Buckwheat sprout is used as vegetable, and also flour for making noodles, and so on. Currently, information about tissue culture in buckwheat is limited and restricted to micropropagation. We carried out somatic embryogenesis and plant regeneration using hypocotyl segments as explant of the cultivated buckwheat species Fagopyrum esculentum, differs from existing studies in the growth regulator combinations used. Maximum callus regeneration was induced on MS medium containing 2,4-D(2.0 mg/L) and benzylaminopurine BAP (1.0 mg/L) and 3% sucrose. Friable callus was transferred to solidified MS media containing BAP (1.0 mg/L) and at various concentrations for the induction of embryogensis. The optimum concentrations of additives were IAA (2 mg/L), KIN(1.0 mg/L), BAP (1.0 mg/L), and 3% (w/v) sucrose. Only 2,4-D did not show any significant effect on callus induction or embryogenesis. Regeneration of embryonic callus varied from 5 % to 20%. Whole plants were obtained at high frequencies when the embryogenic calluses with somatic embryos and organized shoot primordia were transferred to MS media with 3% sucrose. Regenerated plants after acclimation will transfer to green house. The main objective of this research was to develop a efficient protocol for plant regeneration for common buckwheat, and to apply in future for genetic transformation.

총경동맥은 심장으로부터 혈액을 직접 대뇌로 전달하는 동맥 혈류 순환이 중요하며, 특히 동맥 혈관내의 혈액의 흐름을 방해하는 여러 변인들이 있다. 그 변인 중에서도 평균혈류속도, 맥박지수, 및 혈류 저항 변인 등이 있는데,이중 맥박지수나 혈류저항지수는 최대 수축기와 이완기시 혈류속도와 관련이 되어있기 때문에 혈압과도 중요한 변인들이 된다, 이와 같은 뇌혈류 속도 변인들의 변화에 대해서 관찰하는 데는 비침습적인 초음파를 이용한 도플러 뇌혈류 측정법을 이 용한다. 최대운동은 20대 남성에서 뇌로가는 혈류인 총경동맥의 혈류속도를 증가 시킬 수있는 것으로 나타났다.

Single seeds of common buckwheat cultivar Suwon No. 1 when subjected to SDS-PAGE revealed very high polymorphism. High variation existed for protein or protein subunits with molecular weight 54-47kDa, 45-25kDa and 16-11kDa. The electrophoregram showed variation for globulin as well as other protein fractions. About 300 proteins were separated by two-dimensional electrophoresis in common buckwheat (Fagopyrum esculentum Moench.) seed. Seed maturation is a dynamic and temporally regulated phase of seed development that determines the composition of storage proteins reserves in mature seeds. Buckwheat seeds from 5, 10, 15, 20, and 25 days after pollination and matured stage were used for the analysis. This led to the establishment of high-resolution proteome reference maps, expression profiles of 48 spots. It was identified 48 proteins from MALDI-TOF/MS analysis of wild buckwheat seed storage proteins. The 48 proteins were found identical or similar to those of proteins reported in buckwheat and other plants; it is belonging to 9 major functional categories including seed storage proteins, stress/defense response, protein synthesis, photosynthesis, allergy proteins, amino acid, enzyme, metabolism, and miscellaneous. It appears that the major allergenic storage protein separated played the important role in buckwheat breeding and biochemical characterization.

DirectX를 사용하는 윈도우 게임 및 콘솔 게임은 전 세계 게임 시장의 20% 이상을 차지하고 있으며, 전 세계 시장의 75% 이상이 C++로 개발되고 있다. 본 연구는 DirectX와 C++ 기반으로 개발된 20개 프로젝트 코드의 공통성과 가변성을 분석하여 패턴화하고, 이를 공통 모듈화 하였으며, 이 모듈을 모형화 하였다. 사례 연구를 통해, 제안한 기법을 사용하여 DirectX와 C++ 기반으로 개발된 소프트웨어 코드의 생산성이 60% 이상 개선됨을 입증하였다.

수년 동안 국내 전역에서 수집된 기장 137 유전자원을 공시하여 농업적 특성조사를 통한 품종육성의 기초자료를 제공코자 수행하였던 바 그 결과를 요약하면 다음과 같다. 1. 공시된 137자원의 수형은 편수형, 산수형, 편밀수형(편수형과 밀수형의 중간형태) 및 산편수형(산수형 과 편수형의 중간 형태)의 4가지 수형으로 구분되었다. 수형별 빈도를 보면 편수형이 91자원으로 66.4%를 차지하였다. 2. 종피색은 황백색, 황갈색, 흑갈색, 황색 및 갈색 5가지로 분류되었으며, 종피색별 빈도를 보면 황백색 이 54자원 39.4%로 가장 많았으며, 종실형태는 방추형이 62.8%, 단방 추형이 37.2%이었다. 3. 출수소요일은 68~85 일의 범위를 나타내어 변이폭이 컷으며, 평균은 73.7일이었다. 빈도별 분포는 70~75 일이 46.7%로 가장 많았다. 생육기간은 109~124 일의 범위를 나타내었고, 평균 112일이었다. 생 육기간의 빈도를 보면 111~115 일이 83자원으로 60.6%로 가장 많았다. 4. 간장의 분포는 181~190cm 가 65자원으로 47.4%로 가장 많았으며, 160 cm 이하가 2.9%로 가장 적었다. 수장은 156~201cm 의 범위를 보였고, 평균 181.4 cm이었으며, 빈도별 분포는 31~35cm 가 74자원으로 54.0%로 가장 많았다. 5. 1수립수의 범위는 432~2,665 개로 변이폭이 상당히 컷으며, 평균은 1,429.5개이었다. 1수립수 분포는 1,200~1,400 개가 20.4%로 가장 많았으며, 1,000개 이하 및 2,000개 이상이 각각 13.1%, 8.6%를 차지 하였다. 6. 등숙률은 10% 이하에서 90% 이상까지 변이의 폭이 매우 컷다. 1000립중은 범위가 2.2~6.0g 으로 변 이폭이 상당히 컷으며, 평균은 4.69 g이었다. 7. 간장은 수장 및 출수소요일과 유의한 정의 상관관계를 나타내었으며, 생육기간은 1000립중과 유의한 정의 상관관계를 나타내었다. 등숙률도 1000립중과 유의한 정의 상관관계를 보여 등숙률이 높은 자 원일수록 1000립중이 높았다.

Buckwheat is one of the traditional crops and has become a renewed target of interest or a popular crop as a healthy foodstuff, because it is a good source of cereal protein which is rich with essential amino acids. However, what is critical to our health is that buckwheat contains proteins which cause a allergy. Buckwheat allergy resulting from ingestion is caused by the storage proteins in the grain with molecular weights ranging from 15, 22, 35, 39 and 50 kDa proteins of the inner fractions to low, and there were clear differences in the protein compositions between the inner and outer buckwheat flour fractions. A major allergenic protein of buckwheat is Fag e 1 with molecular weight 22 kDa (BW22KD). Buckwheat allergy is an immunoglobulin E (IgE)-mediated hypersensitive response capable of causing anaphylactic shock. Buckwheat seeds were dissected to endosperm and embryo. From each fraction we extracted proteins and analyzed extracts by SDS-PAGE and 2-DE. On electrophoregrams of endosperm proteins, 6 intense bands were detected. The most intense corresponded to molecular weights ranging from 54 to 65 kDa. These proteins have been reported not to be allergenic. We show here that the allergenic buckwheat seed proteins are found only among embryo proteins. No allergenic proteins were found in the buckwheat endosperm. The results presented here lead to the proposal that patients with hypersensitivity to buckwheat flour should use only fine flour from buckwheat endosperm, as this fraction contains no allergenic proteins. At present, specific protein spots will be selected and in-gel digested for MALDI-TOF-TOF/MS analysis.

Crop characteristics of 137 common millet(Panicum miliaceum L.) resources collected in Korea were investigated in order to establish basic data for the improvement of crop breeding. Spike types of 137 plant resources were classified as 4 types of oblate, spreading, oblate-compact(intermediary type between oblate and compact) and oblate-spreading(intermediary type between oblate and compact) type, of which oblate spike type was the highest percentage of 66.4%(91 plant resources) of 137 germplasm. Seed color was classified as 5 colors of dark brown, yellow, brown, yellowish brown, and yellowish white, of which yellowish white was the highest percentage of 66.4%(54 plant resources) among them. Seed type was classified as 2 types of pyramid shape and short pyramid shape, which showed 62.8% and 37.2% respectively. Days from seeding to heading date showed the range from 68 to 85 days with 73.7 days in the average and the highest frequency proportion of it was the group from 70 to 75 days, which occupied 46.7%(64 plant resources) of 137 germplasm. Days from seeding to physiological maturity showed the range from 109 to 124 days with 112.0 days in the average and the highest frequency proportion(60.6%) of it was the group from 111 to 115 days, while there were also occupied with 6.6% below 110 days and 5.1% over 120 days among 137 germplasm. Group from 181cm to 190cm of culm length showed the highest frequency proportion of 47.4%, whereas below 160cm was the lowest occupancy of 2.9%. Spike length showed the range from 181 to 190cm with 181.4cm in the average and the highest frequency distribution of it was the group from 31 to 35cm with the proportion of 54.0%. Number of grains per spike showed the range from 432 to 2,665 grains with 1,429.5 grains in the average and group from 1,200 to 1,400 grains per spike was the highest frequency distribution with the proportion of 20.4%. There were also occupied with 13.1% below 1,000 grains and 8.6% over 2,000 grains per spike among 137 germplasm. Culm length and spike length were positively significant correlation coefficient with days from seeding to heading date. Days from seeding to physiological maturity and percentage of ripened grains were highly significant positive correlation coefficient with 1000 grain weight, showing that the longer and higher the days from seeding to physiological maturity and percentage of ripened grains, the higher 1000 grain weight.

본 연구의 목적은 올림픽 국가대표 선수들과 일반선수들의 자기관리 프로파일을 비교하여 분석해 보는데 있다. 올림픽 국가대표 선수들은 2004년 아테네 올림픽 대표선수로 발탁되어 태능 선수촌에서 훈련 중인 261명(남:134, 여:127)의 선수들이 총17개 총목에서 군집추출방법에 의해 표집되었다. 그리고 일반 선수들은 고등학교, 대학 및 실업팀에서 국가대표 경력이 전혀 없는 252명(남:192, 여:60)의 선수들이 총 15개 종목에서 참가하였다. 이번 연구에서는 선수들이 경기에 임하는 자세 및 태도를 묻는 질문 3문항과 허정훈(2003)이 개발한 운동선수 자기관리 검사지(ASMQ)를 활용하였고 경기력과 성별에 따라 기술통계와 t-test를 실시하였다. 올림픽 대표선수들의 63% 정도가 메달을 획득하는 것에 최고의 출전가치를 두고 있었으며, 일반선수들의 경우 자신의 기록경신과 만족할 만한 플레이를 펼치는 것을 상대적으로 더 중요시 하였다. 그리고 올림픽 대표선수와 일반선수 모두 최고의 경기력을 펼치는데 가장 중요한 요인으로 자신감과 집중력을 꼽아 심리․정신적 자기 관리의 중요성을 깊게 인식하고 있었다. 남자 국가 대표 선수들의 경우 자기관리 하위영역인 몸관리(p<.01), 대인관리(p<.05), 훈련관리(p<.05), 정신력 관리(p<.001) 요인에서 일반 남자선수 집단에 비해 유의하게 높은 수준을 보여 주었으나, 여자 선수들의 경우에는 훈련관리(p<.05) 영역에서만 유의한 차이를 나타냈고 다른 요인에서는 차이가 없었다. 또한 남자 국가대표 선수들의 경우 정신력 관리 수준이 다른 집단에 비해 특출하게 높은 형태를 보여주었다. 올림픽 국가대표 선수든 일반선수든 몸관리 수준은 전반적으로 낮은 반면, 대인관리 수준이 상대적으로 높은 형태로 동일한 경향성의 자기관리 프로파일이 발견되었다.

비파괴 검사 기술은 현존하는 구조물의 진단을 위해 빈번하게 사용되었고, 사용되고 있다. 이러한 검사들로 본 연구에서 사용된 검사로는 육안적 검사, 해머 사운딩, 슈미트 해머, 그리고 토모그래피를 포함한 초음파 검사 등이 있다. 완료된 비파괴 검사 결과는 국부적으로 수행될 파괴검사의 위치를 산정하는데 사용되었다. 그래서, 압축강도, 염화물, 암석 분류 실험으로 구성된 파괴검사를 수행하였다. 비파괴검사와 파괴검사의 결과들을 종합하여 교각두부의 건전성을 평가하였다. 본 논문에서는 파괴 검사와 비파괴 검사의 적용과 해석, 그리고 이어지는 보수, 보강, 유지 결정 과정에 대해서 나타내었다.

Lately, the autumn cropping areas of potato have been steadily increased to supply fresh potatoes all year round in Korea. Although "Dejima" is a main cultivar for autumn cropping, it is very susceptible to common scab (Streptomyces scabies) and show high frequency of physiological disorders such as knobs and cracking on tubers. "Chudong" was bred in 2002 for fresh table use from a cross between H83520-3 with short tuber dormancy and "Superior" with common scab resistance. It has attractive, round tuber shape with white skin and flesh colors. Its tuber dormant periods is 5 days longer than 65 days of "Dejima". Its average yields were 32.4 and 21.8 ton/ha at 90 days after planting in spring and autumn cropping of regional yield trials, respectively. It is more resistant to common scab and potato leaf roll virus and less susceptible to late blight (Phythophthora infestans) compared to "Dejima". However, it is susceptible to potato virus Y.

‘Seohong’ was bred within the potato breeding program of National Institute of Highland Agriculture (NIHA). It was originated from a cross made in 1997 between ‘Jashim’ as a female and 93K65-1 as a male parent. Seohong was evaluated as a seedling number 97S05-1 for tuber characteristics, growth and yielding ability from 2001 to 2002, and than this clone was renamed as Daegwan 1-82. This clone was evaluated in the regional yield trial and the disease resistance in the five locations for three years. Finally, this clone was named as ‘Seohong’ and registered a new recommended potato cultivar in 2006. This cultivar has a round, shallow eye depth, light red-skinned tuber characteristics and low to medium dry matter content. It showed high resistance to common scab (Streptomyces scabies) and potato leaf roll virus (PLRV), however it was susceptible to potato virus Y (PVY) and late blight (Phytophthora infestans). Its mean tuber yields from the regional yield trial and winter cropping trial were 12 and 49 percent higher than that of ‘Superior’, respectively.

A network-based robot [1] is a robot that explores service servers in the network environment for analyzing sensor data and making decision. Since network-based robot architecture was proposed, it’s possible to reduce costs of robots. We hope robots would be all around at home environment. Therefore, normal users who are not experts need to be able to control those robots by using easy commands. We developed a scripting language, named CCSLR, to help users and developers who control various robots in ubiquitous environment. We focused on how to design the common language for various robots and how to translate a CCSLR script into a sequence of low-level commands of the target robot. In this paper, we propose scripting methods, with three layers. The CCSLR system reads the profile information from the knowledge representation database. Users don’t have to know all about kinematical and mechanical details of a robot. Then again, the CCSLR system will use the profile information to translate the script into separated executable library commands. The CCSLR system manages robot’s changing state every time a robot executes a command.

Antioxidant activity of the extract fractions from leaves, stems, roots and flowers of Cirsium pendulum Fisch. was investigated. The results showed the greatest antioxidant activities in leaves by Rancimat, TBA and DPPH methods. Extracts of common thistle plants dose-dependently increased DPPH free radical scavenging activity, The extract from flowers and its hexane fraction showed the strongest antioxidant activity. HPLC analysis showed that BuOH fraction of the leaves had the highest amount of antioxidant chlorogenic and p-coumaric acids at 5.38 and 9.71 mg 100 g-1, respectively. It implies that common thistle plants had potent antioxidant activity, and their activities were differently exhibited depending on plant part and solvent fraction.

Common thistle contains water-soluble substances that are phytotoxic to neighboring plant species. A series of aqueous extracts from leaves, stems, roots and flowers of common thistle (Cirsium pendulum Fisch.) were assayed against alfalfa (Medicago sativa) seedlings to determine their allelopathy, and the results showed highest inhibition in the extracts from flowers and leaves, and followed by stems, and roots. The extracts at 40 g dry tissue L-1(g~;L-1) applied on filter paper in a Petri-dish significantly inhibited root growth of test plant by 87%. Methanol extracts at 100 g L-1 from leaves inhibited root growth of alfalfa and barnyardgrass (Echinochloa crus-galli) by 89 and 98%, respectively. Hexane and ethylacetate fractions of common thistle reduced alfalfa root growth more than did butanol and water fractions. Incorporation into soil with the leaf residues at 100g~;kg-1 inhibited shoot fresh weights of barnyardgrass and eclipta (Eclipta prostrate) by 88 and 58%, respectively, showing higher sensitivity in grass species. These results suggest that common thistle plants had allelopathic potential for eco-friendly vegetation management, and that especially their activities were differently exhibited depending on plant part.

본 연구는 단메밀과 타타리메밀의 페놀성화합물의 함량을 측정하고 분석, 평가한으로써 생리활성 연구의 기초 자료와 건강기능성 식품소재 개발에도 이용될 수 있는 유용한 기초 자료를 얻고자 수행하였다. 1. 총 페놀의 함량은 타타리메밀 종자가 단메밀 종자보다 약 2배정도 높게 나타났다. 총 페놀 중의 플라보노이드 함량은 단메밀이 약 50~% , 타타리메밀이 약 95~% 를 차지하고 있다. 단메밀과 타타리메밀의 총 페놀 및 플라보노이드 함량은 메밀쌀이 메밀껍질보다 높았으나, 단메밀의 플라보노이드 함량은 메밀껍질 더 높았다. 3. 단메밀의 rutin함량은 메밀껍질(25.2 mg/100g)>껍질을 벗기지 않은 종자(19.8 mg/100 g)>메밀쌀(12.8 mg/100 g)의 순으로 높았으나, 타타리메밀은 메밀쌀(2042.1mg/100g)>껍질을 벗기지 않은 종자(1375.8 mg/100g)>메밀껍질(138.7 mg/100 g)의 순으로 높게 나타났다. 식물체 부위별 rutin의 함량은 단메밀이 메밀싹>잎>줄기>종자의 순이고, 타타리메밀은 잎enli메밀싹>종자>줄기의 순으로 나타났다. 모든 부위에서 타타리메밀이 단메밀보다 높은 rutin함량을 나타냈다. 4. 단메밀과 타타리메밀 종자의 flavanols함량은 큰 차이를 보이지 않았으나, 잎, 줄기, 싹나물부위의 함량은 타타리메밀이 높은 경향을 보였고, 두 종의 flavonols의 함량도 단메밀보다 타타리메밀에서 월등히 높은 경향을 보였다. 5. 식물체 부위별 flavanols의 함량은 단메밀과 타타리메밀의 메밀싹>잎>줄기>종자의 순으로 높게 나타났다. 모든 부위에서 catechin> Epicatechin> Epicatechingallate의 순으로 높게 나타났으나, 메밀싹은 두 종 모두 Epicatechingallate의 함량이 종자의 약 30~~40 배, 잎, 줄기보다 약 15~~20 배가량 높았다.