This study was performed to enhance anticancer activities of Lithospermum erythrorhizon by eluting high amount of shikonin through ultra high pressure process. Extraction yield was increased up to 5~10% by ultra high pressure process, compare to the normal extraction processes such as water solvent extraction, 70% ethyl alcohol solvent extraction. The cytotoxicity of the extracts (1.0μg/ml) from ultra high pressure process was showed the lowest cytotoxicity 13.4% for human lung cell (HEL299). The anticancer activities showed 80~85% by adding 1.0μg/ml of the extracts from ultra high pressure process in several cancer cell lines such as AGS, Hep3B, MCF-7 and HeLa cells. Among them, MCF-7 cell of the endocrine system was highest inhibited than other cells. The anticancer activities of the extracts from ultra high pressure extraction process showed 10~15%, which was higher than the extracts from normal extraction processes. From HPLC analysis of the extracts, the contents of shikonin in the extracts from ultra high pressure process was 11.42% (w/w), which was 20% higher than others. This results indicate that ultra high pressure process could increase the extraction yield of shikonin and other contents, which resulted in higher anticancer activities.

In this study, whitening activity of Lithospermum erythrorhizon extracts were investigated according to several extraction processes: water extraction at 100℃ (WE100) and 60℃ (WE60), 70% ethyl alcohol extraction (EE) and ultra high pressure extraction (HPE) at 500 MPa for 30 minutes at 60℃. The extracts from ultra high pressure extraction showed the highest tyrosinase inhibition and melanogenesis inhibition activities as 52% and 79.5%, respectively, in adding 1mg/ml than others extraction processes. HPE extracts also showed the strong reducing power as 3.19 that absorbance at 450 mm. The contents of polyphenol in WE100, we measured as 10.1μg/ml in adding 1mg/ml. Extracts have a high total flavonoid contents by HPE as 4.1μg/ml at 1mg/ml. We can conclude that better whitening activity of extracts from high pressure extraction was due to high antioxidant activities which could be extracted by higher polyphenol and flavonoid contents in HPE than others.

This study was performed to investigate the enhancement of cosmeceutical activities of Berberis koreana bark by different extraction processes. The extracts are WE (water extract at 100℃, control), USE (ultrasonification for 1 hours at 60℃ with water), HPE (high pressure for 5 minutes at 60℃ with water) and USE + HPE (ultrasonification process for 1 hours after high pressure for 5 minutes at 60℃ with water), respectively. The cytotoxicity of the extracts was in the range of 24.02~26.94% at 1.0 mg/ml concentration. The USE + HPE showed the lowest cytotoxicity. Compared to the WE, total phenolic and flavonoid contents in the USE + HPE increased to 121.5% and 154.2%. The USE + HPE showed the highest activity at 1.0 mg/ml concentration in 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging, inhibition activity of xanthine oxidase and superoxide dismutase (SOD)-like test, respectively. Tyrosinase inhibition of WE, USE, HPE and USE + HPE at 1.0 mg/ml concentration was measured as 17.72, 19.62, 22.83 and 24.16%, respectively. Hyaluronidase inhibition activities of the USE + HPE were higher than 20.8%~29.5% of the WE. Our results suggested that the extracts from ultrasonification process after high pressure extraction has relatively high cosmeceutical activities, and that the bark of Berberis koreana could be considered as a candidate of new functional cosmetic agents.

The bark of Berberis koreana Palibin was fermented by Bifidobacterium longum B6 and Lactobacillus paracasei at 37℃ for 72 hour, then extracted by water solvent at 100℃ for 180 min. Total pholyphenol and flavonoid contents were improved by fermentation process, compared to conventional water extraction. The barks of B. koreana Palibin extracts by B. longum B6 (FE-B.L.) and by L. paracasei (FE-L.P.) showed 17% and 16% cytotoxicity on human normal cell lines(HEK293) at 1.0 mg/ml of the highest concentration, respectively, which was about 3~5% lower than 20% from normal extracts (NE). DPPH radical scavenging activity of the FE-B.L. and FE-L.P. were about 73% and 75.9% higher than 56.8% of NE. The highest inhibitory potency on xathine oxidase and superoxide dismutase (SOD)-like activities were also measured as 31.9% and 61.9% by adding FE-L.P. of 1.0 mg/ml. On tyrosinase inhibition test, the FE-L.P. showed highest activity as 75.9% at 1.0 mg/ml. Generally, FE-L.P. showed higher antioxidant activities as well as higher tyrosinase inhibition activity, possibly due to high contents of total pholyphenol and flavonoid. In general, fermentation of barks of B. koreana Palibin has relatively better biological activities than normal extracts. Specifically, the extracts fermented by L. paracasei showed higher activities than that from B. longum B6.

This work was to improve antimicrobial activities of horseradish by encapsulated with edible biopolymers such as lecithin and gelatin since it has been difficult to directly use horseradish extracts into foods and food containers due to its strong and undesirable flavors. It was shown that most of the nanoparticles containing the extracts were well formed in round shape with below 400 nm diameter as well as fairly stable and less odd flavors in various pH ranges by measuring zeta potentials. The encapsulation efficiencies of nanoparticles were estimated as 66.6% and 53.4% for lecithin and gelatin, respectively. Minimal Inhibitory Concentration (MIC) of both nanoparticles against G(+), Listeria monocytogenes and G(-), Salmonella typhimurium were also measured as 79 ppm based on AIT concentrations in the extracts, whose activities were about 65% higher than the case of adding crude extract. It was also found that the nanoparticles efficiently penetrated into the cell membrane and started to destruct the cells after 6 hours cultivation under Transmision Electron Microscopy observation. These results prove that the nano-encapsulation of the horseradish extracts can be employed to directly treat into the foods and food containers for antimicrobial purposes with the aids of aerosolization system, by using small amounts of the extracts and having less flavors due to masking effects of nanoparticles.

We investigated a method to improve anticancer activities of Acer mono wood extracts by ultra high pressure extraction process. The A. mono was extracted by water at 40℃ and 300 MPa for 15 min (High Pressure Extraction, HPE). The extraction yield by ultra high pressure extraction process was 5.42%. The cytotoxicity on human normal lung cell (HEL299) of the extracts from HPE showed 21.54% lower than that from conventional water extraction at 100℃ in adding the maximum concentration of 1.0 mg/ml. Ultra high pressure extracts process for 15 minutes extracts (HPE15) showed more potent scavenging effect than the control, BHA. On SOD-like test, the HPE15 showed highest activity as 32.4% at 1.0 mg/ml concentration. Human stomach adenocarcinoma, liver adenocarcinoma, breast adenocarcinoma and lung adenocarcinoma cell growth were inhibited up to about 67~79%, in adding 1.0 mg/ml of extracts from HPE. HPE was 20~25% higher than conventional water extraction. It was interesting that, among several cancer cell lines (stomach adenocarcinoma, liver adenocarcinoma), the growth of digestive related cancer cells were most effectively inhibited as about 75~79%. On in vivo experiment using ICR mice, the variation of body weight of mice group treated A. mono wood extracts from HPE of 100 mg/kg/day concentration was very lower than control and other group. The survival times of group treated this extracts was 61.96% longer than that of the control group and this extracts showed the lower tumor weight, which were 10.49 g than positive control as 16.17 g. Based on these results, we could tell that the HPE wood extracts of A. mono had higher anticancer activity than conventional water extraction. The results of HPE showed obvious advantages in higher efficiency, shorter extraction time, at lower energy costs.

This study showed the increase of antitumor activities of water soluble E. sinica extract by nano-encapsulation process with lecithin. Five groups of lecithin only group (LO), lecithin nano-encapsulated E. sinica group (LE), E. sinica only group (EO), one negative control group (NCO) and positive control group (PCO) were set for several anticancer experiment and fed into Sarcoma-180 injected mice. The cytotoxicity of LE on the human normal kidney cell (HEK293) showed 14.8% lower than 19.2% of EO and 18.4% of LO. Growth of human liver carcinoma cell and human stomach carcinoma cell as representative of digestive system in vitro was inhibited up to about 85.1% and 87.3%, in adding 1.0 mg/ml of LE, which values 15% higher than that from conventional EO. The survival rates of each mice group were 40%, 63%, 48%, 33% and 100%, respectively after 40 days of injecting Sarcoma-180. The increment of their body weights of the extract feeding groups was suppressed down to 10~15%, compared to the negative control. The nano-particles also reduced the hypertrophy of the internal organs such as spleen and liver down to 15~20%, compared to those as the other groups. Among them, LE effectively reduced the size of tumor form to 20%. From these results, in vitro and in vivo antitumor activities of E. sinica could be enhanced by using nano-encapsulation process with lecithin because of better permeation into the cancer cells by confocal observations.

Low quality fresh ginseng was fermented by Pheliuus linteus mycelium at 22℃ for 30 days, then extracted by water solvent at 100℃ for 180 min. On human normal cell lines (HEK293), cytotoxicity was about 10% lower in adding extracts of the fermentation ginseng than that from low quality ginseng. The fermented extracts also inhibited the growth of several human cancer cells. Among them, respectively, digestive organs related cancer cells, such as human stomach adenocarcnioma and human epithelial adenocarcinoma were most effectively inhibited up to 85% and 90%, respectively. Then, selectivities were in the ranges of 3 to 5, compared to 2 to 3 from low quality fresh ginseng. Generally, fermented ginseng extract showed higher anticancer activities as well as higher DPPH radical sacavening activity, possibly due to high contents of total phenolic components as 6.96 mg/g. It was very interesting that the fermented ginseng contained very higher contents of ginsenoside-Rc+Rb2, compared to others in low quality fresh ginseng because of partition digestion of mycelium growth. The results can tell that low quality fresh ginseng can be utilized by the fermentation with Pheliuus linteus mycelium.

｢심청전｣과 관련을 맺고 있는 설화 중에는 이 작품의 형성에 직접․간접으로 영향을 끼친 배경 설화가 있다. 배경 설화에는 태몽(胎夢) 설화, 효행(孝行) 설화, 인신공희(人身供犧) 설화, 재생(再生) 설화, 개안(開眼) 설화가 있다. ｢심청전｣의 배경 설화 중 ｢관음사 연기 설화｣는 문학성이 뛰어난 작품인데, ｢심청전｣의 형성에 영향을 끼쳤을 것이다. 심청을 주인공으로 하여 그녀의 효행, 죽음과 재생을 이야기하는 ‘심청 설화’에는 백령도 지방에 전해 오는 ｢심청 전설｣과 동해안 지방에 전해 오는 ｢심청굿 무가｣가 있다. ｢심청굿 무가｣는 활자본 ｢심청전｣의 영향을 받아서 형성된 것이다. 백령도 지역의 ｢심청 전설｣은 ｢심청전｣을 바탕으로 하여 형성된 전설이다. ｢심청전｣의 이본과 판소리 ｢심청가｣를 보면, 심청이 나서 자란 곳은 황주 도화동이다. 그리고 심청이 죽었다가 살아난 곳은 장산곶과 백령도 사이에 있는 물살이 세기로 이름난 ‘인당수’이다. 그러므로 ｢심청전｣의 배경이 된 곳은 황해도 황주에서 장산곶과 인당수를 지나 백령도 연봉, 연화리를 잇는 지역이 된다.

본 논문에서는 인터넷환경에서 증가하는 인터넷게임의 바이러스/웜의 공격에 의한 침해사고 발생시 정의되어야 할 정보와 이를 활용한 대량 트래픽을 발생시키는 탐지하는 방안을 제안하였다. 이에 따라 인터넷 게임 사고대응시스템에서의 자동화된 역추적 방안에 대한 설계와 기능에 대한 개념을 제시한다.

오늘날 배의 크기를 나타내는 단위로 톤(ton)을 사용하고 있다. 톤이란 용어가 배의 크기를 나타내는 일반명사가 되기 이전에 세계 여러 지역에서는 고유한 단위로 배의 크기를 나타내었다. 이를테면 서유럽에서는 포도주 통인 ton, 북유럽에서는 곡량 단위인 last, 제노바에서 는 cantara, 베네치아에서는 milliaria, 나폴리에서는 carra를 각각 사용했다. 동양권에서는 가장 중요한 화물이 쌀이었기 때문에 한중일 모두 미량 단위인 을 사용하여 배의 크기를 나타냈다. 특히 중국인들은 싸라는 독특한 단위를 사용하기도 하였다. 이처럼 각 지역과 시기에 따라 다른 단위를 사용하여 배의 크기를 나타냈으나, 19세기 중반 Moorsom 방식에 의한 톤수 측정법이 도입되고, 이것이 전세계적으로 보급됨에 따라 톤이 배의 크기를 나타내는 단위로 정착하게 되었다.

일본의 구법승 엔닌은 견당사선에 동승하여 입당한 뒤 서기 838년 6월 13일부터 847년 12월 14일까지 당에 머무르면서 9년 반에 걸친 행적을 『입당구법순례행기』라는 일기에 남겼다. 이 일기에는 당시 당의 사회, 경제, 종교, 외교, 관사, 지리, 교통 및 행정에 대한 방대한 정보가 포함되어 있다. 특히 이 일기의 초입두분 즉 838년 6원 13일부터 7월 3일의 기록은 마치 항해일지와 같은 방식으로 기록되어있다. 여기에 나타나 있는 항해 및 조선 관련 용어들은 당시 고대선과 항해기술을 연구하는데 대단히 중요한 단서가 된다. 이 논문에서는 『입당구법순례행기』에 등장하는 ‘누아’가 어떤 구성재 인지를 구명하고자 한다. 이 연구는 고대선박의 구조에 대한 이해를 높이는데 기여할 것이다.

이 논문에서 필자들은 유럽과 중국에서 거의 비슷한 시기에 나침반이 항해에 이용되었음을 역사적 기록과 최근까지의 연구성과들을 검토함으로써 밝히고자 하였다. 그 연구 결과는 다음과 같다. 첫째, 동서양 문명권에서 항해자들이 나침반을 항해에 이용하기까지 비슷한 과정을 거쳤다. 즉 표침, 축침, 콤파스 카드 순으로 항해용 나침반을 개발하여 사용하였다. 둘째, Frode의 사가(saga)를 고려해 볼 때 중국과 유럽에서는 11세기 경에 나침반을 항해에 이용하였다. 결론적으로 중국인들이 세계 최초로 나침반을 항해에 이용했다는 기존 주장과는 달리, 중국인들과 유럽인들은 거의 비슷한 시기에 서로 독자적으로 나침반을 항해에 이용하였다.

지금까지 중학교 국어과 교육 과정을 교수요목기, 제1,2,3,4,5차 교육 과정기로 나누고, 제2장에서는 각 시기별 교육과정의 개요 및 특성을 살펴 보았고, 제3장에서는 국어과 교육 과정의 체제, 목표, 내용, 지도상의 유의점, 시간 배당 기준 등의 역사적 변천을 살펴보았다. 그리고, 제6장에서는 이를 통해서 드러난 문제점과 개선 방향을 정리하여 제시 하였다. 국어과 교육 과정은 개편을 거듭하는 동안 미비점과 문제점이 보완, 개선되어 온 것은 사실이다. 그러나, 제6장에서 지적한 것과 같은 문제점이나 개선점은 아직도 남아 있다. 이것은 앞으로 보완, 개선되어야 하리라 생각한다.국어과 교육 과정과 함께 국어과 교육에 영향을 미치는 것은 국어 교과서이다. 국어교과서를 국정으로만 묶어 둘 것이 아니라 검인정으로 하여 교육 과정의 정신과 내용을 충실히 살릴 수 있는 여러 종류의 교과서가 나오도록 해야 하리라 생각한다. 그래야만, 지역적 특성을 고려한 교과서도 개발될 수 있을 것이라 생각한다.