Quarantine Pest Research Facility (QPRF) had been built in Animal and Plant Quarantine Agency for study on exotic pests at 2018. This facility was constructed to prevent effective containment for preventing accidental escape of insects. We tested a developmental and susceptibility of pesticides (Fluxametamide, FLX and Hydramethylnon, HMT) on beet armyworm, Spodoptera exigua for testing containment level of QPRF. S. exigua showed 22.5 days at 28±1℃ and 26.5 days at 25±1℃ with 60±5% RH for entire life cycle. LC50 of FLX and HMT to 5th instar larvae for 24h revealed as lower than 1ppm and 100ppm, respectively. Also, there is no release of adults in QPRF and confirmed by sex pheromone trap during winter season.

토양에 활용되는 유기농업자재(퇴비, 유황)가 굼벵이 생육 및 생존에 미치는 영향을 알아보기 위하여 생육시험을 수행하였다. 먹이는 참나무발효톱밥을 이용하였으며, 가축의 퇴비와 유황을 일정비율(0.3, 1, 3, 10 %) 섞어 사육하였다. 시험곤충은 흰점박이꽃무지 2령 유충을 사용하였다. 사육 시 나타나는 생장율 차이를 비교하여 흰점박이꽃무지 유충의 발육 특성을 조사하였다. 참나무발효톱밥으로만 사육한 개체에 비해 유황을 혼합한 경우, 유황의 비율이 높아질수록 생중량의 증가율이 낮아지는 것으로 나타났다. 가축퇴비(우분 40%, 돈분 10%, 계분 5%)를 배합하여 사육한 경우, 참나무발효톱밥에 10% 퇴비를 배합하였을 때, 21일 후 생중량이 평균 4.18배 증가하는 것을 확인하였다.

소나무재선충의 매개충인 솔수염하늘소(Monochamus alternatus Hope)와 북방수염하늘소(Monochamus saltuarius Gebler) 암수 성충의 더듬이 감각기를 주사전자현미경로 관찰하여 형태적 특성과 분포를 조사하였다. 두 매개충의 암수 성충 더듬이는 자루마디(scape), 팔굽마디(pedicel)와 9개의 채찍마디(flagellum)으로 이루어져 있었다. 더듬이 표면에 있는 감각기의 종류에는 종 간, 암수 간에 차이가 나지 않았으며 Chaetica 3 종류, Trichodea 2 종류, Basiconica 3 종류, Coeloconica 1 종류로 구분되었다. 이 중 Basiconica 3 종류는 모두 후각 수용체, Trichodea는 미각 수용체, 나머지는 기계적 감각기로 추정되었다. 감각기의 더듬이 내 분포를 보면 전체적으로 Chaetica 감각기가 가장 많았다. Basiconica 감각기는 채찍마디에만 존재하였으며 끝으로 갈수록 수가 많아졌다. Trichodea 감각기와 Coeloconica 감각기는 각 마디별로 밀도가 일정하였다.

솔껍질깍지벌레는 남부지방의 곰솔을 가해하는 주요 해충으로 최근 그 분포범위가 확산되고 있다. 현재 솔껍질깍지 벌레의 모니터링은 난낭의 조사를 통해 이뤄지고 있으나, 이는 매우 많은 인력이 필요하며 부정확하다. 따라서 페로몬을 이용한 솔껍질깍지벌레의 모니터링이 새로운 대안으로 제시되고 있다. 하지만 이 역시 적절한 가이드라인이 없어 현장적용에 큰 어려움이 있다. 본 연구 에서는 페로몬을 이용한 솔껍질깍지벌레의 효율적인 모니터링 기법 개발을 위하여 최적 페로몬 방출농도 및 트랩설치 방법을 규명하였으며, 페로몬의 새로운 합성기법의 개발 통하여 경제적 효율성을 기하였다. 성 페로몬에 대한 수컷 솔껍질깍지벌레의 유인효과는 100 μg부터 1,600 μg 까지의 처리에서 용량의존적인 관계를 나타내었으며 이 이상으로 처리할 경우 페로몬 처리 증가에 따른 유인개체수가 증가하지 않았다. 트랩의 설치 높이를 지표면으로부터 50cm, 100cm 150 cm로 구분하여 조사한 결과 지상부로부터 50cm에 설치한 트랩에 가장 많은 개체가 포획되었다. 새로운 방법으로 합성한 페로몬 역시 기존 페로몬과 마찬가지로 페로몬 처리량과 유인 개체수 간 용량의존적인 관계가 나타났다.

Korean Native Pig (KNP) has a uniform black coat color, excellent meat quality, white colored fat, solid fat structure and good marbling. However, its growth performance is low, while the western origin Yorkshire pig has high growth performance. To take advantage of the unique performance of the two pig breeds, we raised crossbreeds (KNP × Yorkshire to make use of the heterotic effect. We then analyzed the liver transcriptome as it plays an important role in fat metabolism. We sampled at two stages: 10 weeks and at 26 weeks. The stages were chosen to correspond to the change in feeding system. A total of 16 pigs (8 from each stage) were sampled and RNA sequencing was performed. The reads were mapped to the reference genome and differential expression analysis was performed with edgeR package. A total of 324 genes were found to be significantly differentially expressed (|log2FC| > 1 & q < 0.01), out of which 180 genes were up-regulated and 144 genes were down-regulated. Principal Component Analysis (PCA) showed that the samples clustered according to stages. Functional annotation of significant DEGs (differentially expressed genes) showed that GO terms such as DNA replication, cell division, protein phosphorylation, regulation of signal transduction by p53 class mediator, ribosome, focal adhesion, DNA helicase activity, protein kinase activity etc. were enriched. KEGG pathway analysis showed that the DEGs functioned in cell cycle, Ras signaling pathway, p53 signaling pathway, MAPK signaling pathway etc. Twenty-nine transcripts were also part of the DEGs, these were predominantly Cys2His2-like fold group (C2H2) family of zinc fingers. A protein-protein interaction (PPI) network analysis showed that there were three highly interconnected clusters, suggesting an enrichment of genes with similar biological function. This study presents the first report of liver tissue specific gene regulation in a cross-bred Korean pig.

Korean horn beetles, Allomyrina dichotoma (Linnaeus, 1771) reared in many local farms are suffering from a fatal viral disease as reported in 2015, and recent sequencing analysis revealed that the virus is very closely related to Oryctes rhinoceros nudivirus. In a nationwide investigation, it was concluded that the virus can transmit vertically from an infected adult to the offspring by indirect manner, and 70 to 77% of young larvae dead in early stage were diagnosed as this virus positive. Here, we report for the establishment of on-site diagnosis method for the viral disease using loop-mediated isothermal amplification (LAMP) assay. A. dichotoma larvae were assayed for the OrNV infectivity with LAMP primers targeting OrNV_gp102 gene. To evaluate the LAMP specificity, two bacterial pathogens, Bacillus thuringiensis and Serratia marcescens, causing disease in A. dichotoma were tested along with OrNV. Only from the OrNV-infected larvae the reaction resulted positive. Also, to avoid DNA extraction process, the LAMP assay used diluted hemolymph directly and 50-fold dilution was set for diagnosis standardization and convenient on-site application for infected larvae from local farms. The LAMP diagnosis is fast and convenient for nontechnical user in the field and expected to stop this foreign virus spread all over the country.

붉은불개미는 남미가 원산인 외래해충으로써 기후변화로 인한 기온 상승과 국가간 교역량의 증가를 통해 전세계적으로 서식범위를 확장해 나가고 있다. 국내에서는 붉은불개미가 2017년 9월 부산 감만항에서 처음 발견된 이후 올해까지 4차례 추가로 발견되어 국내 침입 및 정착 가능성이 점차 증가하고 있다. 곤충에서 인슐린 신호체계는 일반적으로 산란, 생장 및 발육, 대사계, 스트레스 저항성에 관여하는 것으로 널리 알려져 있다. 본 연구에서는 붉은불개미의 인슐린에 대한 생물학적인 기능을 이해하기 위해 인슐린 수용체(insulin receptor)의 발현을 억제한 후 나타나는 생리적 변화를 관찰하였다. 그 결과 체내빙결점(supercooling point)에 영향을 미쳐 붉은불개미의 저온생리에 영향을 준다는 것을 확인할 수 있었다.

Ticks are obligatory hematophagous ectoparasites, which can transmit various pathogens including bacteria, protozoa, and viruses via salivary secretion during feeding. Understanding salivation in ticks is crucial for the development of novel methods to prevent tick-borne disease transmission. The central nervous system (synganglion) of ticks controls salivary glands via several neuropeptidergic innervation: myoinhibitory peptide (MIP), SIFamide, and elevenin. These neuropeptides are thought to be modulators of dopamine’s action controlling the salivary glands including inward fluid transport into the lumen of salivary glands acini and emptying lumen solute into salivary duct by pumping and gating. These actions are via two distinct dopamine receptors, dopamine receptor (D1) and invertebrate D1-like dopamine receptor (InvD1L), respectively.

The purpose of this study was to explore the nine components of computational thinking (CT) practices and their operational definitions from the view of science education and to develop a CT practice framework that is going to be used as a planning and assessing tool for CT practice, as it is required for students to equip with in order to become creative problem solvers in 21st century. We employed this framework into the earlier developed STEAM programs to see how it was valid and reliable. We first reviewed theoretical articles about CT from computer science and technology education field. We then proposed 9 components of CT as defined in technology education but modified operational definitions in each component from the perspective of science education. This preliminary CTPF (computational thinking practice framework) from the viewpoint of science education consisting of 9 components including data collection, data analysis, data representation, decomposing, abstraction, algorithm and procedures, automation, simulation, and parallelization. We discussed each component with operational definition to check if those components were useful in and applicable for science programs. We employed this CTPF into two different topics of STEAM programs to see if those components were observable with operational definitions. The profile of CT components within the selected STEAM programs for this study showed one sequential spectrum covering from data collection to simulation as the grade level went higher. The first three data related CT components were dominating at elementary level, all components of CT except parallelization were found at middle school level, and finally more frequencies in every component of CT except parallelization were also found at high school level than middle school level. On the basis of the result of CT usage in STEAM programs, we included ‘generalization’ in CTPF of science education instead of ‘parallelization’ which was not found. The implication about teacher education was made based on the CTPF in terms of science education.

19세기말 Mycobacterium avium subsp. paratuberculosis(Map)이 요네병(Johne’s disease)의 원인균임이 밝혀진 이후, 불현성 감염된 동물들의 국제적 이동은 요네병을 전 세계로 퍼뜨리기 시작하였다. 이러한 요네병이 축산분야에 나타남과 동시에 새로운 형태의 대장염으로서 요네병과 같은 증상을 나타내는 질병(크론병)이 사람에게서도 나타나기 시작하였다. 그러나 Map이 이러한 새로운 대장염의 원인균이며 인수공통전염병의 원인체라는 인식은 20세기 후반 이러한 질병을 앓고 있는 사람의 조직으로부터 Map을 검출할 수 있게 되고서야 나타나기 시작했다. 본 총설은 어떻게 Map이 축산분야와 사람의 공중보건 측면 에서 심각한 문제를 야기시키게 되었고, Map 감염에 의한 대장염 환자의 치료가 어떻게 발전되어 왔는지 간단히 요약하고, 축산에서 Map의 통제를 위한 새로운 백신개발 전략에 대하여 소개한다.