Many transcription factors are involved in directing the growth of porcine oocytes. The localization and expression level of a given transcription factor often differ at each stage of early embryonic growth, which spans from fertilization to the formation of the blastocyst. A hallmark of the blastocyst stage is the separation of the endodermal and mesodermal ectoderm. The embryo's medium and its effects are known to be crucial during early development compared to the other developmental stages, and thus require a lot of caution. Therefore, in many experiments, early development is divided into the quality of oocyte and cumulus cells and used in experiments. We thought that we were also heavily influenced by genetic reasons. Here, we examined the expression patterns of five key transcription factors (CDX2, OCT4, SOX2, NANOG, and E-CADHERIN) during porcine oocyte development whose expression patterns are controversial in the pig to the literature. Antibodies against these transcription factors were used to determine the expression and localization of them during the early development of pig embryos. These results indicate that the expressions of key transcription factors are generally similar in mouse and pig early developing embryos, but NANOG and SOX2 expression appears to show species-specific differences between pig and mouse developing embryos. This work helps us better understand how the expression patterns of transcription factors translate into developmental effects and processes, and how the expression and localization of different transcription factors can crucially impact oocyte growth and downstream developmental processes.

Chamaecyparis obtusa is one of the economical conifers planted in Korea due to its good quality timber and wood characteristics. Individuals of C. obtusa containing high terpenes (HT) and low terpenes (LT) were selected for by colorimetric method. The HT of C. obtusa was delayed in wilting against various abiotic stresses compared to the LT plants. The HT group exposed to UV did not significant influence the chlorophyll content, and the chlorophyll value was higher in the HT group than the LT group. Also, chilling treatment (5℃) did not significant influence on the chlorophyll content. However treatment at -4℃ showed relatively low chlorophyll content in the LT group than the HT group. Plants exposure to high temperature was not a difference between the HT and the LT group. However, treatment at 38℃ influenced the chlorophyll content that was increased exposure time-dependently. In salt treatments, chlorophyll in the HT group was lower at high concentrations (300 and 500 mM) of NaCl. However, chlorophyll content increased to slightly in treatment time-dependently, which is 6.7% to 40%. H2O2 treatment has been a negative effect on the chlorophyll content in the HT group. All concentration of H2O2 decreased the chlorophyll content of 5% to 35%. Plants containing high terpenoids were resisted against some abiotic stress such as salt and H2O2. Our results implied that terpenoids could cause various abiotic stress resistance. These results could be utilized for efficient management and biomass production during forest silvicultures.

Stem and root of elm trees have used as traditional medical materials, but there is little information on the distribution and resources of habitats. Korean native growing Ulmus spp. (U. davidiana var. Japonica, U. parvifolia, U. davidiana, and U. macrocarpa) genetic resources studied through The National Forest Inventory of Korea data and field survey. The distributions of U. davidiana var. japonica according to elevation distributed evenly. Both U. parvifolia and U. davidiana were inhabited mostly at less than 200 m of altitude. Each Ulmaceae species widely were distributed nationwide, but a dominant species was different depending on locals. It observed that Ulmaceae inhabits mainly in steep slopes of 31-45 degrees. Most of the habitats regenerated by natural seeding and the most abundant species were a codominant tree. Distribution of trees in U davidiana var. japonica was 7 m-13 m, and in young U. parvifolia and U. macrocarpa, more than 25% of young trees less than 7 m observed. The distribution of the diameter of breast height of the U. davidiana var. japonica was 46.4% for 11-20 cm, 52.6% for 11-20 cm in U. parvifolia. The average T/R ratio was 0.83, and the mean weight ratio of root bark was 62%. As the results of this study, the domestic Ulmaceae biomassare very small. It is difficult to harvest in that the habitat on the slope. Thus, it is too hard to develop functional materials using biomass at present. Therefore, it is necessary to develop technology for the selection and propagation of elite trees of Ulmaceae.

The Gwangneung arboretum, affiliated to the Korea Forest Research Institute, was established in 1987 and it became the Korea National Arboretum (KNA) in May 24, 1999. The entomological collection of KNA currently holds a total of 510,365 specimens of 6,849 species including more than 330 type specimens of Lepidoptera, Hemiptera, Hymenoptera, and Coleoptera are deposited. As the results of recent systematic studies by KNA entomologists, 67 new species were described, and 108 species were newly recorded from Korea and other countries. Not only we have conducted taxonomic studies but also have broaden our research boundary to natural enemy surveys for pine wilt disease vectors, plant and insect interaction, and exotic insect species monitoring. In this presentation, we would like to briefly introduce current entomological researches of KNA in the hope of future collaborations with other entomologists.

주머니나방과(Psychidae)는 곡식좀나방상과 (Tineoidea)에 속하며 전 세계적으로 241속 1,350종이 분포하는 것으로 보고되어 있다. 이들은 유충시기에 주머니를 만들고 그 속에서 사는 독특한 생활방식과 단순한 암컷의 외부형태 특징 등으로 인해 생태 및 계통학적 연구가 다양하게 시도되고 있다. 본 연구에서는 현재까지 수행된 한국산 주머니나방 과의 분류학적 연구결과를 소개하고 근연분류군에 대해 고찰하고자 한다.

This study was conducted to evaluate the efficacy of mulberry cake mixed diet on larval growth of Protaetia brevitarsis. As a result of adding mulberry cake to oak fermented sawdust and mulberry fermented sawdust, the survival rate of P. brevitarsis larvae were higher than those of the control when mass rearing larvae. When fed oak fermented sawdust with 3, 5% and 10% of mulberry cake, the cumulative proportion of larvae over 2.5g was 92.9% at 65 days, 90.8% at 55 days, and 96.3% at 45 days after rearing at 25±1℃, respectively. When fed mulberry fermented sawdust with 3%, 5% and 10% of mulberry cake, the cumulative proportion of larvae over 2.5g was 86.2% at 85 days, 90.7% at 75 days, and 91.5% at 75 days after rearing at 25±1℃, respectively.

This study was conducted to evaluate the efficacy of mulberry leaf mixed diet on larval growth of Protaetia brevitarsis. As a result of adding mulberry leaves to oak fermented sawdust, the survival rate of P. brevitarsis larvae were higher than those of the control when mass rearing larvae. When fed oak fermented sawdust with 5%, 10% and 20% of mulberry leaves, the cumulative proportion of larvae over 2.5g was 93.1% at 65 days, 95.6% at 55 days, and 93.9% at 55 days after rearing at 25±1℃, respectively. Also, heavy metals were not detected in larvae of P. brevitarsis fed on mulberry leaves.

전라북도는 곤충사육농가의 77%인 121농가가 흰점박이꽃무지를 사육하고 있고 농가수의 증가가 예상됨에 따라, 생산단가를 절감시키면서 효율적인 사육을 위해서는 사육환경을 최적으로 유지하는 것이 매우 중요하다. 따라서 본 연구에서는 뽕나무발효톱밥을 이용하여 유충사육밀도에 따른 유충발육상황을 조사하였다. 최적 사육밀도 설정을 위하여 전라북도 사육농가에서 가장 많이 사용하는 20L 크기의 사육 상자에 각각 100, 125, 150, 175, 200마리 흰점박이꽃무지 유충을 넣고 뽕나무발효톱밥을 2/3정도 채운 후 사육하면서 유충무게변화, 생존율 등을 조사하였다. 부화 후 95일 흰점박이꽃무지 유충 생존율은 평균 81%로 사육밀도별 큰 차이가 없었다. 유충 무게는 100마리/20L 사육 시 2.219g로 가장 높게 나타났으며, 사육밀도가 증가함에 따라 감소하는 경향으로 나타났다. 유충 영양성분 조사결과 단백질 52.8g/100g, 지방 11.8g/100g이었으며, 중금속은 검출되지 않았다.

Here, we investigated antioxidant defense mechanism in the spermatheca of A. mellifera queens via RNA-seq analysis of spermathecae in both mated and virgin queens. We identified the genes encoding antioxidant proteins, which were differentially expressed in the spermatheca of mated queens. The concentrations of antioxidant proteins, such as superoxide dismutase 1 (SOD1), catalase, glutathione peroxidase (GTPX), and transferrin (Tf) together with the levels of ROS, H2O2, and iron were higher in the spermathecal fluid of mated queens as opposed to those in the spermathecal fluid of virgin queens; this indicated that increase in antioxidant protein concentration is an antioxidant defense mechanism occurring in the spermathecal fluid of mated queens against ROS; this mechanism involves conversion of ROS using antioxidant enzymes and Tf-mediated inhibition of the Fenton reaction occurring between Fe2+ and H2O2. Our data indicate that an increased expression of antioxidant proteins could facilitate prolonged storage and survival of sperms in the spermatheca of mated queens, suggesting the role of antioxidant proteins in antioxidative defense against ROS.

The periodic occurrence of biological population is affected by several biological and environmental factors and ancient literature survey offers insight into ecological process related to the cyclicity. The periodic occurrence of a Lepidopteran species, the pine caterpillar (Dendrolimus spectabilis), was estimated based on ancient literature survey. The pine caterpillar was one of the most injurious defoliators of pine trees in northeast Asia, especially Korea during 1960s and 1970s. Outbreaks of this species have long history in Korea. Since about 900 years ago, damages of pine forest by this species and its control were recorded both in History of Corea dynasty which cover 475 years (918-1392) of the history of the Corea Dynasty and in the Annals of Joseon Dynasty (Joseon Wangjo Shillok) which cover 472 years (1392-1863) of the history of the Joseon Dynasty, respectively. At least over 20 and 30 occurrences of pine caterpillar were recorded during Corea dynasty and Joseon Dynasty, respectively. These results showed that the pine caterpillar had been one of the most severe forest pests during Corea and Joseon Dynasty. Our result showed that the ancient literature survey is useful to understand the long-term periodic occurrence of the pine caterpillar.

최근 기후변화와 더불어 사과원 병해충 방제 횟수, 주변 식생 등의 변화로 인해 노린재류의 발생 밀도가 증가하고 피해가 점점 늘어나고 있다. 사과원 노린재류의 방제는 주로 살충제를 이용하고 있으나 이들의 강한 이동성과 농약 잔류, 작업자에 대한 농약 노출 등의 문제를 야기하는 단점이 있어 이러한 문제를 해결할 수 있는 새로운 해충 방제 수단이 필요한 실정이다. 본 연구는 사과원의 주요 노린재 중 썩덩나무노린재와 갈색날개노린재의 알을 이용하여 사과원 주변의 알 기생봉을 탐색하고 이들에 대한 야외 기생률을 알아보고자 실시하였다. 저온 저장된 두 종의 알을 사과원에 배치하고 이후 수거하여 알에서 채집된 기생봉을 동정한 결과 썩덩큰검정알벌(Trissolcus japonicus Ashmead)로 확인되었다. 야외 기생률은 썩덩나무노린재의 경우 약 27%, 갈색날개노린재는 21%로 나타났다. 추후 이 기생봉에 대한 생태 특성, 인공 사육, 야외 방사 기술 등의 생물적 방제제로 활용하기 위한 다양한 연구를 진행할 예정이다.

Background: There have been many studies on self-myofascial release (SMR) stretching, but there are few comparative studies on the effects of massages using a release ball, which is a type of the SMR method. Objective: To investigate the immediate effects of release ball massage and self- stretching on proprioceptive sensory, hamstring’s temperature, range of motion (ROM) muscle strength,. Design: Crossover study. Methods: Thirty women in 20’s at S University in Busan voluntarily participated in the study. Participants were random to release ball group (n=15) or selfstretching group (n=15). Both groups performed 3 sets of exercises, stretching for 30 seconds and resting for 15 seconds in each position. The proprioceptive sensory, temperature of the hamstring muscle, ROM, and strength were measured before exercise, 5 minutes after exercise, and 30 minutes after exercise. Results: Release ball group showed significant differences in muscle length and temperature over time (p<.05). The comparison between two group over time showed significant differences in muscle length, temperature, and muscle strength (p<.05). Conclusions: These results demonstrate that release ball massage and selfstretching are beneficial for improving hamstring's temperature, ROM and muscle strength.

고온기 화색발현이 우수하고 연중생산이 가능한 수출용 스프레이국화 신품종을 육성하기 위하여 충남농업기술원 화훼 연구소에서 2010년 분홍색의 모본 ‘Borami’를 방임수분하였다. 2011년에 종자를 파종하였고, 이중 화형과 화색이 우수한 개체를 선발하여 ‘SP11-148-01’로 계통명을 부여하였다. 2011년부터 2013년까지 주년 생산성을 위해 전조, 자연, 차광재배로 특성을 각각 검정하였고, 생육 및 개화특성은 화형과 화색이 비슷한 자주색 스프레이국화인 ‘Kingfisher’를 대조품종으로 조사하였으며, 2013년 ‘Yes Ruby’로 품종등록 출원하였다. ‘Yes Ruby’는 자주색의 설상화와 연녹색의 통상화로 가을 작형 개화기는 10월 24일로, ‘Kingfisher’의 10월 29일에 비해 빨랐다. ‘Yes Ruby’의 초장과 줄기굵기는 각각 94.9cm와 7.7mm로 ‘Kingfisher’의 89.2cm와 6.4mm보다 컸다. ‘Yes Ruby’의 꽃 직경은 6.2cm로 ‘Kingfisher’의 5.0cm보다 컸으며, 꽃잎수도 ‘Yes Ruby’가 25.7개로 ‘Kingfisher’의 23.3개보다 많았다. 착화수는 두 품종 모두 비슷하였으며, 흰녹병 저항성은 ‘Yes Ruby’가 2단계, ‘Kingfisher’는 3단계의 감염 정도를 나타내어 흰녹병에 대한 저항성이 높은 것으로 나타났다. 재배상 유의사항은 ‘Yes Ruby’는 겨울철 균일한 개화가 이뤄지지 않아 겨울철 야간온도를 18℃ 이상으로 관리해줌으로써 균일개화를 유도할 수 있다. 또한 생장억제제인 Daminozide을 처리함으로써 설상화수를 늘려 볼륨감 높은 꽃봉오리를 형성할 수 있어 고온기에도 화색발현이 우수하고 연중생산이 가능하여 안정적 수출을 통한 농가소득 증대에 기여할 수 있을 것으로 기대된다.

Photosynthetic characteristics and growth responses of Phalaenopsis Queen Beer ‘Mantefon’ orchid were determined in plants exposed to variable carbon dioxide (CO2) concentrations at 2-, 24-, and 36-weeks age (i.e., corresponding to juvenile, young, and mature vegetative growth stages, respectively). Plants were grown at 400 (control), 800, or 1,600 μmol･mol-1 CO2 for 6 hours during the nighttime for 32 weeks. Phalaenopsis ‘Mantefon’ in 2- and 24-week-old plants grown at 1,600 μmol･mol-1 CO2 had increased leaf number and net CO2 uptake compared with the plants grown at 400 μmol･mol-1 CO2. In 36-week-old of Phalaenopsis ‘Mantefon’, leaf number was significantly greater in plant grown at 800 and 1,600 μmol･mol-1 conditions compared with plants grown at 400 μmol･mol-1 CO2. Leaves that emerged after the start of the CO2 treatment were initially longer in the plants grown at 1,600 μmol･mol-1 CO2 than at 400 μmol·mol-1 C O2, but the final leaf length was shortest in the plants grown at 1,600 μmol･mol-1 CO2 condition. Plants showed crassulancean acid metabolism characteristic of nighttime CO2 uptake regardless plant growth stages. We found that growers may be able to promote leaf growth with increasing leaf number and reducing time to leaf initiation in the 36-week-old (i.e., mature stage) plants with 800 – 1,600 μmol·mol-1 CO2 and 2- and 24-week-old (i.e., juvenile and young stages) plants with 1,600 μmol·mol-1 C O2 for Phalaenopsis ‘Mantefon’.

Recently, the importance of on-site detection of pathogens has drawn attention in the field of molecular diagnostics. Unlike in a laboratory environment, on-site detection of pathogens is performed under limited resources. In this study, we tried to optimize the experimental conditions for on-site detection of pathogens using a combination of ultra-fast convection polymerase chain reaction (cPCR), which does not require regular electricity, and nucleic acid lateral flow (NALF) immunoassay. Salmonella species was used as the model pathogen. DNA was amplified within 21 minutes (equivalent to 30 cycles of polymerase chain reaction) using ultra-fast cPCR, and the amplified DNA was detected within approximately 5 minutes using NALF immunoassay with nucleic acid detection (NAD) cassettes. In order to avoid false-positive results with NAD cassettes, we reduced the primer concentration or ultra-fast cPCR run time. For singleplex ultra-fast cPCR, the primer concentration needed to be lowered to 3 μM or the run time needed to be reduced to 14 minutes. For duplex ultra-fast cPCR, 2 μM of each primer set needed to be used or the run time needed to be reduced to 14 minutes. Under the conditions optimized in this study, the combination of ultra-fast cPCR and NALF immunoassay can be applied to on-site detection of pathogens. The combination can be easily applied to the detection of oral pathogens.

본 연구를 통해 잔디주머니나방속이 국내에서 처음 발견되어 보고한다. 잔디주머니나방속내 잔디주머니나방에 대한 성충, 유충, 번데기 등 형태학적 특징을 재기재하였다. 또한 신속한 종 동정을 위한 DNA바코드 정보를 제공하였다.

기관지담관루는 간세포암 환자에서 여러 차례의 간동맥화학색전술 후 드물게 일어날 수 있는 합병증이다. 본 증례의 78세 남자 환자는 11차례의 간동맥화학색전술을 시행받고 5개월 후 발생한 기침과 담즙성 객담으로 내원하였다. MRCP상 기관지담관루는 오른쪽 간내담관(B7)과 기관지 사이에 위치하였다. ERCP를 시도하였으나 심한 간문부의 폐쇄로 기관지담관루가 위치한 우측 간내담관을 조영할 수 없었고, 이후 PTBD를 시행하여 매일 30-50 mL의 담즙이 배출되면서 객담 배출은 멈추었다. PTBD 배액관을 유지하고 2개월 동안 관찰하였으나 누공이 지속되어 두 번째 ERCP를 시행하여 기관지담관루 부위에 플라스틱 스텐트를 유치하였다. 1주 후 PTBD를 통하여 시행한 담도조영술에서 기관지담관루는 소실되었다. 환자는 스텐트 시술 이후에 더 이상 객담을 배출하지 않았고 2개월 이후에 시행한 ERCP에서도 기관지담관루가 관찰되지 않았다.