Inflammation mainly mediated by innate immune cells as the first line of host defense against pathogens is an acute response that limits tissue damage and eliminates pathogens in the body. In triggering inflammation, several pattern recognition receptors work together; membrane-associated Toll-like receptors, c-type lectin receptors, retinoic acid-inducible gene-like helicase receptors, absent in melanoma-like receptors, and cytosolic nucleotide-binding domain and leucine-rich repeat receptors. Among them, inflammasome is a newly trigger of inflammation in response to exogenous and endogenous stimuli and its activation leads to the assembly of multiprotein platforms composed of NLRP3 (NOD-like receptor family, pyrin domain containing 3), ASC (apoptosis associated speck-like protein containing a CARD), and procaspase 1. Thus, the activated inflammasome activates caspase 1, resulting in processing and secretion of interleukin (IL)-1β. Recent emerging data suggest that dysregulated metabolites, i.e., amyloids, ceramides, and cholesterol crystals, have been classified as inflammasome activators. In addition, IL-1β may play a critical role in the pathogenesis of chronic inflammation-induced disorders such as Alzheimer’s diseases, type 2 diabetes, and atheriosclerosis. This review introduces the basic concept of inflammasome activation and auto-inflammatory diseases. In addition, it discusses the updated signaling models of inflammasome activation that link metabolic dysfunction in order to outline future therapeutic approaches to inflammasome-mediating diseases.

지구 온난화의 영향으로 우리나라는 지난 30년동안 평균기온이 0.7℃, 겨울철에는 1.4℃가 상승하였다. 이러한 온난화로 인하여 우리나라에서는 이상기상 현상이 자주 발생하여 채소작물에 피해가 발생한다. 특히 노지에서 많이 재배되고 있는 고추, 배추 및 무는 온난화로 인하여 정식시기를 점점 앞당겨 정식후 갑작스런 저온이 오면 이들 작물의 피해가 크다. 따라서 본 실험은 저온에 따른 배추의 생육특성과 엽 세포조직에 미치는 영향을 구명하고자 실시하였다. '춘광' 배추품종을 화분에 정식한 후 노지 처리구, 무가온 하우스 및 가온하우스 처리구 등 3처리를 하였다. 그 결과, 정식후 50일의 생육은 노지처리구의 초장, 엽수, 엽록소 및 엽면적이 가온 처리구에 비해서 현저하게 떨어졌고, 특히 생체중의 경우에는 가온 처리구에 비해서 노지와 무가온 하우스 처리구가 1/3 수준으로 현저하게 낮았다. 배추의 잎이 10매 정도 생육이 되었을 때 저온에 따른 배추 잎의 피해증상은 영하 3.0℃ 조건에서는 배추 겉잎에 약간의 수침증상을 보였으나 회복되었다. 그러나 영하 7.4℃ 조건의 배추 잎은 수침증상이 심하였으며 회복되지 못하고 황색으로 변하면서 결국 잎이 고사하였다. 피해받은 잎의 엽육세포는 영하 3.0℃ 조건에서는 울타리조직과 해면조직이 약한 붕괴증상을 보였지만 어느정도 형태를 갖추고 있었는데, 영하 7.4℃ 조건에서는 세포가 동결된 후 해동되는 과정에서 세포의 막구조가 파괴되어 울타리조직과 해면조직이 완전히 붕괴되었기 때문에 세포 형태를 갖추고 있지 않았다. 따라서 배추 정식후 초기 생육 단계에서 영하 3℃까지는 비닐이나 부직포로 보온, 토양수분 조절, ABA 처리를 하여 동해를 예방할 수 있으나 영하 7℃의 저온이 발생하면 세포가 파괴되어 회복하기 어렵기 때문에 다시 심거나 또는 다른 작물로 대체하는 것이 좋을 것으로 사료되었다.

본 논문은 휴대 전화를 이용하여 근거리 무선 통신을 수행할 때 발생하는 과금, 지연, 가입된 이동통신사가 다른 단말 간 통신 등의 제약을 해결하기 위한 방법으로, 블루투스(Bluetooth) 기반의 외부 통신 장치를 제안한다. 제안하는 방법을 통해 블루투스가 내장되어 있지 않은 단말기, 블루투스가 내장되어 있다 하더라도 이를 제어할 수 없는 단말기에서도 근거리 무선 통신을 지원할 수 있게 된다. 또한 WIPI Jlet 기반의 소프트웨어 제작을 통해 제안한 하드웨어를 활용하는 여러 가지 방법에 대해 기술한다. 마지막으로 제작된 외부 통신 장치와 라이브러리를 이용하여 두 개의 게임을 실제로 만들었다. 이러한 게임은 사용자들이 근거리 통신을 통하여 게임을 진행하는 방식이다. 이러한 게임들을 통하여 일반 기능폰 간의 통신뿐만 아니라 기능 폰과 Windows Mobile과 같은 스마트폰과의 통신을 통한 다양한 활용성에 대해 기술한다.

The aim of this study is to find out histomorphologic change and cellular activity of condyle resulted from unilateral mastication by comparison of cell proliferation and apoptosis activity. 30 adult rats were dived to 15 experimental group and 15 control group randomly. Right upper and l ower molars were gently extracted in experimental group, to make unilateral mastication environment. All subjects were sacrificed at 1 week, 2 weeks and 4 weeks by chloroform, and their tissues were prepare to observation. Streptovidin-biotin system for BrdU stanning, was used to determine cellular proliferative activity. TUNEL method was used to determine apoptotic activity. The result for cellular activity was recorded at both of anterior portion and posterior portion of condyle. Hematoxylin and Eosin stanning was used for histiomorphological change. The results were as follows. There were more change in superficial layer than deep layer of condyle in cellular activity. In anterior portion of condyle cartilage, cellular proliferative activity of experimental group was lower than control group and apoptotic activity of experimental group was higher than control group. And apoptotic activity of extracted side in experimental group is the most. In posterior portion of condyle cartilage, cellular proliferative activity of extracted side in experimental group was higher than non-extracted side and control group, And apoptotic activity of extracted side in experimental group was the low. As a result of histomorphological change, there was hyperplasia in posterior region o f extracted side c ondyle i n experimental g roup, but t here was n o change i n unextracted side i n experimental group. There was histomorphological hyperplasia in posterior condyle of experimental group as results of high cellular proliferative activity. There was mainly apoptotic change of anterior portion condyle in experimental group. But there was no histomorphologic change. In other words, there was hyperplasia by increasing of cellular proliferative activity in posterior portion of nonfunctional side condyle. In functional side condyle, there was no histomorphological change in functional condyle, but there was change in cellular activity.

보행교통류를 주제로 다양한 연구들이 진행되었지만 초기의 보행연구는 차량의 교통류이론을 그대로 적용하여 해석하기도 하였다. 최근 보행교통류에 대한 다양한 모형들이 제시되고 있으며, 특히 CA모형은 보행교통류를 위한 시뮬레이션 모형으로 빈번하게 사용되고 있다. 대표적인 CA 모형으로 가스입자의 움직임을 이용하여 양방향의 보행교통류를 설명하기도 하였는데 초기에는 정방형의 Gas-lattice 모형이 제안되었으며 이후에 정육방 Gas-lattice 모형을 이용하여 보행자의 움직임과 회피를 묘사하기도 하였다. 하지만 이러한 모형들은 보행자의 움직임을 편의임의보행으로 가정하였기 때문에 단일방향으로의 움직임만을 설명할 수 있었다. 본 연구에서 제시된 MLPM(the Multi-Layer Pedestrian Model)은 어떤 공간에서 복수개의 기종점을 가진 경우에도 현실적인 보행자의 움직임을 설명할 수 있는 모형이다.

This study was performed to investigate the effects of Needle Electrode Electrical Stimulation(NEES) on ischemia-induced cere˗ brovascular accidents. After obstruction and reperfusion of arteries in white mice, the amounts of necrosis and inflammation related sub˗ stances Bax, IL-6, Caspase-3, and COX-2 were measured in neurons of the fore-brain. The following results were obtained. This study used 21 male specific pathogen free(SPF) SD rats, 8 weeks of age and approximately 300g in weight. Each exposed artery was completely occluded with non-absorbent suture thread and kept in that state for 5 minutes. The sutures were then removed to allow reperfusion of blood. Test group is control group(common carotid artery occlusion models), a GI(underwent common carotid artery occlusion), and NEES(underwent NEES after artery occlusion). The GI and NEES groups were given 12, 24, or 48 hours of reperfusion before NEES. NEES device(PG6, ITO, Japan, 9V, current, 2Hz) was used to stimulate the bilateral acupoint ST36 of the SD rats for 30 minutes while they were sedated with 3% isoflurane. An immuno-his˗ tochemistry test was done on the forebrains of the GI induced rats. Both Bax and Caspase-3 immuno-reactive cells, related to apoptosis, were greater in the GI than the NEES group. Cox-2 and IL-6 immuno-reactive cells, related to inflammation, were greater in the GI and NEES groups than the control group. We can expect that applying NEES after ischemic CVA is effective for preventing brain cells from being destroyed. And we can conclude NEES should be applyed on early stage of ischemic CVA.

본 연구는 배추 재배시 기후변화에 대비하여 고온과 고농도의 이산화탄소 조건에서 생육반응과 세포조직의 변화를 알아보고자 수행하였다. 처리는 '대기중 온도+대기중 CO2 농도(Control)', '대기중 온도+대기보다 CO2 2배 상승(Elevated CO2)' '(대기온도보다 4℃ 상승+대기중 CO2 농도(Elevated temp.)', '대기온도보다 4℃ 상승+대기보다 CO2 2배 상승(Elevated temp.+CO2)'의 4가지 조건으로 처리하였다. 그 결과, 대기조건보다 온도만 높아지게 되면 배추의 생체중이 현저하게 저하되어 수량이 떨어지는 것으로 나타났고, 온도가 높아지고 CO2 농도가 동시에 올라가게 되면 생육이 어느정도 회복되는 것으로 나타났다. 또한 칼륨과 인산함량은 처리별로 차이가 없었지만 칼슘과 총질소함량은 온도만 높인 처리에서 높았고, 마그네슘 함량은 온도와 CO2 농도를 동시에 높인 처리에서 높게 나타났다. 배추잎의 세포 관찰결과 CO2 농도를 2배 정도 높인 처리에서 잎내의 전분함량이 증가하는 것으로 나타났고, 온도만 높인 처리에서 전분 함량이 가장 낮았다.

온라인 게임에 대한 수요가 증가하면서 많은 사용자들은 다양한 무선 접속 기술들을 이용하여 장소에 구애받지 않고 게임 컨텐츠를 이용할 수 있게 되었다. 모바일 기반에서 온라인 게임 컨텐츠 및 시나리오를 신속하게 내려받기 위해서는 무선링크를 통한 전송속도가 충분히 확보되어야 한다. 본 논문에서는 이동/무선 환경에서 고속의 데이터 전송을 지원하는 대표적인 3세대 이동통신 표준인 WCDMA/HSDPA 시스템의 기본적인 개념과 핵심 요소들을 소개하고 온라인 게임의 향후 발전 방향을 전망한다.

In our present study, total methanol extracts prepared from B. platyphylla var. japonica showed a significant increase in cell proliferation upon the induction of oxidative stress by hydrogen peroxide or y-ray irradiation. Total methanol extracts were fractionated into five separate preparations i.e. n-hexane, dichloromethane, ethylacetate, n-butanol and water fractions. Among these, the ethylacetate and butanol fractions of B. platyphylla var. japonica showed the highest protective effects against oxidative stress induced by hydrogen peroxide. These fractions also showed strong protective effects against y-ray irradiation. When we evaluated the cytotoxicity of these fractions, the butanol fraction showed no effects in a colony formation assay. In addition, the butanol fraction showed a cell proliferation activation effect evidenced by significant increase in the colony formation of y-ray irradiated cells. Both a radical scavenging activity and clonogenic activity assay suggested that the mechanism behind this protective effect against reactive oxygen species may be due to the radical scavenging and cell proliferation activity of B. platyphylla var. japonica extracts.

A cell is the product of a long period of evolution and can be represented as an optimized system (homeostasis). Stimuli from the outside environment are received by sensory apparatus on the surface of the cell and transferred through complicated pathways

본 연구의 선행연구에서 사용자가 제품 사용 중 표출된 감성을 보다 간단하고 손쉽게 평가할 수 있도록 자기-보고 질문지형 스케일을 개발하였고, 본 연구에서는 휴대폰 사용 중 표출되는 사용자의 감성을 평가해 보았다. 전반적으로 사용성 측면을 상당히 고려하여 디자인한 제품이라고 할 수 있는 휴대폰의 경우 높은 '사용적 만족성'과 낮은 '불편성'이 표출되었다. 반면에 전반적으로 새로운 기능과 스타일에만 집중하여 사용자의 사용성 측면에 대해서는 다소 불편한 제품이라고 할 수 있는 휴대폰의 경우 낮은 '사용적 만족성', 높은 '불편성'이 표출되었지만 그 외 '심미성', '신규성', '유쾌성', '우수성'에서는 높은 값을 나타내었다. 본 연구의 결과를 가지고 성급하게 제품의 사용성과 사용자의 감성에 대한 결론을 내리기에는 다소 미흡하지만 향후 제품 경험 시간에 따라 표출되는 사용자의 감성변화를 분석함으로써 제품의 사용성과 사용자의 감성간의 관계를 보다 더 명확하게 밝혀낼 수 있으리라 기대한다.

Phospholipase A2 (PLA2) is the committed catalytic step of eicosanoid biosynthesis, which has been a common molecular target of several entomopathogens to induce insect immunosuppression. Despite critical importance of PLA2 in insect immunity, its gene structure was not known. This study identified insect PLA2 gene associated with immune reactions in the red flour beetle, Tribolium castaneum. Based on a previous study that an immune-associated PLA2 in insect is secretory type of PLA2 (sPLA2), five highly matched cDNA sequences were obtained from T. castaneum genome database using an sPLA2 sequence probe encoded in Drosophila melanogaster. The expressions of these five putative PLA2 were confirmed by reverse transcriptase-polymerase chain reaction. Out of five genes, one PLA2 gene called TcPLA2B was chosen because it showed specific expression in hemocyte and fat body. TcPLA2B was cloned and expressed in Escherichia coli and its protein was purified. The purified TcPLA2B showed PLA2enzyme activity, which was specifically inhibited by bromophenacyl bromide (a specific sPLA2inhibitor) and dithiothreitol (reducing agent of disulfide bond). It was sensitive to pH (optimum at pH 6.0) and reaction temperature (optimum at 10-30°C), and calcium dependency. An immunofluorescence assay indicated that TcPLA2B was localized near to cellular membrane of the cytosol in the hemocytes of T. castaneum at immune chanlenge. Double-stranded RNA (dsRNA) of TcPLA2B-treated larvae showed knockdown of its mRNA expression and did not form hemocyte nodule formation, while control larvae could exhibit time- and bacterial dose-dependent nodule formation in response to bacterial challenge. Addition of arachidonic acid (the catalytic product of PLA2) to the dsRNA-treated larvae rescued the inhibition of nodule formation. These results suggest that TcPLA2B gene is associated with insect immune reaction.

Hemolin is a member of the immunoglobulin (Ig) superfamily and contains four Ig domainsthat are similar to neural cell adhesion molecules. It has been regarded as a recognition molecule at immune challenge in insects. This study showed that hemolin of Plutella xylostella was expressed during pupal and adult stages but absent in all larval instars without any immune challenge. It is, however, strongly induced by the injection of Escherichia coli or its lipopolysaccharide in hemocytes, fat body and gut. A double-stranded RNA (dsRNA) interference experiment revealedits role in activation of prophenoloxidase (PPO) in the hemolymph during bacterial infection. Also its involvement in cellular defense was investigated in its mediation of the adherence of hemocytes to rat blood erythrocytes which was knocked down by its dsRNA. Finally, its physiological significance in pupal stage was confirmed by using dsRNA, which significantly prevented adult development. Therefore, it is concluded that hemolin plays roles in both immune and adult development in P. xylostella.

As the use of vehicle route application and LBS(location based service) are fast grew, the importance of maintaining road network data is also increased. To maintain road data accuracy, we can collect road data by driving real roads with probe vehicle, or using digital image processing for the extraction of roads from aerial imagery. After compare the new road data to current database, we can update the road database, but that job is mostly time and money consuming or can be inaccurate. In this paper, an updating method of using GPS(global positioning system) enabled cell phone is proposed. By using GPS phone, we can update road database easily and sufficiently accurately.