In 1951, Colin Russell Austin and Min Chueh Chang identified “capacitation”, a special process involving ejaculated spermatozoa in the female reproductive tract. Capacitation is a phenomenon that occurs in vivo , but almost all knowledge of capacitation has been obtained from in vitro studies. Therefore, numerous trials have been performed to establish in vitro capacitation methods for various studies on reproduction. Although a series of studies have been conducted to develop an optimal protocol for inducing capacitation, most have focused on identifying the appropriate chemical compounds to induce the capacitation of boar spermatozoa in vitro. Therefore, the purpose of this study was to identify the optimal incubation time for inducing capacitation in vitro. Duroc semen was incubated for various periods (60, 90, and 120 min) to induce capacitation. Sperm function (sperm motility, motion kinematic parameters, and capacitation status) was evaluated. The results showed that total sperm motility, rapid sperm motility, progressive sperm motility, curvilinear velocity, and average path velocity significantly decreased in a time-dependent manner. However, the capacitation status did not show any significant changes. Taken together, these results indicate that an incubation time of more than 60 min suppresses sperm motility and motion kinematic parameters. Therefore, we suggest that 60 min may be the best incubation time to induce capacitation without negative effects on sperm motility and motion kinematics in boar spermatozoa in vitro.

Reactive oxygen species (ROS) production and F-actin cytoskeleton dynamics play important roles in the survival rate of blastocysts after the vitrifiedwarming process. However, the protective effects of Mito-TEMPO against cryo-injury and viability through F-actin aggregation and mitochondrial-specific ROS production in vitrificated-warmed bovine embryos have not been investigated. The aims of the present study were to: (1) determine the effects of Mito-TEMPO on embryonic developmental competence and quality by F-actin stabilization during in vitro culturing (IVC), and (2) confirm the effects of Mito-TEMPO through F-actin structure on the cryotolerance of vitrification-warming in Mito-TEMPO exposed in vitro production (IVP) of bovine blastocysts. Bovine zygotes were cultured with 0.1 μM Mito-TEMPO treatment for 2 days of IVC. Mito-TEMPO (0.1 μM) exposed bovine embryos slightly improved in blastocyst developmental rates compared to the non-treated group. Moreover, the viability of vitrified-warmed blastocysts from Mito-TEMPO treated embryos significantly increased (p < 0.05, non-treated group: 66.7 ± 3.2% vs Mito-TEMPO treated group: 79.2 ± 5.9%; re-expanded at 24 hours). Mito-TEMPO exposed embryos strengthened the F-actin structure and arrangement in the blastocyst after vitrification-warming. Furthermore, the addition of Mito-TEMPO into the IVC medium enhanced embryonic survival and quality through F-actin stabilization after the vitrification-warming procedure. Overall, our results suggest that supplementing the culture with 0.1 μM Mito- TEMPO improves the embryonic quality and cryo-survival of IVP bovine blastocysts.

Wild birds, especially aquatic birds, are the natural reservoir of avian influenza virus (AIV), and many kinds of water body can be contaminated with feces of these birds. Seasonally, AIVs can be dissolved in the environmental water from the feces of the infected birds, and this water can be a target for viral detection and identification. In this study, we employed and tested three different filters for concentrating AIV, and it was shown that high concentration factor in terms of viral density could be achieved with viral samples diluted with natural water. Wild bird fecal samples containing low pathogenicity H5 AIVs were successfully concentrated with the adsorption and elution method using mixed cellulose esters membrane; the recovery rate of virus was 35.5 % and the concentration factor was about 50 on average. For the larger volume of water sample, we proved that an inline disposable filter with high surface area, 300 cm2, has a comparable concentration factor to the adsorption and elution method and the filter could be used in the field conveniently by being plugged into peristaltic pump. These validated methods for water sampling may be used as a supplementary for virological surveillance on wild migratory birds or during the epidemiological investigation on the environment near affected premises by AIV.

논문의 목적은 예이츠의 초기 글에서 예이츠의 문학에 대한 생각이 어떻게 발전되어 가고 있는지 살펴보는데 있다. 그 중에서도 글에서는 특히 사무엘 퍼거슨 및 클래런스 맨건과 같은 아일랜드 작가, 그리고 보다 전반적인 아일랜드 문학에 대한 바람과 우려에 대해 예이츠가 어떻게 논의하고 있는지에 대해 알아볼 것이다. 그러한 점에서, 이 논문에서는 선배 작가에 대한 예이츠의 글, 그리고 그가 아일랜드의 문학적 어젠다를 수립해 가는 과정에서 겪었던 문제에 대해 이야기 하는 과정에서 훗날 예이츠 자신의 시와 드라마가 나아갈 방향에 대한 단초를 제공하는지에 대해 논의하고자 한다.

Maritime navigation is an important mode of transportation in the country. With the development of the country and the world, there will inevitably be higher requirements for the safe transportation of ships in the future. Therefore, intelligent ships have become the latest research direction in ship design. And with the gradual development and improvement of big data and artificial intelligence technology in the industrial field, smart ships are an inevitable trend in the development of the shipbuilding industry. Smart ships need to maintain the stability of the ship to ensure the safety of the ship when operating at sea. Therefore, the research on the autonomous stowage of ballast water for smart ships in the future is very important. This paper analyzes the development background of intelligent ships, introduces the ship optimization model and optimization design algorithm, and speculates the future development direction of intelligent ships.

Purpose: To develop a tool for measuring peripheral intravenous catheter insertion performance. Methods: This was a cross-sectional descriptive study. Initial items were generated using existing guidelines and tools, and 19 experts assessed content validity. Data were collected from a tertiary hospital in D city from January 17, 2018, to October 20, 2019. The data of 365 nurses were used for principal component analysis with orthogonal rotation. Concurrent validity was confirmed using Pearson’s correlation between the developed tool and the nursing career period. Reliability was confirmed after evaluating internal consistency. Results: Initially, 16 items were generated, but three items were deleted in content validation. A two-factor solution was preferred in exploratory factor analysis and explained 48.2% of the variance. The two factors were named “point of care” and “follow-up care,” respectively. The tool’s reliability is .87. Conclusion: The developed tool was valid and reliable. It is composed of 13 items and can be used quickly and easily. Therefore, it can be utilized for the education and training of novice nurses and the self-reflection of experienced nurses. In addition, it will be useful to review the catheterization process in common clinical settings.

This study is to develop a radish skin peeling machine. Currently, radish peeling work is carried out manually, so the unit price is high due to the increase in labor cost, and problems related to hygiene continue to occur. To solve this problem, a field supply type radish skin peeling machine was designed and manufactured. The peeling machine is a principle in which the skin is removed by conveying the radish using a roller and passing through a blade assembly. In this paper, the design and analysis of the roller and blade part applied during the movement of the radish were carried out, and structurally stable results were obtained.

We propose a method for developing an in-pipe inspection robot based on multiple inertial sensors. Estimating the position of underground pipelines where satellite signals do not reach remains challenging. High-precision inertial sensors and high-tech mobile robots can be solutions, but their high price limits their general use. We developed an in-pipe inspection robot by combining various low-cost sensors with a microcomputer-based RC car platform. First, we fabricated a multi-inertial sensors module by combining commercial grade low-cost MEMS inertial sensors. The sensor values measured by the multi-inertial sensor are transmitted to the main computer through the MCU, and the attitude angle of the vehicle is finally calculated through the inverse variance weighted average. The travel distance of the robot is estimated by using hall sensors and neodymium magnets attached to the inside of the wheels. Also, we measured the pipe diameter using multiple ultrasonic sensors. We verified the estimation accuracy of each sensor through experiments and consequently estimated the 3D trajectory of the in-pipe robot.

지난 10년간 선박의 횡동요 복원력 상실에 의한 해양사고가 지속해서 증가하고 있어, 횡동요 운동을 효과적으로 줄일 수 있는 장치가 필요한 실정이다. 횡동요 감쇄 탱크는 단순한 설치만으로 횡동요 저감을 가져오는 대표적인 수동형 제어장치로 그 장점이 널리 알려져 있다. 따라서 본 연구에서는 U-튜브형 횡동요 감쇄 탱크의 수치해석 기법을 개발하고자 한다. 특히, 해석기법의 검증을 위해 자유 수면 높이를 실험을 통해 계측하였다. 수치해석기법은 메쉬 의존성, 난류모델 ( ,  , Reynolds Stress Model), 시간 간격 크기 및 반복 횟수 등의 영향을 비교하여 개발하였다. 최종적으로 개발된 해석기법은 Realizable  이 난류 모델에 10-2s 수준의 시간 간격 크기와 15 회의 반복횟수를 적용하였다. 2가지의 U-튜브형 감쇄 탱크의 조건에서 계측된 자유수면 높이를 이용하여 개발된 해석기법을 검증하였다. 본 연구의 수치해석은 RANS 기반 상용 해석 Solver인 STAR-CCM+ (ver. 17.02)을 이용하였다.

대륙이동설로부터 시작되었고 이후 고지자기 및 해저퇴적물 등의 증거들에 의한 해저확장설을 통하여 정립된 판 구조론은, 지각의 운동을 몇 개의 강체 판들이 비록 느리지만 수억 년 이상의 긴 시간 동안 꾸준히 일어나는 움직임으로 서 설명하였다. 초기에는 지각판의 속도를 주로 수백만 년 동안의 고지자기 역전의 잔류 기록에 의거하여 판들 간의 상 대적 운동으로 추산하였는데, 1980년대 이후에는 우주측지 기법들을 이용하여 현재 시점의 판운동을 직접적으로 조사할 수 있게 되었고, 일부 지역에서 판의 변형이 일어나는 것도 확인하게 되었다. 본 해설에서는 (1) 초기의 상대적인 판운동 을 나타내는 모델들을 돌아보고, (2) 무회전 좌표계의 이론과 절대판운동 모델들을 요약-기술하며, (3) 판내부의 변형을 포함하는 최근의 모델을 소개하는 한편, (4) 국제 지구 기준계에 채택된 판운동 모델을 간략히 기술하였고, 끝으로 (5) 근 래에 보고된 남미, 남극, 유럽 등 몇 지역과 (6) 한반도 및 동북아의 지각판 움직임 연구를 각각 소개하였다.

There has been increased interest in lignans due to their potential effect in reducing the risk of developing several diseases. To evaluate lignan contents, sensitive and accurate methods should be developed for their quantification in food. The present study aimed to validate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of 5 lignans: lariciresinol (Lar), matairesinol (Mat), pinoresinol (Pin), secoisolariciresinol (Seco), and syringaresinol (Syr). The validation included selectivity, linearity, recovery, accuracy, and precision. The method was proved to be specific, with a linear response (R2≥0.99). The limits of detection were 0.040~0.765 μg/100 g and the limits of quantification were 0.114~1.532 μg/100 g. Recoveries were 90.588~109.053% for black sesame powder. Relative standard deviations of repeatability and reproducibility were below 5%. Total lignan contents of roasted coffee bean, oat, and blacksoy bean were 105.702 μg/100 g, 78.965 μg/100 g, and 165.521 μg/100 g, respectively. These results showed that LC-MS/MS analysis would be effective in producing acceptable sensitivity, accuracy, and precision in five lignan analyses.

This article defines the characteristics of Korean vegetarianism by tracing the changes in the core motives revealed in the historical development of the vegetarian movement that started in the West. We further explore and compare the limitations of the vegetarian movement conceived in the West with the essential values, ‘How can Korean vegetarian culture gain the upper hand?’. Our results indicate that the sequential changes of the motives inherent to the flow of the modern vegetarian movement were <religious-philosophy>, <political-society>, and <healthy-nutrition>. This settled the transition from ‘vegetarianism as an ideological form’ to ‘vegetarianism as a lifestyle’, making it a more becoming way of life. However, along with the spread of ‘vegetarian lifestyle as a form of life’, commercial vegetarianism, which overshadows the essential value of vegetarianism, continued to flourish due to the modern capitalist industrial system. To curb commercialization, the necessity arose to establish a new vegetarian diet with a propensity towards an ‘ecological-environmental point of view’. Thus, in order to establish the term and concept of a vegetarian diet for Korean vegetarian characteristics, we propose the formation of a vegetarian term and concept suitable for the Korean vegetarian culture.

This study was conducted to identify the distribution characteristics of the impervious area in urban watersheds and to reduce the deviation of the impervious area ratio that occurs depending on the degree of construction of land surface condition data. The average impervious area ratio by land use that can be applied to the calculation of the urban impervious area ratio was derived by statistically analyzing the distribution characteristics of the impervious area ratio by land use according to the urban watershed conditions. In urban watersheds, the change in impervious area ratio over the past 20 years has continuously increased in watersheds with an impervious area ratio of less than 60%, and decreased in watersheds with a high impervious area ratio of 60% or more. The average impervious area ratio by land use applicable to the land use technique is “Residential area” 84.0%, “Residential and commercial mix” 93.6%, “Commercial and business facilities” 89.8%, “Industrial land” 84.8%, “Public land” 47.3%, “Transportation facility” 93.3%, “Urban revitalization facility” 61.1%, “Bare land” 17.6%, “Special area” 11.4%, “Forest and open space” 3.5%, “Rivers and lakes” 9.2%. As a result of examining the adequacy of the average impervious area ratio by land use, the difference between the calculated value of the impervious area ratio using land use techniques and the actual impervious area ratio of the biotope map ranged from -3.0%p to 2.6%p at the significance level of 95%. In addition, when the watershed condition is applied, the difference ranged from -2.3%p to 1.7%p. By applying the average impervious area ratio by land use derived in this study, it was found that the impervious area ratio of the target urban watershed could be calculated within a deviation of ±3%p.

본 논문에서는 휨 항복 메커니즘을 기반으로 한 강재이력 댐퍼를 제안하기 위한 해석 및 실험적 연구를 수행하였다. 댐퍼는 휨모멘 트에 의한 항복거동을 하도록 설계된 일련의 댐핑 플레이트로 구성된다. 실험 결과와 유한요소해석 결과의 비교를 통해서 본 연구에 서 채택된 해석적 접근방식이 댐핑 플레이트의 형태 및 상세에 대한 민감도 연구를 수행하기에 적절함을 확인하였다. 최초에 제안된 댐퍼는 휨 항복 메커니즘을 기반으로 작동하는 것으로 고안되었으나, 댐핑 플레이트의 인장 거동에 대한 기여도가 상당할 수 있음을 관찰할 수 있었다. 댐핑 플레이트의 두께가 증가함에 따라 휨 항복에 의한 에너지 흡수량이 증가한다. 댐핑 플레이트의 두께가 감소함 에 따라 인장에 의한 댐퍼의 비선형 거동 기여도가 증가하고 좌굴 발생으로 인하여 이력곡선의 형상이 댐퍼로서 불리해진다.

This study set up the revenue water ratio that could be achieved within the range of the expenses of the water distribution network maintenance project, developed an analysis methodology that could estimate the additional project quantity to achieve the target revenue water ratio of 85% and applied and verified that to S. City. This methodology allowed the distribution of the leakage quantity for each leakage component by the pipeline through the total revenue water account balance analysis and BABE approach and the redistribution into the calculated leakage quantity more accurately through a step test. In addition, the level of reduction in leakage and the quantity of the project were estimated before and after the application of four strategies for the promotion of the revenue water ratio presented by IWA, according to the leakage components by the pipeline. As a result of the application of this analysis method to S. City, it would be possible to achieve up to the revenue water ratio of 81.0%, which was 74.7% in the beginning, if the water distribution network maintenance project was promoted within the range of the project expenses, and to achieve the revenue water ratio of 85.0%, the goal of the project, it would be necessary to replace the pipeline of 22.2% of the entire pipelines in the target area. As a result of the re-estimation of the revenue water ratio achievable, applying the actual water distribution network maintenance quantity in the scope of the business with the results of this analysis, the revenue water ratio was 81.7% while the actually measured revenue water ratio was 82.3%. Thus, the reliability of this analysis method could be secured to some extent.

냉매 오충전은 에어컨에서 빈번하게 발생하는 고장 모드 중 하나로, 적정 충전량 대비 부족 및 과충전 모두 냉방 성능의 저하를 유 발하므로 충전된 냉매량을 정확하게 판단하는 것이 중요하다. 본 연구에서는 퍼지 군집화 기법을 통한 정상상태 식별을 통해 냉매 오 충전량을 다중 분류하는 모델을 개발하였다. 정상상태 식별을 위해 에어컨 운전 데이터에 대해 이동 평균 간의 차이를 활용한 퍼지 군 집화 알고리즘을 적용하였으며, IFDR를 통해 기존 연구된 정상상태 판단 기법들과 식별 결과를 비교하였다. 이후, 시스템 내 상관성 을 고려한 mRMR을 이용해 특징을 선택하였으며, 도출된 특징을 이용해 SVM 기반의 다중 분류 모델이 생성되었다. 제안된 방법은 시험 데이터를 통해 만족할 만한 분류 정확도와 강건성을 도출하였다.

The management of algal bloom is essential for the proper management of water supply systems and to maintain the safety of drinking water. Chlorophyll-a(Chl-a) is a commonly used indicator to represent the algal concentration. In recent years, advanced machine learning models have been increasingly used to predict Chl-a in freshwater systems. Machine learning models show good performance in various fields, while the process of model development requires considerable labor and time by experts. Automated machine learning(auto ML) is an emerging field of machine learning study. Auto ML is used to develop machine learning models while minimizing the time and labor required in the model development process. This study developed an auto ML to predict Chl-a using auto sklearn, one of most widely used open source auto ML algorithms. The model performance was compared with other two popular ensemble machine learning models, random forest(RF) and XGBoost(XGB). The model performance was evaluated using three indices, root mean squared error, root mean squared error-observation standard deviation ratio(RSR) and Nash-Sutcliffe coefficient of efficiency. The RSR of auto ML, RF, and XGB were 0.659, 0.684 and 0.638, respectively. The results shows that auto ML outperforms RF, and XGB shows better prediction performance than auto ML, while the differences between model performances were not significant. Shapley value analysis, an explainable machine learning algorithm, was used to provide quantitative interpretation about the model prediction of auto ML developed in this study. The results of this study present the possible applicability of auto ML for the prediction of water quality.

In South Korea, the history of the qualifications has been matured with industry development. Since National Technical Qualifications Act was enacted in 1973, the government makes an effort to develop the ability for improving socioeconomic status and the contribution of qualifications to industry. As the industry has been diversified and globalized, the government has efficiently and systematically operating the qualification by enacting Framework Act on Qualifications in 1997. And then, the qualifications are classified into national qualifications, private qualifications, and foreign qualifications. However, it is hard for national qualifications to abolish or establish a qualification by each organization and the privileged license acquisitors as the qualifications are separately operated by the laws and the organizations and lack consistency. For private qualifications, it is harder to organize even by the government because of the entry barriers by each qualification association. For those reasons, it is necessary to organize the system adapted in the changing job market and operated by the organization managing the qualification system with consistency. This research indicates the inefficiency of the qualification system because the work-study parallelism National qualification system, enacted in 2020, is like the qualification system in business in terms of the general operating system and the operator, except the institute and the law related. Therefore, we analyze the work-study parallelism National qualification system and the qualification system in business and suggest the efficient method to operate and the future research plan regarding the whole qualification system.