The decommissioning of nuclear power plant (NPP) generates large amount of waste. Since the most of the concretes are slightly surface contaminated, the accurate characterization and regionspecific surface decontamination are important for the efficient waste management. After the effective surface decontamination and separation, most of the concrete waste from decommissioning of NPP can be classified as a clearance waste. Various surface characterization and decontamination technologies are suggested. The mechanical technologies are simple and offers direct application. The laser-based technologies offer efficient separation and surface contamination. The high price, however, hesitates the application of the process. The nitro-jet technology, which is based on the evaporation of liquid nitrogen, allows the effective decontamination. However, the high price and uncertainty of large are application hinders the practical application in NPP decommissioning. In this paper, various technologies for characterization, handling, treatment, etc., will be discussed. The advantages and disadvantages of the technologies will be discussed, in terms of practical applications.

For transport containers for radioactive wastes, a drop test should be performed at a height of 0.3– 1.2 m on a rigid target depending on the weight as a normal condition in the regulation. In the drop test, a strain gauge is commonly used to measure the local strain, and the position of the strain gauges is determined by the experiences of the engineer in advance of the test. For this reason, the strains can be measured at only predetermined points. The DIC (Digital Image Correlation) method using highspeed cameras can be used to measure the change in strain over the region of interest. In addition, it is possible to measure effectively even in areas with high strain gradients that are difficult to measure with strain gauges. Therefore, the DIC method can measure the strain change according to time over the entire load path. When the drop test of the transport container is performed, the impact load is delivered through the lower corner fittings-corner posts-upper corner fittings-lids. In this study, white spray was sprayed on these main load path, and black speckles were created on the spayed surface to trace the rigid motion of speckles. The images taken during the drop test can be used to create a strain field over region of interest.

In this study, a drop analysis of metallic disposal containers for radioactive wastes is performed according to accident scenarios at the disposal site. The weight of the disposal container is about 8 tons, and the ingot-type wastes are loaded in the disposal container. To simulate the floor of the disposal site as the impact target, the reinforced concrete pad is modeled. High impact energy of the disposal container due to their heavy weight and high drop height causes excessive deformation and failure of the concrete target having relatively weak strength. Dynamic growth of cracks due to such failures causes penetration and delamination of concrete. Since the impact force delivered to the container strongly depends on the failure of the concrete pad, it is important to properly simulate the failure of the concrete in the drop analysis. A material erosion method can be used to simulate the concrete failure. In the case of applying erosion based on the finite element method (FEM), the element is deleted when the element exceeds a certain criterion, which causes material and energy loss problem. To solve this problem, mesh-free methods such as smoothed particle hydrodynamics (SPH) can be commonly used, but the mesh-free method has the disadvantage of incurring high numerical cost. Therefore, an adaptive method combining SPH and FEM-based SOLID elements is used for concrete target modeling to simulate excessive deformation and failure of the concrete target. In the adaptive coupling method of SPH and SOLID, the concrete target is first modeled as a solid element. When the damage of concrete exceeds the failure criterion, the solid element is eroded and the SPH element replacing the solid element is activated. Since the activated SPH element continues to participate in the impact, the problem of loss of materials and energy can be effectively solved. In this way, analysis results consistent with actual physical phenomena can be obtained.

The bacterial soft-rot disease is one of the most critical diseases in vegetables such as Chinese cabbage. The researchers isolated two bacteria (Pseudomonas kribbensis and Pantoea vagans) from diseased tissue samples of Chinese cabbages and confirmed them as being the strains that cause soft-rot disease. Lactic-acid bacteria (LAB), were screened and used to control soft-rot disease bacteria. The researchers tested the treatments with hypochlorous acid water (HAW) and LAB supernatant to control soft-rot disease bacteria. The tests confirmed that treatments with the HAW (over 120 ppm) or LAB (Lactobacillus plantarum PL203) culture supernatants (0.5 mL) completely controlled both P. kribbensis and P. vagans.

본 연구에서는 국내산 돌미나리에서 GABA생성능이 있 는 신규 유산균을 분리, 동정한 결과, Enteroccoccus casseliflavus로 확인되었다. 최근까지 Lactobacillus속과 같 은 GABA 생성 유산균에 대한 보고는 많이 되고 있고, 일 부 Enterococcs속 유산균도 보고되고 있으나 E. casseliflavus 종에 대한 보고는 없었다. 따라서 본 연구에서는 E. casseliflavus PL05 균주에 대한 GABA 생성 최적 조건을 찾기 위하여 배지의 유형, 생육 온도, 초기 pH 조건, 배양 시간, MGS 농도 및 탄소원을 포함한 다양한 조건을 테스 트하였다. PL05 균주는 MRS 혹은 TSB 배지보다 BHI 배 지에서 생육이 잘 되었으며, 배지의 초기 pH는 7-9 조건 에서 가장 생육이 왕성하였고, GABA 생성 조건 역시 유 사한 결과로 확인되었다. GABA의 기질에 해당하는 MSG 의 농도별 GABA 생성량을 조사한 결과, 7%에서 가장 높 은 생성량을 나타내었으나 5%에서도 유사한 수준으로 확 인되어 효율적인 측면에서 5%가 적합할 것으로 판단된다. 탄소원에 따른 생육 및 GABA 생성량은 말토오스를 사용 하였을 때 가장 높은 것으로 확인되었고, 이러한 최적의 조건들로 최종 테스트를 진행한 결과, 24시간째 140.06±0.71 mM의 GABA가 생성되었고, 전환율은 78.95%로 확인되 었다. 또한 반코마이신을 포함한 10개의 항생제에 대한 감 수성을 조사한 결과 내성이 없는 것을 확인하였다.

Colorectal cancer causes the most cancer-associated death worldwide, having a high cancer incidence. Pectin is a complex polysaccharide present in various fruits, emerging as an anti-carcinogenic candidate. Although pectin has a suppressive capacity for colon carcinogenesis, the effect of reactive oxygen species (ROS) generation and colonic aberrant foci formation in the colon carcinogenesis mouse model remains unclear. Therefore, this study investigates the regulatory effect of pectin supplementation on colon carcinogenesis induced by azoxymethane (AOM) and dextran sodium sulfate (DSS) in mice. In an animal experiment, thirty male institute for cancer research (ICR) mice were divided into two experimental groups; AOM/DSS (control group) and AOM/DSS + pectin (5% in drinking water). Furthermore, the number of aberrant crypt foci (ACF) and aberrant crypt (AC) on colonic mucosa were counted, and thiobarbituric acid-reactive substances (TBARS) assay was performed to estimate lipid peroxidation in feces. Pectin treatment significantly decreased the number of ACF and AC per colon compared with the control. Additionally, fecal TBARS level in the pectin group was significantly lower than those in the control group. Conclusively, these findings indicate that pectin-inhibited hyperplastic alteration and oxidative stress suppress colitis-associated colon carcinogenesis.

A solid-phase competition enzyme-linked immunosorbent assay (ELISA), recombinant VP2 (rVP2) protein, and monoclonal antibody (mAb) were developed for the specific and sensitive detection of porcine parvovirus (PPV) antibodies in pig sera. A total of 1,544 sera samples were collected from breeding pig farms located in the Gyeongsangbuk-do Province in the Republic of Korea. The optimal operating conditions of SC-ELISA were as follows. The concentration of rVP2 proteins coated on the wells was 4 μg/mL, the swine sera were diluted 1:2, and the HRP-conjugated PPV VP2 mAb (9A8 clone) was used at 500 ng/mL. These results suggest that the SC-rVP-ELISA assay may be a valuable alternative to the current diagnostic tools used to detect PPV-specific monoclonal antibodies and broadly monitor PPV infections in domestic pigs at different breeding stages.

Colon cancer has been considered a leading cause of cancer-associated death. Folic acid is a vitamin necessary for cellular physiological functions and cell viability. However, the association between folic acid intake and colon cancer has been examined in several prospective cohort studies are controversial. This study investigated the effects of folate intake on colon carcinogenesis and oxidative stress in an azoxymethane (AOM)/dextran sodium sulfate (DSS) institute for cancer research (ICR) mouse model. Thirty male ICR mice (5 weeks old) were divided into the control group and the experimental group supplied 0.03% folic acid via drinking water (50 mL/week/mouse) for 6 weeks. To induce colonic pre-neoplastic lesions, the animals were subcutaneously injected three times weekly with AOM (10 mg/kg body weight), followed by 2% DSS in drinking water for a week. Folic acid supplementation significantly suppressed the total number of aberrant crypt foci and aberrant crypts. Histological image data showed that folic acid supplementation attenuated neoplastic change. In addition, we measured the thiobarbituric acid reactive substances concentration of dry feces samples to identify the effect of folic acid on reactive oxygen accumulation. The folic acid supplementation group had reduced reactive oxygen species levels in dry feces compared to the control group. In conclusion, these findings indicate that folic acid suppresses colon carcinogenesis and oxidative stress in an AOM/DSS mouse model.

Saengcheonggukjang, known as Natto in Japan, is a soybean fermented food which is made from steamed soybean, Bacillus and water. Demand of Saengcheonggukjang has increased because it does not have much smell compared to Cheonggukjang. Seven varieties of Saengcheonggukjang were investigated and compared in terms of 100 seed weight, quality characteristics, hard seed rate, and water absorption rate in order to determine the suitability of Korean soybeans. In addition, each characteristic of Saengcheonggukjang was compared. ‘Hoseo’ and ‘Haewon’ showed low 100 seed weight with 8.41 g and 8.11 g, respectively. The water absorption rate was higher in ‘Hoseo’ and ‘Pungwon’ than in Japan varieties. The yield of Saengcheonggukjang was significantly different for each variety. No differences were observed in yield and hardness of Saengcheonggukjang with respect to the varieties and sowing date. ‘Haewon’ showed the highest amino nitrogen content with 575.0 mg%. Viscous material content did not appear to differ between varieties. These results suggested that ‘Hoseo’ and ‘Haewon’ can be considered as suitable candidates for yield and quality of Saengcheonggukjang compared to Japan varieties.