S. maltophilia 는 그람 음성 호균성 간균으로 장기간의 입원이나 과거 항생제를 사용한 경우 감염의 위험이 증가한다. 폐렴, 요로 감염 등을 흔히 유발하지만, 담도 감염은 매우 드물다. 입원 환자에서 동정이 증가되고 있으며, 자주 사용되는 항생제에 내재 내성을 가지거나 면역이 억제된 환자에서 감염을 일으키기 때문에 임상적으로 중요하다. 저자들은 만 성 B형 간염에 의한 간경변과 담낭 결석 환자에서 B형 간염에 의한 급성 간부전으로 치료하던 과정에서 발생한 S. maltophilia 균혈증에 의한 급성 담낭염 1예를 경험하여 문헌고찰과 함께 보고한다.

Conservation biological control (CBC) is one of the pest management tactics based on protecting and maintaining natural enemies that already exist in the crop environment. Among many appropriate practices to control pests, CBC has been considered as a sustainable means and a less expensive alternatives to chemicals. It contains an introduction of non-crop plants as shelter habitat for providing natural enemies with food sources, overwintering or refuges shelters. As a small scale experiment of CBC, we tried to intercrop six kinds of floral plants (buckwheat, red clover, Agastache rugosa, Chrysanthemum indicum, Allium tuberosum, Lythrum anceps) with napa cabbage in order to attract some natural enemies, especially parasitic wasps that could be fed on them in the cabbage fields, which is a novel way for conservation of natural enemies, but none showed significant result. For the development of integrated pest management system by harmonizing biological and chemical control, we carried out evaluating toxicity of 30 pesticides to parasitoids and investigating effectiveness of traps. With leaf dipping method for adult and body dipping method for cocoons, 12 pesticides showed under 30% of toxicity being selected as safer by IOBC. Using sex-pheromone traps for attracting lepidopteran pests showed that DBM, CAW and CL occurred less than untreated fields; 67.5%, 70.6% and 44.0%, respectively. Also, yellow sticky traps could reduce some pests; 52% for flea beetle, 62% for cabbage sawfly and 41% for Phaedon brassicae. These results are expected to give basic information to develop conservation biological control of DBM with indigenous parasitoids in the cabbage fields on a large scale in the future. Before developing and distributing a promising pest control method, we should consider whether it can be compatible with other agricultural practices or various situations around fields.

본 연구는 반추동물의 조사료 자원으로서 거대억새를 개발하기 위한 목적으로 수행되었다. 우리나라에서 새롭게 개발된 품종인 거대억새 1호를 완숙기 이후에 채취하여 in vitro 반추위 발효를 이용해반추위내 pH, 암모니아태 질소, 가스발생량, 휘발성 지방산 생성량 및 건물소화율을 조사하였으며,볏짚과 비교하여 평가하였다. 거대억새는 볏짚에 비하여 유의적으로 높은 반추위내 pH를 나타내었다(p<0.01). 암모니아태 질소의 경우 배양 12시간 이후에는 두 처리구간의 유의적인 차이를 나타내지 않았다 (p>0.05). 배양 6시간 이후 부터는 거대억새의 가스발생량이 볏짚에 비하여 유의적으로 낮게 나타났다 (p<0.05). 휘발성 지방산 생성량에 있어 acetate, propionate, butyrate, valerate 및 총생상량에서 볏짚이 거대억새보다 높게 나타났다. 그러나 iso-butyrate와 iso-valerate에서는 두 조사료원별 차이는 발견되지 않았다. 건물소화율에 있어 배양 12~24시간 사이의 거대억새 소화율이 볏짚에 비하여 유의적으로 나타났다. 결론적으로 거대억새의 이용성은 볏짚의 약 80% 수준인 것으로 나타났다.

The purpose of this study was to analyze lower limb muscle activity and 3D motion analysis according to change foot arch height during walking. We selected 9 young and healthy people who have been normal foot. And we selected 7 young and healthy people who have been flatfoot. So, people were divided into 2 groups and walked platform during 2 minutes twice for checked by 3D motion analysis. These data were characterized by EMG measurements of three muscles( tibialis anterior, medial and lateral gastrocnemius) while they were walking. The collected data were analyzed by Independent t test using the SPSS statistics program(Ver 12.0). In foot arch change, there were no significant difference in three muscles 3D motion analysis also found that there were no significant difference in joint angles. In this study was to analyze lower limb muscle activity and 3D motion analysis according to change foot arch, but there were no significant difference in 6 muscles neither joint angles.

This study was to examine on the respiratory variables, heart rate and muscle activity between the static recovery and dynamic recovery after progressive resistance exercise to maximal point. Subjects were 15 students enrolled in N University. All were tested two times (static recovery and dynamic recovery) and were requested to perform a walking on a treadmill after progressive resistance exercise to maximal point. Electromyography(EMG) was used to monitor the muscle activity(TA: Tibialis Anterior, MG: Medial Gastrocnemius) during gait. CPEX-1 was used to measure the respiratory variables and heart rate. The dynamic recovery group was shown the significant lower heart rate than that of static recovery group at during gait. Respiratory rate showed statistically a significant difference. Electromyography(RMS, root mean square) showed a non-significant difference. But the dynamic recovery group of muscle activity was found highly in TA and MG. This study indicated that the dynamic recovery method evidenced more faster than the static recovery method. And this type of dynamic rest by walking can be a help of recovery after exercise.

근접방사선치료는 일반적으로 외부방사선치료와 병행하여 수행되고 치료단계가 매우 복잡하며 이로 인 해 방사선 사고가 발생될 수 있다. 본 연구에서는 이를 해결하기 위해 근접방사선치료에 사고유형과 영향 분석(Failure mode and effects analysis, FMEA) 방법을 적용하여 프로세스 맵을 구성하고 이를 기반으로 각 치료단계에 대한 위해도를 산출하였다. 프로세스 맵은 “외래 및 진료”와 “근접방사선 모의치료”, “CT 모의 치료”, “근접방사선치료계획”, “방사선치료”로 총 5단계로 구성하였으며, 각 치료단계를 세분화하여 세부단 계를 작성하였다. 위해도를 산출하기 위해 의사와 의학물리사, 선량설계사, 방사선사, 간호사가 참여하여 세부단계마다 발생빈도와 심각도, 불검출도를 평가하였다. 전반적으로 프로세스 맵은 각 치료단계마다 환 자 신원 확인 절차가 우선적으로 수행되며, 이는 다른 환자로 오인하여 서로 다른 치료계획이 수립되어 방 사선사고가 발생될 우려가 있다. 프로세스 맵을 기반으로 작성한 세부단계에 대해 위해도를 평가한 결과, 전반적으로 “외래 및 진료”와 “근접방사선치료계획” 과정이 높은 위해도로 평가되었다. 직종마다 평가한 위해도는 서로 다른 경향을 보였으며, 간호사는 방사선치료를 제외한 모든 과정이 55점 이상의 위해도를 보였으며, “근접방사선 모의치료” 과정이 88.8점으로 가장 높았다. 방사선치료를 수행하는 의료기관마다 치 료단계가 다소 차이가 있으므로 해당 기관에 대한 프로세스 맵을 작성하고 위해도를 산출하여 중점관리 항목을 집중적으로 리스크 관리가 수행되어야 할 것으로 생각된다.

Background : Undulatum Rhubarb, commonly produced in domestic, is rhizome of Rheum undulatum L. that belongs to the family Polygonaceae. It also can be used as a substitute of R. palmatum L., R. tanguticum Maximowicz ex Balf., and R. officinale Baillon which completely depend on import system. However, there should be clear clarification among Undulatum Rhubarb and Rhubarb, because Undulatum Rhubarb contains rhaponticin as marker compound that is not indicated at Rhubarb. Some of the recently imported Undulatum Rhubarbs have been found to be Rhubarb. Also, it is known that only Undulatum Rhubarb is cultivated at domestic environment. But some plant origins of Rhubarb are grown in Korea, too. Further study are needed to clarify clear origin between Undulatum Rhubarb and Rhubarb. Thus, we collected some domestically cultivated samples and identified them. Methods and Reseults : Rheum undulatum L., Rhubarb, Rheum tanguticum Maximowicz ex Balf. which were cultivated in Gangwondo Agricultural Research and Extension Services in Cheorwon were collected and anayzed the DNA sequences. We also compared DNA sequences in Rhubarb collected from England and R. rhabarbarum L., R. undulatum L., and R. franzenbachii on NCBI. As a result, two kinds of rhubarb cultivated in the test plantation were identified as R. rhabarbarum and R. officinale. In addition, R. undulatum (plant origin of Undulatum Rhubarb) was identified as Rhubarb (Rheum rhabarbarum) in England with 99 - 100% identical in nuclear ITS gene region. Conclusion : R. undulatum, plant origin of Undulatum Rhubarb, is reported as synonym of R. rhabarbarum, R. franzenbachii. Rheum speices which are cultivated as tester in Gangwondo Agricultural Research and Extension Services in Cheorwon are estimated as R. undulatum and R. officinale. Therefore, not only Undulatum Rhubarb but Rhubarb could be grown in Korea.

Human embryonic stem cells (hESCs) have been routinely cultured on mouse embryonic fibroblast feeder layers with a medium containing animal materials. For clinical application of hESCs, animal-derived products from the animal feeder cells, animal substrates such as gelatin or Matrigel and animal serum are strictly to be eliminated in the culture system. In this study, we performed that SNUhES32 and H1 were cultured on human amniotic fluid cells (hAFCs) with KOSR XenoFree and a humanized substrate. All of hESCs were relatively well propagated on hAFCs feeders with xeno-free conditions and they expressed pluripotent stem cell markers, alkaline phosphatase, SSEA-4, TRA1-60, TRA1-81, Oct-4, and Nanog like hESCs cultured on STO or human foreskin fibroblast feeders. In addition, we observed the expression of nonhuman N-glycolylneuraminic acid (Neu5GC) molecules by flow cytometry, which was xenotransplantation components of contamination in hESCs cultured on animal feeder conditions, was not detected in this xeno-free condition. In conclusion, SNUhES32 and H1 could be maintained on hAFCs for humanized culture conditions, therefore, we suggested that new xenofree conditions for clinical grade hESCs culture will be useful data in future clinical studies.

With the object of examining the effects of horticultural therapy based on structural recall by developmental stage on cognitive, emotional, and hand functions of institutionalized elderly, this study executed a horticultural therapy program once a week and through a total of 14 sessions during the period from May 2 to December 5, 2013. The subjects of this study were 34 elders admitted to S Geriatric hospital for geriatric diseases, and they were divided into a control group and an experimental group. Because the program was designed for institutionalized elders who were able to do only indoor activities, it provided various seasonal horticultural activities that could be done even in a limited space by giving materials and subjects that could be recalled by developmental stage. The results of this study are as follows. As to changes in cognitive functions (MSQ) after the horticultural therapy program, the control group showed a decrease as having more negative ranks, and the experimental group showed an increase as having more positive ranks, but such changes in the control and experimental groups were not significant. As to changes in emotional functions after the horticultural therapy program, the control group showed a decrease but without significance in ego integrity as having more negative ranks, and the experimental group showed an increase with significance as having more positive ranks (P=0.020). As to the experimental group’s changes in the sub factors of ego integrity, positive ranks increased in all the sub-factors and the increase was particularly significant in satisfaction with present life (P=0.038). As to changes in life satisfaction, the control group showed increased positive ranks but without significance, and the experimental group showed significantly increased positive ranks (P=0.022). As to the experimental group’s changes in the sub-factors of life satisfaction, changes in denial of the past (P=0.029) and denial of the future (P=0.016) were particularly significant. As to changes in hand functions, both the control and experimental groups showed some improvement in pinch after the horticultural therapy program but the changes were not significant. Grip strength did not change in the control group, and in the experimental group right-hand grip strength was improved slightly more than left-hand one, the difference was not significant. The results of this study suggest that horticultural therapy based on structural recall by developmental stage is effective in improving institutionalized elders’ emotional functions and, consequently, can contribute to higher quality of life, which is the ultimate goal of elders.

A 63-year-old woman with a history of nephrolithiasis and long-term hemodialysis (HD) presented with abdominal pain. Abdominal computed tomography (CT) showed urinoma induced by renal forniceal rupture, and three causative calculi. No active stone growth or new stones were observed when compared with previous CT, however, fragmentation and migration of an existing stone was observed. After ureteroscopic lithotripsy and stenting with a double-J catheter, the patient made a full recovery. To the best of our knowledge, this is the first case of spontaneous renal forniceal rupture in an anuric HD patient caused by calculi. Renal forniceal rupture can occur, even in anuric HD patients.

Gene expressions of cytochrome P4501A (CYP1A), aryl hydrocarbon receptor (AhR) and vitellogenin (Vg) by endocrine disruptors, benzo[]pyrene (B[a]P) and tributyltin (TBT) were examined in cultured eel hepatocytes which were isolated from eels treated previously with B[a]P (10 mg/kg) or estradiol- (20 mg/kg) in vivo, and the relationship between CYP1A, AhR and Vg genes were studied. When the cultured eel hepatocytes were treated with B[a]P () the gene expressions of CYP1A and AhR were enhanced in a concentration-dependent manner. However, when treated with TBT () the gene expressions of CYP1A and AhR were suppressed at high concentrations (), while having no effects at low concentrations (). Gene expression of Vg was also suppressed by TBT in a concentration-dependent manner in cultured eel hepatocytes which was previously treated in vivo with estradiol-.

To produce abiotic stress resistant transgenic cucumber, the cotyledonary node explants of cucumber (c.v. Eunsung) were inoculated with A. tumefaciens strain EHA105 containing the binary vector (pPZP211) carrying Nit gene. The 491 explants inoculated with bacterium solution for 30 min were maintained on 50 mg/L paromomycin contained shoot induction (SI) medium for first 2 weeks and then subcultured on 100 mg/L paromomycin to obtain transgenic adventitious shoots for 4 x 14 days. So far, 5 plant were selected, and then acclimated in soil. Of them, 3 transgenic plants with Nit gene were confirmed by Southern blot analysis.

Human embryonic stem cells (hESCs) have the potential for use in regenerative medicine and in the field of basic research. Therefore, effective cryopreservation and storage of hESCs are important for preservation of newly established cell line for various purposes. Despite poor survival and slow recovery after thawing, the conventional slow freezing method is most commonly used for cryopreservation of hESCs due to its simplicity and ease of use for freezing a large number of hESCs appropriate to clinical applications. Here we controlled the clump size (Group Ⅰ; 400～450 ㎛, Group Ⅱ; 800～900 ㎛, and Group Ⅲ; 1500～1700 ㎛) of hESCs at 5 days after plating using a glass pipette during cryopreservation in order to obtain a larger amount of hESCs after thawing. Attachment rates differed significantly (P<0.05) in each of the three groups and the average of attachment rate of GroupⅡ was highest in SNUhES4 and H1. In particular, the attachment rate of Group Ⅱ in SNUhES3 showed a significant improvement with ROCK inhibitor Y-27632. These results indicate that clump size and cell-cell adhesions of GroupⅡ are appropriate for cryopreservation compared to the Group Ⅰ and Group Ⅲ. This method increased cell viability and reduced the recovery time leading to various experiments, and therefore has an advantage for use with hESCs like newly established in particular. We demonstrated that use of this effective cryopreservation method with control of the clump size of hESCs can effectively improve the attachment rate and survival of post-thaw hESCs with and without Y-27632.