic distances. Consequently, the results ofginseng-specific RAPD and EST-SSR markers may prove useful for the evaluation of genetic diversity and discrimination ofKorean ginseng cultivars and breeding lines.

This study was conducted to select environmental friendly organic materials for controlling the ginseng alternaria blight and to evaluate their effects from 2011 to 2012. Alternaria blight is caused by Alternaria panax and is the most common ginseng disease in Korea. Environmental friendly organic materials were used to reduce amount of chemical fungicides and the number of spray for control of Ginseng Alternaria leaf blight. In 4 years of ginseng, control value of Alternaria leaf blight by single application of Defenoconazole WP was 82.3% and those of single application was 62.0~75.9%. Consequently, mixed or alternated application of eco-material products could be recommended as a control method to reduce the amount of fungicides.

This study was carried out to investigate the difference of the content of soil chemical components and growth characteristics in five years old ginseng affected by application of manure in paddy-converted field. As all livestock manure regardless of kinds increased along with the whole soil chemical component, including the pH and EC in 2008. Change in the EC of control plot was slightly increased but not exceeded 1 ds/m over the years. However, the changes in the EC of livestock manure regardless of kinds and amounts were highly increased and irregularly exceeded 1.5 ds/m in 2012. The 5 years old ginseng root fresh weight, treatment of fertilizing pig manure compost 4 ton per 10 areas (PMC 4t on/10a) and fowl manure compost 4 ton per 10 areas (FMC 4 ton/10a), were superior to the others. But there were no difference between PMC 4 ton/10a, FMC 4 ton/10a and control. The standing crop rate 39.6%, treatment of fertilizing cattle manure compost 4 ton per 10 areas (CMC 4 ton/10a), was best in all livestock manure. However that was relatively lower than control. Physiological disorder occurrence rates of livestock manure related with leaf and root of ginseng were also higher than that of control. If excessively using non-decomposed livestock manure, It would be caused physiological disorder in many ways. It is a big problem to be producing the quality ginseng. More research is needed to find out the economic and effective fertilizer.

The present study was investigated the effect of planting density on plant growth and yield of Panax ginseng C. A. Meyer. Sowing density is one of the most important factors affecting yield. The value of roots have affected by shape, color, weight and degree of disease injury in ginseng. Also, it needed to minimize elapsed time for 5 years including pre-planting field management and cultivation period. We were conducted to evaluate that planting densities and varieties on the growth, yield and missing rate. The direct sowing was treated seeds density as a four levels (seeds; 72, 90, 120, 144) and 10 different varieties with 3 years old ginseng roots. Root weight was significantly affected by planting density and variety, but the number of lateral root and yield were affected by only planting density. Growth index was related to variety and planting density. Also, Root shape index was affected by both varieties and planting densities. Suitable planting density and variety were 120 plant per 1.62 m2 and Gopoong, respectively. Results showed that it was also a significant difference (p=5%) in variety of planting density on growth.

Korean ginseng (P. ginseng C. A. Meyer) is one of the most important medicinal plant in the world. Understanding genetic variability among the assortment of Korean ginseng is important for breeding. The aim of this study was to molecularly characterize Korean ginseng cultivar and breeding lines through the use of eight previously reported STS markers (MFGp183, MFGp130, MFGp110, UFGp74, UFGp163, MFGp108, MFGp81 and UFGp156). All STS markers produced interpretable electropherograms from 31 accessions consisting of 11 Korean ginseng cultivars and 20 breeding lines. When eight STS markers were combined, we identified to total 19 genetic patterns; in particular, nine cultivars (Chunpoong, Yunpoong, Gopoong, Gumpoong, Sunpoong, Sunone, Cheongseon, Sunhyang, Cheonryang) and 5 breeding lines (G08012, G04079, G04075, G08036, G04110) in ginseng samples can be discriminated from the others. Together with other available markers, these STS markers will contribute to the management of ginseng genetic resources and the protection of breeders' rights.

This study was carried out to investigate change of ginsenoside contents according to tissue ratio in ginseng root by age and diameter. The epidermis-cortex and xylem-pith extent, fresh weight, dry weight of ginseng increased with the root age increase. They increased higher in xylem-pith than in epidermis-cortex. The ratio of epidermis-cortex decreased and xylem-pith increased as the main root diameter increased. In case of same diameter, the xylem-pith ratio increased by the increase of root age. The epidermis-cortex ratio was 4 > 5 > 6 years, respectively. The total 10 ginsenosides of epidermis- cortex increased with the root age increase. However, the total ginsenoside of xylem-pith decreased and it was 2~5 times lower than epidermis-cortex. The most of ginsenoside contents existed in epidermis-cortex. The diameter decrease in main root is related to the increase of epidermis-cortex ratio. It leads to increase of ginsenoside contents. In order to select high level of ginsenoside cultivar, it suggested that it should be selected main root having narrow diameter and lower epidermis- cortex ratio.